公开(公告)号：US20210292813A1

公开(公告)日：2021-09-23

申请号：US17322737

申请日：2021-05-17

申请人： Multilex, Inc.

发明人： Yong Tae Kim ,  Jun Hye Moon

IPC分类号： C12Q1/686 ,  C12Q1/6876 ,  C12Q1/6853

摘要： According to an exemplary embodiment of the present application, provided is a nucleic acid complex pair, which includes a first nucleic acid complex including a first determinant and a second tag; and a second nucleic acid complex including a second determinant and a second tag, wherein the first determinant includes a forward primer for first target DNA, the second determinant includes a reverse primer for the first target DNA, the first tag includes a base sequence complementary to the base sequence of the second tag, and the second tag includes a base sequence complementary to the base sequence of the first tag.

公开(公告)号：US11118228B2

公开(公告)日：2021-09-14

申请号：US15881409

申请日：2018-01-26

申请人： Exact Sciences Development Company, LLC ,  Mayo Foundation for Medical Education and Research

发明人： Hatim T. Allawi ,  Michael W. Kaiser ,  Graham P. Lidgard ,  William R. Taylor ,  Tamara J. Sander ,  Abram M. Vaccaro

IPC分类号： C12Q1/6883 ,  C12Q1/6886 ,  G01N33/533 ,  G16Z99/00 ,  C12Q1/6809 ,  C12Q1/6827 ,  C12Q1/6853 ,  G01N33/574

摘要： Provided herein is technology for neoplasia screening, and particularly, but not exclusively, to methods, compositions, and related uses for detecting the presence of cancer, in particular, colorectal cancer.

公开(公告)号：US11117113B2

公开(公告)日：2021-09-14

申请号：US15382360

申请日：2016-12-16

申请人： Fluidigm Corporation

发明人： Peilin Chen

IPC分类号： C12Q1/6853 ,  C12Q1/6848 ,  C12Q1/6844 ,  B01J19/00

摘要： The present disclosure provides a “looping amplification” method to increase the specificity of nucleic acid amplification. This increased specificity facilitates multiplexing to a much higher degree than was previously possible.

公开(公告)号：US20210269868A1

公开(公告)日：2021-09-02

申请号：US17187267

申请日：2021-02-26

申请人： CFD Research Corporation

发明人： David Edwin Gaddes, III ,  Ketan Harendrakumar Bhatt ,  Balabhaskar Prabhakarpandian

IPC分类号： C12Q1/6853 ,  C12Q1/6832 ,  C12Q1/6816

摘要： A primer composition for amplifying a target nucleic acid of a plurality of target organisms can include: a first primer having in order: 3′-a first target specific sequence; a melt key region; and a first super primer region, wherein the first target specific sequence includes a nucleic acid sequence that specifically hybridizes with a first unique nucleic acid sequence of a first target organism over nucleic acid sequences of other organisms, the melt key region includes a nucleic acid sequence that has less than full identity with a melt key probe and a complement of the melt key region hybridizes with the melt key probe with at least one nucleotide mismatch with the melt key probe, and the first super primer region includes a nucleic acid sequence that is common to the plurality of target organisms.

公开(公告)号：US11098371B2

公开(公告)日：2021-08-24

申请号：US16460546

申请日：2019-07-02

申请人： National Central University ,  Huan-Cheng Chang ,  DELTA ELECTRONICS, INC.

发明人： Nian-Han Ma ,  Chien-Lung Chen ,  Chen-Huan Lin ,  An-Lun Li ,  Chiu-Chin Huang ,  Chao-Hsiang Chang

IPC分类号： C12Q1/6886 ,  C12Q1/6837 ,  C12Q1/6853

摘要： A kit for detecting urothelial carcinoma is provided. The kit includes at least one primer or probe for detecting one or more miRNAs, and the one or more miRNAs are selected from the group consisting of miR-19b-1, miR-636, miR-150, miR-155, miR-183, miR-378, miR-210, miR-487, and combinations thereof. A method for detecting urothelial carcinoma is also provided. The method includes detecting the expression level(s) of one or more miRNAs in a sample of a subject, and the one or more miRNAs are selected from the group consisting of miR-19b-1, miR-636, miR-150, miR-155, miR-183, miR-378, miR-210, miR-487, and combinations thereof.

公开(公告)号：US11091802B2

公开(公告)日：2021-08-17

申请号：US16045566

申请日：2018-07-25

申请人： Multilex, Inc.

发明人： Yong Tae Kim ,  Jun Hye Moon

IPC分类号： C12Q1/686 ,  C12Q1/6876 ,  C12Q1/6853

摘要： The present invention relates to a nucleic acid complex pair for detecting a target nucleic acid in a sample, and more particularly to a nucleic acid complex pair used for detecting a target DNA in a sample, wherein the nucleic acid complex pair includes a first nucleic acid complex including a first determination region, a first pairing region, and a first detection region; and a second nucleic acid complex including a second determination region, a second paring region, and a second detection region, wherein the first determination region includes at least a partial domain that complementarily binds to a first target nucleic acid sequence, and the second determination region includes at least a partial domain that complementarily binds to a second target nucleic acid sequence, wherein the first pairing region and the second pairing region a domain where they can complementarily hybridize to each other.

公开(公告)号：US20210222233A1

公开(公告)日：2021-07-22

申请号：US17207040

申请日：2021-03-19

申请人： Arizona Board of Regents on Behalf of the University of Arizona

发明人： Ankit Desai

IPC分类号： C12Q1/6827 ,  C12Q1/6883 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6816 ,  C12Q1/6851 ,  C12Q1/6853 ,  C12Q1/6858

摘要： The present invention relates to compositions and methods for diagnosing and treating arrhythmias. In particular, the present invention provides IL-18 markers and uses thereof.

公开(公告)号：US11066700B2

公开(公告)日：2021-07-20

申请号：US16752277

申请日：2020-01-24

申请人： Multilex, Inc.

发明人： Yong Tae Kim ,  Jun Hye Moon

IPC分类号： C12Q1/686 ,  C12Q1/6876 ,  C12Q1/6853

摘要： According to an exemplary embodiment of the present application, provided is a nucleic acid complex pair, which includes a first nucleic acid complex including a first determinant and a second tag; and a second nucleic acid complex including a second determinant and a second tag, wherein the first determinant includes a forward primer for first target DNA, the second determinant includes a reverse primer for the first target DNA, the first tag includes a base sequence complementary to the base sequence of the second tag, and the second tag includes a base sequence complementary to the base sequence of the first tag.

公开(公告)号：US20210198662A1

公开(公告)日：2021-07-01

申请号：US17198140

申请日：2021-03-10

申请人： GRAIL, Inc.

发明人： Matthew Larson

IPC分类号： C12N15/10 ,  C40B40/06 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q1/6855

摘要： Aspects of the invention include methods for preparing sequencing libraries comprising a plurality of RNA molecules. In some embodiments, the subject methods comprise sequencing at least a portion of a sequencing library to obtain sequencing data or sequence reads from a test sample (e.g., a biological sample from a subject).

公开(公告)号：US20210171999A1

公开(公告)日：2021-06-10

申请号：US17096719

申请日：2020-11-12

申请人： General Automation Lab Technologies Inc.

发明人： Alexander HALLOCK ,  Kimberly WEMMER

IPC分类号： C12Q1/04 ,  C12M1/32 ,  C12Q1/6853 ,  C12Q1/689 ,  C12Q1/6816 ,  B01L3/00 ,  C12M1/04 ,  B01L3/02

摘要： A microfabricated device defining a high density array of microwells is described for cultivating cells from a sample. The device may be incubated to grow a plurality of cells, which may be split into an analysis portion and a reserve portion. Methods are provided for screening a biological entity of interest using the microfabricated device, for example, for screening phosphate solubilizing bacteria or other bacteria producing acids.