The present invention relates to a nucleic acid complex pair for detecting a target nucleic acid in a sample, and more particularly to a nucleic acid complex pair used for detecting a target DNA in a sample, wherein the nucleic acid complex pair includes a first nucleic acid complex including a first determination region, a first pairing region, and a first detection region; and a second nucleic acid complex including a second determination region, a second paring region, and a second detection region, wherein the first determination region includes at least a partial domain that complementarily binds to a first target nucleic acid sequence, and the second determination region includes at least a partial domain that complementarily binds to a second target nucleic acid sequence, wherein the first pairing region and the second pairing region a domain where they can complementarily hybridize to each other.