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    COMPOSITIONS AND METHODS FOR DIFFERENTIAL REGULATION OF FATTY ACID UNSATURATION IN MEMBRANE LIPIDS AND SEED OIL
    81.
    发明申请
    COMPOSITIONS AND METHODS FOR DIFFERENTIAL REGULATION OF FATTY ACID UNSATURATION IN MEMBRANE LIPIDS AND SEED OIL 审中-公开
    膜脂和种子油中脂肪酸不饱和度差异调节的组合物和方法

    公开(公告)号：US20160272985A1

    公开(公告)日：2016-09-22

    申请号：US14948166

    申请日：2015-11-20

    申请人： Washington State University

    发明人： John A. Browse ,  Chaofu Lu ,  Zhanguo Xin

    IPC分类号： C12N15/82 ,  A23D9/013 ,  A01H5/10 ,  C10L1/02 ,  C12N9/12 ,  C11B1/00

    CPC分类号： C12N15/8247 ,  A01H5/10 ,  A23D9/013 ,  C10L1/026 ,  C10L2200/0476 ,  C10L2270/026 ,  C11B1/00 ,  C12N9/1288 ,  C12Y207/08002

    摘要： Aspects of the invention provide methods for differential regulation of fatty acid unsaturation in seed oil and membrane lipids of plants based on modulation of a previously unknown biosynthetic pathway involving a novel phosphatidylcholine:diacylglycerol cholinephosphotransferase (PDCT) that regulates phosphatidylcholine biosynthesis in developing oil seed plants. Specific aspects relate to inventive PDCT polypeptides including, for example, variants, deletions, muteins, fusion proteins, and orthologs thereof (collectively PDCT proteins), to nucleic acids encoding same, to plants comprising such PDCT sequences or proteins or devoid or depleted of such PDCT proteins or sequences, and to methods for generating plants having altered or no PDCT expression and/or activity, including but not limited to methods comprising mutagenesis, recombinant DNA, transgenics, etc.

    摘要翻译： 本发明的方面提供了基于调节以前未知的涉及新型磷脂酰胆碱的生物合成途径的调节脂肪酸不饱和度的方法：在开发油种子植物中调节磷脂酰胆碱生物合成的二酰基甘油胆碱磷酸转移酶（PDCT）。 具体方面涉及本发明的PDCT多肽，包括例如变体，缺失，突变蛋白，融合蛋白及其直系同源物（统称为PDCT蛋白质）与编码该核酸的核酸组合在一起，包含这样的PDCT序列或蛋白质的植物或缺乏或缺失的 PDCT蛋白或序列，以及产生具有改变或不具有PDCT表达和/或活性的植物的方法，包括但不限于包含诱变，重组DNA，转基因等的方法。

    Production of acetyl-coenzyme a derived isoprenoids
    86.
    发明授权
    Production of acetyl-coenzyme a derived isoprenoids 有权
    生产乙酰辅酶A衍生的类异戊二烯

    公开(公告)号：US08859261B2

    公开(公告)日：2014-10-14

    申请号：US14062798

    申请日：2013-10-24

    申请人： Amyris, Inc.

    发明人： Timothy Stevens Gardner ,  Kristy Michelle Hawkins ,  Adam Leon Meadows ,  Annie Ening Tsong ,  Yoseph Tsegaye

    IPC分类号： C12P5/00 ,  C12P1/02 ,  C12N1/00 ,  C12P23/00 ,  C12N15/52 ,  C12N9/04 ,  C12N9/02 ,  C12N9/12 ,  C12N15/81 ,  C12P7/42

    CPC分类号： C12P5/007 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1288 ,  C12N15/52 ,  C12N15/81 ,  C12P7/42 ,  C12P23/00 ,  C12Y101/01034 ,  C12Y102/0101 ,  C12Y207/08

    摘要： Provided herein are compositions and methods for the heterologous production of acetyl-CoA-derived isoprenoids in a host cell. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an acetaldehyde dehydrogenase, acetylating (ADA, E.C. 1.2.1.10) and an MEV pathway comprising an NADH-using HMG-CoA reductase. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleotide sequence encoding an ADA and an MEV pathway comprising an acetoacetyl-CoA synthase. In some embodiments, the genetically modified host cell further comprises one or more heterologous nucleotide sequences encoding a phosphoketolase and a phosphotransacetylase. In some embodiments, the genetically modified host cell further comprises a functional disruption of the native PDH-bypass. The compositions and methods described herein provide an energy-efficient yet redox balanced route for the heterologous production of acetyl-CoA-derived isoprenoids.

    摘要翻译： 本文提供了在宿主细胞中异源产生乙酰辅酶A衍生的类异戊二烯的组合物和方法。 在一些实施方案中，宿主细胞被遗传修饰以包含编码乙醛脱氢酶，乙酰化（ADA，E.C.1.2.1.10）和包含使用NADH的HMG-CoA还原酶的MEV途径的异源核苷酸序列。 在一些实施方案中，宿主细胞被遗传修饰以包含编码包含乙酰乙酰辅酶A合成酶的ADA和MEV途径的异源核苷酸序列。 在一些实施方案中，经遗传修饰的宿主细胞还包含一个或多个编码磷酸酮醇酶和磷酸转乙酰酶的异源核苷酸序列。 在一些实施方案中，遗传修饰的宿主细胞还包括天然PDH旁路的功能性破坏。 本文描述的组合物和方法提供了用于异源产生乙酰辅酶A衍生的类异戊二烯的能量效率尚未的氧化还原平衡途径。

    NOVEL METHOD FOR UTILIZATION OF MICROBIAL MUTANT
    88.
    发明申请
    NOVEL METHOD FOR UTILIZATION OF MICROBIAL MUTANT 审中-公开
    用于微生物突变体的新方法

    公开(公告)号：US20110318792A1

    公开(公告)日：2011-12-29

    申请号：US12377230

    申请日：2007-08-14

    申请人： Toshio Ohsuga ,  Kuni Fushikida

    发明人： Toshio Ohsuga ,  Kuni Fushikida

    IPC分类号： C12P13/12 ,  C12N1/19 ,  C12N1/15

    CPC分类号： C12P19/40 ,  C12N9/1288

    摘要： The object of the present invention is to provide a microbial mutant capable of producing S-adenosylmethionine (hereinafter referred to as SAM) in a large amount with no change in growth characteristics and a method of efficiently producing SAM by incubating the microbial mutant.The method of producing S-adenosylmethionine comprises incubating a microorganism having a loss or a reduction in the function of an enzyme relating to synthesis of phosphatidylcholine and recovering S-adenosylmethionine accumulated in the culture.

    摘要翻译： 本发明的目的是提供一种生长特性没有变化的能够大量产生S-腺苷甲硫氨酸（以下称为SAM）的微生物突变体，以及通过温育微生物突变体有效地生产SAM的方法。 生产S-腺苷甲硫氨酸的方法包括将与磷脂酰胆碱合成有关的酶的功能丧失或降低的微生物孵育并回收在培养物中积累的S-腺苷甲硫氨酸。

    VEGETABLE OILS AND USES THEREFOR
    89.
    发明申请
    VEGETABLE OILS AND USES THEREFOR 有权
    蔬菜油及其用途

    公开(公告)号：US20110229623A1

    公开(公告)日：2011-09-22

    申请号：US13011779

    申请日：2011-01-21

    申请人： Qing Liu ,  Allan Graham Green ,  Surinder Pal Singh

    发明人： Qing Liu ,  Allan Graham Green ,  Surinder Pal Singh

    IPC分类号： A23D9/00 ,  A01H5/10 ,  A23D9/02

    CPC分类号： C12N15/8247 ,  A23D9/00 ,  C11B1/10 ,  C12N9/1029 ,  C12N9/1288 ,  C12N15/8218 ,  C12P7/6463 ,  C12P7/6472

    摘要： The specification relates to plants and their seeds and oil obtained therefrom, and to methods of producing same comprising oil having modified fatty acid compositions, such that 28% to 80% of the total fatty acid content in the seedoil is palmitic acid, 0% to 16% is palmitoleic acid, 0% to 4% is C16:2 fatty acid, 3% to 33% is stearic acid, 1% to 40% is oleic acid, 4% to 50% is linoleic acid and 0% to 10% is linolenic acid. The specification describes nucleic acid molecules encoding RNA capable of conferring these properties, in particular, RNA that inhibits expression of an oil biosynthesis gene encoding KASII in seeds of a plant. Genetic constructs and cells comprising the nucleic acid molecules are also described and claimed.

    摘要翻译： 本说明书涉及植物及其种子，由此得到的油，以及含有改性脂肪酸组合物的油的制造方法，使得种子油中总脂肪酸含量的28〜80％为棕榈酸，0％〜 16％为棕榈油酸，0％〜4％为C16：2脂肪酸，3％〜33％为硬脂酸，1％〜40％为油酸，4％〜50％为亚油酸，0％〜10％ 是亚麻酸。 该说明书描述了编码能够赋予这些性质的RNA的核酸分子，特别是抑制植物种子中编码KASII的油生物合成基因表达的RNA。 还描述和要求保护包含核酸分子的遗传构建体和细胞。

    COMPOSITIONS AND METHODS FOR DIFFERENTIAL REGULATION OF FATTY ACID UNSATURATION IN MEMBRANE LIPIDS AND SEED OIL
    90.
    发明申请
    COMPOSITIONS AND METHODS FOR DIFFERENTIAL REGULATION OF FATTY ACID UNSATURATION IN MEMBRANE LIPIDS AND SEED OIL 有权
    膜脂和种子油中脂肪酸不饱和度差异调节的组合物和方法

    公开(公告)号：US20110131678A1

    公开(公告)日：2011-06-02

    申请号：US12921119

    申请日：2009-03-04

    申请人： John A. Browse ,  Chaofu Lu ,  Zhanguo Xin

    发明人： John A. Browse ,  Chaofu Lu ,  Zhanguo Xin

    IPC分类号： A01H1/06 ,  C12N15/82 ,  A01H5/00 ,  C07H21/00 ,  C12N9/12 ,  C12N5/10 ,  C07C57/02

    CPC分类号： C12N15/8247 ,  A01H5/10 ,  A23D9/013 ,  C10L1/026 ,  C10L2200/0476 ,  C10L2270/026 ,  C11B1/00 ,  C12N9/1288 ,  C12Y207/08002

    摘要： Aspects of the invention provide methods for differential regulation of fatty acid unsaturation in seed oil and membrane lipids of plants based on modulation of a previously unknown biosynthetic pathway involving a novel phosphatidylcholine: diacylglycerol cholinephosphotransferase (PDCT) that regulates phosphatidylcholine biosynthesis in developing oil seed plants. Specific aspects relate to inventive PDCT polypeptides including, for example, variants, deletions, muteins, fusion proteins, and orthologs thereof (collectively PDCT proteins), to nucleic acids encoding same, to plants comprising such PDCT sequences or proteins or devoid or depleted of such PDCT proteins or sequences, and to methods for generating plants having altered or no PDCT expression and/or activity, including but not limited to methods comprising mutagenesis, recombinant DNA, transgenics, etc.

    摘要翻译： 本发明的方面提供了基于调节以前未知的涉及新型磷脂酰胆碱的生物合成途径的调节脂肪酸不饱和度的方法：在开发油种子植物中调节磷脂酰胆碱生物合成的二酰基甘油胆碱磷酸转移酶（PDCT）。 具体方面涉及本发明的PDCT多肽，包括例如变体，缺失，突变蛋白，融合蛋白及其直系同源物（统称为PDCT蛋白质）与编码该核酸的核酸组合在一起，包含这样的PDCT序列或蛋白质的植物或缺乏或缺失的 PDCT蛋白或序列，以及产生具有改变或不具有PDCT表达和/或活性的植物的方法，包括但不限于包含诱变，重组DNA，转基因等的方法。