-
公开(公告)号：US10287600B2
公开(公告)日：2019-05-14
申请号：US14948166
申请日：2015-11-20
发明人： John A. Browse , Chaofu Lu , Zhanguo Xin
摘要： Aspects of the invention provide methods for differential regulation of fatty acid unsaturation in seed oil and membrane lipids of plants based on modulation of a previously unknown biosynthetic pathway involving a novel phosphatidylcholine:diacylglycerol cholinephosphotransferase (PDCT) that regulates phosphatidylcholine biosynthesis in developing oil seed plants. Specific aspects relate to inventive PDCT polypeptides including, for example, variants, deletions, muteins, fusion proteins, and orthologs thereof (collectively PDCT proteins), to nucleic acids encoding same, to plants comprising such PDCT sequences or proteins or devoid or depleted of such PDCT proteins or sequences, and to methods for generating plants having altered or no PDCT expression and/or activity, including but not limited to methods comprising mutagenesis, recombinant DNA, transgenics, etc.
-
2.发明申请DESATURASES AND METHODS OF USING THEM FOR SYNTHESIS OF POLYUNSATURATED FATTY ACIDS 有权使用它们合成聚氧乙烯脂肪酸的苦味和方法公开(公告)号：US20140298502A1
公开(公告)日：2014-10-02
申请号：US14308456
申请日：2014-06-18
CPC分类号： C12P7/6409 , C12N9/0071 , C12N9/0083 , C12N15/80 , C12N15/8241 , C12N15/8247 , C12N15/85
摘要： The amino acid and nucleic acid sequences of a Δ5-desaturase enzyme and a Δ8-desaturase enzyme are disclosed. The nucleic acid sequences can be used to design recombinant DNA constructs and vectors. These vectors can then be used to transform various organisms, including for example, plants and yeast. The transformed organisms will then produce polyunsaturated fatty acids. The amino acid sequences are useful for generating enzyme-specific antibodies that are useful for identifying the desaturases.
摘要翻译： 公开了＆Dgr.5-去饱和酶和＆Dgr.8-去饱和酶的氨基酸和核酸序列。 核酸序列可用于设计重组DNA构建体和载体。 然后可以将这些载体用于转化各种生物体，包括例如植物和酵母。 转化的生物将产生多不饱和脂肪酸。 氨基酸序列可用于产生可用于鉴定去饱和酶的酶特异性抗体。
-
公开(公告)号：US09695450B2
公开(公告)日：2017-07-04
申请号：US14308456
申请日：2014-06-18
CPC分类号： C12P7/6409 , C12N9/0071 , C12N9/0083 , C12N15/80 , C12N15/8241 , C12N15/8247 , C12N15/85
摘要： The amino acid and nucleic acid sequences of a Δ5-desaturase enzyme and a Δ8-desaturase enzyme are disclosed. The nucleic acid sequences can be used to design recombinant DNA constructs and vectors. These vectors can then be used to transform various organisms, including for example, plants and yeast. The transformed organisms will then produce polyunsaturated fatty acids. The amino acid sequences are useful for generating enzyme-specific antibodies that are useful for identifying the desaturases.
-
公开(公告)号：US10351871B2
公开(公告)日：2019-07-16
申请号：US15621699
申请日：2017-06-13
摘要： The amino acid and nucleic acid sequences of a Δ5-desaturase enzyme and a Δ8-desaturase enzyme are disclosed. The nucleic acid sequences can be used to design recombinant DNA constructs and vectors. These vectors can then be used to transform various organisms, including for example, plants and yeast. The transformed organisms will then produce polyunsaturated fatty acids. The amino acid sequences are useful for generating enzyme-specific antibodies that are useful for identifying the desaturases.
-
公开(公告)号：US20170283839A1
公开(公告)日：2017-10-05
申请号：US15621699
申请日：2017-06-13
CPC分类号： C12N15/8247 , C12N9/0071 , C12N9/0083 , C12N15/80 , C12N15/8241 , C12N15/85
摘要： The amino acid and nucleic acid sequences of a Δ5-desaturase enzyme and a Δ8-desaturase enzyme are disclosed. The nucleic acid sequences can be used to design recombinant DNA constructs and vectors. These vectors can then be used to transform various organisms, including for example, plants and yeast. The transformed organisms will then produce polyunsaturated fatty acids. The amino acid sequences are useful for generating enzyme-specific antibodies that are useful for identifying the desaturases.
-
6.发明申请COMPOSITIONS AND METHODS FOR DIFFERENTIAL REGULATION OF FATTY ACID UNSATURATION IN MEMBRANE LIPIDS AND SEED OIL 审中-公开膜脂和种子油中脂肪酸不饱和度差异调节的组合物和方法公开(公告)号：US20160272985A1
公开(公告)日：2016-09-22
申请号：US14948166
申请日：2015-11-20
发明人： John A. Browse , Chaofu Lu , Zhanguo Xin
CPC分类号： C12N15/8247 , A01H5/10 , A23D9/013 , C10L1/026 , C10L2200/0476 , C10L2270/026 , C11B1/00 , C12N9/1288 , C12Y207/08002
摘要： Aspects of the invention provide methods for differential regulation of fatty acid unsaturation in seed oil and membrane lipids of plants based on modulation of a previously unknown biosynthetic pathway involving a novel phosphatidylcholine:diacylglycerol cholinephosphotransferase (PDCT) that regulates phosphatidylcholine biosynthesis in developing oil seed plants. Specific aspects relate to inventive PDCT polypeptides including, for example, variants, deletions, muteins, fusion proteins, and orthologs thereof (collectively PDCT proteins), to nucleic acids encoding same, to plants comprising such PDCT sequences or proteins or devoid or depleted of such PDCT proteins or sequences, and to methods for generating plants having altered or no PDCT expression and/or activity, including but not limited to methods comprising mutagenesis, recombinant DNA, transgenics, etc.
摘要翻译： 本发明的方面提供了基于调节以前未知的涉及新型磷脂酰胆碱的生物合成途径的调节脂肪酸不饱和度的方法：在开发油种子植物中调节磷脂酰胆碱生物合成的二酰基甘油胆碱磷酸转移酶（PDCT）。 具体方面涉及本发明的PDCT多肽，包括例如变体，缺失，突变蛋白，融合蛋白及其直系同源物（统称为PDCT蛋白质）与编码该核酸的核酸组合在一起，包含这样的PDCT序列或蛋白质的植物或缺乏或缺失的 PDCT蛋白或序列，以及产生具有改变或不具有PDCT表达和/或活性的植物的方法，包括但不限于包含诱变，重组DNA，转基因等的方法。
-
-
-
-
-
搜索结果
6 条记录 (耗时 0.02 秒)
