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    Proteases from gram-positive organisms
    63.
    发明授权
    Proteases from gram-positive organisms 失效
    来自革兰氏阳性菌的蛋白酶

    公开(公告)号：US08101563B2

    公开(公告)日：2012-01-24

    申请号：US11008352

    申请日：2004-12-08

    申请人： David A. Estell

    发明人： David A. Estell

    IPC分类号： C11D3/386 ,  C12N9/54 ,  C12N9/56 ,  C12N15/57 ,  C12N15/74 ,  C12N15/75

    CPC分类号： C12N9/54 ,  C11D3/386 ,  C12N15/75

    摘要： The present invention relates to the identification of novel metallo-proteases (MP) in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for Bacillus MP. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding MP. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions comprising an MP of the present invention.

    摘要翻译： 本发明涉及革兰阳性微生物中新型金属蛋白酶（MP）的鉴定。 本发明提供了用于芽孢杆菌MP的核酸和氨基酸序列。 本发明还提供具有编码MP的基因的部分或全部的突变或缺失的宿主细胞。 本发明还提供宿主细胞，其进一步包含编码所需异源蛋白质如酶的核酸。 本发明还提供了包含本发明的MP的清洁组合物。

    PRODUCTION OF BUTANOL
    64.
    发明申请
    PRODUCTION OF BUTANOL 审中-公开
    丁醇的生产

    公开(公告)号：US20110097775A1

    公开(公告)日：2011-04-28

    申请号：US12936020

    申请日：2009-04-03

    申请人： Edward Green ,  Elizabeth Jenkinson ,  Mathew Crow

    发明人： Edward Green ,  Elizabeth Jenkinson ,  Mathew Crow

    IPC分类号： C12P7/16 ,  C12N15/74 ,  C12N15/75 ,  C12N1/21

    CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1217 ,  C12N9/88 ,  C12N15/52 ,  C12P7/52 ,  Y02E50/10

    摘要： The present invention relates to methods for making recombinant thermophilic bacteria of the family Bacillaceae. In particular, recombinant thermophilic Bacillaceae are provided which have been engineered to produce butanol and/or butyrate. Preferably, heterologous nucleic acid molecules encoding one or more butanol or butyrate biosynthetic pathway enzymes are introduced into a thermophilic Bacillaceae in order to produce a recombinant thermophilic Bacillaceae which is capable of producing butanol. The Bacillaceae is preferably of the genus Geobacillus or Ureibacillus. The invention also relates to a method of producing butanol using the Bacillaceae of the invention.

    摘要翻译： 本发明涉及用于制备芽孢杆菌科的重组嗜热细菌的方法。 特别地，提供了重组嗜热芽孢杆菌科，其已被工程化以生产丁醇和/或丁酸盐。 优选地，将编码一种或多种丁醇或丁酸盐生物合成途径酶的异源核酸分子引入嗜热芽孢杆菌科，以产生能够生产丁醇的重组嗜热芽胞杆菌科。 芽孢杆菌科优选属地芽孢杆菌属或芽孢杆菌属。 本发明还涉及使用本发明的芽胞杆菌科生产丁醇的方法。

    VARIANTS OF THE BACILLUS LICHENIFORMIS ALPHA-AMYLASE
    65.
    发明申请
    VARIANTS OF THE BACILLUS LICHENIFORMIS ALPHA-AMYLASE 审中-公开
    BACILLUS LICHENIFORMIS ALPHA-AMYLASE的变体

    公开(公告)号：US20110033882A1

    公开(公告)日：2011-02-10

    申请号：US12600818

    申请日：2008-05-28

    申请人： Wolfgang Aehle ,  Neelam S. Amin

    发明人： Wolfgang Aehle ,  Neelam S. Amin

    IPC分类号： C12N9/28 ,  C12N15/56 ,  C12N1/00 ,  C12N15/63 ,  C12N15/75 ,  C12N15/78 ,  C12N15/80 ,  C12N15/76 ,  C12N15/70 ,  C12N1/21 ,  C12N1/15 ,  C11D3/386 ,  D06M16/00 ,  D06L1/14 ,  C12P19/00 ,  A23L1/01 ,  C12Q1/40

    CPC分类号： C12P19/14 ,  A21D8/042 ,  C11D3/386 ,  C12N9/2417 ,  D06M16/003

    摘要： Variants of B. licheniformis alpha-amylase advantageously exhibit improved enzymatic performance. Suitable variants include those with an altered charge distribution on the surface of the enzyme or with altered active site residues. Structural modeling can inform the choice of amino acid modifications so that modified amino acids correspond to residues found in more active alpha amylases, for example. Compositions comprising the variants are useful in methods of cleaning surfaces, laundering textiles, desizing, treating starch, e.g., liquefaction and saccharification, and hydrolyzing biofilms off various substrates.

    摘要翻译： 地衣芽孢杆菌α-淀粉酶的变体有利地表现出改善的酶性能。 合适的变体包括在酶表面具有改变的电荷分布或具有改变的活性位点残基的变体。 结构建模可以指示氨基酸修饰的选择，使得修饰的氨基酸对应于例如在更有活性的α淀粉酶中发现的残基。 包含变体的组合物可用于清洁表面，洗涤纺织品，脱浆，处理淀粉，例如液化和糖化，以及从各种底物上水解生物膜的方法。

    Engineered proteases for affinity purification and processing of fusion proteins
    67.
    发明授权
    Engineered proteases for affinity purification and processing of fusion proteins 有权
    用于融合蛋白亲和纯化和加工的工程蛋白酶

    公开(公告)号：US07824885B2

    公开(公告)日：2010-11-02

    申请号：US10567073

    申请日：2004-06-29

    申请人： Philip N. Bryan

    发明人： Philip N. Bryan

    IPC分类号： C12N15/62 ,  C12N15/70 ,  C12N15/74 ,  C12N15/75 ,  C12N15/79 ,  C12N15/81 ,  C12N15/85 ,  C12P21/06

    CPC分类号： C12N9/54 ,  C12N15/62 ,  C12Q1/37 ,  G01N33/573

    摘要： The present invention is directed to the identification of a protease prodomain that is capable of binding a corresponding protease with high affinity. The protease prodomain of the present invention is fused to a second protein to form a protease prodomain fusion protein. The presence of a protease prodomain protein in a fusion protein allows for easy and selective purification of the second protein by incubation with the corresponding protease.

    摘要翻译： 本发明涉及能够以高亲和力结合相应的蛋白酶的蛋白酶前域的鉴定。 将本发明的蛋白酶原结构域融合到第二蛋白质中以形成蛋白酶前结构域融合蛋白。 融合蛋白中蛋白酶前域蛋白的存在允许通过与相应的蛋白酶孵育来容易地和选择性地纯化第二种蛋白质。

    Alpha-Amylase Mutants
    69.
    发明申请
    Alpha-Amylase Mutants 失效
    α-淀粉酶突变体

    公开(公告)号：US20100144575A1

    公开(公告)日：2010-06-10

    申请号：US12100506

    申请日：2008-04-10

    申请人： Allan Svendsen ,  Henrik Bisgard-Frantzen ,  Torben Vedel Borchert

    发明人： Allan Svendsen ,  Henrik Bisgard-Frantzen ,  Torben Vedel Borchert

    IPC分类号： C12N9/28 ,  G01N33/53 ,  C12Q1/40 ,  C12N9/30 ,  C12N1/21 ,  C12N1/15 ,  C12N15/75 ,  C12N15/80 ,  C11D3/386 ,  C12N15/56

    CPC分类号： C11D3/386 ,  C12N9/2417

    摘要： The present invention relates to a method of constructing a variant of a parent Termamyl-like α-amylase, which variant has α-amylase activity and at least one altered property as compared to the parent α-amylase, comprises i) analysing the structure of the parent Termamyl-like α-amylase to identify at least one amino acid residue or at least one structural part of the Termamyl-like α-amylase structure, which amino acid residue or structural part is believed to be of relevance for altering the property of the parent Termamyl-like α-amylase (as evaluated on the basis of structural or functional considerations), ii) constructing a Termamyl-like α-amylase variant, which as compared to the parent Termamyl-like α-amylase, has been modified in the amino acid residue or structural part identified in i) so as to alter the property, and, optionally, iii) testing the resulting Termamyl-like α-amylase variant with respect to the property in question.

    摘要翻译： 本发明涉及构建母体类戊酰样α-淀粉酶的变体的方法，该变体与母体α-淀粉酶相比具有α-淀粉酶活性和至少一种改变的性质，包括：i）分析 鉴定至少一个氨基酸残基或类戊酰样α-淀粉酶结构的至少一个结构部分，该氨基酸残基或结构部分被认为与改变 母体类戊酰样α-淀粉酶（根据结构或功能考虑进行评估），ii）构建与母体类戊酰样α-淀粉酶相比的类戊酰样α-淀粉酶变体，已经在 在i）中鉴定的氨基酸残基或结构部分，以改变性质，以及任选地，iii）相对于所讨论的性质测试所得到的类戊酰样α-淀粉酶变体。

    Subtilases
    70.
    发明授权
    Subtilases 失效
    分散液

    公开(公告)号：US07727756B2

    公开(公告)日：2010-06-01

    申请号：US10807096

    申请日：2004-03-22

    申请人： Allan Svendsen ,  Stefan Minning

    发明人： Allan Svendsen ,  Stefan Minning

    IPC分类号： C12N9/52 ,  C12N15/57 ,  C12N15/70 ,  C12N15/74 ,  C12N15/75

    CPC分类号： C12N9/54 ,  C07K2299/00

    摘要： The present invention relates to methods for producing variants of a parent JP170 subtilase and of a parent BPN′ subtilase and to JP170 and BPN′ variants having altered properties as compared to the parent JP170/BPN′ subtilase.

    摘要翻译： 本发明涉及与亲本JP170 / BPN'脱羧酶相比，用于产生亲本JP170脱落酶和亲本BPN'脱羧酶的变体的方法和具有改变的性质的JP170和BPN'变体的方法。