公开(公告)号：US20110097775A1

公开(公告)日：2011-04-28

申请号：US12936020

申请日：2009-04-03

申请人： Edward Green ,  Elizabeth Jenkinson ,  Mathew Crow

发明人： Edward Green ,  Elizabeth Jenkinson ,  Mathew Crow

IPC分类号： C12P7/16 ,  C12N15/74 ,  C12N15/75 ,  C12N1/21

CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/1217 ,  C12N9/88 ,  C12N15/52 ,  C12P7/52 ,  Y02E50/10

摘要： The present invention relates to methods for making recombinant thermophilic bacteria of the family Bacillaceae. In particular, recombinant thermophilic Bacillaceae are provided which have been engineered to produce butanol and/or butyrate. Preferably, heterologous nucleic acid molecules encoding one or more butanol or butyrate biosynthetic pathway enzymes are introduced into a thermophilic Bacillaceae in order to produce a recombinant thermophilic Bacillaceae which is capable of producing butanol. The Bacillaceae is preferably of the genus Geobacillus or Ureibacillus. The invention also relates to a method of producing butanol using the Bacillaceae of the invention.

摘要翻译： 本发明涉及用于制备芽孢杆菌科的重组嗜热细菌的方法。 特别地，提供了重组嗜热芽孢杆菌科，其已被工程化以生产丁醇和/或丁酸盐。 优选地，将编码一种或多种丁醇或丁酸盐生物合成途径酶的异源核酸分子引入嗜热芽孢杆菌科，以产生能够生产丁醇的重组嗜热芽胞杆菌科。 芽孢杆菌科优选属地芽孢杆菌属或芽孢杆菌属。 本发明还涉及使用本发明的芽胞杆菌科生产丁醇的方法。