公开(公告)号：US20230036162A1

公开(公告)日：2023-02-02

申请号：US17966281

申请日：2022-10-14

申请人： Academia Sinica

发明人： Ying-Chih CHANG

IPC分类号： C12N5/09 ,  G01N33/50

摘要： The present disclosure provides a substrate for cell culture. Systems comprising the substrate, and methods for using and manufacturing the substrate are also disclosed herein.

公开(公告)号：US20220397526A1

公开(公告)日：2022-12-15

申请号：US17736684

申请日：2022-05-04

申请人： Altius Institute for Biomedical Sciences

发明人： John A. Stamatoyannopoulos ,  Shreeram Akilesh ,  Vivek Nandakumar

IPC分类号： G01N21/64 ,  C12Q1/6841 ,  C40B40/06 ,  G01N21/78 ,  G01N33/574 ,  G01N33/58 ,  G01N33/569 ,  A01N1/02 ,  C12N5/078 ,  C12N5/09 ,  C12P19/34 ,  G01N1/30 ,  C12Q1/6837

摘要： Disclosed herein are methods of detecting a target nucleic acid sequence, determining the localization of the target nucleic acid sequence, and/or quantifying the number of target nucleic acid sequences in a cell. This method may be used on small target nucleic acid sequences, and may be referred to as Nano-FISH.

公开(公告)号：US20220396777A1

公开(公告)日：2022-12-15

申请号：US17770463

申请日：2020-10-26

申请人： Dana-Farber Cancer Institute, Inc. ,  Massachusetts Institute of Technology

发明人： Alexander K. Shalek ,  Peter Winter ,  Andrew Navia ,  Srivatsan Raghavan ,  William Hahn ,  Andrew Aguirre ,  Brian Wolpin ,  Jennyfer Galvez-Reyes

IPC分类号： C12N5/09 ,  G01N33/50 ,  C12Q1/6886

摘要： In certain example embodiments, the invention provides a method of generating an ex vivo cell-based system comprising dissociating an original tissue sample obtained from a subject into a single cell population; determining an in vivo phenotype of the tissue sample by conducting single-cell RNA analysis on a first portion of the single cells; establishing an ex vivo cell-based system from a second portion of the single cells; and culturing the ex vivo cell-based system in a medium or conditions selected to maintain the in vivo phenotype. In some embodiments, the original tissue sample is a tumor tissue sample, such as a pancreatic ductal adenocarcinoma (PDAC) tumor sample.

公开(公告)号：US20220389379A1

公开(公告)日：2022-12-08

申请号：US17775106

申请日：2019-11-18

申请人： PRECEDO PHARMACEUTICALS CO., LTD

发明人： Qingsong LIU ,  Feiyang LIU ,  Husheng MEI ,  Wenchao WANG ,  Ming ZHAO ,  Zongru JIANG ,  Tao REN ,  Li WANG

IPC分类号： C12N5/071 ,  C12N5/09 ,  G01N33/50

摘要： Provided is a culture medium containing amphiregulin for culturing primary breast epithelial cells and a culture method involving using the medium. In the culturing method, primary cells are cultured on a culture vessel coated with an extracellular matrix gel by using the culture medium, and the primary cells grow on the culture vessel, which has been coated with the extracellular matrix gel, and proliferate rapidly under the combined action of nutrient factors and an extracellular matrix contained in the primary cell culture medium. The cell model obtained by the primary cell culture medium and the primary cell culture method can be used for the evaluating the efficacy of and screening drugs.

公开(公告)号：US20220340881A1

公开(公告)日：2022-10-27

申请号：US17632939

申请日：2020-08-07

申请人： UROSPHERE ,  INSTITUTE NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE ,  UNIVERSITE DE TOULOUSE III - PAUL SABATIER ,  ECOLE NATIONALE VETERINAIRE DE TOULOUSE ,  CENTRE HOSPITALIER UNIVERSITAIRE DE TOULOUSE ,  INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT

发明人： PHILIPPE LLUEL ,  NATHALIE VERGNOLLE ,  XAVIER GAME ,  CELINE ROUGET ,  MORGANE SÉBERT

IPC分类号： C12N5/071 ,  C12N5/09

摘要： The subject matter of the present invention is a method for differentiating epithelial stem cells, comprising culturing one or more epithelial stem cells in contact with an extracellular matrix in the presence of an expansion medium, a bovine pituitary extract, a receptor tyrosine kinase ligand, a supernatant of primary fibroblasts and optionally, a Rho kinase inhibitor.

公开(公告)号：US11466056B2

公开(公告)日：2022-10-11

申请号：US16307719

申请日：2017-06-08

申请人： ALMA MATER STUDIORUM UNIVERSITA DI BOLOGNA

发明人： Valerio Leoni ,  Maria Gabriella Campadelli

IPC分类号： C07K14/005 ,  A61P35/00 ,  A61K35/763 ,  A61K45/06 ,  C07K14/39 ,  C07K16/32 ,  C12N5/09 ,  C12N7/00

摘要： The present invention is directed to a recombinant herpesvirus comprising a heterologous peptide and optionally polypeptide ligand capable of binding to (a) target molecule(s) and fused to or inserted into glycoprotein D. The recombinant herpesvirus may additionally comprise modifications for detargeting the virus from the natural receptors of gD. This allows the herpesvirus to efficiently target a cell for therapeutic purposes and a cell for virus production. The present invention further comprises a pharmaceutical composition comprising the herpesvirus, the herpesvirus for use in the treatment of a tumor, infection, degenerative disorder or senescence-associated disease, a nucleic acid and a vector coding for the gD, a polypeptide comprising the gD, and a cell comprising the herpesvirus, nucleic acid, vector or polypeptide. Moreover, a method for infecting a cell with the herpesvirus or for producing the herpesvirus is disclosed.

公开(公告)号：US20220267735A1

公开(公告)日：2022-08-25

申请号：US17630856

申请日：2019-08-05

申请人： GeneX Health Co., Ltd

发明人： Shenyi YIN ,  Hanshuo ZHANG

IPC分类号： C12N5/09 ,  C12N5/071

摘要： A method for culturing primary cells from solid tumor tissues of lung cancer and primary tumor cells from pleural effusion of lung cancer, and the auxiliary reagents. A method for culturing primary cells from solid tumor tissues of lung cancer and primary tumor cells from pleural effusion of lung cancer and the auxiliary reagents. The core of the technology is as follows: (1) solid tumor of lung cancer are treated by using a mild cell dissociating reagent, and lung cancer cells in pleural effusion are dissociated by a mild method, ensuring the vitality of cancer cells to the greatest extent; (2) a special serum-free medium is prepared, and tumor cells derived from solid tumor tissues of lung cancer are cultured in vitro by using a suspension culture system, ensuring the normal amplification of the cancer cells while eliminating the interference of normal cells to the greatest extent.

公开(公告)号：US11414691B2

公开(公告)日：2022-08-16

申请号：US16330722

申请日：2017-09-07

申请人： National University Corporation Kanazawa University

发明人： Masanobu Oshima ,  Mizuho Nakayama ,  Eri Sakai

IPC分类号： C12Q1/02 ,  C12N5/09 ,  C12N5/071 ,  G01N33/50 ,  C12N5/00

摘要： Provided are an organoid having a metastatic property when transplanted into an immunocompetent non-human animal of the same species, a cell strain having a metastatic property when transplanted into an immunocompetent non-human animal of the same species, and a non-human animal including the organoid or the cell strain.

公开(公告)号：US20220249378A1

公开(公告)日：2022-08-11

申请号：US17712732

申请日：2022-04-04

申请人： UPM-KYMMENE CORPORATION

发明人： Heli PAUKKONEN ,  Vili-Veli AUVINEN ,  Marjo YLIPERTTULA ,  Patrick LAURÉN ,  Arto URTTI ,  Timo LAAKSONEN ,  Arto MERIVAARA ,  Tiina HAKKARAINEN ,  Outi MONNI ,  Anne MÄKELÄ ,  Petter SOMERSALO ,  Piia-Riitta KARHEMO ,  Heikki JOENSUU ,  Raili KOIVUNIEMI ,  Kari LUUKKO ,  Markus NUOPPONEN

IPC分类号： A61K9/19 ,  A61K9/06 ,  A61K47/10 ,  A61K47/26 ,  A61K47/38 ,  A01N1/02 ,  C12N5/00 ,  C12N5/09 ,  F26B5/06

摘要： The present disclosure provides a method for freeze-drying cells in a hydrogel comprising nanofibrillar cellulose, the method comprising providing a hydrogel comprising nanofibrillar cellulose, providing cells, combining the cells and the hydrogel comprising nanofibrillar cellulose to form a cell system, and freeze drying the cell system to obtain dried cells in a hydrogel comprising nanofibrillar cellulose. The present disclosure also provides a freeze-dried hydrogel comprising nanofibrillar cellulose and cells.

公开(公告)号：US20220195531A1

公开(公告)日：2022-06-23

申请号：US17441692

申请日：2020-03-23

申请人： APPLIED STEMCELL, INC.

发明人： Vigneshwaran MANI ,  Gianluca ROMA

IPC分类号： C12Q1/6886 ,  C12N15/90 ,  C12N5/09 ,  C12Q1/6806

摘要： Provided herein are methods for producing exosomes that contain RNA transcribed from a specific mutant gene or a transgene. In one embodiment, the method comprises the steps of: generating a cell comprising a mutation of a gene by using a site-specific nuclease; culturing the cell in a medium that allows the cell to secrete to the medium an exosome containing an RNA transcribed from the gene and comprising the mutation; and collecting the medium that contains the exosome. The exosomes generated can be used as reference material or therapeutic delivery device.