公开(公告)号：US10690677B2

公开(公告)日：2020-06-23

申请号：US15487349

申请日：2017-04-13

Applicant: ACEA Biosciences, Inc.

Inventor： Xiao Xu ,  Yama A. Abassi ,  Xiaobo Wang ,  Jiangbo Gan

IPC: G01N33/68 ,  C12M1/34 ,  C12M1/12 ,  G01N33/50 ,  G01N33/483

Abstract: Methods for identifying compounds that modulate cellular responses stimulated by IgE, which include providing an impedance-based system that monitors cell-substrate impedance of cells on a substrate; introducing cells to the substrate of the system; adding at least one test compound and IgE to the cells, wherein the at least one test compound is suspected of modulating cell responses stimulated by the IgE; adding an antigen to the cells; monitoring the cell-substrate impedance of cells on the substrate; and analyzing the cell-substrate impedance to evaluate whether the at least one test compound alters a cellular response to stimulation with the IgE.

公开(公告)号：US10539523B2

公开(公告)日：2020-01-21

申请号：US14092927

申请日：2013-11-28

Applicant: ACEA Biosciences, Inc.

Inventor： Xiaobo Wang ,  Wei Ouyang ,  Nan Li ,  Tianxing Wang ,  Xiaoyu Zhang ,  Xiao Xu ,  Yama A. Abassi

IPC: G01N27/02

Abstract: Devices, systems and methods for monitoring excitable cells, such as cardiomyocytes, on microelectrode arrays that couple the electro-stimulation of excitable cells to induce or regulate cardiomyocyte beating and the simultaneous measurement of impedance and extracellular recording to assess changes in cardiomyocyte beating, viability, morphology or electrophysical properties in response to a plurality of treatments.

公开(公告)号：US10533985B2

公开(公告)日：2020-01-14

申请号：US16020679

申请日：2018-06-27

Applicant: ACEA Biosciences, Inc.

Inventor： Xiaobo Wang ,  Yama A. Abassi ,  Biao Xi ,  Wen Fu Zhang ,  Xiao Xu

IPC: G01N33/483 ,  G01N33/487

Abstract: A system for monitoring cell-substrate impedance of excitable cells and monitoring the beating cycle of cardiomyocytes, the system including: a device for monitoring cell-substrate impedance at 20 millisecond time resolution, the device having one or more wells on a nonconductive substrate, wherein at least one well of the one or more wells comprises an electrode array fabricated on the substrate for measurement of cell-substrate impedance at 20 millisecond time resolution; an impedance analyzer that measures cell-substrate impedance from the at least one well at 20 millisecond time resolution; electronic circuitry that electrically connects the electrode array from the at least one well to the impedance analyzer; and a software program that analyzes the measured cell-substrate impedance.

公开(公告)号：US20190195861A1

公开(公告)日：2019-06-27

申请号：US16237382

申请日：2018-12-31

Applicant: ACEA Biosciences, Inc.

Inventor： Yama A. Abassi ,  Naichen Yu ,  Josephine Atienza ,  Xiao Xu ,  Xiaobo Wang

IPC: G01N33/50 ,  G01N27/02 ,  G01N33/543 ,  C12Q1/00

CPC classification number: G01N33/5041 ,  C12Q1/002 ,  G01N27/021 ,  G01N33/5014 ,  G01N33/5438 ,  G01N2333/4716 ,  G01N2333/705 ,  G01N2333/91205 ,  G01N2500/04 ,  G01N2500/10

Abstract: A method of identifying a therapeutic compound for treating cancer in a human subject, the method including: providing a device that measures cell-substrate impedance; culturing cancer cells in the at least two wells, wherein the cancer cells are obtained from a human subject and have a receptor tyrosine kinase (RTK) pathway; adding to a first well a proposed therapeutic compound that affects an RTK pathway and an RTK stimulating factor for the RTK pathway to form a test well, and adding to another well the RTK stimulating factor to form a control well; continuously monitoring cell-substrate impedance of the at least two wells; and determining a difference in impedance or optionally in cell index between the test well and control well; and if significantly different, concluding the proposed therapeutic compound is therapeutically active in the RTK pathway within the cancer cells of the human subject.

公开(公告)号：US09869628B2

公开(公告)日：2018-01-16

申请号：US14750613

申请日：2015-06-25

Applicant: ACEA Biosciences, Inc.

Inventor： Nan Li ,  Sean Lee ,  Kilo Vilayphone ,  Xiaobo Wang

IPC: G01N15/14 ,  G01N15/10

CPC classification number: G01N15/1404 ,  G01N2015/1006 ,  G01N2015/1486

Abstract: A method of collecting cells from individual wells of a multi-well plate for use in flow cytometry, the method including adding a suspension of cells to wells of the multi-well plate; and aspirating cells from different wells according to a collection pattern into a flow cytometer, wherein the collection pattern is a sequential ordering of wells beginning at a middle region of the multi-well plate and continuing towards an outer region of the multi-well plate. The method preferably including rotating or agitating the multi-well plate between steps of aspirating cells from different wells. Exemplary collection patterns include spiral-square collection pattern and a nearest well to center collection pattern.

公开(公告)号：US20170205391A1

公开(公告)日：2017-07-20

申请号：US15478041

申请日：2017-04-03

Applicant: ACEA Biosciences, Inc.

Inventor： Nan Li ,  Xiaobo Wang ,  Yama A. Abassi ,  Biao Xi ,  Wen Fu Zhang ,  Xiao Xu

IPC: G01N33/487 ,  G01N33/50

CPC classification number: G01N33/48728 ,  G01N33/4836 ,  G01N33/5008 ,  G01N33/5014 ,  G01N33/5061 ,  G01N33/5073 ,  G01N2800/326 ,  G01N2800/52

Abstract: A method of determining a beating parameter of cells that undergo excitation contraction coupling, the method including providing a cell analysis device having a substrate and a sensor that measures cell adhesion or attachment to the substrate in millisecond time resolution; adding excitable cells capable of undergoing excitation contraction coupling to the substrate; monitoring cell adhesion or attachment of the excitable cells to the substrate in millisecond time resolution; and calculating one or more beating parameters from the monitored adhesion.

公开(公告)号：US09575063B2

公开(公告)日：2017-02-21

申请号：US14547963

申请日：2014-11-19

Applicant: ACEA Biosciences, Inc.

Inventor： Nan Li ,  Ye Chen ,  Hyunsun Chung ,  Zengqiang Li ,  Xiaobo Wang

IPC: G01N21/00 ,  G01N33/569 ,  G01N15/14 ,  G01N15/10

CPC classification number: G01N21/645 ,  G01N15/1434 ,  G01N15/1459 ,  G01N21/6486 ,  G01N33/56972 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1438 ,  G01N2015/1477 ,  G01N2021/6471 ,  G01N2021/6478 ,  G01N2201/06113

Abstract: An optical engine for use in a bench top flow cytometer, the optical engine comprising a set of lasers; a different set of beam shaping optics for each laser, wherein each set comprises two lenses to adjustably focus light horizontally along an x-axis to a same horizontal position and vertically along a y-axis to a different vertical position along a same plane; collection optics for collecting fluorescence from the flow cell; filtration optics that filter the collected fluorescence from the flow cell into different detection channels according to wavelength ranges; and a detector for each detection channel that converts the filtered fluorescence to electrical signals, wherein electrical signals are processed so that the fluorescence from each laser at the different vertical positions is distinguished at the same detector.

Abstract translation: 一种用于台式流式细胞仪的光学引擎，该光学引擎包括一组激光器; 用于每个激光器的不同组的光束整形光学器件，其中每个组件包括两个透镜，用于可调节地将光沿x轴水平地聚焦到相同的水平位置，并且沿着y轴沿着同一平面垂直地垂直于不同的垂直位置; 用于从流动池收集荧光的收集光学器件; 过滤光学器件，根据波长范围将从流通池收集的荧光过滤到不同的检测通道; 以及用于将滤波后的荧光转换为电信号的每个检测通道的检测器，其中对电信号进行处理，使得来自不同垂直位置处的每个激光的荧光在相同的检测器处被区分。

公开(公告)号：US09464089B2

公开(公告)日：2016-10-11

申请号：US14329890

申请日：2014-07-11

Applicant: ACEA BIOSCIENCES INC.

Inventor： Xiao Xu ,  Xiaobo Wang ,  Long Mao ,  Li Zhao ,  Biao Xi

IPC: A61K31/506 ,  C07D241/04 ,  C07D239/30 ,  C07D487/04 ,  C07D239/52 ,  C07D239/49 ,  C07D403/12 ,  C07D401/12 ,  C07D473/18 ,  C07D417/12

CPC classification number: C07D519/00 ,  C07D239/47 ,  C07D239/48 ,  C07D239/49 ,  C07D239/52 ,  C07D401/12 ,  C07D403/12 ,  C07D417/12 ,  C07D473/18 ,  C07D487/04

Abstract: The present invention relates to pharmaceutical compounds, compositions and methods, especially as they are related to compositions and methods for the treatment and/or prevention of a proliferation disorder, a cancer, a tumor, an inflammatory disease, an autoimmune disease, psoriasis, dry eye or an immunologically related disease, and in some embodiments diseases or disorders related to the dysregulation of kinase such as, but not limited to, EGFR (including HER), Alk, PDGFR, BLK, BMX/ETK, FLT3(D835Y), ITK, TEC, TXK, BTK, or JAK, and the respective pathways.

Abstract translation: 本发明涉及药物化合物，组合物和方法，特别是因为它们与用于治疗和/或预防增殖病症，癌症，肿瘤，炎性疾病，自身免疫性疾病，牛皮癣，干性疾病的组合物和方法有关 眼睛或免疫相关疾病，在一些实施方案中，与激酶失调相关的疾病或病症，例如但不限于EGFR（包括HER），Alk，PDGFR，BLK，BMX / ETK，FLT3（D835Y），ITK ，TEC，TXK，BTK或JAK，以及各自的途径。

公开(公告)号：US20160097707A1

公开(公告)日：2016-04-07

申请号：US14750613

申请日：2015-06-25

Applicant: ACEA Biosciences, Inc.

Inventor： Nan Li ,  Sean Lee ,  Kilo Vilayphone ,  Xiaobo Wang

IPC: G01N15/14

CPC classification number: G01N15/1404 ,  G01N2015/1006 ,  G01N2015/1486

Abstract: A method of collecting cells from individual wells of a multi-well plate for use in flow cytometry, the method including adding a suspension of cells to wells of the multi-well plate; and aspirating cells from different wells according to a collection pattern into a flow cytometer, wherein the collection pattern is a sequential ordering of wells beginning at a middle region of the multi-well plate and continuing towards an outer region of the multi-well plate. The method preferably including rotating or agitating the multi-well plate between steps of aspirating cells from different wells. Exemplary collection patterns include spiral-square collection pattern and a nearest well to center collection pattern.

Abstract translation: 一种从多孔板的单个孔中收集细胞用于流式细胞术的方法，所述方法包括向多孔板的孔中加入细胞悬浮液; 以及根据收集模式将不同孔中的细胞抽吸到流式细胞仪中，其中所述收集模式是从所述多孔板的中间区域开始并且朝向所述多孔板的外部区域继续的孔的连续排序。 该方法优选地包括在从不同孔抽吸细胞的步骤之间旋转或搅拌多孔板。 示例性收集模式包括螺旋形收集模式和最近的井到中心收集模式。

公开(公告)号：US09158118B2

公开(公告)日：2015-10-13

申请号：US13656457

申请日：2012-10-19

Applicant: ACEA BIOSCIENCES, INC

Inventor： Nan Li ,  Ye Chen ,  Xiaobo Wang

IPC: G02B27/10 ,  G01J3/443 ,  G02B5/04 ,  G02B27/14 ,  G01J3/14 ,  G01J3/36 ,  G02B5/28

CPC classification number: G02B27/1006 ,  G01J3/14 ,  G01J3/36 ,  G01J3/443 ,  G02B5/04 ,  G02B5/285 ,  G02B27/141 ,  G02B27/142

Abstract: A prism including a substrate faceted to provide a plurality of flat surfaces, wherein at least two of the plurality of surfaces, each including a filter coating, form at least two filter surfaces, wherein each filter surface selectively permits passage of a predetermined wavelength and reflects remaining wavelengths along an optical path towards another of the plurality of surfaces, optionally another filter surface, wherein an angle of incidence of each of the plurality of surfaces along the optical path is equal or nearly equal. An apparatus incorporating the prism and its use for splitting a light spectrum into a plurality of wavelengths or wavelength ranges.

Abstract translation: 一种棱镜，包括面以提供多个平坦表面的基板，其中，所述多个表面中的至少两个表面，每个包括过滤器涂层，形成至少两个过滤器表面，其中每个过滤器表面选择性地允许预定波长的通过并反射 沿着光路的剩余波长朝向多个表面中的另一个，可选地另外的滤光器表面，其中沿着光路的多个表面中的每一个的入射角相等或接近相等。 一种结合棱镜的装置及其用于将光谱分解成多个波长或波长范围的用途。