公开(公告)号：US20180230430A1

公开(公告)日：2018-08-16

申请号：US15946101

申请日：2018-04-05

Applicant: Wisconsin Alumni Research Foundation

Inventor： Igor I. Slukvin ,  James A. Thomson ,  Maksym A. Vodyanyk ,  Maryna E. Gumenyuk

IPC: C12N5/078

CPC classification number: C12N5/0641 ,  C12N2500/25 ,  C12N2501/125 ,  C12N2501/14 ,  C12N2501/145 ,  C12N2501/23 ,  C12N2501/39 ,  C12N2502/1394 ,  C12N2506/02

Abstract: Methods and compositions of erythroid cells that produce adult β-hemoglobin, generated by culturing CD31+, CD31+/CD34+ or CD34+ cells from embryonic stem cells under serum-free culture conditions.

公开(公告)号：US20160244719A1

公开(公告)日：2016-08-25

申请号：US15048789

申请日：2016-02-19

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson ,  Jue Zhang

IPC: C12N5/071 ,  G01N33/50 ,  A61K35/44

CPC classification number: C12N5/069 ,  A61K35/44 ,  C12N5/0031 ,  C12N5/0056 ,  C12N2500/35 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2501/10 ,  C12N2501/113 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/165 ,  C12N2501/415 ,  C12N2501/42 ,  C12N2506/02 ,  C12N2506/45 ,  G01N33/5064

Abstract: Methods for generating human arterial endothelial cells under defined conditions in the absence of insulin are described.

Abstract translation: 描述了在不存在胰岛素的条件下产生人动脉内皮细胞的方法。

公开(公告)号：US20160194611A1

公开(公告)日：2016-07-07

申请号：US15063046

申请日：2016-03-07

Applicant: Wisconsin Alumni Research Foundation

Inventor： Guokai Chen ,  James A. Thomson

IPC: C12N5/074

CPC classification number: C12N5/0696 ,  C12N5/0606 ,  C12N2500/02 ,  C12N2500/32 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2501/105 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/15 ,  C12N2501/33 ,  C12N2501/727 ,  C12N2501/845 ,  C12N2501/998

Abstract: Fully defined media that support pluripotent cell viability, proliferation, cloning, and derivation, as well as methods and compositions including these media are described. Methods for deriving iPS cells from adult individuals under defined, xeno-free conditions are also described.

Abstract translation: 描述了支持多能细胞活力，增殖，克隆和衍生的完全限定的培养基，以及包括这些培养基的方法和组合物。 还描述了在确定的无异种条件下从成年个体获得iPS细胞的方法。

公开(公告)号：US20150056698A1

公开(公告)日：2015-02-26

申请号：US14281341

申请日：2014-05-19

Applicant: Wisconsin Alumni Research Foundation

Inventor： James A. Thomson

IPC: C12N5/073

CPC classification number: C12N5/0605 ,  C12N5/0603 ,  C12N5/0606 ,  C12N2502/13 ,  C12N2506/02

Abstract: A purified preparation of primate embryonic stem cells is disclosed. This preparation is characterized by the following cell surface markers: SSEA-1 (−); SSEA-4 (+); TRA-1-60 (+); TRA-1-81 (+); and alkaline phosphatase (+). In a particularly advantageous embodiment, the cells of the preparation are human embryonic stem cells, have normal karyotypes, and continue to proliferate in an undifferentiated state after continuous culture for eleven months. The embryonic stem cell lines also retain the ability, throughout the culture, to form trophoblast and to differentiate into all tissues derived from all three embryonic germ layers (endoderm, mesoderm and ectoderm). A method for isolating a primate embryonic stem cell line is also disclosed.

Abstract translation: 公开了灵长类动物胚胎干细胞的纯化制剂。 该制剂的特征在于以下细胞表面标志物：SSEA-1（ - ）; SSEA-4（+）; TRA-1-60（+）; TRA-1-81（+）; 和碱性磷酸酶（+）。 在一个特别有利的实施方案中，制剂的细胞是人胚胎干细胞，具有正常的核型，并且在连续培养11个月后，以未分化状态继续增殖。 胚胎干细胞系还保留了整个培养物中形成滋养细胞并分化成来自所有三个胚胎胚层（内胚层，中胚层和外胚层）的所有组织的能力。 还公开了分离灵长类动物胚胎干细胞系的方法。

公开(公告)号：US20130230917A1

公开(公告)日：2013-09-05

申请号：US13859228

申请日：2013-04-09

Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION

Inventor： Igor I. Slukvin ,  James A. Thomson ,  Maksym A. Vodyanyk ,  Maryna E. Gumenyuk

IPC: C12N5/0784

CPC classification number: C12N5/0639 ,  C12N5/064 ,  C12N2506/02

Abstract: This invention relates to the culture of dendritic cells from human embryonic stem (ES) cells. Human ES cells are first cultured into hematopoietic cells by co-culture with stromal cells. The cells now differentiated into the hematopoietic lineage are then cultured with GM-CSF to create a culture of myeloid precursor cells. Culture of the myeloid precursor cells with the cytokines GM-CSF and IL-4 causes functional dendritic cells to be generated. The dendritic cells have a unique phenotype, as indicated by their combination of cell surface markers.

Abstract translation: 本发明涉及来自人胚胎干（ES）细胞的树突状细胞的培养。 首先通过与基质细胞共培养将人类ES细胞培养成造血细胞。 然后将分化成造血谱系的细胞与GM-CSF一起培养以产生骨髓前体细胞的培养物。 用细胞因子GM-CSF和IL-4培养骨髓前体细胞会导致产生功能性树突状细胞。 树突细胞具有独特的表型，如细胞表面标志物的组合所示。