公开(公告)号：US20230164895A1

公开(公告)日：2023-05-25

申请号：US17877630

申请日：2022-07-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Edward George MARQUETTE

IPC: H05B45/46

CPC classification number: H05B45/46

Abstract: A circuit for sharing current between parallel LEDs or parallel strings of LEDs, and a method of use of the same, are disclosed herein. The circuit for sharing current between parallel LED pathways can include a first LED pathway, a first transistor coupled to the first set of LEDs and that can control a first current through the first set of LEDs, and a first measurement node having a first sensed voltage. The circuit can include a second LED pathway, a second transistor coupled to the second set of LEDs and that can control a second current through the second set of LEDs, and a second measurement node having a second sensed voltage. The circuit includes a first differential amplifier and a second differential amplifier, each of which can compare sensed voltage and can apply a voltage to a gate of one of the first and second transistors.

公开(公告)号：US11633739B2

公开(公告)日：2023-04-25

申请号：US17137274

申请日：2020-12-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kevin D. Ness ,  Donald A. Masquelier ,  Billy W. Colston, Jr. ,  Benjamin J. Hindson

IPC: B01L3/00 ,  B01L3/02 ,  B01L7/00 ,  G01N21/3563 ,  B01F23/41 ,  B01F33/3011 ,  B01F35/10 ,  C12Q1/686 ,  G01N21/64 ,  B29C45/00 ,  G01N21/49 ,  B01F101/23 ,  B29L31/00

Abstract: System for performing a flow-based assay. The system may comprise a droplet generator to produce an emulsion including droplets in a carrier fluid. The system also may comprise a thermocycler including two or more temperature-controlled zones and also including a channel connected to the droplet generator for receiving the emulsion. The channel may form a single-pass continuous fluid route traversing the temperature-controlled zones multiple times, such that droplets passing through the channel are thermally cycled. The system further may comprise a detection station downstream from the thermocycler and configured to detect a signal from the droplets after such droplets have been thermally cycled by passing through the channel.

公开(公告)号：US20230086701A1

公开(公告)日：2023-03-23

申请号：US17946555

申请日：2022-09-16

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Evan THRUSH ,  Stephen SWIHART ,  Kevin McDONALD

IPC: G01N21/64 ,  G06T5/50 ,  G06V10/60 ,  G01N21/76 ,  G01N33/68

Abstract: Aspects of the present disclosure relate to systems and methods for generating a composite image. This can include a western blot imager with a real time camera. One aspect of the present disclosure relates to an imaging system. The imaging system includes a sample plane that can receive and hold a sample, a photon resolving camera, and a lens attached to the photon resolving camera, the photon resolving camera and the lens positioned to image the sample plane.

公开(公告)号：US20230070234A1

公开(公告)日：2023-03-09

申请号：US17986873

申请日：2022-11-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： David P. STUMBO ,  George CARMAN ,  Steve HOBBS ,  Anthony J. MAKAREWICZ, JR. ,  Dmitri SIMONIAN ,  David GLADE ,  Joshua OEN ,  Denis PRISTINSKI ,  John DZENITIS

IPC: C12Q1/686 ,  B01L3/00 ,  G01N15/14 ,  C12Q1/6848 ,  C12Q1/6844 ,  C12Q1/6851 ,  B01F23/41

Abstract: Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.

公开(公告)号：US11590486B2

公开(公告)日：2023-02-28

申请号：US17008212

申请日：2020-08-31

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jiali Liao ,  Lee Olech ,  Hong Chen ,  Xuemei He ,  Russell Frost

IPC: B01J39/26 ,  C07K16/06 ,  C07K1/16 ,  C07K1/22

Abstract: A method for manufacturing a mixed-mode chromatography medium is provided. The method can include, for example, oxidizing diol groups on diol-functionalized solid particles having pores of a median diameter of 0.5 micron or greater with substantially no pores of 0.1 micron or less in diameter and having a diol density of from about 200 to about 300 μmol/mL to aldehyde groups, thereby converting said diol-functionalized solid particles to aldehyde-functionalized solid particles; and coupling amine-functionalized ligands to said aldehyde-functionalized solid particles, said amine-functionalized ligands comprising an amine-substituted hydrophobic group joined to an acid moiety selected from the group consisting of a carboxyl group and a sulfo group.

公开(公告)号：US20230057343A1

公开(公告)日：2023-02-23

申请号：US17855199

申请日：2022-06-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Duc DO ,  Claudia LITTERST ,  Dianna MAAR

IPC: C12Q1/686 ,  C12Q1/6844 ,  G01N21/64

Abstract: Digital assay system, including methods, apparatus, and compositions, for assay of one or more targets in a set of partitions containing a generic reporter of target amplification.

公开(公告)号：US11579155B2

公开(公告)日：2023-02-14

申请号：US16228597

申请日：2018-12-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John Christopher Yundt-Pacheco ,  Lakshmi Kuchipudi ,  Curtis Alan Parvin

IPC: G16H50/30 ,  G16H10/40 ,  G16H20/10 ,  G01N35/00 ,  G16Z99/00

Abstract: Methods, apparatuses, and systems are disclosed for analyzing quality control (QC) strategies that are applied to testing processes an analyte in order to meet an acceptable level of probability of patient harm that could result from incorrect test results. The measure of patient harm takes into account severity of patient harm, as well as its occurrence. Methods include calculating, based on the parameters of the QC strategies and the test apparatus, an expected number of incorrect final results E(Nuf) due to a test system failure. The value of E(Nuf) can be used as part of a calculation of a predicted level of probability patient harm. The ratio of the acceptable level of probability of patient harm to the predicted level of probability patient harm can determine the adequacy of the QC strategies.

公开(公告)号：US11578322B2

公开(公告)日：2023-02-14

申请号：US16867256

申请日：2020-05-05

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kalyan Handique ,  Austin Payne ,  Sida Wang ,  Patrick Michael Tegels ,  William T. Koederitz ,  Daniel Genord ,  Grey Parker ,  Brian Boniface ,  Katlyn Curtin Mehne ,  Alec William Hitchiner

IPC: C12Q1/68 ,  C12N15/10 ,  B01L3/00 ,  B01L7/00 ,  G01F23/292 ,  C12N1/06 ,  B01L9/02 ,  G01N35/00 ,  C12Q1/6844

Abstract: A system and method for automated single cell capture and processing is described, where the system includes a deck supporting and positioning a set of sample processing elements; a gantry for actuating tools for interactions with the set of sample processing elements supported by the deck; and a base supporting various processing subsystems and a control subsystems in communication with the processing subsystems. The system can automatically execute workflows associated with single cell processing, including mRNA capture, cDNA synthesis, protein-associated assays, and library preparation, for next generation sequencing.

公开(公告)号：US20230043703A1

公开(公告)日：2023-02-09

申请号：US17813942

申请日：2022-07-21

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： YAN WANG ,  STEVEN TAKASHI OKINO ,  JASON MA

IPC: C12Q1/6827 ,  C12Q1/6883 ,  C12Q1/686

Abstract: The subject invention pertains to the detection and differentiation of genetic variations by nucleic acid amplification. The invention provides methods of detecting one or more genetic variations in a nucleic acid that are in close proximity simultaneously. The invention further provides primer and probe oligonucleotides and methods of using said primers and probes in assays to detect genetic variants of concern of SARS-CoV-2. The methods of the invention detect genetic variants of other pathogens, including influenza, or genetic variants involved in inheritable diseases or cancer.

公开(公告)号：US20230031105A1

公开(公告)日：2023-02-02

申请号：US17962360

申请日：2022-10-07

Applicant: Bio-Rad Laboratories, Inc.

Inventor： James Barry ,  Vishal Mehra ,  Alireza Ebrahim

IPC: B01L3/00 ,  G01N35/10

Abstract: A reagent sleeve system such as can be used with various analyzers using chemical phenomena, analyzers and methods of using the reagent sleeve system and analyzer are described. The reagent sleeve system can include a reagent sleeve; and a plurality of removable reagent reservoirs located within the reagent sleeve, wherein each of the plurality of removable reagent reservoirs comprises a respective peripheral wall, and first and second end walls, whereby each of the plurality of removable reagent reservoirs contains a reagent located therein, and the first end wall is pierceable by a reagent extractor, and the reagent sleeve comprises a peripheral wall that encompasses the plurality of removable reagent reservoirs and holds the plurality of reagent reservoirs for sequential use.