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    DIGITAL AMPLIFICATION ASSAYS FOR GENETIC INSTABILITY
    2.
    发明申请
    DIGITAL AMPLIFICATION ASSAYS FOR GENETIC INSTABILITY 有权

    公开(公告)号：US20210388429A1

    公开(公告)日：2021-12-16

    申请号：US17461914

    申请日：2021-08-30

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Dianna MAAR

    IPC: C12Q1/6848 ,  C12Q1/6832 ,  C12Q1/6858

    Abstract: Methods and compositions for detecting genetic instability using digital amplification assays. The methods may be performed in a set of isolated volumes and generally may involve competitive hybridization of a competitor and a probe/primer with a normal allele and one or more mutant alleles of a microsatellite locus. The competitor may be configured to compete similarly with, or to outcompete, the primer/probe for hybridization with the normal allele. The primer/probe may be configured to outcompete the competitor for hybridization with various mutant alleles of the locus that alter the length of the repetitive sequence by different amounts. Isolated volumes in which the primer/probe outcompetes the competitor may be enumerated, and represent one or more of the mutant alleles. The methods may enable diagnosing microsatellite instability and treating a subject based on the diagnosis.

    DIGITAL AMPLIFICATION ASSAYS WITH UNCONVENTIONAL AND/OR INVERSE CHANGES IN PHOTOLUMINESCENCE
    4.
    发明申请
    DIGITAL AMPLIFICATION ASSAYS WITH UNCONVENTIONAL AND/OR INVERSE CHANGES IN PHOTOLUMINESCENCE 审中-公开

    公开(公告)号：US20190119736A1

    公开(公告)日：2019-04-25

    申请号：US16159488

    申请日：2018-10-12

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Eli A. HEFNER ,  Dianna MAAR

    IPC: C12Q1/6844 ,  G01N21/64

    Abstract: Methods of, and compositions for, analysis using digital amplification assays with unconventional/inverse changes in photoluminescence to indicate the presence of one or more targets. In an exemplary method, isolated volumes may be formed, only a subset of which contain a target. Each volume may include a probe having a label, a sink having a quencher and configured to hybridize with the probe to quench the label, and a separator configured to hybridize with the probe and/or the sink to block hybridization of the sink with the probe. An amplification reaction may be performed in the volumes to generate an amplicon corresponding to the target. The separator may hybridize with the amplicon, and may be extended or degraded by the amplification reaction. Photoluminescence of the label may be detected from the volumes, and the photoluminescence of target-positive volumes may be less than that of target-negative volumes.

    AMPLIFICATION REPORTER WITH BASE-PAIRING OLIGOMERS
    6.
    发明申请
    AMPLIFICATION REPORTER WITH BASE-PAIRING OLIGOMERS 有权

    公开(公告)号：US20220033883A1

    公开(公告)日：2022-02-03

    申请号：US17396148

    申请日：2021-08-06

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： John F. REGAN ,  Dianna MAAR ,  Dawne N. SHELTON ,  Tina C. LEGLER ,  Samantha COOPER ,  Wei YANG ,  Eli A. HEFNER ,  Niels KLITGORD

    IPC: C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6848

    Abstract: Method of analysis for alleles of a target. In the method, a plurality of fluid volumes may be formed. Each fluid volume may contain a first primer pair to amplify a first allele of a target, a second primer pair to amplify a second allele of the target, a first reporter including a first photoluminophore and providing a primer of the first primer pair, and a second reporter including a second photoluminophore and providing a primer of the second primer pair. Each of the first and second reporters may include an oligomer having a quencher, and the oligomer may be configured to base-pair with the primer of the first primer pair and the primer of the second primer pair. The first and second alleles may be amplified using the first and second primer pairs. Photoluminescence may be detected. A level of each allele may be determined.

    AMPLIFICATION REPORTER WITH BASE-PAIRING OLIGOMERS
    7.
    发明申请
    AMPLIFICATION REPORTER WITH BASE-PAIRING OLIGOMERS 审中-公开

    公开(公告)号：US20200270677A1

    公开(公告)日：2020-08-27

    申请号：US16834854

    申请日：2020-03-30

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： John F. REGAN ,  Dianna MAAR ,  Dawne N. SHELTON ,  Tina C. LEGLER ,  Samantha COOPER ,  Wei YANG ,  Eli A. HEFNER ,  Niels KLITGORD

    IPC: C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6848

    Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.

    AMPLIFICATION REPORTER WITH BASE-PAIRING OLIGOMERS
    8.
    发明申请
    AMPLIFICATION REPORTER WITH BASE-PAIRING OLIGOMERS 有权

    公开(公告)号：US20250011842A1

    公开(公告)日：2025-01-09

    申请号：US18761103

    申请日：2024-07-01

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： John F. REGAN ,  Dianna MAAR ,  Dawne N. SHELTON ,  Tina C. LEGLER ,  Samantha COOPER ,  Wei YANG ,  Eli A. HEFNER ,  Niels KLITGORD

    IPC: C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6848

    Abstract: A method of analysis for alleles of a target includes forming plural fluid volumes. Each of those volumes includes first and second primer pairs to amplify corresponding first and second alleles of a target. Each of those volumes also includes first and second reporters with corresponding first and second photoluminophores and providing corresponding primers of the corresponding first and second primer pairs. Each of the first and second reporters may include an oligomer that has a quencher and is configured to base-pair with the primer of the corresponding first and second primer pair. The first and second alleles may be amplified using the corresponding first and second primer pairs. The method detects photoluminescence and determines a level of each allele.

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