公开(公告)号：US20250011842A1

公开(公告)日：2025-01-09

申请号：US18761103

申请日：2024-07-01

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John F. REGAN ,  Dianna MAAR ,  Dawne N. SHELTON ,  Tina C. LEGLER ,  Samantha COOPER ,  Wei YANG ,  Eli A. HEFNER ,  Niels KLITGORD

IPC: C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6848

Abstract: A method of analysis for alleles of a target includes forming plural fluid volumes. Each of those volumes includes first and second primer pairs to amplify corresponding first and second alleles of a target. Each of those volumes also includes first and second reporters with corresponding first and second photoluminophores and providing corresponding primers of the corresponding first and second primer pairs. Each of the first and second reporters may include an oligomer that has a quencher and is configured to base-pair with the primer of the corresponding first and second primer pair. The first and second alleles may be amplified using the corresponding first and second primer pairs. The method detects photoluminescence and determines a level of each allele.

公开(公告)号：US20220033883A1

公开(公告)日：2022-02-03

申请号：US17396148

申请日：2021-08-06

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John F. REGAN ,  Dianna MAAR ,  Dawne N. SHELTON ,  Tina C. LEGLER ,  Samantha COOPER ,  Wei YANG ,  Eli A. HEFNER ,  Niels KLITGORD

IPC: C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6848

Abstract: Method of analysis for alleles of a target. In the method, a plurality of fluid volumes may be formed. Each fluid volume may contain a first primer pair to amplify a first allele of a target, a second primer pair to amplify a second allele of the target, a first reporter including a first photoluminophore and providing a primer of the first primer pair, and a second reporter including a second photoluminophore and providing a primer of the second primer pair. Each of the first and second reporters may include an oligomer having a quencher, and the oligomer may be configured to base-pair with the primer of the first primer pair and the primer of the second primer pair. The first and second alleles may be amplified using the first and second primer pairs. Photoluminescence may be detected. A level of each allele may be determined.

公开(公告)号：US20200270677A1

公开(公告)日：2020-08-27

申请号：US16834854

申请日：2020-03-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John F. REGAN ,  Dianna MAAR ,  Dawne N. SHELTON ,  Tina C. LEGLER ,  Samantha COOPER ,  Wei YANG ,  Eli A. HEFNER ,  Niels KLITGORD

IPC: C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6848

Abstract: System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.