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39 条记录 (耗时 0.027 秒)

    Method for accumulating protein in plant cells
    32.
    发明授权
    Method for accumulating protein in plant cells 有权
    在植物细胞中积累蛋白质的方法

    公开(公告)号：US09447396B2

    公开(公告)日：2016-09-20

    申请号：US14003278

    申请日：2012-02-24

    申请人： Yoichi Ogawa ,  Yasuhiro Kondo ,  Ikuko Nishimura ,  Makoto Shirakawa ,  Haruko Ueda ,  Tomoo Shimada

    发明人： Yoichi Ogawa ,  Yasuhiro Kondo ,  Ikuko Nishimura ,  Makoto Shirakawa ,  Haruko Ueda ,  Tomoo Shimada

    IPC分类号： C12N15/82 ,  C12N15/56 ,  C07K14/415 ,  C12N9/24 ,  C12N9/26

    CPC分类号： C12N9/2405 ,  C07K2319/01 ,  C12N9/2408 ,  C12N15/8221 ,  C12Y302/01147

    摘要： According to the present invention, a method for stably accumulating a target protein in plant cells or a plant body, and a transgenic plant in which protein has accumulated, are provided. The method of the present invention is a method for accumulating protein in plant cells, comprising accumulating a target protein or a protein deficient in an N-terminal region of the target protein in vacuoles of myrosin cells present in a multiple mutant, in which myrosin cells deficient in intravacuolar protein are also present in a plant body at locations other than around vascular bundles, by expressing a gene that encodes a target protein having an intracellular membrane system localization signal on the N-terminal and a vacuole localization signal on the C-terminal in the multiple mutant.

    摘要翻译： 根据本发明，提供了用于在植物细胞或植物体中稳定地积聚靶蛋白的方法和其中积累了蛋白质的转基因植物。 本发明的方法是在植物细胞中积聚蛋白质的方法，其特征在于：在存在于多突变体中的黑素瘤细胞的空泡中积聚靶蛋白的N末端区域的靶蛋白或缺失蛋白质的蛋白质， 通过在N末端表达编码具有细胞内膜系统定位信号的靶蛋白的基因和C末端的液泡定位信号，也存在于血管内蛋白质不足的植物体内， 在多重突变体中。

    Broccoli based nutritional supplements
    35.
    发明授权
    Broccoli based nutritional supplements 有权
    西兰花营养补品

    公开(公告)号：US09017666B2

    公开(公告)日：2015-04-28

    申请号：US13910576

    申请日：2013-06-05

    申请人： Jarrow Formulas, Inc.

    发明人： Kean Ashurst

    IPC分类号： A23L1/30

    CPC分类号： A61K36/31 ,  A23L33/105 ,  A61K9/0053 ,  A61K31/375 ,  A61K31/7028 ,  A61K38/47 ,  C12Y302/01147

    摘要： A nutritional supplement is provided containing two broccoli based components that are combined and, when ingested, react in the intestines to provide sulforaphane. Processes for producing the broccoli components of the nutritional supplement are also provided. The broccoli components are treated separately using supercritical fluid extraction to remove oils. The temperature and pressure of the supercritical extraction processes are controlled to provide broccoli components having the desired properties for the nutritional supplement.

    摘要翻译： 提供了一种营养补充剂，其含有两种以西兰花为基础的组分，其组合并且在摄入时在肠中反应以提供萝卜硫素。 还提供了用于生产营养补充剂的花椰菜成分的方法。 使用超临界流体萃取分离处理西兰花组分以除去油。 控制超临界萃取过程的温度和压力以提供具有营养补充剂所需性质的花椰菜成分。

    Process of extracting isothiocyanates
    36.
    发明授权
    Process of extracting isothiocyanates 失效
    提取异硫氰酸酯的方法

    公开(公告)号：US08697150B2

    公开(公告)日：2014-04-15

    申请号：US12750999

    申请日：2010-03-31

    申请人： Athula Ekanayake ,  Scott Alan Vandiest ,  Jeffrey John Kester ,  Paul Henry Zoutendam ,  Jairus R. D. David

    发明人： Athula Ekanayake ,  Scott Alan Vandiest ,  Jeffrey John Kester ,  Paul Henry Zoutendam ,  Jairus R. D. David

    IPC分类号： A61K36/31 ,  A01N65/00

    CPC分类号： A61K36/31 ,  C12P11/00 ,  C12P13/00 ,  C12Y302/01147

    摘要： A process for producing an essential oil. The essential oil can be white mustard essential oil. The white mustard essential oil can include a moisture sensitive isothiocyanate compound. The moisture sensitive isothiocyanate compound can be 4-HBITC. The essential oil can be produced from mustard seed, which can comprise a precursor sinalbin and myrosinase enzyme. The mustard seed can be reduced into a powder. Activation of the myrosinase enzyme by using a water solvent and a promoter to form a slurry can be performed, wherein the myrosinase enzyme catalyzes the production of an essential oil comprising an isothiocyanate from the sinalbin precursor.

    摘要翻译： 一种生产精油的方法。 精油可以是芥末精油。 白芥精油可以包括湿敏性异硫氰酸酯化合物。 水分敏感的异硫氰酸酯化合物可以是4-HBITC。 精油可以由芥子籽生产，其可以包含前体锡胺素和黑芥子酶。 芥菜籽可以还原成粉末。 可以进行使用水溶剂和促进剂来形成浆液来激活黑芥子酶，其中所述黑芥子酶催化从所述西宁酯前体产生包含异硫氰酸酯的精油。

    Polynucleotides encoding a beta-glucosidase and uses thereof
    38.
    发明申请
    Polynucleotides encoding a beta-glucosidase and uses thereof 审中-公开
    编码β-葡糖苷酶的多核苷酸及其用途

    公开(公告)号：US20070011772A1

    公开(公告)日：2007-01-11

    申请号：US10506670

    申请日：2003-03-06

    申请人： Volker Lipka ,  Dierk Scheel ,  Paul Schulze-Lefert ,  Sabine Rosahl ,  Jorn Landtag

    发明人： Volker Lipka ,  Dierk Scheel ,  Paul Schulze-Lefert ,  Sabine Rosahl ,  Jorn Landtag

    IPC分类号： A01H5/00 ,  C12Q1/68 ,  G06F19/00 ,  C07H21/04 ,  C12N15/82 ,  A01H1/00 ,  C12N5/04 ,  C07K14/415 ,  C12N9/48

    CPC分类号： C12Y302/01147 ,  C12N9/2445 ,  C12N15/8282 ,  C12Y302/01021

    摘要： Disclosed are polynucleotides that encode a beta-glucosidase belonging to the plant protein class of family 1 glycoside hydrolases. Furthermore described are recombinant nucleic molecules and vectors containing these polynucleotides and host cells, preferably plant cells that are genetically engineered with these polynucleotides. In addition, transgenic plants are disclosed which show an increased activity of the beta-glucosidase described, preferably leading to an improved resistance against plant pathogens. In another aspect, the present application discloses screening methods for identifying a substrate for said betaglucosidase and methods for the production of plant protection compositions from such substrates or hydrolysis products thereof. Furthermore, methods and uses are disclosed which apply the polynucleotides encoding the beta-glucosidase described in order to improve resistance in plants against plant pathogens.

    摘要翻译： 公开了编码属于1型糖苷水解酶的植物蛋白质类型的β-葡糖苷酶的多核苷酸。 此外还描述了含有这些多核苷酸和宿主细胞的重组核酸分子和载体，优选用这些多核苷酸进行遗传工程改造的植物细胞。 此外，公开了显示所描述的β-葡糖苷酶活性增加的转基因植物，优选导致对植物病原体的改善的抗性。 另一方面，本申请公开了用于鉴定所述β-葡糖苷酶的底物的筛选方法和用于从这种底物或其水解产物生产植物保护组合物的方法。 此外，公开了应用编码所述β-葡糖苷酶的多核苷酸以提高植物对植物病原体抗性的方法和用途。