公开(公告)号：US10076113B2

公开(公告)日：2018-09-18

申请号：US15290825

申请日：2016-10-11

申请人： INSTITUT PASTEUR ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (C.N.R.S)

发明人： Fabrice Chretien ,  Mathilde Latil ,  Shahragim Tajbakhsh

IPC分类号： A01N1/02 ,  A61K35/14 ,  C12N5/077 ,  A61K35/34 ,  C12N5/0797 ,  C12N5/0789 ,  A61K35/12

CPC分类号： A01N1/0284 ,  A01N1/021 ,  A61K35/14 ,  A61K35/28 ,  A61K35/34 ,  A61K2035/124 ,  C12N5/0623 ,  C12N5/0647 ,  C12N5/0659 ,  C12N5/0668 ,  C12N2500/02

摘要： The present invention relates to methods for obtaining stem cells from mammalian cadavers, methods for obtaining or purifying stem cells from a sample likely to contain non-stem cells, methods of regeneration of injured tissues and methods of treatment.

公开(公告)号：US20180112184A1

公开(公告)日：2018-04-26

申请号：US15577633

申请日：2016-05-27

申请人： CHA BIOTECH CO., LTD.

发明人： Hye Sun KIM ,  Ah Reum KANG ,  Hyun Ju KIM ,  Kung Mi PARK ,  Sang Un JOO

IPC分类号： C12N5/0775 ,  A61L27/36 ,  A61K35/50

CPC分类号： C12N5/0665 ,  A61K35/50 ,  A61L27/3604 ,  A61L27/3683 ,  A61L27/3687 ,  A61L27/3691 ,  C12N5/0605 ,  C12N2500/02 ,  C12N2501/11 ,  C12N2501/119 ,  C12N2501/12 ,  C12N2501/15 ,  C12N2501/165 ,  C12N2501/70 ,  C12N2501/91

摘要： Enhanced postnatal adherent cells and a preparation method therefor are provided. The preparation method of enhanced postnatal adherent cells can increase the yield and the proliferation rate of adherent cells from placental tissues; and prepare adherent cells, which secrete proteins effective for neurological diseases and have an improved ability for movement to damaged tissues.

公开(公告)号：US20180051251A1

公开(公告)日：2018-02-22

申请号：US15531163

申请日：2016-02-08

申请人： KAO CORPORATION

发明人： Takayoshi INOUE ,  Akira HACHIYA

IPC分类号： C12N5/071

CPC分类号： C12N5/0633 ,  C12N2500/02

摘要： Cultured sebaceous gland cells capable of accumulating lipid droplets therein are provided. A method for producing mature human sebaceous gland cells, comprising culturing immature human sebaceous gland cells under hypoxic conditions.

公开(公告)号：US09868935B2

公开(公告)日：2018-01-16

申请号：US14397122

申请日：2013-04-24

申请人： CRIOESTAMINAL, SAÚDE E TECNOLOGIA, SA.

发明人： Lino Da Silva Ferreira ,  Isabel Maria Fidalgo Dos Santos Silva Carvalho

IPC分类号： C12N5/0789 ,  A61K47/42 ,  A61K47/24 ,  A61K9/06 ,  A01N1/02 ,  A61K35/28

CPC分类号： C12N5/0647 ,  A01N1/0226 ,  A61K9/06 ,  A61K35/28 ,  A61K47/24 ,  A61K47/42 ,  C12N2500/02 ,  C12N2500/36 ,  C12N2501/999 ,  C12N2533/56

摘要： The present disclosure describes a method and composition for enhancing the survival of hematopoietic stem cells, preferably CD34+ derived from human umbilical cord or peripheral blood, in hypoxic and serum-deprived conditions by cultivating the cells in medium containing lysophosphatidic acid, preferably further comprising a gel, namely a biomimetic gel.The method and composition may be used in medicine or cosmetic application, in particular, in treatment of cardiac tissue and/or cardiac diseases, and/or in the treatment of wound healing namely diabetic wound healing.

公开(公告)号：US20170369904A1

公开(公告)日：2017-12-28

申请号：US15629240

申请日：2017-06-21

申请人： Xcell Biosciences, Inc.

发明人： James LIM ,  Luke CASSEREAU

IPC分类号： C12N15/87 ,  C12N5/0783 ,  C12N15/88 ,  C12N5/077 ,  C12N5/074

CPC分类号： C12N15/87 ,  C12N5/0638 ,  C12N5/0656 ,  C12N5/0696 ,  C12N15/88 ,  C12N2500/02 ,  C12N2500/90 ,  C12N2510/00 ,  C12N2527/00

摘要： The present invention describes a method for increasing transfection efficiency of cells. The present invention further provides a method for increasing the efficiency of stem cell reprogramming.

公开(公告)号：US09845457B2

公开(公告)日：2017-12-19

申请号：US14981533

申请日：2015-12-28

申请人： Cedars-Sinai Medical Center

发明人： Eduardo Marbán ,  Tao-Sheng Li

IPC分类号： C12N5/00 ,  C12N5/02 ,  C12N5/0775 ,  C12N5/077

CPC分类号： C12N5/0662 ,  C12N5/0657 ,  C12N2500/02 ,  C12N2500/38 ,  C12N2501/998

摘要： The present application relates to methods and compositions for the generation of therapeutic cells having reduced incidence of karyotypic abnormalities. In several embodiments cardiac stem cells are cultured in an antioxidant-supplemented media that reduces levels of reactive oxygen species, but does not down regulate DNA repair mechanisms. In several embodiments, physiological oxygen concentrations are used during culture in order to increase the proliferation of stem cells, decrease the senescence of the cells, decrease genomic instability, and/or augment the functionality of such cells for cellular therapies.

公开(公告)号：US20170159023A1

公开(公告)日：2017-06-08

申请号：US15411642

申请日：2017-01-20

申请人： Korea Research Institute of Bioscience and Biotechnology

发明人： Yee Sook Cho ,  Kyeong Lee ,  Jung Woon Lee

IPC分类号： C12N5/074 ,  C07D405/12 ,  C12N5/0735 ,  C07D209/18

CPC分类号： C12N5/0696 ,  C07D209/18 ,  C07D209/20 ,  C07D209/24 ,  C07D405/12 ,  C12N5/0606 ,  C12N2500/02 ,  C12N2500/30 ,  C12N2501/115 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/09

摘要： Provided herein are novel indoleacrylic acid-based compounds, and pharmaceutically acceptable salts thereof, useful for the production, maintenance and proliferation of pluripotent stem cells. Also provided are cell culture compositions comprising these compounds, and methods of using these compounds in the production and maintenance of pluripotent stem cells.

公开(公告)号：US20170152482A1

公开(公告)日：2017-06-01

申请号：US15309531

申请日：2015-05-08

申请人： Thankstem S.R.L.

发明人： Marco POLETTINI

IPC分类号： C12N5/0789 ,  A61K35/14

CPC分类号： C12N5/0647 ,  A61K33/40 ,  A61K35/14 ,  A61K35/28 ,  A61K2035/124 ,  C12N2500/02 ,  C12N2501/22

摘要： A method for expanding adult stem cells from blood is provided. The method includes growing and de-programming adult blood stem cells of a blood sample which has been obtained by in vitro treatment of the blood sample with macrophage colony stimulating factor (MCSF), and ozonization of the blood sample.

公开(公告)号：US20170121677A1

公开(公告)日：2017-05-04

申请号：US15179175

申请日：2016-06-10

申请人： Massachusetts Institute of Technology

发明人： Clark K. Colton ,  Jeffrey R. Millman

IPC分类号： C12N5/0735 ,  C12N5/074

CPC分类号： C12N5/0606 ,  C12N5/0696 ,  C12N2500/02

摘要： The invention provides methods and compositions relating to differentiated cell populations that derive from pluripotent stem cells. The methods relate to reducing the number of residual stem cells present in such populations. The compositions include differentiated cell populations that contain reduced number of stem cells or that contain no stem cells. Pluripotent stem cells may be reduced in number and/or function through exposure to low oxygen levels.

公开(公告)号：US09587225B2

公开(公告)日：2017-03-07

申请号：US14358672

申请日：2012-11-08

申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY

发明人： Yee Sook Cho ,  Kyeong Lee ,  Jung Woon Lee

IPC分类号： C12N5/00 ,  C12N5/074 ,  C07D209/20 ,  C07D209/18 ,  C07D209/24 ,  C07D405/12

CPC分类号： C12N5/0696 ,  C07D209/18 ,  C07D209/20 ,  C07D209/24 ,  C07D405/12 ,  C12N5/0606 ,  C12N2500/02 ,  C12N2500/30 ,  C12N2501/115 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/09

摘要： Provided herein are novel indoleacrylic acid-based compounds, and pharmaceutically acceptable salts thereof, useful for the production, maintenance and proliferation of pluripotent stem cells. Also provided are cell culture compositions comprising these compounds, and methods of using these compounds in the production and maintenance of pluripotent stem cells.

摘要翻译： 本文提供了可用于多能干细胞的产生，维持和增殖的新颖的基于吲哚丙烯酸的化合物及其药学上可接受的盐。 还提供了包含这些化合物的细胞培养组合物，以及在多能干细胞的生产和维持中使用这些化合物的方法。