公开(公告)号：US20210291135A1

公开(公告)日：2021-09-23

申请号：US17338576

申请日：2021-06-03

Applicant: Illumina, Inc.

Inventor： Steven M. Barnard ,  M. Shane Bowen ,  Maria Candelaria Rogert Bacigalupo ,  Wayne N. George ,  Andrew A. Brown ,  James Tsay

IPC: B01J19/00 ,  C12N15/10 ,  C12Q1/6837

Abstract: Provided is an array including a solid support having a surface, the surface having a plurality of wells, the wells containing a gel material, the wells being separated from each other by interstitial regions on the surface, the interstitial regions segregating the gel material in each of the wells from the gel material in other wells of the plurality; and a library of target nucleic acids in the gel material, wherein the gel material in each of the wells comprises a single species of the target nucleic acids of the library. Methods for making and using the array are also provided.

公开(公告)号：US10975210B2

公开(公告)日：2021-04-13

申请号：US15989079

申请日：2018-05-24

Applicant: Illumina, Inc.

Inventor： Lorenzo Berti ,  Andrew A. Brown ,  Wayne N. George

IPC: C08J7/12 ,  C12Q1/6876 ,  C08F8/00 ,  C12Q1/6874 ,  B01J19/00 ,  C09D133/26 ,  C12Q1/6837 ,  C08F220/60

Abstract: The present application relates to a method for preparing a functionalized surface and the use in DNA sequencing and other diagnostic applications. A substrate with a surface comprising a silane or a silane derivative covalently attached to optionally substituted cycloalkene or optionally substituted heterocycloalkene can be used for direct conjugation with a functionalized molecule of interest, such as a polymer, a hydrogel, an amino acid, a nucleoside, a nucleotide, a peptide, a polynucleotide, or a protein. In some embodiments, the silane or silane derivative contains optionally substituted norbornene or norbornene derivatives.

公开(公告)号：US20200024661A1

公开(公告)日：2020-01-23

申请号：US16515790

申请日：2019-07-18

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Timothy J. Merkel ,  Wayne N. George ,  Andrew A. Brown ,  Audrey Zak ,  Gianluca Andrea Artioli ,  Julia Morrison ,  Nikolai Romanov ,  Lorenzo Berti ,  Graham Boud

IPC: C12Q1/6874 ,  C08G77/442 ,  C08L63/00 ,  C12Q1/6876 ,  C09D163/00 ,  C09D153/00

Abstract: An example of a resin composition includes a free radical curable resin matrix including an acrylate and a siloxane, and a free radical photoinitiator. When cured, the resin composition has low or no autofluorescence when exposed to blue excitation wavelengths ranging from about 380 nm to about 480 nm or green excitation wavelengths ranging from about 510 nm to about 560 nm.

公开(公告)号：US10266891B2

公开(公告)日：2019-04-23

申请号：US15680561

申请日：2017-08-18

Applicant: Illumina, Inc.

Inventor： Wayne N. George ,  Andrew A. Brown ,  Daniel Bratton ,  Hongji Ren ,  Ryan C. Smith

IPC: C12Q1/68 ,  C12Q1/6874 ,  B05D3/06 ,  C09D133/26 ,  C09D135/00 ,  C12Q1/6806

Abstract: The present disclosure relates to polymer coatings covalently attached to the surface of a substrate and the preparation of the polymer coatings, such as poly(N-(5-azidoacetamidylpentyl)acrylamide-co-acrylamide) (PAZAM), in the formation and manipulation of substrates, such as molecular arrays and flow cells. The present disclosure also relates to methods of preparing a substrate surface by using beads coated with a covalently attached polymer, such as PAZAM, and the method of determining a nucleotide sequence of a polynucleotide attached to a substrate surface described herein.

公开(公告)号：US20190046943A1

公开(公告)日：2019-02-14

申请号：US16118307

申请日：2018-08-30

Applicant: Illumina, Inc.

Inventor： Steven M. Barnard ,  M. Shane Bowen ,  Maria Candelaria Rogert Bacigalupo ,  Wayne N. George ,  Andrew A. Brown ,  James Tsay

IPC: B01J19/00 ,  C12N15/10 ,  C12Q1/6837

Abstract: An example method includes contacting a substrate coated with a sol-gel material with a stamp that includes a plurality of protruding features. While contacting the coated sol-gel material with the stamp, the example method further includes curing the coated sol-gel material so as to form a patterned sol-gel layer that includes a plurality of wells. The stamp is separated from the patterned sol-gel layer.

公开(公告)号：US09512422B2

公开(公告)日：2016-12-06

申请号：US13787396

申请日：2013-03-06

Applicant: Illumina, Inc.

Inventor： Steven M. Barnard ,  M. Shane Bowen ,  Maria Candelaria Rogert Bacigalupo ,  Wayne N. George ,  Andrew A. Brown ,  James Tsay

IPC: C12N15/10 ,  C12Q1/68 ,  B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00621 ,  B01J2219/00644 ,  B01J2219/00722 ,  C12N15/1068 ,  C12Q1/6837 ,  C12Q2563/159

Abstract: Provided is an array including a solid support having a surface, the surface having a plurality of wells, the wells containing a gel material, the wells being separated from each other by interstitial regions on the surface, the interstitial regions segregating the gel material in each of the wells from the gel material in other wells of the plurality; and a library of target nucleic acids in the gel material, wherein the gel material in each of the wells comprises a single species of the target nucleic acids of the library. Methods for making and using the array are also provided.

Abstract translation: 提供了一种阵列，其包括具有表面的固体支持物，所述表面具有多个孔，所述孔包含凝胶材料，所述孔通过表面上的间隙区彼此分离，所述间隙区域在每个孔中分离凝胶材料 的来自多个其他孔中的凝胶材料的孔; 和凝胶材料中的靶核酸文库，其中每个孔中的凝胶材料包含文库的单个靶核酸的种类。 还提供了制作和使用阵列的方法。

公开(公告)号：US20150005447A1

公开(公告)日：2015-01-01

申请号：US14316478

申请日：2014-06-26

Applicant: Illumina, Inc.

Inventor： Lorenzo Berti ,  Andrew A. Brown ,  Wayne N. George

IPC: C08J7/12 ,  C12Q1/68 ,  C08F22/38

CPC classification number: C08J7/12 ,  B01J19/0046 ,  B01J2219/00529 ,  B01J2219/00533 ,  B01J2219/00619 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00722 ,  C08F8/00 ,  C08F2220/603 ,  C08J2335/00 ,  C09D133/26 ,  C12Q1/6837 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2527/125 ,  C12Q2565/537 ,  C12Q2565/629

Abstract: Some embodiments described herein relate to a substrate with a surface comprising a silane or a silane derivative covalently attached to optionally substituted cycloalkene or optionally substituted heterocycloalkene for direct conjugation with a functionalized molecule of interest, such as a polymer, a hydrogel, an amino acid, a nucleoside, a nucleotide, a peptide, a polynucleotide, or a protein. In some embodiments, the silane or silane derivative contains optionally substituted norbornene or norbornene derivatives. Method for preparing a functionalized surface and the use in DNA sequencing and other diagnostic applications are also disclosed.

Abstract translation: 本文描述的一些实施方案涉及具有表面的底物，其包含共价连接到任选取代的环烯烃或任选取代的杂环烯烃的硅烷或硅烷衍生物，用于与目标官能化分子直接缀合，例如聚合物，水凝胶，氨基酸， 核苷，核苷酸，肽，多核苷酸或蛋白质。 在一些实施方案中，硅烷或硅烷衍生物含有任选取代的降冰片烯或降冰片烯衍生物。 还公开了制备官能化表面的方法以及DNA测序和其他诊断应用中的用途。

公开(公告)号：US20140243224A1

公开(公告)日：2014-08-28

申请号：US13787396

申请日：2013-03-06

Applicant: ILLUMINA, INC.

Inventor： Steven M. Barnard ,  M. Shane Bowen ,  Maria Candelaria Rogert Bacigalupo ,  Wayne N. George ,  Andrew A. Brown ,  James Tsay

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00621 ,  B01J2219/00644 ,  B01J2219/00722 ,  C12N15/1068 ,  C12Q1/6837 ,  C12Q2563/159

Abstract: Provided is an array including a solid support having a surface, the surface having a plurality of wells, the wells containing a gel material, the wells being separated from each other by interstitial regions on the surface, the interstitial regions segregating the gel material in each of the wells from the gel material in other wells of the plurality; and a library of target nucleic acids in the gel material, wherein the gel material in each of the wells comprises a single species of the target nucleic acids of the library. Methods for making and using the array are also provided.

Abstract translation: 提供了一种阵列，其包括具有表面的固体支持物，所述表面具有多个孔，所述孔包含凝胶材料，所述孔通过表面上的间隙区彼此分离，所述间隙区域在每个孔中分离凝胶材料 的来自多个其他孔中的凝胶材料的孔; 和凝胶材料中的靶核酸文库，其中每个孔中的凝胶材料包含文库的单个靶核酸的种类。 还提供了制作和使用阵列的方法。

公开(公告)号：US20250163505A1

公开(公告)日：2025-05-22

申请号：US18947955

申请日：2024-11-14

Applicant: Illumina, Inc.

Inventor： Nam Nguyen ,  Iuliana Petruta Maria ,  Andrew A. Brown ,  Wayne N. George ,  Xiaolin Wu ,  Xavier Von Hatten ,  Madushani Dharmarwardana

IPC: C12Q1/6874 ,  C08F134/04 ,  G01N33/58

Abstract: Embodiments of the present disclosure relate to compositions and methods for labeling of nucleotides, oligonucleotides, or polynucleotides with conjugated polymers or polymer dots. In particular, conjugated polymers or polymer dots can be used as detectable labels for nucleotides, oligonucleotides, or polynucleotides in sequencing by synthesis.

公开(公告)号：US20240337582A1

公开(公告)日：2024-10-10

申请号：US18744225

申请日：2024-06-14

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Timothy J. Merkel ,  Wayne N. George ,  Andrew A. Brown ,  Audrey Zak ,  Gianluca Andrea Artioli ,  Julia Morrison ,  Nikolai Romanov ,  Lorenzo Berti ,  Graham Boud

IPC: G01N15/1434 ,  C08L63/00 ,  C08L83/12 ,  G03F7/00

CPC classification number: G01N15/1436 ,  C08L63/00 ,  C08L83/12 ,  G03F7/0002

Abstract: An example of a resin composition includes an epoxy resin matrix, a free radical photoinitiator selected from the group consisting of 2-ethyl-9,10-dimethoxyanthracene, 2,2-dimethoxy-2-phenylacetophenone, 2-ethoxy-2-phenylacetophenone, and a phosphine oxide, and a photoacid generator. When cured, the resin composition has low or no autofluorescence when exposed to blue excitation wavelengths ranging from about 380 nm to about 480 nm or green excitation wavelengths ranging from about 510 nm to about 560 nm.