公开(公告)号：US09128124B2

公开(公告)日：2015-09-08

申请号：US13764612

申请日：2013-02-11

Applicant: California Institute of Technology

Inventor： Aditya Rajagopal ,  Chieh-Feng Chang ,  Akram Sarwat Sadek ,  Axel Scherer ,  Raymond Jimenez

IPC: G01R19/20 ,  G01R19/00 ,  A61B5/04 ,  A61B5/00

CPC classification number: G01R19/00 ,  A61B5/0031 ,  A61B5/04001 ,  A61B2560/0214 ,  A61B2562/0209 ,  A61B2562/0233 ,  A61B2562/164 ,  A61B2562/166 ,  A61B2562/223

Abstract: A voltage sensing apparatus on a semiconductor substrate, including one or more inputs comprising metal contacts, an output comprising a laser transmitter, circuitry electrically connecting and interfacing the inputs to the output; and a power module. A method of fabricating the apparatus is also described.

Abstract translation: 一种半导体衬底上的电压感测装置，包括一个或多个包括金属触点的输入端，包括激光发射器的输出端，将输入电连接到输出端的电路; 和电源模块。 还描述了制造该装置的方法。

公开(公告)号：US08707545B2

公开(公告)日：2014-04-29

申请号：US14038702

申请日：2013-09-26

Applicant: California Institute of Technology

Inventor： Samson Chen ,  Aditya Rajagopal ,  Axel Scherer

IPC: G01N15/00

CPC classification number: H05K3/10 ,  B01L3/502707 ,  B01L3/502715 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  H01L23/473 ,  H01L2924/0002 ,  H05K1/0272 ,  H05K3/284 ,  H05K2201/10189 ,  H05K2203/1327 ,  Y10T29/49 ,  Y10T29/49002 ,  Y10T29/49155 ,  Y10T29/49165 ,  Y10T137/85938 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/2575 ,  H01L2924/00

Abstract: A printed circuit board structure is coated with an encapsulant within which microfluidic channels have been formed. The microfluidic channels are formed by soldering fluidic connections to metal traces on a surface of the printed circuit board structure prior to encapsulation. The metal traces are removed by etching after encapsulation to form microchannels within the encapsulant.

Abstract translation: 印刷电路板结构涂覆有已经形成微流体通道的密封剂。 在封装之前，通过将流体连接焊接到印刷电路板结构的表面上的金属迹线来形成微流体通道。 在封装后通过蚀刻去除金属迹线，以在密封剂内形成微通道。

公开(公告)号：US12168797B2

公开(公告)日：2024-12-17

申请号：US18352112

申请日：2023-07-13

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Emil P. Kartalov ,  Aditya Rajagopal ,  Axel Scherer

IPC: G01N33/48 ,  C12Q1/68 ,  C12Q1/6825 ,  C12Q1/6851 ,  G01N21/64 ,  G01N33/50 ,  G16B5/00 ,  G16B25/00 ,  G16B25/20 ,  G16B40/10 ,  G06F17/10 ,  G06F17/11 ,  G16B40/00

Abstract: This disclosure provides methods, systems, compositions, and kits for the multiplexed detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, systems, compositions, and kits wherein multiple analytes may be detected in a single sample volume by acquiring a cumulative measurement or measurements of at least one quantifiable component of a signal. In some cases, additional components of a signal, or additional signals (or components thereof) are also quantified. Each signal or component of a signal may be used to construct a coding scheme which can then be used to determine the presence or absence of any analyte.

公开(公告)号：US20240167092A1

公开(公告)日：2024-05-23

申请号：US18128764

申请日：2023-03-30

Applicant: California Institute of Technology

Inventor： Emil P. Kartalov ,  Aditya Rajagopal ,  Axel Scherer ,  Mark D. Goldberg

IPC: C12Q1/6883 ,  C12Q1/68 ,  C12Q1/6851 ,  C12Q1/70

CPC classification number: C12Q1/6883 ,  C12Q1/68 ,  C12Q1/6851 ,  C12Q1/701 ,  C12Q1/703 ,  C12Q2600/156 ,  Y10T436/143333

Abstract: FRET-based analytes detection and related methods and systems are described where a pair of FRET labeled primers and/or oligonucleotides are used that are specific for target sequences located at a distance up to four time the Forster distance of the FRET chromophores presented on the FRET labeled primers and/or oligonucleotides one with respect to the other in one or more polynucleotide analyte; in particular the pair of FRET labeled primers and/or oligonucleotides is combined with a sample and subjected to one or more polynucleotide amplification reactions before measuring FRET signals from at least one FRET chromophore.

公开(公告)号：US11827921B2

公开(公告)日：2023-11-28

申请号：US16864744

申请日：2020-05-01

Applicant: California Institute of Technology

Inventor： Emil P. Kartalov ,  Aditya Rajagopal ,  Axel Scherer

IPC: G01N33/48 ,  G01N33/50 ,  C12Q1/6825 ,  G16B25/00 ,  G16B5/00 ,  C12Q1/6851 ,  G16B25/20 ,  G16B40/10 ,  C12Q1/68 ,  G01N21/64 ,  G06F17/10 ,  G06F17/11 ,  G16B40/00

CPC classification number: C12Q1/6825 ,  C12Q1/68 ,  C12Q1/6851 ,  G01N21/6486 ,  G16B5/00 ,  G16B25/00 ,  G16B25/20 ,  G16B40/10 ,  G06F17/10 ,  G06F17/11 ,  G16B40/00 ,  Y02A50/30 ,  C12Q1/6851 ,  C12Q2537/101 ,  C12Q2537/143 ,  C12Q2537/165 ,  C12Q2563/107

Abstract: This disclosure provides methods, systems, compositions, and kits for the multiplexed detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, systems, compositions, and kits wherein multiple analytes may be detected in a single sample volume by acquiring a cumulative measurement or measurements of at least one quantifiable component of a signal. In some cases, additional components of a signal, or additional signals (or components thereof) are also quantified. Each signal or component of a signal may be used to construct a coding scheme which can then be used to determine the presence or absence of any analyte.

公开(公告)号：US20230086246A1

公开(公告)日：2023-03-23

申请号：US17885037

申请日：2022-08-10

Applicant: California Institute of Technology

Inventor： Emil P. Kartalov ,  Aditya Rajagopal ,  Axel Scherer

IPC: C12Q1/6825 ,  G16B25/00 ,  G16B5/00 ,  C12Q1/6851 ,  G16B25/20 ,  G16B40/10 ,  C12Q1/68 ,  G01N21/64

Abstract: This disclosure provides methods, systems, compositions, and kits for the multiplexed detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, systems, compositions, and kits wherein multiple analytes may be detected in a single sample volume by acquiring a cumulative measurement or measurements of at least one quantifiable component of a signal. In some cases, additional components of a signal, or additional signals (or components thereof) are also quantified. Each signal or component of a signal may be used to construct a coding scheme which can then be used to determine the presence or absence of any analyte.

公开(公告)号：US11492664B2

公开(公告)日：2022-11-08

申请号：US16051736

申请日：2018-08-01

Applicant: California Institute of Technology

Inventor： Aditya Rajagopal ,  Emil P. Kartalov

IPC: C12Q1/68 ,  C12Q1/686 ,  C07H21/04 ,  G01N21/64

Abstract: The present invention relates to methods of nucleic acid analyte detection by PCR. In particular, methods and kits for the detection of a plurality of nucleic acid analytes and the generation of kinetic signatures are provided. Further provided are methods and kits of nested PCR and PCR using limiting primers.

公开(公告)号：US20210012860A1

公开(公告)日：2021-01-14

申请号：US16937464

申请日：2020-07-23

Applicant: California Institute of Technology

Inventor： Emil P. Kartalov ,  Aditya Rajagopal ,  Axel Scherer

IPC: G16B25/00 ,  G16B5/00 ,  C12Q1/6851 ,  G16B25/20 ,  G16B40/10 ,  C12Q1/68 ,  G01N21/64 ,  C12Q1/6825

Abstract: This disclosure provides methods, systems, compositions, and kits for the multiplexed detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, systems, compositions, and kits wherein multiple analytes may be detected in a single sample volume by acquiring a cumulative measurement or measurements of at least one quantifiable component of a signal. In some cases, additional components of a signal, or additional signals (or components thereof) are also quantified. Each signal or component of a signal may be used to construct a coding scheme which can then be used to determine the presence or absence of any analyte.

公开(公告)号：US10597737B2

公开(公告)日：2020-03-24

申请号：US16109288

申请日：2018-08-22

Applicant: California Institute of Technology

Inventor： Aditya Rajagopal ,  Mark D. Goldberg ,  Erika F. Garcia ,  Xiomara L. Madero ,  Thomas A. Tombrello ,  Axel Scherer

IPC: C12P19/34 ,  C12Q1/70 ,  C12Q1/68 ,  C12Q1/6851

Abstract: Methods and kits for detecting a genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, and the first primer and the second primer are specific for the polynucleotide analyte. At least one of the primers is configured to hybridize to a region of the polynucleotide analyte encoding the genetic variation. The primers are configured to amplify the polynucleotide analyte having the genetic variation and a corresponding polynucleotide analyte lacking the generic variation. There is a detectable difference between a change in signal generated by the fluorophore and quencher when using the first and second primers to amplify the polynucleotide analyte with the genetic variation, and a change in signal generated by the fluorophore and quencher when using the first and second primers to amplify the corresponding polynucleotide analyte lacking the genetic variation.

公开(公告)号：US10524761B2

公开(公告)日：2020-01-07

申请号：US16038070

申请日：2018-07-17

Applicant: California Institute of Technology

Inventor： Aditya Rajagopal ,  Alaina Ann Brinley Rajagopal

IPC: A61B7/04 ,  A61B5/0205 ,  A61B8/00 ,  A61B5/00 ,  G01S15/89 ,  A61B5/145 ,  A61B5/08 ,  A61B5/021 ,  A61B8/02 ,  A61B8/06 ,  A61B8/08

Abstract: An enhanced stethoscope device and method for operating the enhanced stethoscope are provided. The enhanced stethoscope device generally operates by providing stethoscope sensors, ultrasonic sensors, and other sensors to obtain a series of measurements about a subject. The series of measurements may be correlated, such as by machine learning, to extract clinically relevant information. Also described are systems and methods for ultrasonic beamsteering by interference of an audio signal with an ultrasonic signal.