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30 条记录 (耗时 0.052 秒)

    Methods for promoting, inhibiting and detecting toxin entry into cells
    23.
    发明授权
    Methods for promoting, inhibiting and detecting toxin entry into cells 失效
    促进，抑制和检测毒素进入细胞的方法

    公开(公告)号：US07517667B2

    公开(公告)日：2009-04-14

    申请号：US11214997

    申请日：2005-08-30

    申请人： John R. Murphy ,  Ryan Ratts

    发明人： John R. Murphy ,  Ryan Ratts

    IPC分类号： C12P1/00 ,  C12P21/04 ,  C12Q1/02 ,  A61K38/00

    CPC分类号： G01N33/5076 ,  A61K38/08 ,  C07K14/34 ,  C07K14/55 ,  C07K2319/55 ,  G01N33/5011 ,  G01N33/5035 ,  G01N2510/00

    摘要： In vitro delivery of the diphtheria toxin (DT) catalytic (C) domain from the lumen of purified early endosomes to the external milieu requires the addition of both ATP and a cytosolic translocation factor (CTF) complex. The results presented here demonstrate that β-COP plays an essential role in the cytosolic release of the C-domain and is mediated by a consensus peptide sequence found on several bacterial toxins and in HIV-1 reverse transcriptase. The invention features methods for inhibiting cell death that include the administration of compounds based on this consensus sequence that inhibit the translocation of the catalytic domain of toxins or transcription factors. Also featured are methods for identifying compounds that inhibit cell death, and methods for identifying compounds that promote cell death by blocking or accelerating, respectively, the rate of toxin/factor endosomal translocation.

    摘要翻译： 将白喉毒素（DT）催化（C）结构域从纯化的早期内体的内部体外递送到外部环境需要添加ATP和胞质易位因子（CTF）复合物。 这里提出的结果表明β-COP在C-结构域的胞质释放中起重要作用，并且由在几种细菌毒素和HIV-1逆转录酶中发现的共有肽序列介导。 本发明的特征在于抑制细胞死亡的方法，包括基于抑制毒素或转录因子的催化结构域易位的共有序列的化合物的施用。 还特征在于鉴定抑制细胞死亡的化合物的方法，以及通过阻断或加速毒素/因子内体易位的速率来鉴定促进细胞死亡的化合物的方法。

    Identification of virulence determinants activators in prokaryotic pathogens
    24.
    发明授权
    Identification of virulence determinants activators in prokaryotic pathogens 失效
    鉴定原核病原体中毒力决定簇活化剂

    公开(公告)号：US06309817B1

    公开(公告)日：2001-10-30

    申请号：US09408618

    申请日：1999-09-30

    申请人： John R. Murphy ,  Li Sun

    发明人： John R. Murphy ,  Li Sun

    IPC分类号： C12Q100

    CPC分类号： C12N15/1086 ,  C12N15/635 ,  C12Q1/6897

    摘要： Disclosed is a method for identifying activators of a transition metal-dependent repressor of virulence gene expression in infectious prokaryotic pathogens. The method utilizes genetic circuitry that represents the response of a given prokaryote to nutritional stress and the expression of genes that contribute to the establishment of the infectious process. The exposure of recombinant cells or a cell-free system containing the genetic circuitry to a non-metal ion test substance that activates the repressor produces a detectable response. The method is applicable for any prokaryote employing metal ion-dependent repressors to regulate specific gene expression, specifically as it pertains to virulence determinant expression.

    摘要翻译： 公开了用于鉴定感染性原核病原体中毒性基因表达的过渡金属依赖性阻遏物的活化剂的方法。 该方法利用代表给定原核生物对营养压力的反应的遗传回路和有助于建立感染过程的基因的表达。 将包含遗传电路的重组细胞或无细胞系统暴露于激活阻遏物的非金属离子测试物质产生可检测的反应。 该方法适用于任何使用金属离子依赖性阻遏物调节特异性基因表达的原核生物，特别是与毒力决定簇表达有关。

    Optimal solar tracking system
    27.
    发明授权
    Optimal solar tracking system 失效
    最佳太阳跟踪系统

    公开(公告)号：US5816540A

    公开(公告)日：1998-10-06

    申请号：US577436

    申请日：1995-12-22

    申请人： John R. Murphy ,  Ross Crowley

    发明人： John R. Murphy ,  Ross Crowley

    IPC分类号： B64G1/24 ,  B64G1/28 ,  B64G1/36 ,  B64G1/40 ,  B64G1/44

    CPC分类号： B64G1/24 ,  B64G1/36 ,  B64G1/407 ,  B64G1/44 ,  B64G1/28 ,  B64G1/288 ,  B64G1/363

    摘要： A spacecraft traveling in a volume of space receiving radiation from the sun and encountering an undesired force. The spacecraft has a solar panel movably attached to said spacecraft. The spacecraft further includes a solar panel controller which controls the movement of the solar panel, wherein the controller moves the solar panel in a time modulated manner so that a torque is generated which compensates for the undesired force.

    摘要翻译： 航天飞机在太空中接收辐射并遇到不希望有的力的空间。 航天器具有可移动地附接到所述航天器的太阳能电池板。 航天器还包括控制太阳能面板运动的太阳能面板控制器，其中控制器以时间调制的方式移动太阳能电池板，从而产生补偿不期望的力的转矩。

    Payload depolyment from shuttle employing an ejection restraint device
    28.
    发明授权
    Payload depolyment from shuttle employing an ejection restraint device 失效
    使用弹射约束装置的穿梭器的有效载荷分解

    公开(公告)号：US4679752A

    公开(公告)日：1987-07-14

    申请号：US695156

    申请日：1985-01-28

    申请人： Alois Wittmann ,  Theodore M. Isaacs ,  John R. Murphy

    发明人： Alois Wittmann ,  Theodore M. Isaacs ,  John R. Murphy

    IPC分类号： B64G1/64 ,  B64G1/22

    CPC分类号： B64G1/645 ,  B64G1/641

    摘要： Apparatus for use in gyroscopically ejecting a spacecraft supported in the open end of a spacecraft supporting cradle. The invention comprises a separable ejection restraint mechanism attached between the cradle and the spacecraft. The mechanism restrains the thrust force applied to the spacecraft for a predetermined time period in order to dissipate undetermined forces acting upon the spacecraft prior to the ejection thereof. The mechanism comprises a separable resilient portion and a releasable clamp portion coupled thereto. The resilient portion comprises a spring loaded anchor rod arrangement while the releasable clamp portion comprises a spring loaded split ball clamp arrangement. The invention also contemplates the use of a flexible carrier coupled between the spacecraft and the cradle. The invention acts as a tether connected between the cradle and spacecraft which acts to establish a determinant state of force on the cargo and eliminate unwanted ejection-related problems prior to cargo deployment. In addition, the release of a single tether employed as the sole cargo release initiator minimizes the possible imparting of uncontrolled momentum to the cargo due to structural deformation energy.

    摘要翻译： 用于陀螺仪弹射支撑在航天器支架的开口端的航天器的装置。 本发明包括一个连接在支架和航天器之间的可分离的弹出约束机构。 该机构限制施加到航天器的预定时间段的推力，以便在弹射之前消散作用在航天器上的未确定的力。 该机构包括可分离的弹性部分和与其连接的可释放的夹紧部分。 弹性部分包括弹簧加载的锚杆装置，而可释放的夹紧部分包括弹簧加载的分离球形夹具装置。 本发明还考虑使用耦合在航天器和支架之间的柔性载体。 本发明用作连接在摇篮和航天器之间的系绳，其用于在货物部署之前确定货物上的决定性状态并消除不期望的与喷射有关的问题。 此外，作为唯一货物释放引发器使用的单个系绳的释放使由于结构变形能量而可能对货物施加不受控制的动量最小化。

    Compressed air-operated motor employing dual lobe cams
    29.
    发明授权
    Compressed air-operated motor employing dual lobe cams 失效
    采用双凸轮凸轮的压缩式气动马达

    公开(公告)号：US4018050A

    公开(公告)日：1977-04-19

    申请号：US705940

    申请日：1976-07-16

    申请人： John R. Murphy

    发明人： John R. Murphy

    IPC分类号： F01L1/08 ,  F01B1/02 ,  F01B17/02 ,  F01L15/00 ,  F15B11/06

    CPC分类号： F02B21/00 ,  F01B1/02 ,  F01B17/02 ,  F01L15/00

    摘要： An air-operated engine system usable for driving a vehicle by means of compressed air from a rechargeable storage tank. The engine of the system has cylinders containing driving pistons connected to a crankshaft. Compressed air from the storage tank is supplied at regulated pressure to the cylinders for power strokes by means of intake valves and the air is exhausted from the cylinders at the ends of the power strokes by means of exhaust valves. The intake and exhaust valves are operated by dual lobe cams on a camshaft driven from the crankshaft. The air is compressed by a hydrovane compressor which has a constant flow of air operating under 100 pounds pressure with control valves which will take in 25 pounds per minute up to 200 pounds. The output pressure is controlled by the input pressure.

    摘要翻译： 一种用于通过来自可再充电储罐的压缩空气驱动车辆的气动发动机系统。 该系统的发动机具有连接到曲轴的驱动活塞的气缸。 来自储罐的压缩空气通过进气阀以调节的压力供给到用于动力冲程的气缸，并且空气通过排气阀从动力冲程端部的气缸排出。 进气和排气阀由从曲轴驱动的凸轮轴上的双凸轮凸轮操作。 空气由水力压缩机压缩，其具有在100磅压力下运行的恒定流量的空气，控制阀将以每分钟25磅至200磅的速度进行压缩。 输出压力由输入压力控制。