公开(公告)号：US20060024833A1

公开(公告)日：2006-02-02

申请号：US11191799

申请日：2005-07-27

Applicant: Kyle Gee ,  Vladimir Martin

Inventor： Kyle Gee ,  Vladimir Martin

IPC: G01N33/20

CPC classification number: G01N33/84 ,  C07C229/18 ,  C07C311/44 ,  C07D209/14 ,  C07D221/14 ,  C07D265/38 ,  C07D311/82 ,  C07D311/90 ,  C07D413/04 ,  C07K14/195 ,  C07K14/43595 ,  C09B5/60 ,  C09B5/62 ,  C09B11/06 ,  C09B11/08 ,  C09B19/00 ,  C09B19/005 ,  C09B57/08 ,  C09K11/06 ,  C09K2211/1014 ,  G01N33/20 ,  G01N2458/00

Abstract: The present invention provides fluorogenic compounds for the detection of target metal ions wherein the compounds exhibit a Stokes shift greater than 50 nm and the detectable signal is modulated by photoinduced electron transfer (PET). The present compounds consist of three functional elements, the ion sensing moiety (chelating moiety), the reporter moiety (fluorophore or fluorescent protein) and spacer or linker between the sensing and reporter moieties of the present compound that allows for PET upon binding of a metal ion and excitation by an appropriate wavelength.

Abstract translation: 本发明提供用于检测靶金属离子的荧光化合物，其中化合物表现出大于50nm的斯托克斯位移，并且可检测信号通过光诱导电子转移（PET）调节。 本发明化合物由三种功能元件组成，即离子感测部分（螯合部分），报道部分（荧光团或荧光蛋白）以及本发明化合物的感测和报道部分之间的间隔物或接头，其允许在结合金属时的PET 通过适当的波长离子和激发。

公开(公告)号：US08680320B2

公开(公告)日：2014-03-25

申请号：US12636687

申请日：2009-12-11

Applicant: Kyle Gee ,  Jolene Bradford ,  Vladimir Martin

Inventor： Kyle Gee ,  Jolene Bradford ,  Vladimir Martin

IPC: C07C229/00 ,  C07C321/00

CPC classification number: C07D413/04 ,  C07D209/14 ,  C07D335/12 ,  C09B11/28

Abstract: The present invention provides a metal chelator and methods that facilitate binding, detecting, monitoring and quantitating of heavy metal ions in a sample. This metal chelating moiety is a —N,N,O-triacetic acid analog of BAPTA and has the following formula

Abstract translation: 本发明提供金属螯合剂和促进样品中重金属离子结合，检测，监测和定量的方法。 该金属螯合部分是BAPTA的-N，N，O-三乙酸类似物，具有下式

公开(公告)号：US20050014197A1

公开(公告)日：2005-01-20

申请号：US10821522

申请日：2004-04-09

Applicant: Brian Agnew ,  Kyle Gee ,  Vladimir Martin

Inventor： Brian Agnew ,  Kyle Gee ,  Vladimir Martin

IPC: C12Q1/48 ,  G01N33/53

CPC classification number: G01N33/5008 ,  G01N33/502 ,  G01N33/5091 ,  G01N2333/4709

Abstract: The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.

Abstract translation: 本发明涉及用于结合，检测和分离磷酸化靶分子的磷酸盐结合化合物，包括随后鉴定与磷酸化靶分子或能够被磷酸化的分子相互作用的靶分子。 提供的结合溶液包括磷酸盐结合化合物，酸和金属离子，其中金属离子同时与靶分子上暴露的磷酸基团相互作用，并且磷酸盐结合化合物的金属螯合部分形成在 磷酸化结合化合物和导致三元复合物的磷酸化靶分子。 本发明的结合溶液用于结合和检测固定和溶解的磷酸化靶分子，从复杂混合物中分离磷酸化靶分子，并辅助蛋白质组分析，其中可以鉴定激酶和磷酸酶底物和酶。