WAVELENGTH-RESOLVING AND HIGH SPATIAL RESOLUTION FLUORESCENCE MICROSCOPY
    251.
    发明申请
    WAVELENGTH-RESOLVING AND HIGH SPATIAL RESOLUTION FLUORESCENCE MICROSCOPY 有权
    波长分辨率和高空间分辨率荧光显微镜

    公开(公告)号:US20130228704A1

    公开(公告)日:2013-09-05

    申请号:US13774539

    申请日:2013-02-22

    Abstract: A method for wavelength-resolving and high spatial resolution fluorescence microscopy in which fluorescence labels in a sample are repeatedly excited to emit fluorescence radiation and frames including images of isolated labels are produced with a microscope. The positions of the images of the isolated fluorescing labels are localized with a localization precision exceeding the optical resolution of the imaging beam path of the microscope. The imaging beam path of the microscope has a diffractive element which, during the imaging, diffracts the image of the sample comprising the isolated fluorescing labels into a first diffraction order so that each frame contains the first diffraction order images of the isolated fluorescing labels. A parameter of the first diffraction order images of the isolated fluorescing labels is evaluated and an indication of the wavelength of the isolated fluorescing labels is derived from this evaluated parameter.

    Abstract translation: 一种用于波长分辨和高空间分辨率荧光显微镜的方法,其中样品中的荧光标记被重复激发以发射荧光辐射,并且使用显微镜产生包括分离标记图像的框架。 分离的荧光标记的图像的位置以超过显微镜的成像光束路径的光学分辨率的定位精度进行定位。 显微镜的成像光束路径具有衍射元件,其在成像期间将包含分离的荧光标记的样品的图像衍射成第一衍射级,使得每个帧包含分离的荧光标记的第一衍射级图像。 评估分离的荧光标记的第一衍射级图像的参数,并从该评估参数导出分离的荧光标记的波长的指示。

    Fabrication process of a microfabricated blazed grating
    253.
    发明授权
    Fabrication process of a microfabricated blazed grating 有权
    微加工闪光光栅的制作工艺

    公开(公告)号:US08465660B2

    公开(公告)日:2013-06-18

    申请号:US12161640

    申请日:2007-01-22

    CPC classification number: G01J3/1804 G02B5/1828 G02B26/0808 H01S5/141

    Abstract: A blazed grating is disclosed as well as mode hop-free tunable lasers and a process for fabricating gratings of this type. The grating lies in a general plane and includes a plurality of elongate beams carrying mutually parallel respective reflection surfaces spaced apart from one another with a predefined pitch, each of these reflection surfaces having a normal direction inclined at a grating angle α to the normal direction of the general plane. The grating includes a plurality of resilient suspension arms connected to the beams and intended to be fastened to a grating support. A first pair of comb electrodes is provided for applying a mechanical force to this assembly, being placed on a first side of the grating, along an axis transverse to the beams, and designed so as to allow the pitch of the grating to be modified in response to the application of the mechanical force.

    Abstract translation: 公开了一种闪耀的光栅以及无跳变可调激光器以及用于制造这种类型的光栅的工艺。 光栅位于通用平面中,并且包括多个细长梁,其承载相互平行的各自的反射表面,彼此以预定的间距彼此间隔开,每个反射面具有以与法线方向成α角的光栅角度倾斜的法线方向 一般飞机。 光栅包括连接到光束并且被固定到光栅支架上的多个弹性悬挂臂。 第一对梳状电极被设置用于沿着横向于梁的轴线向该组件施加机械力,该机械力被放置在光栅的第一侧上,并被设计成允许光栅的间距在 响应机械力的应用。

    Broad Band Czerny-Turner Spectrometer, Methods, and Applications
    254.
    发明申请
    Broad Band Czerny-Turner Spectrometer, Methods, and Applications 有权
    宽带Czerny-Turner光谱仪,方法和应用

    公开(公告)号:US20130044313A1

    公开(公告)日:2013-02-21

    申请号:US13695298

    申请日:2011-04-29

    CPC classification number: G01J3/1804 G01J3/02 G01J3/0208 G01J3/0218 G01J3/10

    Abstract: A low-cost optics, broadband, astigmatism-corrected practical spectrometer. An off-the-shelf cylindrical lens is used to remove astigmatism over the full bandwidth, providing better than 0.1 nm spectral resolution and more than 50% through-put over a bandwidth of 400 nm centered at 800 nm. The spectrometer includes a first spherical mirror disposed along an optical path in an off-axis (tilted) orientation; a diffraction grating disposed along the optical axis in a location optically downstream from the first mirror; a second spherical mirror disposed along the optical path in an off-axis orientation in a location optically down-stream from the diffraction grating; a cylindrical optic disposed in the optical path; and a detector disposed in the optical path in a location optically downstream from the second spherical mirror.

    Abstract translation: 低成本的光学,宽带,散光校正实用光谱仪。 一个现成的柱面透镜用于去除全带宽上的散光,提供超过0.1nm的光谱分辨率,并且以800nm为中心的400nm的带宽上的超过50%的透过率。 光谱仪包括沿着离轴(倾斜)取向的光路布置的第一球面镜; 衍射光栅,沿着光轴布置在第一反射镜光学下游的位置; 在离开衍射光栅的光学下游的位置处以离轴取向沿光路设置的第二球面镜; 设置在光路中的圆柱形光学器件; 以及检测器,设置在所述光路中,在所述第二球面镜的光学下游的位置。

    SPECTRAL DECOMPOSITION DEVICE AND MANUFACTURING THE SAME
    255.
    发明申请
    SPECTRAL DECOMPOSITION DEVICE AND MANUFACTURING THE SAME 有权
    光谱分解装置及其制造方法

    公开(公告)号:US20120236382A1

    公开(公告)日:2012-09-20

    申请号:US13231094

    申请日:2011-09-13

    Abstract: For achieving balance between manufacturing effort and spectrometer accuracy, a spectral decomposition device is not completely integrated into a substrate stack, but, for example, after manufacturing the substrate stack in the manufacturing process, the opportunity of compensating inaccuracies in substrate stack manufacturing is given by mounting a component with a suitable optical functional element to a window, like, e.g., an entry, exit or intermediate window of the substrate stack, to at least partially cover the respective window, wherein the optical functional element is, for example, an entry aperture, an exit aperture or also part of an optics or an optical element having a spectrally decomposing effect. The substrate stack may be manufactured on wafer level and the manufacturing tolerances in this manufacturing may be loosened, as the subsequent substrate stack-individual mounting or even window-individual mounting of the components may compensate the fluctuations which resulted in substrate stack manufacturing.

    Abstract translation: 为了实现制造工作和光谱仪精度之间的平衡,光谱分解装置未完全集成到衬底叠层中,但是例如在制造过程中制造衬底叠层之后,补偿衬底堆叠制造中的不准确性的机会由 将具有合适的光学功能元件的部件安装到诸如基板堆叠的入口,出口或中间窗口的窗口,以至少部分地覆盖相应的窗口,其中光学功能元件例如是入口 孔径,出射孔径或者具有光谱分解效果的光学元件或光学元件的一部分。 可以在晶片级制造衬底叠层,并且可以松开该制造中的制造公差,因为随后的衬底叠层单独安装或甚至窗户单独安装组件可补偿导致衬底堆叠制造的波动。

    Spectral analysis unit with a diffraction grating
    257.
    发明授权
    Spectral analysis unit with a diffraction grating 有权
    具有衍射光栅的光谱分析单元

    公开(公告)号:US07852474B2

    公开(公告)日:2010-12-14

    申请号:US11785153

    申请日:2007-04-16

    Abstract: A spectral analytical unit for acting on a parallel light bundle having different wavelengths. The spectral analytical unit includes a diffraction grating on which the light bundle falls, the diffraction grating splitting the different wavelengths through diffraction in first spectral directions defining a light bundle diffraction order 1 without recycle, and the diffraction grating bending the light bundle in second directions defining a light bundle diffraction order 0 without recycle, a detector line made up of a plurality of elements, optics for focusing the split light bundle diffraction order 1 without recycle on the detector line, evaluation electronics connected to the detector line for obtaining data related to a created spectrum, and a deflecting device wherein the diffraction order 0 light bundle without recycle meets on the deflecting device which is so directed and positioned that this light bundle falls on the diffraction grating thereby creating a reflected diffraction order 1 light bundle with first recycle and a reflected diffraction order 0 light bundle with first recycle whereby the diffraction order 1 without recycle and the reflected diffraction order 1 light bundle with first recycle each of a part wavelength range are impressed through the optics on a single element of the detector line.

    Abstract translation: 用于作用于具有不同波长的平行光束的光谱分析单元。 光谱分析单元包括光束落在其上的衍射光栅,衍射光栅通过在限定光束衍射级数1而不进行再循环的第一光谱方向上通过衍射分离不同波长,并且衍射光栅在限定第二方向的第二方向上折射光束 没有再循环的光束衍射级0,由多个元件组成的检测器线,用于将分束光束衍射级1聚焦在检测器线路上而不再循环的光学器件,连接到检测器线的评估电子设备,用于获得与 以及偏转装置,其中没有再循环的衍射级0光束在偏转装置上相遇,所述偏转装置被定向和定位成使得该光束落在衍射光栅上,从而产生具有第一再循环的反射衍射级1光束和 反射衍射级0光束与fi 第一次循环,其中没有再循环的衍射级1和具有部分波长范围的第一次再循环的反射衍射级1光束通过检测器线的单个元件上的光学器件施加。

    High-resolution spectrally adjustable filter
    258.
    发明授权
    High-resolution spectrally adjustable filter 有权
    高分辨率光谱可调滤镜

    公开(公告)号:US07817272B2

    公开(公告)日:2010-10-19

    申请号:US12135354

    申请日:2008-06-09

    Abstract: For spectrally filtering at least one input beam, a first reflective element is configured to tilt to multiple tilt orientations that each corresponds to a different angle of propagation of at least one input beam. One or more optical elements are configured to change at least some of the relative angles of propagation of the input beam for different tilt orientations of the first reflective element. A spectrally dispersive element is configured to receive the input beam at a location at which the central ray of the input beam is incident at different points on the spectrally dispersive element for each of the tilt orientations, and configured to disperse spectral components of the input beam at different respective angles in a spectral plane. The first reflective element is configured to tilt to select at least one and fewer than all of the dispersed spectral components to be directed to a selected output path.

    Abstract translation: 为了对至少一个输入光束进行光谱滤波,第一反射元件被配置为倾斜到多个倾斜取向,其各自对应于至少一个输入光束的不同传播角度。 一个或多个光学元件被配置为改变用于第一反射元件的不同倾斜取向的输入光束的相对传播角度的至少一些。 光谱分散元件被配置为在输入光束的中心射线入射到用于每个倾斜取向的光谱色散元件上的不同点处的位置处接收输入光束,并且被配置为分散输入光束的光谱分量 在光谱平面中以不同的相应角度。 第一反射元件被配置为倾斜以选择要被引导到所选择的输出路径的至少一个且少于所有分散的光谱分量。

    Method and apparatus for optical spectrum analysis
    259.
    发明授权
    Method and apparatus for optical spectrum analysis 失效
    光谱分析方法和装置

    公开(公告)号:US07755758B2

    公开(公告)日:2010-07-13

    申请号:US11731103

    申请日:2007-03-30

    Inventor: Rüdiger Mästle

    CPC classification number: G01J3/1804 G01J3/02 G01J3/027 G01J9/00

    Abstract: The present invention relates to an apparatus and to a method of optical spectrum analysis of an optical spectrum of a light beam (4), comprising the steps of selecting a certain part of the optical spectrum of the light beam (4) to provide a filtered light beam (12), detecting the filtered light beam (12) to provide an electrical signal corresponding to a power amplitude (O(λ)) of the filtered light beam (12), detecting the filtered light beam (12) to provide an electrical signal corresponding to a wavelength of the filtered light beam (12), and providing the power amplitude as a function of the wavelength of the filtered light beam (12).

    Abstract translation: 本发明涉及光束(4)的光谱的光谱分析的装置和方法,包括以下步骤:选择光束(4)的光谱的某一部分以提供滤波的 检测滤光光束(12)以提供对应于滤光光束(12)的功率振幅(O(λ))的电信号,检测滤光光束(12)以提供 电信号对应于滤波光束(12)的波长,并且提供作为滤波光束(12)的波长的函数的功率振幅。

    Determination of light absorption pathlength in a vertical-beam photometer
    260.
    发明授权
    Determination of light absorption pathlength in a vertical-beam photometer 有权
    垂直光束光度计中光吸收光程的测定

    公开(公告)号:US07663755B2

    公开(公告)日:2010-02-16

    申请号:US12036931

    申请日:2008-02-25

    Abstract: Photometric methods for determining optical pathlength of liquid samples containing analytes dissolved or suspended in a solvent are provided that rely on determining a relationship between the light absorption properties of the solvent and the optical pathlength of liquid samples containing the solvent. This relationship is used to establish the optical pathlength for samples containing an unknown concentration of analyte but having similar solvent composition. Methods are also provided for determining the concentration of analyte in such samples where both the optical pathlength and the concentration of analyte are unknown. The methods may rely on separately determining, at different wavelengths of light, light absorption by the solvent and light absorption by the analyte. Light absorption by the analyte, together with the optical pathlength so determined, is used to calculate the concentration of the analyte.

    Abstract translation: 提供用于确定含有溶解或悬浮在溶剂中的分析物的液体样品的光程长度的光度测定方法,其依赖于确定溶剂的光吸收性质和含有溶剂的液体样品的光程长度之间的关系。 该关系用于建立含有未知浓度的分析物但具有相似溶剂组成的样品的光程。 还提供了用于确定样品中分析物浓度的方法,其中光程长和分析物浓度都是未知的。 这些方法可以依赖于在不同波长的光下分别测定溶剂的光吸收和被分析物的光吸收。 被分析物的光吸收以及如此确定的光程长度被用于计算分析物的浓度。

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