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11.发明授权Cell carrier, associated methods for making cell carrier and culturing cells using the same 有权细胞载体，用于制备细胞载体和使用其的培养细胞的相关方法公开(公告)号：US09534206B2
公开(公告)日：2017-01-03
申请号：US14461860
申请日：2014-08-18
Applicant: General Electric Company
Inventor： Brian Michael Davis , Kenneth Roger Conway , Evelina Roxana Loghin , Andrew Arthur Paul Burns , David Gilles Gascoyne , Scott Michael Miller
CPC classification number: C12N5/0696 , C12N5/0068 , C12N2533/30 , C12N2533/90 , C12N2535/10 , C12N2537/00 , Y10T156/1039
Abstract: A carrier for expansion of induced pluripotent stem cells is provided, wherein the carrier comprises a substrate comprising one or more outer surfaces, wherein the one or more outer surfaces are modified with gas plasma treatment, and one or more structured indentations on one or more of the outer surfaces. The carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.05 mm to 1.2 mm and each of the structured indentations has a major axis in a range from about 0.1 mm to 0.5 mm, a minor axis in a range from about 0.1 mm to 0.5 mm and a depth in a range from about 0.025 mm to about 0.5 mm. A method of making the carrier, and culturing induced pluripotent stem cells using the same carrier are also provided.
Abstract translation: 提供了用于诱导多能干细胞扩增的载体，其中所述载体包括包含一个或多个外表面的基底，其中所述一个或多个外表面用气体等离子体处理进行修饰，以及一个或多个结构化凹陷 外表面。 载体的长度至少约0.2mm，宽度至少约0.2mm，高度在约0.05mm至1.2mm的范围内，并且每个结构化凹陷的长轴在约0.1mm至 0.5mm，短轴在约0.1mm至0.5mm的范围内，深度在约0.025mm至约0.5mm的范围内。 还提供了制造载体的方法，以及使用相同的载体培养诱导的多能干细胞。
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公开(公告)号：US20190009276A1
公开(公告)日：2019-01-10
申请号：US16036430
申请日：2018-07-16
Applicant: GENERAL ELECTRIC COMPANY , University of Washington
Inventor： David Roger Moore , Matthew Jeremiah Misner , Andrew Arthur Paul Burns , Joshua Bishop , Lisa K. Lafleur , Maxwell Wheeler
IPC: B01L7/00 , C12Q1/6844 , B01L3/00 , C12Q1/6806
Abstract: The present disclosure relates to a sample assessment device. By way of example, the sample assessment device may include a substrate including a sample application region; an amplification region comprising a plurality of amplification reagents; a waste region comprising an entrance fluidically coupled to the amplification region and extending away from the amplification region; and a detection region spaced apart from the amplification region. The sample assessment device may also include a valve coupled to the substrate and configured to separate the amplification region from the detection region in a closed configuration, wherein the amplification region and the valve are positioned on the sample assessment device between the sample application region and the detection region and wherein the sample assessment device is configured to permit lateral flow from the amplification region to the detection region when the valve is in an open configuration.
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公开(公告)号：US09926523B2
公开(公告)日：2018-03-27
申请号：US13839409
申请日：2013-03-15
Applicant: General Electric Company
CPC classification number: C12M23/12 , C12N5/0068 , C12N2533/30 , C12N2535/10
Abstract: A carrier for growing stem cells is provided, the carrier comprises a substrate comprising one or more outer surfaces; and a hydrophilic, water soluble coating material disposed and dried on one or more of the outer surfaces. The carrier comprises one or more structured indentations on one or more of the outer surfaces, wherein the carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.05 mm to 1.2 mm and each of the structured indentations has a major axis in a range from about 0.1 mm to 0.5 mm, a minor axis in a range from about 0.1 mm to 0.5 mm and a depth in a range from about 0.025 mm to about 0.5 mm. A method of culturing stem cells and stromal cells using the same carrier are also provided.
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14.发明授权Cell carrier, associated methods for making cell carrier and culturing cells using the same 有权细胞载体，用于制备细胞载体和使用其的培养细胞的相关方法公开(公告)号：US09518249B2
公开(公告)日：2016-12-13
申请号：US13839049
申请日：2013-03-15
Applicant: General Electric Company
Inventor： Brian Michael Davis , Evelina Roxana Loghin , Kenneth Roger Conway , Andrew Arthur Paul Burns , David Gilles Gascoyne
IPC: C12N5/0735 , C12N5/00
CPC classification number: C12N5/0068 , B29C37/0025 , B29C59/02 , B29C71/0081 , B29K2025/06 , B29K2105/256 , B29K2883/00 , B29L2031/712 , C12N2501/999 , C12N2533/30 , C12N2533/52 , C12N2533/54 , C12N2533/90 , C12N2535/10 , C12N2537/00 , C12N2539/10 , Y10T156/1039
Abstract: A carrier for expansion of pluripotent stem cells is provided, wherein the carrier comprises a substrate comprising one or more outer surfaces, wherein the one or more outer surfaces are modified with gas plasma treatment, and one or more structured indentations on one or more of the outer surfaces. The carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.05 mm to 1.2 mm and each of the structured indentations has a major axis in a range from about 0.1 mm to 0.5 mm, a minor axis in a range from about 0.1 mm to 0.5 mm and a depth in a range from about 0.025 mm to about 0.5 mm. A method of making the carrier, and culturing stromal cells using the same carrier are also provided.
Abstract translation: 提供了用于多能干细胞扩增的载体，其中所述载体包括包含一个或多个外表面的基底，其中所述一个或多个外表面用气体等离子体处理进行修饰，以及一个或多个结构化凹陷 外表面。 载体的长度至少约0.2mm，宽度至少约0.2mm，高度在约0.05mm至1.2mm的范围内，并且每个结构化凹陷的长轴在约0.1mm至 0.5mm，短轴在约0.1mm至0.5mm的范围内，深度在约0.025mm至约0.5mm的范围内。 还提供了制造载体和使用相同载体培养基质细胞的方法。
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公开(公告)号：US20160002621A1
公开(公告)日：2016-01-07
申请号：US14321160
申请日：2014-07-01
Applicant: General Electric Company
Inventor： John Richard Nelson , David Roger Moore , Robert Scott Duthie , Matthew Jeremiah Misner , Gregory Andrew Grossmann , Elizabeth Marie Dees , Patrick McCoy Spooner , Erik Leeming Kvam , Andrew Arthur Paul Burns , Vicki Herzl Watkins
IPC: C12N15/10
CPC classification number: C12N15/1006 , C12Q1/6834 , C12Q2565/625
Abstract: A method is provided herein, the method includes: applying a sample comprising target nucleic acids to a sample application zone of a substrate; and flowing a nucleic acid amplification reaction mixture across a length of the substrate through the sample application zone to amplify the target nucleic acid forming a nucleic acid amplification product; wherein the target nucleic acid having a first molecular weight is substantially immobilized at the sample application zone and wherein the amplification product having a second molecular weight migrates away from the sample application zone. An associated device is also provided.
Abstract translation: 本文提供了一种方法，所述方法包括：将包含靶核酸的样品施加到底物的样品施用区; 并使核酸扩增反应混合物通过样品施用区跨越一定长度的底物扩增形成核酸扩增产物的靶核酸; 其中具有第一分子量的靶核酸基本上固定在样品施用区，并且其中具有第二分子量的扩增产物从样品施用区迁移。 还提供了相关联的设备。
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Patent Agency Ranking
16.发明申请CELL CARRIER, ASSOCIATED METHODS FOR MAKING CELL CARRIER AND CULTURING CELLS USING THE SAME 有权细胞载体，用于制备细胞载体和培养细胞的相关方法公开(公告)号：US20140186946A1
公开(公告)日：2014-07-03
申请号：US13839049
申请日：2013-03-15
Applicant: General Electric Company
Inventor： Brian Michael Davis , Evelina Roxana Loghin , Kenneth Roger Conway , Andrew Arthur Paul Burns , David Gilles Gascoyne
IPC: C12N5/0735
CPC classification number: C12N5/0068 , B29C37/0025 , B29C59/02 , B29C71/0081 , B29K2025/06 , B29K2105/256 , B29K2883/00 , B29L2031/712 , C12N2501/999 , C12N2533/30 , C12N2533/52 , C12N2533/54 , C12N2533/90 , C12N2535/10 , C12N2537/00 , C12N2539/10 , Y10T156/1039
Abstract: A carrier for expansion of pluripotent stem cells is provided, wherein the carrier comprises a substrate comprising one or more outer surfaces, wherein the one or more outer surfaces are modified with gas plasma treatment, and one or more structured indentations on one or more of the outer surfaces. The carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.05 mm to 1.2 mm and each of the structured indentations has a major axis in a range from about 0.1 mm to 0.5 mm, a minor axis in a range from about 0.1 mm to 0.5 mm and a depth in a range from about 0.025 mm to about 0.5 mm. A method of making the carrier, and culturing stromal cells using the same carrier are also provided.
Abstract translation: 提供了用于多能干细胞扩增的载体，其中所述载体包括包含一个或多个外表面的基底，其中所述一个或多个外表面用气体等离子体处理进行修饰，以及一个或多个结构化凹陷 外表面。 载体的长度至少约0.2mm，宽度至少约0.2mm，高度在约0.05mm至1.2mm的范围内，并且每个结构化凹陷的长轴在约0.1mm至 0.5mm，短轴在约0.1mm至0.5mm的范围内，深度在约0.025mm至约0.5mm的范围内。 还提供了制造载体和使用相同载体培养基质细胞的方法。
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公开(公告)号：US11173490B2
公开(公告)日：2021-11-16
申请号：US16036430
申请日：2018-07-16
Applicant: GENERAL ELECTRIC COMPANY , University of Washington
Inventor： David Roger Moore , Matthew Jeremiah Misner , Andrew Arthur Paul Burns , Joshua Bishop , Lisa K. Lafleur , Maxwell Wheeler
IPC: B01L7/00 , C12Q1/6806 , C12Q1/6844 , B01L3/00
Abstract: The present disclosure relates to a sample assessment device. By way of example, the sample assessment device may include a substrate including a sample application region; an amplification region comprising a plurality of amplification reagents; a waste region comprising an entrance fluidically coupled to the amplification region and extending away from the amplification region; and a detection region spaced apart from the amplification region. The sample assessment device may also include a valve coupled to the substrate and configured to separate the amplification region from the detection region in a closed configuration, wherein the amplification region and the valve are positioned on the sample assessment device between the sample application region and the detection region and wherein the sample assessment device is configured to permit lateral flow from the amplification region to the detection region when the valve is in an open configuration.
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公开(公告)号：US10472620B2
公开(公告)日：2019-11-12
申请号：US14321160
申请日：2014-07-01
Applicant: General Electric Company
Inventor： John Richard Nelson , David Roger Moore , Robert Scott Duthie , Matthew Jeremiah Misner , Gregory Andrew Grossmann , Elizabeth Marie Dees , Patrick McCoy Spooner , Erik Leeming Kvam , Andrew Arthur Paul Burns , Vicki Herzl Watkins
Abstract: A method is provided herein, the method includes: applying a sample comprising target nucleic acids to a sample application zone of a substrate; and flowing a nucleic acid amplification reaction mixture across a length of the substrate through the sample application zone to amplify the target nucleic acid forming a nucleic acid amplification product; wherein the target nucleic acid having a first molecular weight is substantially immobilized at the sample application zone and wherein the amplification product having a second molecular weight migrates away from the sample application zone. An associated device is also provided.
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公开(公告)号：US10040069B2
公开(公告)日：2018-08-07
申请号：US14807497
申请日：2015-07-23
Applicant: GENERAL ELECTRIC COMPANY , University of Washington
Inventor： David Roger Moore , Matthew Jeremiah Misner , Andrew Arthur Paul Burns , Joshua Bishop , Lisa K. Lafleur , Maxwell Wheeler
IPC: C12Q1/68 , C12P19/34 , B01L7/00 , C12Q1/6806 , C12Q1/6844 , B01L3/00
Abstract: The present disclosure relates to a sample assessment device. By way of example, the sample assessment device may include a substrate including a sample application region; an amplification region comprising a plurality of amplification reagents; a waste region comprising an entrance fluidically coupled to the amplification region and extending away from the amplification region; and a detection region spaced apart from the amplification region. The sample assessment device may also include a valve coupled to the substrate and configured to separate the amplification region from the detection region in a closed configuration, wherein the amplification region and the valve are positioned on the sample assessment device between the sample application region and the detection region and wherein the sample assessment device is configured to permit lateral flow from the amplification region to the detection region when the valve is in an open configuration.
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20.发明授权公开(公告)号：US09957478B2
公开(公告)日：2018-05-01
申请号：US15345418
申请日：2016-11-07
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Brian Michael Davis , Evelina Roxana Loghin , Kenneth Roger Conway , Andrew Arthur Paul Burns , David Gilles Gascoyne
CPC classification number: C12N5/0068 , B29C37/0025 , B29C59/02 , B29C71/0081 , B29K2025/06 , B29K2105/256 , B29K2883/00 , B29L2031/712 , C12N2501/999 , C12N2533/30 , C12N2533/52 , C12N2533/54 , C12N2533/90 , C12N2535/10 , C12N2537/00 , C12N2539/10 , Y10T156/1039
Abstract: A carrier for expansion of pluripotent stem cells is provided, wherein the carrier comprises a substrate comprising one or more outer surfaces, wherein the one or more outer surfaces are modified with gas plasma treatment, and one or more structured indentations on one or more of the outer surfaces. The carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.05 mm to 1.2 mm and each of the structured indentations has a major axis in a range from about 0.1 mm to 0.5 mm, a minor axis in a range from about 0.1 mm to 0.5 mm and a depth in a range from about 0.025 mm to about 0.5 mm. A method of making the carrier, and culturing stromal cells using the same carrier are also provided.
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