公开(公告)号：US20160186244A1

公开(公告)日：2016-06-30

申请号：US15068364

申请日：2016-03-11

Applicant: Exact Sciences Corporation

Inventor： Rebecca Oldham-Haltom ,  Hongzhi Zou ,  Graham P. Lidgard ,  Michael J. Domanico ,  Hatim Allawi

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q1/6818 ,  C12Q1/686 ,  C12Q1/6886 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2525/301 ,  C12Q2527/101 ,  C12Q2561/109 ,  C12Q2565/1015

Abstract: A cleavage-based real-time PCR assay method is provided. In general terms, the assay method includes subjecting a reaction mixture comprising a) PCR reagents for amplifying a nucleic acid target, and b) flap cleavage reagents for performing a flap cleavage assay on the amplified nucleic acid target to two sets of thermocycling conditions. No additional reagents are added to the reaction between said first and second sets of cycles and, in each cycle of the second set of cycles, cleavage of a flap probe is measured.

公开(公告)号：US20160060683A1

公开(公告)日：2016-03-03

申请号：US14939651

申请日：2015-11-12

Applicant: Exact Sciences Corporation

Inventor： Hatim Allawi ,  Rebecca Oldham-Haltom ,  Zubin Gagrat ,  Michael Domanico ,  Graham Lidgard

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6851 ,  C12Q2521/101 ,  C12Q2527/125 ,  C12Q2527/137 ,  C12Q2549/125

Abstract: Provided herein is technology relating to the amplification-based detection of nucleic acids and particularly, but not exclusively, to methods and compositions for minimizing variability in the activity between different samples or manufacturing lots of DNA polymerases, such as Taq DNA polymerase.

Abstract translation: 本文提供了与核酸的基于扩增的检测有关的技术，特别但并非排他地涉及用于最小化不同样品或制造大量DNA聚合酶如Taq DNA聚合酶之间的活性的变异性的方法和组合物。

公开(公告)号：US20230183782A1

公开(公告)日：2023-06-15

申请号：US18045927

申请日：2022-10-12

Applicant: Exact Sciences Corporation

Inventor： Rebecca Oldham-Haltom ,  Hongzhi Zou ,  Graham P. Lidgard ,  Michael J. Domanico ,  Hatim Allawi

IPC: C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6818 ,  C12Q1/6886

CPC classification number: C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6818 ,  C12Q1/6886 ,  C12Q2600/156 ,  C12Q2600/158

Abstract: A cleavage-based real-time PCR assay method is provided. In general terms, the assay method includes subjecting a reaction mixture comprising a) PCR reagents for amplifying a nucleic acid target, and b) flap cleavage reagents for performing a flap cleavage assay on the amplified nucleic acid target to two sets of thermocycling conditions. No additional reagents are added to the reaction between said first and second sets of cycles and, in each cycle of the second set of cycles, cleavage of a flap probe is measured.

公开(公告)号：US09783856B2

公开(公告)日：2017-10-10

申请号：US14811266

申请日：2015-07-28

Applicant: Exact Sciences Corporation

Inventor： Rebecca Oldham-Haltom ,  Hatim Allawi ,  Hongzhi Zou ,  Michael J. Domanico ,  Graham P. Lidgard

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q1/6858 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2525/161 ,  C12Q2561/109 ,  C12Q2565/1015

Abstract: Provided herein is reagent mixture comprising multiplexed amplification reagents and flap assay reagents for detecting, in a single reaction, mutant copies of the KRAS gene that contain any of the 34A, 34C, 34T, 35A, 35C, 35T or 38A point mutations. Methods that employ the reagent mix and kits for performing the same are also provided.

公开(公告)号：US09212392B2

公开(公告)日：2015-12-15

申请号：US14036649

申请日：2013-09-25

Applicant: Exact Sciences Corporation

Inventor： Hatim Allawi ,  Rebecca Oldham-Haltom ,  Zubin Gagrat ,  Michael Domanico ,  Graham Lidgard

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/686 ,  C12Q1/6851 ,  C12Q2521/101 ,  C12Q2527/125 ,  C12Q2527/137 ,  C12Q2549/125

Abstract: Provided herein is technology relating to the amplification-based detection of nucleic acids and particularly, but not exclusively, to methods and compositions for minimizing variability in the activity between different samples or manufacturing lots of DNA polymerases, such as Taq DNA polymerase.

Abstract translation: 本文提供了与核酸的基于扩增的检测有关的技术，特别但并非排他地涉及用于最小化不同样品之间的活性或制造大量DNA聚合酶如Taq DNA聚合酶的变异性的方法和组合物。

公开(公告)号：US20130143216A1

公开(公告)日：2013-06-06

申请号：US13720757

申请日：2012-12-19

Applicant: EXACT SCIENCES CORPORATION

Inventor： Rebecca Oldham-Haltom ,  Hongzhi Zou ,  Graham P. Lidgard ,  Michael J. Domanico ,  Hatim Allawi

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q1/6818 ,  C12Q1/686 ,  C12Q1/6886 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2525/301 ,  C12Q2527/101 ,  C12Q2561/109 ,  C12Q2565/1015

Abstract: A cleavage-based real-time PCR assay method is provided. In general terms, the assay method includes subjecting a reaction mixture comprising a) PCR reagents for amplifying a nucleic acid target, and b) flap cleavage reagents for performing a flap cleavage assay on the amplified nucleic acid target to two sets of thermocycling conditions. No additional reagents are added to the reaction between said first and second sets of cycles and, in each cycle of the second set of cycles, cleavage of a flap probe is measured.

Abstract translation: 提供基于切割的实时PCR测定方法。 一般而言，测定方法包括对包含a）PCR试剂的反应混合物进行核酸靶扩增，以及b）用于在扩增的核酸靶上进行皮瓣裂解测定的折叠裂解试剂至两组热循环条件。 在所述第一组和第二组循环之间没有向所述反应中加入另外的试剂，并且在第二组循环的每个循环中，测量瓣探针的切割。