公开(公告)号：US20180179489A1

公开(公告)日：2018-06-28

申请号：US15579739

申请日：2016-06-08

Applicant: Corning Incorporated

Inventor： Stephen Joseph Caracci ,  David Henry ,  Corinne Walerack ,  Yue Zhou

IPC: C12N5/00 ,  C12N5/0775 ,  C12N5/071

CPC classification number: C12N5/0075 ,  C08L5/00 ,  C12N5/0663 ,  C12N5/0686 ,  C12N5/0688 ,  C12N2531/00 ,  C12N2533/18 ,  C12N2533/70

Abstract: A cell culture article is provided. The cell culture substrate includes a polygalacturonic acid compound selected from at least one of: pectic acid or salts thereof, and partially esterified pectic acid having a degree of esterification from 1 to 40 mol % or salts thereof. The polygalacturonic acid compound is crosslinked with a divalent cation and the divalent cation concentration ranges from 0.5 to 2 g/1 of the substrate.

公开(公告)号：US20160145600A1

公开(公告)日：2016-05-26

申请号：US14950771

申请日：2015-11-24

Applicant: Corning Incorporated

Inventor： Stephen Joseph Caracci ,  Ye Fang ,  Ann MeeJin Ferrie ,  Yan Jin ,  Lingyan Wang ,  Yue Zhou

IPC: C12N13/00 ,  H01F1/147 ,  H01F1/20 ,  C12N5/00 ,  G01N1/40

CPC classification number: C12N13/00 ,  C12N1/02 ,  H01F1/26 ,  H01F1/37

Abstract: Magnetic microcarrier beads have a particle size of 1 to 1000 micrometers and include a composite core and a polymer coating that surrounds and encapsulates the core. The composite core includes magnetic particles embedded within an indigestible polymer matrix. The coating is a digestible or indigestible polymer that facilitates cell adhesion to the surface of the beads during cell culture. Magnetic force can be used to agitate the microcarrier beads during cell culture as well as to separate the beads from cultured cells or processed bio-media.

Abstract translation: 磁性微载体珠具有1至1000微米的粒度，并且包括复合芯和围绕并封装芯的聚合物涂层。 复合芯包括嵌入在难消化的聚合物基质内的磁性颗粒。 该涂层是可消化或消化不良的聚合物，其在细胞培养期间促进细胞与珠表面的粘附。 可以使用磁力在细胞培养过程中搅动微载体珠，以及将珠与培养的细胞或加工的生物培养基分离。

公开(公告)号：US20150064718A1

公开(公告)日：2015-03-05

申请号：US14471060

申请日：2014-08-28

Applicant: Corning Incorporated

Inventor： Stephen Joseph Caracci ,  Martin Joseph Murtagh ,  Christopher Bankole Shogbon ,  Yue Zhou

IPC: G01N33/543 ,  B29C47/00 ,  B29C47/40 ,  H01F1/01

CPC classification number: G01N33/5434 ,  B29B7/46 ,  B29B7/726 ,  B29B9/06 ,  B29C48/001 ,  B29C48/0022 ,  B29C48/022 ,  B29C48/04 ,  B29C48/40 ,  B29C48/402 ,  B29C2793/009 ,  B29K2023/14 ,  B29K2025/08 ,  B29K2035/00 ,  B29K2105/0085 ,  B29K2105/16 ,  B29K2503/06 ,  B29K2505/00 ,  B29K2505/12 ,  B29K2995/0008 ,  B29K2995/007 ,  B29K2995/0077 ,  B29K2995/0094 ,  C08K3/22 ,  C08K2003/2265 ,  C08K2003/2275 ,  C08K2201/003 ,  H01F1/01 ,  Y10T428/2982 ,  C08L51/06 ,  C08L25/08

Abstract: An organic/inorganic composite is provided. The organic/inorganic composite materials are sufficiently hard and brittle to be ground to form particles suitable for biological or chemical separation applications. The organic/inorganic composite materials can be magnetic or magnetically susceptible and can have functional reactive groups to allow attachment of biomolecules. Methods of making and using the organic/inorganic composites are also provided.

Abstract translation: 提供有机/无机复合材料。 有机/无机复合材料足够硬且易碎以研磨以形成适于生物或化学分离应用的颗粒。 有机/无机复合材料可以是磁性或磁性敏感的，并且可以具有功能反应性基团以允许附着生物分子。 还提供了制备和使用有机/无机复合材料的方法。

公开(公告)号：US20150010999A1

公开(公告)日：2015-01-08

申请号：US14375614

申请日：2013-01-31

Applicant: CORNING INCORPORATED

Inventor： Stephen Joseph Caracci ,  David Henry ,  Jessica Jo Kelley ,  Mark Alan Lewis ,  Yue Zhou

IPC: C12N5/00 ,  C12N5/0735 ,  B05D3/02 ,  C12N5/0775

CPC classification number: C12N5/0068 ,  B05D3/0254 ,  C12M23/20 ,  C12N5/0037 ,  C12N5/0056 ,  C12N5/0606 ,  C12N5/0663 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2533/30 ,  C12N2533/40 ,  C12N2533/50

Abstract: An aqueous cell culture medium composition includes an aqueous cell culture solution configured to support the culture of mammalian cells. The composition further includes a synthetic polymer conjugated to a polypeptide dissolved in the aqueous cell culture solution. The synthetic polymer conjugated to a polypeptide is configured to attach to the surface of a cell culture article under cell culture conditions. Incubation of the aqueous cell culture medium composition on a cell culture surface under cell culture conditions results is attachment to the surface of the synthetic polymer conjugated to the polypeptide.

Abstract translation: 水性细胞培养基组合物包括配置成支持哺乳动物细胞培养的水性细胞培养液。 该组合物还包括与溶解在水性细胞培养溶液中的多肽缀合的合成聚合物。 与多肽缀合的合成聚合物被配置为在细胞培养条件下附着于细胞培养物品的表面。 水细胞培养基组合物在细胞培养条件下在细胞培养物表面上的孵育结果是与多肽缀合的合成聚合物表面的附着。