公开(公告)号：US10767146B2

公开(公告)日：2020-09-08

申请号：US15031958

申请日：2014-10-24

Applicant: Becton, Dickinson and Company

Inventor： Mei Yang-Woytowitz ,  Brent Pohl ,  Gary F. Hershner ,  Eric Ursprung ,  Michael A. Brasch ,  Ming-hsiung Yeh ,  Patrick Shawn Beaty ,  Charles C. Yu ,  Timothy M. Wiles ,  Liping Feng ,  Ben Turng ,  Xiaofei Chang ,  Patrick R. Murray

IPC: C12Q1/02 ,  C12M1/00 ,  A61J1/20 ,  C12M1/24 ,  A61J1/05 ,  A61J1/14 ,  C12C1/02 ,  B01L3/00

Abstract: An apparatus and associated methods of use for a controlled combination of reagents is disclosed. The apparatus includes a vessel 400, a vessel insert 220, and a cap element 200. The vessel 400 has a body portion 410 for receiving a biological sample. The vessel insert 220 receives at least one reagent therein. Preferably, the vessel insert 220 is received in a portion 420 of the vessel 400. The cap element 200 is attached to the vessel 400 to secure the vessel insert 220 in the vessel 400. During use, the vessel insert 220 is adapted to release its contents when the biological sample is introduced into the body portion 410 of the vessel 400 upon application of an intermixing force to the vessel insert 220. A variety of intermixing forces may be applied, depending upon the embodiment of the present invention and its associated methods of use.

公开(公告)号：US12031118B2

公开(公告)日：2024-07-09

申请号：US17019802

申请日：2020-09-14

Applicant: BECTON DICKINSON AND COMPANY

Inventor： Patrick Shawn Beaty ,  Michael A. Brasch ,  Suneil Hosmane ,  David Polley ,  Julie L Rosales ,  Kerry Lynn Smith

IPC: C12M1/34 ,  C12Q1/02 ,  C12Q1/04 ,  G01N27/02 ,  G01N33/487 ,  G01N33/49

CPC classification number: C12M41/36 ,  C12Q1/02 ,  C12Q1/04 ,  G01N27/02 ,  G01N33/48785 ,  G01N27/026 ,  G01N33/49

Abstract: A system and method with increased sensitivity to microorganism growth. The system includes signal processing electronic circuit connected to a consumable or vessel through two or more electrodes that fully penetrate the vessel and are in contact with the fluid contents. The electronic circuit is configured to detect a component of the total impedance of the sample, specifically the “out-of-phase” or imaginary reactance component, which has a sensitive response to organism growth in a frequency-dependent manner. The system detects changes in both the composition of charged molecules in the liquid matrix and the number of microorganisms based on monitoring the sample for change in this parameter. This results in a 5-70% reduction in time-to-detection (TTD).
The system and method detect organisms in a plurality of vessel shapes, volumes, and matrix (or media) formats. The electrodes are fully immersed in a continuous body of liquid sample. The distance between electrodes may be adjusted or tuned to fit the needs of the vessel. The voltage inputs can also be adjusted to allow proper detection of the contents within the vessel.

公开(公告)号：US11727699B2

公开(公告)日：2023-08-15

申请号：US16753667

申请日：2018-10-04

Applicant: BECTON DICKINSON AND COMPANY

Inventor： Michael A. Brasch ,  Curtis M. Gosnell ,  Timothy M. Wiles ,  Raphael Rodolphe Marcelpoil ,  Bas Nieuwenhuis ,  Trienko Marten Van Der Kaap ,  Michael Bois

IPC: G06K9/00 ,  G06V20/69 ,  G06F16/58 ,  C12M1/34 ,  G06T7/00 ,  G06N7/01

CPC classification number: G06V20/698 ,  C12M1/3446 ,  C12M41/36 ,  G06F16/5866 ,  G06N7/01 ,  G06T7/0016 ,  G06T2207/10056 ,  G06T2207/30024 ,  G06V2201/03

Abstract: A system and method for developing applications (Apps) for automated assessment and analysis of processed biological samples. Such samples are obtained, combined with nutrient media and incubated. The incubated samples are imaged and the image information is classified according to predetermined criteria. The classified image information is then evaluated according to Apps derived from classified historical image information in a data base. The classified historical image information is compared with the classified image information to provide guidance on further processing of the biological sample through Apps tailored to process provide sample process guidance tailored to the classifications assigned to the image information.

公开(公告)号：US11110416B2

公开(公告)日：2021-09-07

申请号：US15564364

申请日：2016-04-08

Applicant: BECTON DICKINSON AND COMPANY

Inventor： Timothy Mark Wiles ,  Dwight Livingston ,  Jennifer Singelyn ,  William Alan Fox ,  Brian Reuben Langhoff ,  Vikram Patel ,  Sean Tucker ,  Ming-hsiung Yeh ,  Michael A. Brasch

IPC: B01F13/08 ,  B01L3/00 ,  G01N23/225 ,  G01N1/02 ,  G01N35/10 ,  B01L3/02 ,  A61B10/02

Abstract: A device for collecting a biological sample from a semi-solid surface. The device has a shaft with a proximate end and a distal end and a tip integrated with the shaft at the proximate end. The tip has a surface adapted to collect microorganisms thereon or release microorganism from, or both, wherein the adapted surface comprises at least one feature of a recess or extension to increase surface area of the tip and collect microorganisms thereon. Examples of such features include microfeatures with dimensions of about 1000 μm or less. Other examples include a pipette tip.

公开(公告)号：US20200242329A1

公开(公告)日：2020-07-30

申请号：US16753667

申请日：2018-10-04

Applicant: BECTON DICKINSON AND COMPANY

Inventor： Michael A. Brasch ,  Curtis M. Gosnell ,  Timothy M. Wiles ,  Raphael Rodolphe Marcelpoil ,  Bas Nieuwenhuis ,  Trienko Marten Van Der Kaap ,  Michael Bois

IPC: G06K9/00 ,  C12M1/34 ,  G06F16/58 ,  G06T7/00 ,  G06N7/00

Abstract: A system and method for developing applications (Apps) for automated assessment and analysis of processed biological samples. Such samples are obtained, combined with nutrient media and incubated. The incubated samples are imaged and the image information is classified according to predetermined criteria. The classified image information is then evaluated according to Apps derived from classified historical image information in a data base. The classified historical image information is compared with the classified image information to provide guidance on further processing of the biological sample through Apps tailored to process provide sample process guidance tailored to the classifications assigned to the image information.

公开(公告)号：US20170240855A1

公开(公告)日：2017-08-24

申请号：US15518989

申请日：2015-10-14

Applicant: Becton, Dickinson and Company

Inventor： Patrick Shawn Beaty ,  Michael A. Brasch ,  Suneil Hosmane ,  David Polley ,  Julie L. Rosales ,  Kerry Lynn Smith

IPC: C12M1/34 ,  G01N27/02 ,  G01N33/487 ,  C12Q1/02

CPC classification number: C12M41/36 ,  C12Q1/02 ,  C12Q1/04 ,  G01N27/02 ,  G01N27/026 ,  G01N33/48785 ,  G01N33/49

Abstract: A system and method with increased sensitivity to microorganism growth. The system includes signal processing electronic circuit connected to a consumable or vessel through two or more electrodes that fully penetrate the vessel and are in contact with the fluid contents. The electronic circuit is configured to detect a component of the total impedance of the sample, specifically the “out-of-phase” or imaginary reactance component, which has a sensitive response to organism growth in a frequency-dependent manner. The system detects changes in both the composition of charged molecules in the liquid matrix and the number of microorganisms based on monitoring the sample for change in this parameter. This results in a 5-70% reduction in time-to-detection (TTD). The system and method detect organisms in a plurality of vessel shapes, volumes, and matrix (or media) formats. The electrodes are fully immersed in a continuous body of liquid sample. The distance between electrodes may be adjusted or tuned to fit the needs of the vessel. The voltage inputs can also be adjusted to allow proper detection of the contents within the vessel.

公开(公告)号：US09671364B2

公开(公告)日：2017-06-06

申请号：US14367549

申请日：2012-12-19

Applicant: Becton, Dickinson and Company

Inventor： Song Shi ,  James G. Nadeau ,  Michael A. Brasch

IPC: C12Q1/00 ,  C12Q1/04 ,  C12Q1/06 ,  C12N13/00 ,  G01N27/414 ,  B01L3/00 ,  G01N33/487

CPC classification number: G01N27/4145 ,  B01L3/5021 ,  B01L2300/0636 ,  B01L2300/0851 ,  B01L2300/0893 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  C12Q1/04 ,  G01N33/48735 ,  G01N2800/26

Abstract: An array of micro-chambers (220) with individual ion sensitive field effect transistors (ISFETs) (300) disposed therein for monitoring single cell activity in the microarray to determine the presence or absence of microorganisms in a sample (390). In addition to the presence or absence of a single cell, certain further embodiments contemplate monitoring cell behavior. Cell behavior includes the entire range of cell activity as well as cell response to changes in environmental conditions of changes in response due to the addition of sample constituents.