公开(公告)号：US20140370509A1

公开(公告)日：2014-12-18

申请号：US14352031

申请日：2012-10-16

Applicant: Eppendorf AG

Inventor： Michael Wild ,  Christoph Jolie

IPC: G01N21/64

CPC classification number: G01N21/6486 ,  G01N21/274 ,  G01N21/64 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2201/062 ,  G01N2201/124 ,  G01N2201/127

Abstract: The invention is related to a method for the quantitative optical measurement of a characteristic property of at least one analyte in at least one laboratory sample, in particular for the fluorescence measurement of at least one biochemical or biological sample, has the method using a laboratory apparatus, which h at least one light source and at least one detector device, the apparatus utilizing at least sensitivity parameter S, which controls the capability of the laboratory apparatus to detect a signal by means of the at least one detector device, the method using source light for causing the at least one sample to emit a sample light, and the at least one detector device for detecting sample light and utilizing the at least one sensitivity parameter S to detect the corresponding at least one intensity I of the sample light, the method comprising the steps: —determining at least one reference point (S_ref; I ref); —using at least one first sensitivity parameter S_m1, which is not the same as S_ref, for measuring at least one first intensity I_m1 of sample light as-signed to a first analyte; —determining a quantity Q1, which is a measure for the slope of a line, which is determined by utilizing the at least one reference point (S_ref; 1_ref) and the at least one measurement point (S_m1; I_m1); using the quantity Q1 for calculating a first analyte value C_m1, which is dependent on Q1 and which is characteristic for a property of the first analyte, in particular for a concentration of the first analyte in the at least one sample, in particular according to the formula Q1=(I_m1−1_ref)/(S_m1−S_ref). The method, further, is related to a laboratory apparatus, which is configured to apply the method according to the invention.

Abstract translation: 本发明涉及一种用于定量光学测量至少一个实验室样品中至少一种分析物的特征性质的方法，特别是用于至少一种生化或生物样品的荧光测量的方法，具有使用实验室装置的方法 ，其至少一个光源和至少一个检测器装置，所述装置至少使用灵敏度参数S，其控制实验室装置通过至少一个检测器装置检测信号的能力，所述方法使用源 用于使所述至少一个样品发射样品光的光，以及所述至少一个检测器装置，用于检测样品光并利用所述至少一个灵敏度参数S来检测所述样品光的相应的至少一个强度I，所述方法 包括以下步骤： - 确定至少一个参考点（S_ref; I ref）; - 使用与S_ref不同的至少一个第一灵敏度参数S_m1来测量与第一分析物相符合的样本光的至少一个第一强度I_m1; - 确定通过利用所述至少一个参考点（S_ref; 1_ref）和所述至少一个测量点（S_m1; I_m1）确定的线的斜率的度量的量Q1; 使用数量Q1来计算取决于Q1的第一分析物值C_m1，其特征在于至少一个样品中的第一分析物的浓度，特别是对于至少一个样品中的第一分析物的浓度，其特征在于第一分析物的性质， 公式Q1 =（I_m1-1_ref）/（S_m1-S_ref）。 该方法还涉及实验室设备，其被配置为应用根据本发明的方法。

公开(公告)号：US20130224087A1

公开(公告)日：2013-08-29

申请号：US13644254

申请日：2012-10-03

Applicant: Eppendorf AG

Inventor： Burkhardt Reichmuth

IPC: B01L3/02

CPC classification number: B01L3/021 ,  B01L3/0217 ,  B01L3/0234 ,  B01L2200/12 ,  B01L2300/12

Abstract: A manual dosing device comprising an elongated frame as a support structure, means for releasably holding a syringe or pipette tip at the bottom end of the frame, drive means that are attached above the means for releasably holding to the frame, displacement means for displacing a fluid in the syringe or pipette tip that can be driven by the drive means and are connected to the means for releasably holding, and a housing encasing the frame.

Abstract translation: 一种手动计量装置，其包括作为支撑结构的细长框架，用于在所述框架的底端可释放地保持注射器或移液管末端的装置，附接在所述装置上方的驱动装置，用于可释放地保持在所述框架上;移位装置， 注射器或移液器吸头中的流体可以由驱动装置驱动并且连接到用于可释放地保持的装置和包围框架的壳体。

公开(公告)号：US20030150982A1

公开(公告)日：2003-08-14

申请号：US10355319

申请日：2003-01-31

Applicant: EPPENDORF AG

Inventor： Hanna Rethwisch ,  Jorg Richter ,  Boris von Beichmann ,  Jurgen Lohn

IPC: H01J005/02

CPC classification number: B01L3/50853 ,  B01L3/50851 ,  B01L2200/0689 ,  B01L2300/042 ,  B01L2300/0829

Abstract: A covering for apertures of reaction receptacles formed in a microtitration plate, including a hermetic, elastically deforming mat or foil, a frame, and connecting elements. The mat or foil, upon planar deposition on the microtitration plate and following compression, seals the apertures. The connecting elements are fitted onto at least one of the mat or foil and frame and detachably secure the mat or foil to the frame while creating a tension in the mat or foil implementing the connection. The frame (11, 110) is dimensioned so as to permit the frame to be deposited on the microtitration plate (13), such-that the mat (12, 120) or foil rests in a planar manner on the microtitration plate, and such that the frame is accessible from above to a pressure and/or heat applying device.

Abstract translation: 形成在微量滴定板中的反应容器孔的覆盖物，包括密封的，弹性变形的垫或箔，框架和连接元件。 在平板沉积在微量滴定板上并在压缩之后，垫或箔密封孔。 连接元件安装在垫或箔和框架中的至少一个上，并且将垫或箔可拆卸地固定到框架上，同时在实现连接的垫或箔中产生张力。 框架（11,110）的尺寸被设计成允许框架沉积在微量滴定板（13）上，使得垫子（12,120）或箔片以平面方式搁置在微量滴定板上， 框架可从上方接近压力和/或加热装置。

公开(公告)号：US20030092553A1

公开(公告)日：2003-05-15

申请号：US10289937

申请日：2002-11-07

Applicant: Eppendorf AG

Inventor： Werner Lurz ,  Roland Lippoldt

IPC: B04B005/02

CPC classification number: B04B5/0421

Abstract: A laboratory centrifuge comprising at least one bucket receiving sample liquids and mounted in swing-out manner on a rotationally driven rotor and further comprising a noise abatement system to reduce the bucket-generated noise is characterized in that said system consists of at least one turbulence generated mounted on the external surface of the bucket.

Abstract translation: 一种实验室离心机，其包括至少一个容纳样品液体并且以旋转方式安装在旋转驱动的转子上并且还包括减少铲斗产生的噪声的噪声消除系统的桶，其特征在于，所述系统由至少一个产生的湍流 安装在桶的外表面上。

公开(公告)号：US20030092135A1

公开(公告)日：2003-05-15

申请号：US09993064

申请日：2001-11-13

Applicant: Eppendorf AG

Inventor： Lars-Erik Peters

IPC: C12P019/34 ,  C12N009/22

CPC classification number: C12N15/72 ,  C12N9/1252 ,  C12Q1/6848 ,  C12Q2527/127 ,  C12Q2527/125 ,  C12Q2527/101

Abstract: Methods for improving sensitivity and specificity of polynucleotide synthesis are disclosed. The method includes reversibly blocking thermophilic polymerase activity with non-nucleic acid polyanions in a temperature dependent manner. The methods control target specific primer extension throughout all stages of a DNA or RNA amplification reaction. Corresponding compositions and kits are disclosed.

公开(公告)号：US12269027B2

公开(公告)日：2025-04-08

申请号：US17327992

申请日：2021-05-24

Applicant: Eppendorf AG

Inventor： Florian Tesch ,  Peter Molitor

IPC: B01L3/02

Abstract: A pipette with an adjustable volume comprises a housing comprising an outside surface, at least one seat defining an opening, and a displacement apparatus defining a displacement chamber comprising a displacement element configured to be displaced therein. A stroke rod is coupled to the displacement element and configured to be displaced relative to the housing order to displace the displacement element. An adjusting apparatus is configured to adjust a position of an upper stop in the housing, a display apparatus is configured to display a set dosing volume, and a coupling apparatus is positioned between the adjusting apparatus and the display apparatus. The coupling apparatus is configured to couple the adjusting apparatus to the display apparatus in an engaged state and configured to uncouple the adjusting apparatus from the display apparatus in a disengaged state.

公开(公告)号：US12194455B2

公开(公告)日：2025-01-14

申请号：US17058923

申请日：2019-02-21

Applicant: Eppendorf AG

Inventor： Hanna Rethwisch

IPC: B01L3/02

Abstract: A pipette tip comprises a tubular body including an inner circumference and an outer circumference. The tubular body comprises a bottom opening defined at a bottom end of the tubular body and a top opening defined at a top end and configured to clamp onto an attachment of a pipetting device. A seat region is positioned proximate the top opening on the inner circumference and configured to engage an attachment of a pipetting device. At least one groove is defined on the outer circumference. The tubular body is configured to plastically expanded in a radial direction within the at least one groove upon mounting the seat region on the attachment. The attachment comprises a larger diameter than at least part of the seat region to form an interference fit between the attachment and the at least part of the seat region when the pipette tip is clamped onto the attachment.

公开(公告)号：US11980891B2

公开(公告)日：2024-05-14

申请号：US17722801

申请日：2022-04-18

Applicant: Eppendorf AG

Inventor： Jens Wilmer

IPC: B01L3/02 ,  B01L9/00 ,  G01N35/10

CPC classification number: B01L9/543 ,  B01L3/0279 ,  G01N35/1016 ,  G01N35/1074 ,  G01N35/109 ,  B01L3/0227 ,  B01L2200/023 ,  B01L2200/0605 ,  B01L2200/0689 ,  B01L2300/0829

Abstract: A metering head for a metering machine having a carrier having parallel attachments for picking up pipette tips wherein each attachment has a tube having a supporting protrusion at the bottom end at least one sleeve which surrounds the tube and can be axially shifted on the tube, and at least one elastomer O-ring which surrounds the tube a pressure plate above the sleeves having a plurality of first holes through which the tubes extend, wherein the pressure plate can be shifted along the tubes from a release position into a clamping position where the sleeves are pressed, at the bottom ends, against the adjacent O-rings, and the O-rings are expanded to clamp pipette tips; and a first shifting apparatus connected to the pressure plate moving the pressure plate between the release position and clamping position.

公开(公告)号：US11951471B2

公开(公告)日：2024-04-09

申请号：US17354193

申请日：2021-06-22

Applicant: Eppendorf AG

Inventor： Marc Wilth ,  Dana Tuchscherer ,  Matthias Kunsch ,  Florian Tesch ,  Burghardt Reichmuth

IPC: B01L3/02 ,  G01N35/10

CPC classification number: B01L3/0227 ,  B01L3/0282 ,  G01N35/1011 ,  B01L2200/0605 ,  B01L2200/087 ,  B01L2400/0478

Abstract: A pipette for use with a pipette tip or syringe including a piston positioned within a cylinder comprises a pipette housing extending between an upper end and a lower end. Two parallel rods are positioned in the pipette housing, wherein one of the two parallel rods is coupled to a lifting rod. An operating element is configured to protrude outwardly from the pipette housing and be displaced relative to the pipette housing. A gear mechanism is arranged in the pipette housing and comprises a drive comprising a moveable input member and configured to be driven by the operating element, and an output comprising at least one moveable output member and configured to drive the two parallel rods. Successive displacements of the input member by the operating element are configured to alternately displace one of the two parallel rods and then another of the two parallel rods by the output member.

公开(公告)号：US20230110524A1

公开(公告)日：2023-04-13

申请号：US16974160

申请日：2019-04-23

Applicant: Eppendorf AG

Inventor： Burkhardt Reichmuth ,  Uwe Dunker ,  Jens Wilmer ,  Tobias David

IPC: B01L3/02

Abstract: A pipette comprises a pipette housing, a neck configured to clamp on a pipette tip, and a drive apparatus. The drive apparatus comprises a drive element configured to displace a displacement element to draw a liquid sample into the pipette tip and to eject the liquid sample from the pipette tip. A scanning apparatus comprises a scanning element positioned proximate the neck and configured to be displaced relative to the pipette housing by a collar of the pipette tip when clamping the pipette tip onto the neck. A mechanical display apparatus is coupled to the scanning apparatus and comprises a display. When the pipette tip is clamped to the neck the scanning apparatus is configured to adjust the display depending on a height of the collar of the pipette tip relative to the neck, and the neck is configured to accommodate different collar heights.