公开(公告)号：US08672538B2

公开(公告)日：2014-03-18

申请号：US13657599

申请日：2012-10-22

Applicant: Eppendorf AG

Inventor： Lutz Timmann ,  Vinh Duong ,  Stefan Roth ,  Thomas Uschkureit ,  Jurgen Koeppel ,  Thomas Buck ,  Michael Wild

IPC: G01N25/00

CPC classification number: G01N25/00 ,  B01L7/52 ,  B01L2200/147 ,  B01L2200/148 ,  B01L2300/0627 ,  B01L2300/1822 ,  B01L2300/1827

Abstract: The invention relates to a tempering apparatus for tempering a sample comprising: at least one tempering block configured for receiving at least one sample, at least one tempering device arranged for tempering the tempering block, at least one temperature measurement device assigned to the tempering device, at least one control loop to which the tempering device and temperature measurement device are assigned to, at least one control device configured for control of the tempering of the tempering block, wherein the tempering apparatus comprises at least two temperature measurement devices assigned to the control loop, and that to the tempering apparatus a testing device for performing a test method is assigned to, wherein the testing device comprises a signal-connection to at least one of the at least two temperature measurement devices, such that at least one testing quantity of the tempering apparatus is detectable, which characterizes the operational status of the tempering apparatus.

Abstract translation: 本发明涉及一种用于回火样品的回火设备，包括：至少一个回火块，其构造成用于接收至少一个样品，至少一个回火装置，其布置成回火回火块，至少一个温度测量装置分配给回火装置， 至少一个控制回路，回火装置和温度测量装置被分配给至少一个控制装置，至少一个控制装置，其配置为控制回火块的回火，其中回火装置包括分配给控制回路的至少两个温度测量装置 并且对于回火装置，分配用于执行测试方法的测试装置，其中测试装置包括到至少两个温度测量装置中的至少一个的信号连接，使得至少一个测试量 回火装置是可检测的，其特征在于回火装置的操作状态 s。

公开(公告)号：US20140370509A1

公开(公告)日：2014-12-18

申请号：US14352031

申请日：2012-10-16

Applicant: Eppendorf AG

Inventor： Michael Wild ,  Christoph Jolie

IPC: G01N21/64

CPC classification number: G01N21/6486 ,  G01N21/274 ,  G01N21/64 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2201/062 ,  G01N2201/124 ,  G01N2201/127

Abstract: The invention is related to a method for the quantitative optical measurement of a characteristic property of at least one analyte in at least one laboratory sample, in particular for the fluorescence measurement of at least one biochemical or biological sample, has the method using a laboratory apparatus, which h at least one light source and at least one detector device, the apparatus utilizing at least sensitivity parameter S, which controls the capability of the laboratory apparatus to detect a signal by means of the at least one detector device, the method using source light for causing the at least one sample to emit a sample light, and the at least one detector device for detecting sample light and utilizing the at least one sensitivity parameter S to detect the corresponding at least one intensity I of the sample light, the method comprising the steps: —determining at least one reference point (S_ref; I ref); —using at least one first sensitivity parameter S_m1, which is not the same as S_ref, for measuring at least one first intensity I_m1 of sample light as-signed to a first analyte; —determining a quantity Q1, which is a measure for the slope of a line, which is determined by utilizing the at least one reference point (S_ref; 1_ref) and the at least one measurement point (S_m1; I_m1); using the quantity Q1 for calculating a first analyte value C_m1, which is dependent on Q1 and which is characteristic for a property of the first analyte, in particular for a concentration of the first analyte in the at least one sample, in particular according to the formula Q1=(I_m1−1_ref)/(S_m1−S_ref). The method, further, is related to a laboratory apparatus, which is configured to apply the method according to the invention.

Abstract translation: 本发明涉及一种用于定量光学测量至少一个实验室样品中至少一种分析物的特征性质的方法，特别是用于至少一种生化或生物样品的荧光测量的方法，具有使用实验室装置的方法 ，其至少一个光源和至少一个检测器装置，所述装置至少使用灵敏度参数S，其控制实验室装置通过至少一个检测器装置检测信号的能力，所述方法使用源 用于使所述至少一个样品发射样品光的光，以及所述至少一个检测器装置，用于检测样品光并利用所述至少一个灵敏度参数S来检测所述样品光的相应的至少一个强度I，所述方法 包括以下步骤： - 确定至少一个参考点（S_ref; I ref）; - 使用与S_ref不同的至少一个第一灵敏度参数S_m1来测量与第一分析物相符合的样本光的至少一个第一强度I_m1; - 确定通过利用所述至少一个参考点（S_ref; 1_ref）和所述至少一个测量点（S_m1; I_m1）确定的线的斜率的度量的量Q1; 使用数量Q1来计算取决于Q1的第一分析物值C_m1，其特征在于至少一个样品中的第一分析物的浓度，特别是对于至少一个样品中的第一分析物的浓度，其特征在于第一分析物的性质， 公式Q1 =（I_m1-1_ref）/（S_m1-S_ref）。 该方法还涉及实验室设备，其被配置为应用根据本发明的方法。