公开(公告)号：US20110042581A1

公开(公告)日：2011-02-24

申请号：US12851440

申请日：2010-08-05

Applicant: Masahide GUNJI

Inventor： Masahide GUNJI

IPC: G01J1/58

CPC classification number: G01J3/4406 ,  G01J3/02 ,  G01J3/0208 ,  G01J3/0229 ,  G01N21/645 ,  G01N2021/6417 ,  G01N2021/6467

Abstract: A fluorescence detector is provided to improve the non-linearity of the relationship between concentration and fluorescence intensity when the specimen concentration is high and thereby improve the dynamic range of the measurements. If it is known that the concentration of the specimen to be measured is high, a light beam restriction unit 6 whose aperture 6b in the passage direction of the excitation light Lex is short is used so that only the fluorescence being emitted from a region close to the incident end of the excitation light is condensed by the condensing lens 7 and led to the fluorescence side spectrometer 4 and detected. In this case, since the fluorescence that is emitted from a region after the passage of the excitation light through specimen solution S and its strong absorption by specimen solution S is not reflected in the measurement result, the linearity of the relationship between concentration and fluorescence intensity is improved despite the reduction in the fluorescence quantity, thus improving the quantification accuracy at high concentrations. If the specimen concentration is low, light beam restriction unit 6 with aperture 6a whose length is long in the passage direction of the excitation light Lex is used to improve sensitivity.

Abstract translation: 提供荧光检测器以提高样品浓度高时浓度和荧光强度之间的关系的非线性，从而提高测量的动态范围。 如果已知被测试样品的浓度高，则使用激发光Lex的通过方向上的孔径6b短的光束限制单元6，使得只有从接近于 激发光的入射端由聚光透镜7会聚并被引导到荧光侧光谱仪4并进行检测。 在这种情况下，由于从激发光通过试样溶液S后的区域发出的荧光及其通过试样溶液S的强烈吸收，在测定结果中没有反映出浓度与荧光强度之间的关系的线性关系 尽管荧光量降低，但是在高浓度下提高了定量精度。 如果样本浓度低，则使用具有沿激发光Lex的通过方向长度长的孔6a的光束限制单元6来提高灵敏度。

公开(公告)号：US07877135B2

公开(公告)日：2011-01-25

申请号：US11352228

申请日：2006-02-13

Applicant: Kohei Iketani ,  Mitsufumi Fukuyama

Inventor： Kohei Iketani ,  Mitsufumi Fukuyama

IPC: A61B6/00

CPC classification number: G01J3/02 ,  A61B1/00009 ,  A61B1/00039 ,  A61B1/043 ,  A61B1/045 ,  A61B1/0638 ,  A61B5/0071 ,  A61B5/0084 ,  G01J3/0232 ,  G01J3/0264 ,  G01J3/4406 ,  G01J2003/2866 ,  G01N21/6456 ,  G01N21/6486 ,  G01N2021/6423

Abstract: An image data processor comprising an image signal receiver, a histogram generator, a gain calculator, an amplifier, and a signal feeder, is provided. The image signal receiver receives an autofluorescence image signal. The autofluorescence image signal is generated by an imaging device when the imaging device captures an autofluorescence image. The histogram generator generates a histogram of luminance in the autofluorescence image based on the autofluorescence image signal. The gain calculator calculates a gain based on the histogram and a predetermined luminance value. The amplifier amplifies the autofluorescence image signal by the gain. And then the amplifier generates an amplified autofluorescence image signal. The signal feeder outputs the amplified autofluorescence image signal to a monitor. The monitor displays an amplified autofluorescence image.

Abstract translation: 提供了包括图像信号接收器，直方图发生器，增益计算器，放大器和信号馈送器的图像数据处理器。 图像信号接收器接收自发荧光图像信号。 当成像装置捕获自发荧光图像时，自动荧光图像信号由成像装置产生。 直方图发生器基于自发荧光图像信号产生自发荧光图像中的亮度直方图。 增益计算器基于直方图和预定的亮度值来计算增益。 放大器通过增益放大自发荧光图像信号。 然后放大器产生放大的自发荧光图像信号。 信号馈线将放大的自发荧光图像信号输出到监视器。 显示器显示放大的自发荧光图像。

公开(公告)号：US07872799B2

公开(公告)日：2011-01-18

申请号：US12318287

申请日：2008-12-23

Applicant: Ralf Wolleschensky

Inventor： Ralf Wolleschensky

IPC: G02B21/06

CPC classification number: G02B27/283 ,  G01J3/0224 ,  G01J3/1256 ,  G01J3/4406 ,  G01J3/447 ,  G01N21/6445 ,  G01N21/6458 ,  G02B21/002 ,  G02B21/0068

Abstract: Device for controlling light radiation, which is excited in a specimen and/or which is backscattered and/or reflected and which contains one or more wavelengths, at a plurality of light outlets, wherein a separation of the light radiation into differently polarized components is carried out; and the components of the excitation radiation and/or detection radiation are affected in their polarization by means of a preferably birefringent, preferably acousto-optic or electro-optic medium, which changes the ordinary and extraordinary refractive index.

Abstract translation: 用于控制在多个光出口处在样本中激发并且/或背散射和/或反射并且包含一个或多个波长的光辐射的装置，其中将光辐射分离成不同偏振的分量 出来 并且激发辐射和/或检测辐射的分量通过改变普通和非凡折射率的优选双折射，优选声光或电光介质在它们的极化中受到影响。

公开(公告)号：US20100330611A1

公开(公告)日：2010-12-30

申请号：US12882131

申请日：2010-09-14

Applicant: Mary-Ann Mycek ,  Malavika Chandra ,  James Scheiman ,  Robert H. Wilson ,  Diane Simeone ,  Barbara McKenna ,  Julianne Purdy ,  Jeremy Taylor ,  Oliver Lee

Inventor： Mary-Ann Mycek ,  Malavika Chandra ,  James Scheiman ,  Robert H. Wilson ,  Diane Simeone ,  Barbara McKenna ,  Julianne Purdy ,  Jeremy Taylor ,  Oliver Lee

IPC: C12Q1/02 ,  C12M1/34

CPC classification number: A61B5/415 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/0084 ,  A61B5/418 ,  A61B5/7264 ,  A61B5/7267 ,  G01J3/10 ,  G01J3/4406 ,  G01N21/474 ,  G01N21/6408 ,  G01N21/645 ,  G01N21/6486 ,  G01N33/57438 ,  G01N2021/4742 ,  G01N2021/6484 ,  G01N2201/1293

Abstract: Multimodal optical spectroscopy systems and methods produce a spectroscopic event to obtain spectroscopic response data from biological tissue and compare the response data with an empirical equation configured to correlate the measured response data and the most probable attributes of the tissue, thus facilitating classification of the tissue based on those attributes for subsequent biopsy or remedial measures as necessary.

Abstract translation: 多光谱光谱系统和方法产生光谱事件以从生物组织获得光谱响应数据，并将响应数据与配置为将测量的响应数据和组织的最可能属性相关联的经验方程进行比较，从而促进基于组织的分类 关于随后活检的这些属性或必要的补救措施。

公开(公告)号：US20100301231A1

公开(公告)日：2010-12-02

申请号：US12776657

申请日：2010-05-10

Applicant: Mitsushiro YAMAGUCHI

Inventor： Mitsushiro YAMAGUCHI

IPC: G01J1/58

CPC classification number: G01J3/4406 ,  G01N21/6408 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2021/6441 ,  G01N2201/12

Abstract: There are provided an apparatus, a method and a computer program for fluorescence correlation spectroscopy (FCS), which can reduce the number of times of fluorescence measurements of control samples as few as possible for a measurement by FCS in detecting existence ratios of the respective components contained in a sample. In the inventive apparatus, method and computer program for detecting an existence ratio of each of components with a fluorescent label contained in a solution sample by FCS, using a value of a ratio of a translational diffusion time of each of the components based upon the knowledge that a ratio of a translational diffusion time of each of the components is conservative under different measurement conditions etc.

Abstract translation: 提供了用于荧光相关光谱（FCS）的装置，方法和计算机程序，其可以尽可能少地减少控制样品的荧光测量次数，以便通过FCS测量各组分的存在比例 包含在样品中。 在本发明的装置中，用于通过FCS检测包含在溶液样品中的荧光标签的每个组分的存在比率的方法和计算机程序，使用基于知识的每个组分的平移扩散时间的比值 在不同的测量条件下，每个组分的平移扩散时间的比例是保守的

公开(公告)号：US20100253934A1

公开(公告)日：2010-10-07

申请号：US12746830

申请日：2008-12-09

Applicant: Sandro D'Ascenzi ,  Giuseppe De Conciliis ,  Claudia Magagnoli ,  Giovanni Luigi Mattioli

Inventor： Sandro D'Ascenzi ,  Giuseppe De Conciliis ,  Claudia Magagnoli ,  Giovanni Luigi Mattioli

IPC: G01J3/28 ,  G01J3/00

CPC classification number: G01N21/33 ,  A61K39/095 ,  A61K2039/6037 ,  G01J3/433 ,  G01J3/4406 ,  G01N21/6486

Abstract: The quality control of vaccines is an important step in the release of vaccines to patients. Problems when dealing with vaccines include stability, batch-to-batch consistency and contamination. The invention provides a method of analyzing the composition of a protein-containing sample by electromagnetic spectroscopy, and derivatising the obtained spectrum to obtain a derivative spectrum of the sample. The derivative spectrum may optionally be compared to a reference spectrum, to assess stability, contamination etc. relative to the reference. The derivative analysis technique allows the separation and discrimination between the spectral contributions of different components, thereby allowing differences in the spectra to be assigned to particular groups of the protein, or particular contaminants etc.

Abstract translation: 疫苗的质量控制是向患者释放疫苗的重要一步。 处理疫苗时的问题包括稳定性，批次间的一致性和污染。 本发明提供了一种通过电磁光谱分析含蛋白质样品的组成并衍生得到的光谱以获得样品的衍生光谱的方法。 衍生光谱可以任选地与参考光谱进行比较，以评估相对于参考物的稳定性，污染等。 衍生分析技术允许分离和区分不同成分的光谱贡献，从而允许将光谱差异分配给蛋白质的特定组或特定的污染物等。

公开(公告)号：US20100145416A1

公开(公告)日：2010-06-10

申请号：US12473745

申请日：2009-05-28

Applicant: Uk KANG ,  Garri. V Papayan

Inventor： Uk KANG ,  Garri. V Papayan

IPC: A61N5/067 ,  A61N5/06

CPC classification number: G01N21/6486 ,  A61B5/0059 ,  A61N5/0601 ,  A61N5/062 ,  G01J3/02 ,  G01J3/021 ,  G01J3/0248 ,  G01J3/10 ,  G01J3/12 ,  G01J3/36 ,  G01J3/4406 ,  G01N21/255 ,  G01N21/29 ,  G01N21/31 ,  G01N21/4738 ,  G01N21/6456 ,  G01N2201/0618

Abstract: The present invention provides an apparatus for photodynamic therapy and fluorescence detection, in which a combined light source is provided to illuminate an object body and a multispectral fluorescence-reflectance image is provided to reproduce various and complex spectral images for an object tissue, thus performing effective photodynamic therapy for various diseases both outside and inside of the body.For this purpose, the present invention provides an apparatus for photodynamic therapy and photodetection, which provides illumination with light of various wavelengths and multispectral images, the apparatus including: an optical imaging system producing an image of an object tissue and transmitting the image to a naked eye or an imaging device; a combined light source including a plurality of coherent and non-coherent light sources and a light guide guiding incident light emitted from the light sources; a multispectral imaging system including at least one image sensor; and a computer system outputting an image of the object tissue to the outside. Thus, the apparatus for photodynamic therapy and photodetection of the present invention can effectively perform the photodynamic therapy and photodetection by means of the combined light source capable of irradiating light having various spectral components to an object tissue and the multispectral imaging system capable of obtaining images from several spectral portions for these various spectral ranges at the same time, thus improving the accuracy of diagnosis and efficiency of the photodynamic therapy.

Abstract translation: 本发明提供了一种用于光动力学治疗和荧光检测的装置，其中提供组合光源以照射物体，并且提供多光谱荧光反射图像以再现对象组织的各种和复杂的光谱图像，从而执行有效 身体各种疾病的光动力治疗。 为此，本发明提供了一种用于光动力学治疗和光电检测的装置，其提供具有各种波长和多光谱图像的光的照明，所述装置包括：光学成像系统，其产生对象组织的图像并将图像发送到裸体 眼睛或成像装置; 包括多个相干和非相干光源的组合光源和引导从光源发射的入射光的光导; 包括至少一个图像传感器的多光谱成像系统; 以及将对象组织的图像输出到外部的计算机系统。 因此，本发明的光动力学治疗和光电检测装置可以通过能够将具有各种光谱成分的光照射到对象组织上的组合光源和能够获得图像的多光谱成像系统而有效地进行光动力学治疗和光电检测 同时对于这些不同光谱范围的几个光谱部分，从而提高光动力疗法的诊断准确性和效率。

公开(公告)号：US20100118301A1

公开(公告)日：2010-05-13

申请号：US12270800

申请日：2008-11-13

Applicant: Jan Vondras ,  Sean Rick

Inventor： Jan Vondras ,  Sean Rick

IPC: G01J3/30 ,  G01C25/00

CPC classification number: G01J3/10 ,  G01J3/4406 ,  G01N21/274 ,  G01N21/645 ,  G01N21/76 ,  G01N33/0042 ,  G01N2201/06113

Abstract: A system and associated method are disclosed for analyzing a sample or sample component including species capable of producing fluorescent light when excited by a light source, where the light source comprises an excimer light source having a high voltage power supply with voltage and current regulation circuitry.

Abstract translation: 公开了一种用于分析样品或样品组分的系统和相关方法，所述样品或样品组分包括当由光源激发时能够产生荧光的物质，其中所述光源包括具有电压和电流调节电路的具有高电压电源的准分子光源。

公开(公告)号：US20100111369A1

公开(公告)日：2010-05-06

申请号：US12567931

申请日：2009-09-28

Applicant: Robert Lussier

Inventor： Robert Lussier

IPC: G06K9/62 ,  G01J1/58

CPC classification number: G01J3/4406 ,  G01J3/02 ,  G01J3/0272 ,  G01J3/10 ,  G01J3/2823 ,  G01J3/42 ,  G01N21/31 ,  G01N21/3563 ,  G01N21/359 ,  G01N21/6456 ,  G01N21/6486 ,  G01N2021/635 ,  G01N2021/8466

Abstract: A portable fluorescence and transmittance imaging spectroscopy system for use in diagnosing plant health. The system has a primary LED light source array with spectral wavelengths in the 400-600 nm range, a focus cone that collects the LED light source output and focuses it, a controller that controls the primary LED array to turn it on and off, or certain of the spectral wavelengths on and off such that the primary LED array controllably emits light of a desired wavelength in the range, the light irradiating the plant through the focus cone, a digital imaging device that both spatially and temporally captures a fluorescence image comprising chlorophyll fluorescence emitted by the plant due to the emitted light from the LED array, a leaf holder located proximate to the output of the focus cone to maintain a consistent position and distance between the digital imaging device, the LED light source and the leaf and providing for fixed position and non-destructive leaf imaging and testing, a secondary light source for providing broad-band transmissive light through the leaf, a lens for focusing onto the imaging device the light emitted from the secondary light source, and one or more memory devices that store the fluorescence image and the transmitted light data received by the digital imaging device and store a library of plant fluorescence-intensity data indicative of both healthy plants and stressed or diseased plants, and plant light transmittance data indicative of certain plant conditions.

Abstract translation: 便携式荧光和透射成像光谱系统，用于诊断植物健康。 该系统具有400-600nm范围内的光谱波长的主要LED光源阵列，聚焦锥体，用于收集LED光源输出并对其进行聚焦，该控制器控制主要LED阵列的打开和关闭，或 某些光谱波长开启和关闭，使得主LED阵列可控制地发射在该范围内的光，通过聚焦锥照射植物的光;数字成像装置，其在空间和时间上捕获包括叶绿素的荧光图像 由于来自LED阵列的发射光，植物发出的荧光，位于焦点锥体的输出附近的叶片保持器，以保持数字成像装置，LED光源和叶片之间的一致的位置和距离，并提供 固定位置和非破坏性叶成像和测试，用于通过叶片提供宽带透射光的二次光源，用于聚焦的透镜 将来自二次光源的光发射到成像装置上，以及存储由数字成像装置接收的荧光图像和透射光数据的一个或多个存储装置，并存储指示健康的植物荧光强度数据库 植物和受胁迫或患病植物，以及指示某些植物条件的植物光透射率数据。

公开(公告)号：US07709821B2

公开(公告)日：2010-05-04

申请号：US11380434

申请日：2006-04-27

Applicant: Martin K. Casstevens ,  Ryszard Burzynski ,  John Weibel ,  Alexander Kachynski

Inventor： Martin K. Casstevens ,  Ryszard Burzynski ,  John Weibel ,  Alexander Kachynski

IPC: G01N21/49 ,  G01J1/58

CPC classification number: G01J3/02 ,  G01J3/0218 ,  G01J3/4406 ,  G01N15/1459 ,  G01N15/1463 ,  G01N21/645

Abstract: A flow cytometer has a flow cell through which a sample flows and at least one laser emitting an excitation beam for illuminating a corresponding interrogation region in the flow cell. Scattered and fluorescence light from each interrogation region is collected by one or more input fibers for that region, and the input fiber(s) are fed to a dispersion module for that interrogation region that disperses the incoming light into different spectral regions. The dispersed light is conveyed, such as by a plurality of output fibers, to one or more photosensitive detectors. Thus, time multiplexed light signals may be delivered to a detector whereby several unique light signals can be measured by a single detector.

Abstract translation: 流式细胞仪具有流动池，样品流过该流动池，并且至少一个激光发射激发光束，用于照射流动池中的对应询问区域。 来自每个询问区域的散射光和荧光由该区域的一个或多个输入光纤收集，并且输入光纤被馈送到用于将入射光分散到不同光谱区域的询问区域的色散模块。 分散的光通过多个输出光纤传送到一个或多个光敏检测器。 因此，时间复用光信号可以传送到检测器，由此可以通过单个检测器测量几个独特的光信号。