HIGH RESOLUTION IMAGING MASS SPECTROMETRY
    91.
    发明申请

    公开(公告)号:US20170084435A1

    公开(公告)日:2017-03-23

    申请号:US15268097

    申请日:2016-09-16

    申请人: BAYLOR UNIVERSITY

    发明人: Touradj SOLOUKI

    IPC分类号: H01J49/00 H01J49/10

    摘要: The present disclosure provides a system and method for mass spectrometry imaging in a multi-stage ionization applying different technologies by decoupling the desorption and ionization events. At a first stage, a primary beam, such as an ion beam, desorbs one or more molecules of a targeted sample, and at a second stage the desorbed molecules are ionized. The system and method can act independent of a matrix application to the target sample for a direct analysis and has the spatial resolution needed to operate in nano-meters resolution for a cell-by-cell analysis, if desired. The first stage desorption applies a first technique that allows neutral molecules of the target sample to become desorbed from the surface without requiring the molecules to be ionized during the desorption. The second stage ionizes the neutral molecules after the desorption in the first stage, when the defined target molecules have been volatilized.

    REMOTE LASER ABLATION ELECTROSPRAY IONIZATION MASS SPECTROMETRY
    92.
    发明申请
    REMOTE LASER ABLATION ELECTROSPRAY IONIZATION MASS SPECTROMETRY 审中-公开
    远红外激光电泳离子质谱法

    公开(公告)号:US20160203966A1

    公开(公告)日:2016-07-14

    申请号:US14912506

    申请日:2014-08-26

    IPC分类号: H01J49/04 H01J49/16

    CPC分类号: H01J49/0463 H01J49/165

    摘要: In various embodiments, a device may generally comprise a remote ablation chamber comprising an inlet and an outlet, a laser to deposit energy into a sample in the chamber to ablate the sample and generate ablation products in the chamber, a transport device in fluid communication with the outlet, an ionization source to ionize the ablation products to produce ions, and a mass spectrometer having an ion transfer inlet to capture the ions. The ablation products or the ions may be transported in a fluid stream from the ablation chamber through the transport device. The distance from the outlet of the ablation chamber to the ion transfer inlet may be from 1 cm to 10 m. Methods of making and using the same are also described.

    摘要翻译: 在各种实施例中,装置通常可包括远端消融腔室,其包括入口和出口,激光器,以将能量沉积到腔室中的样品中以烧蚀样品并在腔室中产生消融产物,输送装置与 出口,用于电离消融产物以产生离子的电离源,以及具有离子转移入口捕获离子的质谱仪。 消融产物或离子可以以流体流从消融室经由输送装置输送。 从消融室的出口到离子转移入口的距离可以为1cm至10μm。 还描述了制备和使用它们的方法。

    MALDI Imaging and Ion Source
    93.
    发明申请
    MALDI Imaging and Ion Source 有权
    MALDI成像和离子源

    公开(公告)号:US20150034814A1

    公开(公告)日:2015-02-05

    申请号:US14130453

    申请日:2012-07-06

    摘要: An ion source for a mass spectrometer is disclosed comprising a lens and mirror arrangement which focuses a laser beam onto the upper surface of a target substrate. The lens has an effective focal length ≦300 mm. The laser beam is directed onto the target substrate at an angle θ with respect to the perpendicular to the target substrate, wherein θ≦3°. One or more ion guides receive ions released from the target substrate and onwardly transmit the ions along an ion path which substantially bypasses the lens and mirror.

    摘要翻译: 公开了一种用于质谱仪的离子源,其包括将激光束聚焦到目标衬底的上表面上的透镜和反射镜装置。 镜头具有有效的焦距≦̸ 300 mm。 激光束以一定角度被引导到靶基底上; 相对于与靶基板的垂直度,其中& nlE; 3°。 一个或多个离子导向器接收从目标衬底释放的离子,并沿基本上绕过透镜和反射镜的离子路径向上透射离子。

    LASER SAMPLING METHODS FOR REDUCING THERMAL EFFECTS
    95.
    发明申请
    LASER SAMPLING METHODS FOR REDUCING THERMAL EFFECTS 审中-公开
    用于减少热效应的激光采样方法

    公开(公告)号:US20140268134A1

    公开(公告)日:2014-09-18

    申请号:US14209843

    申请日:2014-03-13

    IPC分类号: G01J3/443

    摘要: A method for reducing thermal effects in laser ablation optical emission spectrometry includes creating discrete ablation spots along an analysis line on a target surface. At least one of the following is also carried out. First, the ablation spots are positioned so that a pair of successive ablation spots are spaced apart from one another along the analysis line and are separated from one another by another ablation spot. Second, when the analysis line comprises generally parallel, adjacent analysis line segments, the ablation spots are positioned so that (A) a pair of successive ablation spots are on different analysis line segments, and (B) the successive ablation spots are positioned to be at different longitudinal positions along the analysis line segments when the different analysis line segments are adjacent to one another. As a result, a linear scan of isolated ablation spots can be generated.

    摘要翻译: 用于减少激光烧蚀光学发射光谱法中热效应的方法包括沿目标表面上的分析线创建离散的消融点。 还执行以下中的至少一个。 首先,消融点被定位成使得一对连续的消融点沿着分析线彼此间隔开并且通过另一个消融点彼此分离。 第二,当分析线包括大致平行的相邻分析线段时,消融点被定位成使得(A)一对连续的消融点位于不同的分析线段上,并且(B)连续的消融点被定位为 在不同分析线段彼此相邻时沿着分析线段的不同纵向位置。 结果,可以产生孤立的消融点的线性扫描。

    Plume collimation for laser ablation electrospray ionization mass spectrometry
    96.
    发明授权
    Plume collimation for laser ablation electrospray ionization mass spectrometry 有权
    激光消融电喷雾电离质谱法的羽流准直

    公开(公告)号:US08829426B2

    公开(公告)日:2014-09-09

    申请号:US13549988

    申请日:2012-07-16

    IPC分类号: H01J49/16 H01J49/10

    摘要: In various embodiments, a device may generally comprise a capillary having a first end and a second end; a laser to emit energy at a sample in the capillary to ablate the sample and generate an ablation plume in the capillary; an electrospray apparatus to generate an electrospray plume to intercept the ablation plume to produce ions; and a mass spectrometer having an ion transfer inlet to capture the ions. The ablation plume may comprise a collimated ablation plume. The device may comprise a flow cytometer. Methods of making and using the same are also described.

    摘要翻译: 在各种实施例中,装置通常可以包括具有第一端和第二端的毛细管; 在毛细管中的样品处发射能量的激光以烧蚀样品并在毛细管中产生消融羽流; 电喷射装置,用于产生电喷雾羽流以拦截消融羽流以产生离子; 和具有离子转移入口以捕获离子的质谱仪。 消融羽流可以包括准直的消融羽流。 该装置可以包括流式细胞仪。 还描述了制备和使用它们的方法。

    Spinning cell device for fast standardization in laser ablation inductively coupled plasma spectrometry
    99.
    发明授权
    Spinning cell device for fast standardization in laser ablation inductively coupled plasma spectrometry 有权
    旋转电池器件,用于激光烧蚀电感耦合等离子体光谱法中的快速标准化

    公开(公告)号:US08742334B2

    公开(公告)日:2014-06-03

    申请号:US13867012

    申请日:2013-04-19

    摘要: A spinning cell device is described for fast and convenient standardization and analysis of constituents and isotopes in solid samples by laser ablation inductively coupled plasma (LA-ICP) spectrometry. The method and apparatus for performing the method require the sample under test and a standard to be spun during ablation allowing the quasi-simultaneous ablation of both materials. The aerosols resulting from the ablation of sample and standard are mixed in the ablation cell allowing quantification of the ablated metals by the method of standard addition or isotope dilution. The relative proportion of standard verses sample ablated can be changed by altering the trajectory of the laser beam. The ablated aerosol is swept into an inductively coupled plasma by a carrier gas and analyzed by mass spectrometry.

    摘要翻译: 描述了通过激光烧蚀电感耦合等离子体(LA-ICP)光谱法快速便捷地对固体样品中成分和同位素进行标准化和分析的旋转电池器件。 用于执行该方法的方法和装置需要待测样品和在消融期间旋转的标准,以允许两种材料的准同时消融。 将消融样品和标准物产生的气溶胶混合在消融细胞中,允许通过标准添加或同位素稀释的方法定量烧伤的金属。 可以通过改变激光束的轨迹来改变烧蚀样品的相对比例。 通过载气将烧蚀的气溶胶扫入电感耦合等离子体,并通过质谱法进行分析。