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    METHODS FOR PRODUCING MODIFIED GLYCOPROTEINS
    94.
    发明申请
    METHODS FOR PRODUCING MODIFIED GLYCOPROTEINS 审中-公开
    生产改性糖蛋白的方法

    公开(公告)号：US20130295604A1

    公开(公告)日：2013-11-07

    申请号：US13934551

    申请日：2013-07-03

    申请人： Tillman U. Gerngross

    发明人： Tillman U. Gerngross

    IPC分类号： C12P21/00

    CPC分类号： C12P21/005 ,  C07K2319/04 ,  C07K2319/05 ,  C12N1/14 ,  C12N9/1048 ,  C12N9/2488 ,  C12N15/79 ,  C12N15/80 ,  C12N15/81 ,  C12Y302/01 ,  C12Y302/01113

    摘要： Cell lines having genetically modified glycosylation pathways that allow them to carry out a sequence of enzymatic reactions, which mimic the processing of glycoproteins in humans, have been developed. Recombinant proteins expressed in these engineered hosts yield glycoproteins more similar, if not substantially identical, to their human counterparts. The lower eukaryotes, which ordinarily produce high-mannose containing N-glycans, including unicellular and multicellular fungi are modified to produce N-glycans such as Man5GlcNAc2 or other structures along human glycosylation pathways. This is achieved using a combination of engineering and/or selection of strains which: do not express certain enzymes which create the undesirable complex structures characteristic of the fungal glycoproteins, which express exogenous enzymes selected either to have optimal activity under the conditions present in the fungi where activity is desired, or which are targeted to an organelle where optimal activity is achieved, and combinations thereof wherein the genetically engineered eukaryote expresses multiple exogenous enzymes required to produce “human-like” glycoproteins.

    摘要翻译： 已经开发了具有遗传修饰的糖基化途径的细胞系，其允许它们进行模拟人类中糖蛋白的加工的酶反应序列。 在这些工程化宿主中表达的重组蛋白可以产生与其对应物更相似的糖蛋白（如果基本上不相同）。 通常产生含有高甘露糖的N-聚糖（包括单细胞和多细胞真菌）的低等真核生物被修饰以产生N-聚糖如Man5GlcNAc2或沿人糖基化途径的其它结构。 这是使用以下工程和/或选择的组合实现的：不表达产生真菌糖蛋白特征的不合需要的复合结构的某些酶，其表达选择在真菌中存在的条件下具有最佳活性的外源酶 其中需要活性或靶向实现最佳活性的细胞器，以及其组合，其中遗传工程真核生物表达产生“人样”糖蛋白所需的多种外源酶。

    Combinatorial DNA Library for Producing Modified N-Glycans In Lower Eukaryotes
    96.
    发明申请
    Combinatorial DNA Library for Producing Modified N-Glycans In Lower Eukaryotes 审中-公开
    用于在低等真核生物中生产修饰的N-糖的组合DNA文库

    公开(公告)号：US20130217067A1

    公开(公告)日：2013-08-22

    申请号：US13408432

    申请日：2012-02-29

    申请人： TILLMAN U. GERNGROSS ,  Stefan Wildt ,  Byung-kwon Choi ,  Juergen Hermann Nett ,  Piotr Bobrowicz ,  Stephen R. Hamilton ,  Robert C. Davidson

    发明人： TILLMAN U. GERNGROSS ,  Stefan Wildt ,  Byung-kwon Choi ,  Juergen Hermann Nett ,  Piotr Bobrowicz ,  Stephen R. Hamilton ,  Robert C. Davidson

    IPC分类号： C07K14/47

    CPC分类号： C12P21/005 ,  C07K14/47 ,  C12N9/1051 ,  C12N15/1082 ,  C12N15/79

    摘要： The present invention relates to eukaryotic host cells having modified oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar transporters and mannosidases to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The invention provides nucleic acid molecules and combinatorial libraries which can be used to successfully target and express mammalian enzymatic activities such as those involved in glycosylation to intracellular compartments in a eukaryotic host cell. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified oligosaccharides are created or selected. N-glycans made in the engineered host cells have a Man5GlcNAc2 core structure which may then be modified further by heterologous expression of one or more enzymes, e.g., glycosyltransferases, sugar transporters and mannosidases, to yield human-like glycoproteins.

    摘要翻译： 本发明涉及具有修饰的寡糖的真核宿主细胞，其可以通过异源表达一组糖基转移酶，糖转运体和甘露糖苷酶进一步修饰，以成为用于产生哺乳动物例如人治疗性糖蛋白的宿主菌株。 本发明提供核酸分子和组合文库，其可用于成功靶向和表达哺乳动物酶活性，例如参与糖基化的真核宿主细胞中的细胞内区室的那些。 该方法提供了可用于表达和靶向参与糖基化的任何所需基因的工程化宿主细胞。 建立或选择具有修饰寡糖的宿主细胞。 在工程化宿主细胞中制备的N-聚糖具有Man5GlcNAc2核心结构，然后可以通过异源表达一种或多种酶，例如糖基转移酶，糖转运蛋白和甘露糖苷酶来进一步修饰，以产生人样糖蛋白。

    MAGNETOTACTIC ALGAE AND METHODS OF USE
    98.
    发明申请
    MAGNETOTACTIC ALGAE AND METHODS OF USE 审中-公开
    磁性材料及其使用方法

    公开(公告)号：US20130210064A1

    公开(公告)日：2013-08-15

    申请号：US13494533

    申请日：2012-06-12

    申请人： Pulak Nath ,  Scott N. Twary

    发明人： Pulak Nath ,  Scott N. Twary

    IPC分类号： C12P7/64 ,  C12R1/89 ,  C12P3/00 ,  C12N15/79

    CPC分类号： C12P7/6409 ,  C07K14/195 ,  C12N1/12 ,  C12N5/04 ,  C12N15/79 ,  C12N15/8242 ,  C12P1/00 ,  C12P3/00 ,  C12P7/6463 ,  C12R1/89

    摘要： Disclosed herein are magnetotactic algae, such as algae cells that include magnetic nanoparticles. In some examples the magnetotactic algae express a nucleic acid molecule encoding a bacterial MagA ferrous transporter, a nucleic acid molecule encoding a bacterial Mms6 magnetite binding protein, or both. Also disclosed herein are methods for producing magnetotactic algae and methods of producing biofuel or magnetic nanoparticles utilizing magnetotactic algae. Further disclosed herein are methods of enriching a population of magnetotactic algae cells (for example, increasing the number of magnetotactic algae cells in a population of algae cells). In further embodiments, disclosed herein are methods of selecting a transformed algae cell.

    摘要翻译： 本文公开的是磁性藻类，例如包括磁性纳米颗粒的藻类细胞。 在一些实例中，磁刺激藻类表达编码细菌MagA亚铁转运蛋白的核酸分子，编码细菌Mms6磁铁矿结合蛋白的核酸分子或两者。 本文还公开了用于产生磁脑藻的方法和利用磁脑藻类生产生物燃料或磁性纳米颗粒的方法。 这里进一步公开的是富集大面积藻类细胞群的方法（例如，增加藻类细胞群体中的趋磁藻细胞的数量）。 在另外的实施方案中，本文公开了选择转化的藻类细胞的方法。