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公开(公告)号：US20130143306A1
公开(公告)日：2013-06-06
申请号：US13765211
申请日：2013-02-12
申请人： David Arthur Berry , Noubar Boghos Afeyan , Frank Anthony Skraly , Christian Perry Ridley , Dan Eric Robertson , Regina Wilpiszeski , Martha Sholl
发明人： David Arthur Berry , Noubar Boghos Afeyan , Frank Anthony Skraly , Christian Perry Ridley , Dan Eric Robertson , Regina Wilpiszeski , Martha Sholl
IPC分类号： C12N15/79
CPC分类号： C10L1/026 , C10L2200/0476 , C10L2270/026 , C12N15/79 , C12P7/649 , C12Y602/01003 , Y02E50/13 , Y02E50/17
摘要： The present disclosure identifies methods and compositions for modifying photoautotrophic organisms, such that the organisms efficiently convert carbon dioxide and light into compounds such as esters and fatty acids. In certain embodiments, the compounds produced are secreted into the medium used to culture the organisms.
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公开(公告)号：US08741624B2
公开(公告)日：2014-06-03
申请号：US12539079
申请日：2009-08-11
申请人： Frank A. Skraly , Martha Sholl
发明人： Frank A. Skraly , Martha Sholl
摘要： Recombinant processes are provided whereby additional genes are introduced into E. coli which have been genetically engineered to produce PHA so that the improved strains produce PHA homopolymers and copolymers directly from diols. In preferred embodiments, PHAs containing 4-hydroxybutyrate monomers are produced directly from 1,4-butanediol; PHAs containing 5-hydroxyvalerate are produced from 1,5-pentanediol; PHAs containing 6-hydroxyhexanoate (6HH) are produced from 1,6-hexanediol; PHAs containing 3-hydroxypropionate are produced from 1,3-propanediol; PHAs containing 2-hydroxypropionate (lactate) are produced from 1,2-propanediol (propylene glycol); PHAs containing 2-hydroxyethanoate (glycolate) are produced from 1,2-ethanediol (ethylene glycol). Genes encoding these same enzyme activities can be introduced or their expression amplified in wild type PHA producers to improve the production of PHA homopolymers and copolymers directly from diol and other alcohol feedstocks. The PHA polymers are readily recovered and industrially useful as polymers or as starting materials for a range of chemical intermediates.
摘要翻译： 提供了重组方法，其中将另外的基因导入大肠杆菌，其已被遗传工程化以产生PHA，使得改良的菌株直接从二醇产生PHA均聚物和共聚物。 在优选的实施方案中，含有4-羟基丁酸酯单体的PHA直接由1,4-丁二醇制备; 含有5-羟基戊酸酯的PHA由1,5-戊二醇制备; 含有6-羟基己酸（6HH）的PHA由1,6-己二醇制成; 含有3-羟基丙酸的PHAs由1,3-丙二醇生产; 含有2-羟基丙酸酯（乳酸酯）的PHA由1,2-丙二醇（丙二醇）制成; 含有2-羟基乙醇酸（乙醇酸）的PHA由1,2-乙二醇（乙二醇）制备。 可以在野生型PHA生产者中引入编码这些相同酶活性的基因或其表达，以直接从二醇和其它醇原料改进PHA均聚物和共聚物的生产。 PHA聚合物易于回收并且在工业上可用作聚合物或作为一系列化学中间体的起始材料。
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3.发明授权Methods and compositions for the recombinant biosynthesis of fatty acids and esters 有权用于脂肪酸和酯的重组生物合成的方法和组合物公开(公告)号：US08048654B2
公开(公告)日：2011-11-01
申请号：US12876056
申请日：2010-09-03
申请人： David Arthur Berry , Noubar Boghos Afeyan , Frank Anthony Skraly , Christian Perry Ridley , Dan Eric Robertson , Regina Wilpiszeski , Martha Sholl
发明人： David Arthur Berry , Noubar Boghos Afeyan , Frank Anthony Skraly , Christian Perry Ridley , Dan Eric Robertson , Regina Wilpiszeski , Martha Sholl
IPC分类号： C12P7/64
CPC分类号： C10L1/026 , C10L2200/0476 , C10L2270/026 , C12N15/79 , C12P7/649 , C12Y602/01003 , Y02E50/13 , Y02E50/17
摘要： The present disclosure identifies methods and compositions for modifying photoautotrophic organisms, such that the organisms efficiently convert carbon dioxide and light into compounds such as esters and fatty acids. In certain embodiments, the compounds produced are secreted into the medium used to culture the organisms.
摘要翻译： 本公开内容确定了修饰光自养生物的方法和组合物，使得生物有效地将二氧化碳和光转化为化合物如酯和脂肪酸。 在某些实施方案中，所生产的化合物分泌到用于培养生物体的培养基中。
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4.发明申请Engineered CO2 Fixing Microorganisms Producing Carbon-Based Products of Interest 有权工程化的CO2固定微生物生产碳基产品公开(公告)号：US20110008861A1
公开(公告)日：2011-01-13
申请号：US12867738
申请日：2009-03-03
申请人： David A. Berry , Dan E. Robertson , Frank A. Skraly , Brian D. Green , Christian P. Ridley , Sriram Kosuri , Nikos B. Reppas , Martha Sholl , Noubar B. Afeyan
发明人： David A. Berry , Dan E. Robertson , Frank A. Skraly , Brian D. Green , Christian P. Ridley , Sriram Kosuri , Nikos B. Reppas , Martha Sholl , Noubar B. Afeyan
CPC分类号： C12P7/065 , C12N1/20 , C12N9/00 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/0083 , C12N9/1007 , C12N9/1029 , C12N9/88 , C12N15/74 , C12P1/04 , C12P5/00 , C12P5/02 , C12P5/026 , C12P7/00 , C12P7/6409 , C12P7/6427 , C12P7/6436 , C12P7/6463 , C12P7/6472 , C12P7/649 , C12P13/04 , C12R1/01 , C12Y103/03006 , Y02E50/13 , Y02E50/17 , Y02E50/18 , Y02E50/343
摘要： The present disclosure identifies pathways and mechanisms to confer production of carbon-based products of interest such as ethanol, ethylene, chemicals, polymers, n-alkanes, isoprenoids, pharmaceutical products or intermediates thereof in photoautotrophic organisms such that these organisms efficiently convert carbon dioxide and light into carbon-based products of interest, and in particular the use of such organisms for the commercial production of ethanol, ethylene, chemicals, polymers, alkanes, isoprenoids, pharmaceutical products or intermediates thereof.
摘要翻译： 本发明公开了确定感兴趣的碳基产物如乙醇，乙烯，化学品，聚合物，正烷烃，类异戊二烯，药物产品或其中间体在光自养生物体中生产的途径和机制，使得这些生物有效地转化二氧化碳和 特别是利用这些生物来商业生产乙醇，乙烯，化学品，聚合物，烷烃，类异戊二烯，药物产品或其中间体。
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公开(公告)号：US20100021919A1
公开(公告)日：2010-01-28
申请号：US12539079
申请日：2009-08-11
申请人： Frank A. Skraly , Martha Sholl
发明人： Frank A. Skraly , Martha Sholl
摘要： Recombinant processes are provided whereby additional genes are introduced into E. coli which have been genetically engineered to produce PHA so that the improved strains produce PHA homopolymers and copolymers directly from diols. In preferred embodiments, PHAs containing 4-hydroxybutyrate monomers are produced directly from 1,4-butanediol; PHAs containing 5-hydroxyvalerate are produced from 1,5-pentanediol; PHAs containing 6-hydroxyhexanoate (6HH) are produced from 1,6-hexanediol; PHAs containing 3-hydroxypropionate are produced from 1,3-propanediol; PHAs containing 2-hydroxypropionate (lactate) are produced from 1,2-propanediol (propylene glycol); PHAs containing 2-hydroxyethanoate (glycolate) are produced from 1,2-ethanediol (ethylene glycol). Genes encoding these same enzyme activities can be introduced or their expression amplified in wild type PHA producers to improve the production of PHA homopolymers and copolymers directly from diol and other alcohol feedstocks. The PHA polymers are readily recovered and industrially useful as polymers or as starting materials for a range of chemical intermediates.
摘要翻译： 提供了重组方法，其中将另外的基因导入大肠杆菌，其已被遗传工程化以产生PHA，使得改良的菌株直接从二醇产生PHA均聚物和共聚物。 在优选的实施方案中，含有4-羟基丁酸酯单体的PHA直接由1,4-丁二醇制备; 含有5-羟基戊酸酯的PHA由1,5-戊二醇制备; 含有6-羟基己酸（6HH）的PHA由1,6-己二醇制成; 含有3-羟基丙酸的PHAs由1,3-丙二醇生产; 含有2-羟基丙酸酯（乳酸酯）的PHA由1,2-丙二醇（丙二醇）制成; 含有2-羟基乙醇酸（乙醇酸）的PHA由1,2-乙二醇（乙二醇）制备。 可以在野生型PHA生产者中引入编码这些相同酶活性的基因或其表达，以直接从二醇和其它醇原料改进PHA均聚物和共聚物的生产。 PHA聚合物易于回收并且在工业上可用作聚合物或作为一系列化学中间体的起始材料。
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6.发明授权Engineered CO2 fixing microorganisms producing carbon-based products of interest 有权工程化的二氧化碳固定微生物生产碳基产品公开(公告)号：US09150888B2
公开(公告)日：2015-10-06
申请号：US13349545
申请日：2012-01-12
申请人： David Arthur Berry , Dan Eric Robertson , Frank Anthony Skraly , Brian D. Green , Christian Perry Ridley , Sriram Kosuri , Nikos Basil Reppas , Martha Sholl , Noubar Boghos Afeyan
发明人： David Arthur Berry , Dan Eric Robertson , Frank Anthony Skraly , Brian D. Green , Christian Perry Ridley , Sriram Kosuri , Nikos Basil Reppas , Martha Sholl , Noubar Boghos Afeyan
IPC分类号： C12N1/13 , C12P7/06 , C12N1/20 , C12N9/00 , C12N9/04 , C12N9/02 , C12N9/10 , C12N9/88 , C12P1/04 , C12P5/00 , C12P7/00 , C12P7/64 , C12P13/04 , C12R1/01 , C12P5/02 , C12N15/74
CPC分类号： C12P7/065 , C12N1/20 , C12N9/00 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/0083 , C12N9/1007 , C12N9/1029 , C12N9/88 , C12N15/74 , C12P1/04 , C12P5/00 , C12P5/02 , C12P5/026 , C12P7/00 , C12P7/6409 , C12P7/6427 , C12P7/6436 , C12P7/6463 , C12P7/6472 , C12P7/649 , C12P13/04 , C12R1/01 , C12Y103/03006 , Y02E50/13 , Y02E50/17 , Y02E50/18 , Y02E50/343
摘要： The present disclosure identifies pathways and mechanisms to confer production of carbon-based products of interest such as ethanol, ethylene, chemicals, polymers, n-alkanes, isoprenoids, pharmaceutical products or intermediates thereof in photoautotrophic organisms such that these organisms efficiently convert carbon dioxide and light into carbon-based products of interest, and in particular the use of such organisms for the commercial production of ethanol, ethylene, chemicals, polymers, n-alkanes, isoprenoids, pharmaceutical products or intermediates thereof.
摘要翻译： 本发明公开了确定感兴趣的碳基产物如乙醇，乙烯，化学品，聚合物，正烷烃，类异戊二烯，药物产品或其中间体在光自养生物体中生产的途径和机制，使得这些生物有效地转化二氧化碳和 特别是利用这些有机物用于商业生产乙醇，乙烯，化学品，聚合物，正烷烃，类异戊二烯，药物产品或其中间体。
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7.发明授权Methods and compositions for the recombinant biosynthesis of fatty acids and esters 有权用于脂肪酸和酯的重组生物合成的方法和组合物公开(公告)号：US08906665B2
公开(公告)日：2014-12-09
申请号：US13765211
申请日：2013-02-12
申请人： David Arthur Berry , Noubar Boghos Afeyan , Frank Anthony Skraly , Christian Perry Ridley , Dan Eric Robertson , Regina Wilpiszeski , Martha Sholl
发明人： David Arthur Berry , Noubar Boghos Afeyan , Frank Anthony Skraly , Christian Perry Ridley , Dan Eric Robertson , Regina Wilpiszeski , Martha Sholl
CPC分类号： C10L1/026 , C10L2200/0476 , C10L2270/026 , C12N15/79 , C12P7/649 , C12Y602/01003 , Y02E50/13 , Y02E50/17
摘要： The present disclosure identifies methods and compositions for modifying photoautotrophic organisms, such that the organisms efficiently convert carbon dioxide and light into compounds such as esters and fatty acids. In certain embodiments, the compounds produced are secreted into the medium used to culture the organisms.
摘要翻译： 本公开内容确定了修饰光自养生物的方法和组合物，使得生物有效地将二氧化碳和光转化为化合物如酯和脂肪酸。 在某些实施方案中，所生产的化合物分泌到用于培养生物体的培养基中。
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8.发明申请ENGINEERED CO2 FIXING MICROORGANISMS PRODUCING CARBON-BASED PRODUCTS OF INTEREST 有权工程化二氧化碳固定微生物生产基于碳的产品公开(公告)号：US20120122193A1
公开(公告)日：2012-05-17
申请号：US13349545
申请日：2012-01-12
申请人： David A. Berry , Dan E. Robertson , Frank A. Skraly , Brian D. Green , Christian P. Ridley , Sriram Kosuri , Nikos B. Reppas , Martha Sholl , Noubar B. Afeyan
发明人： David A. Berry , Dan E. Robertson , Frank A. Skraly , Brian D. Green , Christian P. Ridley , Sriram Kosuri , Nikos B. Reppas , Martha Sholl , Noubar B. Afeyan
IPC分类号： C12N1/13
CPC分类号： C12P7/065 , C12N1/20 , C12N9/00 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/0083 , C12N9/1007 , C12N9/1029 , C12N9/88 , C12N15/74 , C12P1/04 , C12P5/00 , C12P5/02 , C12P5/026 , C12P7/00 , C12P7/6409 , C12P7/6427 , C12P7/6436 , C12P7/6463 , C12P7/6472 , C12P7/649 , C12P13/04 , C12R1/01 , C12Y103/03006 , Y02E50/13 , Y02E50/17 , Y02E50/18 , Y02E50/343
摘要： The present disclosure identifies pathways and mechanisms to confer production of carbon-based products of interest such as ethanol, ethylene, chemicals, polymers, n-alkanes, isoprenoids, pharmaceutical products or intermediates thereof in photoautotrophic organisms such that these organisms efficiently convert carbon dioxide and light into carbon-based products of interest, and in particular the use of such organisms for the commercial production of ethanol, ethylene, chemicals, polymers, n-alkanes, isoprenoids, pharmaceutical products or intermediates thereof.
摘要翻译： 本发明公开了确定感兴趣的碳基产物如乙醇，乙烯，化学品，聚合物，正烷烃，类异戊二烯，药物产品或其中间体在光自养生物体中生产的途径和机制，使得这些生物有效地转化二氧化碳和 特别是利用这些有机物用于商业生产乙醇，乙烯，化学品，聚合物，正烷烃，类异戊二烯，药物产品或其中间体。
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9.发明申请Methods and Compositions for the Recombinant Biosynthesis of Fatty Acids and Esters 失效脂肪酸和酯的重组生物合成的方法和组合物公开(公告)号：US20120009636A1
公开(公告)日：2012-01-12
申请号：US13243165
申请日：2011-09-23
申请人： David Arthur Berry , Noubar Boghos Afeyan , Frank Anthony Skraly , Christian Perry Ridley , Dan Eric Robertson , Regina Wilpiszeski , Martha Sholl
发明人： David Arthur Berry , Noubar Boghos Afeyan , Frank Anthony Skraly , Christian Perry Ridley , Dan Eric Robertson , Regina Wilpiszeski , Martha Sholl
IPC分类号： C12P7/62
CPC分类号： C10L1/026 , C10L2200/0476 , C10L2270/026 , C12N15/79 , C12P7/649 , C12Y602/01003 , Y02E50/13 , Y02E50/17
摘要： The present disclosure identifies methods and compositions for modifying photoautotrophic organisms, such that the organisms efficiently convert carbon dioxide and light into compounds such as esters and fatty acids. In certain embodiments, the compounds produced are secreted into the medium used to culture the organisms.
摘要翻译： 本公开内容确定了修饰光自养生物的方法和组合物，使得生物有效地将二氧化碳和光转化为化合物如酯和脂肪酸。 在某些实施方案中，所生产的化合物分泌到用于培养生物体的培养基中。
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10.发明申请Methods and Compositions for the Extracellular Transport of Biosynthetic Hydrocarbons and Other Molecules 审中-公开生物合成碳氢化合物和其他分子的细胞外运输的方法和组成公开(公告)号：US20120135486A1
公开(公告)日：2012-05-31
申请号：US13232961
申请日：2011-09-14
CPC分类号： C07K14/195 , C07K14/245 , C07K2319/03 , C07K2319/033 , C12N9/0004 , C12N9/0006 , C12N15/74 , C12P5/02 , C12P5/026
摘要： The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently convert carbon dioxide and light into hydrocarbons, e.g., n-alkanes and n-alkenes, wherein the n-alkanes are secreted into the culture medium via recombinantly expressed transporter proteins. In particular, the use of such organisms for the commercial production of n-alkanes and related molecules is contemplated.
摘要翻译： 本公开确定用于将光自养生物修饰为宿主的方法和组合物，使得生物有效地将二氧化碳和光转化成烃，例如正烷烃和正烯烃，其中正烷烃通过重组方式分泌到培养基中 表达转运蛋白。 特别地，考虑使用这种生物来商业生产正烷烃和相关分子。
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