Sorbitol oxidase from Xanthomonas maltophilia FERM BP-4512
    5.
    发明授权
    Sorbitol oxidase from Xanthomonas maltophilia FERM BP-4512 失效
    来自黄单孢杆菌FERM BP-4512的山梨醇氧化酶

    公开(公告)号:US5472862A

    公开(公告)日:1995-12-05

    申请号:US162628

    申请日:1993-12-07

    摘要: An isolated sorbitol oxidase from Xanthomonas maltophilia FERM BP-4512 is disclosed. The oxidase catalyzes the reaction of D-sorbitol+O.sub.2 .fwdarw.D-glucose+H.sub.2 O.sub.2, has a substrate specificity for D-sorbitol, D-mannitol, D-xylitol, and D-arabitol, has an optimum pH of 6.5 to 7.5, and has a molecular weight of 54 kD as determined by gel filtration or 43 kD as determined by SDS-PAGE. The enzyme can be used for measuring a polyol in a sample and as a reagent in a kit used for determining the presence of D-sorbitol.

    摘要翻译: 公开了一种分离出的来自黄单孢菌属FERM BP-4512的山梨醇氧化酶。 氧化酶催化D-山梨醇+ O2-> D-葡萄糖+ H2O2的反应,对D-山梨糖醇,D-甘露糖醇,D-木糖醇和D-阿拉伯糖醇具有底物特异性,最适pH为6.5〜7.5, 并且通过凝胶过滤测定的分子量为54kD,通过SDS-PAGE测定为43kD。 该酶可用于测量样品中的多元醇和用作测定D-山梨糖醇的存在的试剂盒中的试剂。

    Production of bacterial polysaccharides
    7.
    发明授权
    Production of bacterial polysaccharides 失效
    生产细菌多糖

    公开(公告)号:US4696900A

    公开(公告)日:1987-09-29

    申请号:US617743

    申请日:1984-06-06

    IPC分类号: C12P19/06 C12R1/64

    摘要: Polysaccharides are produced by single stage continuous culture of Xanthomonas bacteria, especially of the Xanthomonas campestris group in a chemically-defined culture medium. Cultures have been run for over 2,000 hours without reduction in the polysaccharide yield. The physical and chemical properties of the product can be controlled by selection of the growth limiting substrate (limiting nutrilite) in the culture medium to give a range of polysaccharides suitable for various applications.

    摘要翻译: 多糖通过在化学上定义的培养基中的黄单胞菌属细菌,特别是野油菜黄单胞菌(Xanthomonas campestris)的单阶段连续培养产生。 培养已经运行了超过2000小时,而不降低多糖产量。 可以通过选择培养基中生长限制底物(限制性营养素)来控制产物的物理和化学性质,以得到适用于各种应用的一系列多糖。

    Process for isolating microbial polysaccharides from their amine adducts
    8.
    发明授权
    Process for isolating microbial polysaccharides from their amine adducts 失效
    从其胺加合物中分离微生物多糖的方法

    公开(公告)号:US4562252A

    公开(公告)日:1985-12-31

    申请号:US522611

    申请日:1983-08-12

    CPC分类号: C08B37/0003 C12P19/06

    摘要: Microbial polysaccharides can be liberated from their amine adducts by treating them with ammonia or a highly volatile amine in the presence of monohydric alkanols having 1 to 3 carbon atoms. It is advantageous to use the amine adduct in the form of a moist press cake in which it is isolated from fermenter broths. The free polysaccharides are obtained in a virtually quantitative yield.

    摘要翻译: 通过在具有1至3个碳原子的一元链烷醇的存在下用氨或高挥发性胺处理微生物多糖,可以从它们的胺加合物中释放。 使用湿式压饼形式的胺加合物是有利的,其中它与发酵罐肉汤分离。 以几乎定量的产率获得游离多糖。

    Method for improving specific xanthan productivity during continuous
fermentation
    10.
    发明授权
    Method for improving specific xanthan productivity during continuous fermentation 失效
    在连续发酵过程中提高特定黄原胶生产率的方法

    公开(公告)号:US4311796A

    公开(公告)日:1982-01-19

    申请号:US168884

    申请日:1980-07-14

    IPC分类号: C12P19/06 C12R1/64

    摘要: In the production of xanthan gum by the action of Xanthomonas bacteria on a nutrient medium, the specific productivity of the organism employed can be improved by the increase in average cell concentration through the stepwise increase of growth limiting nutrients in the medium.

    摘要翻译: 在通过黄单胞菌属细菌在营养培养基上的作用生产黄原胶时,可以通过培养基中生长限制营养物的逐步增加的平均细胞浓度的增加来改善所用生物体的比生产力。