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    Process for preparing pectin from plant tissues
    2.
    发明授权
    Process for preparing pectin from plant tissues 失效
    从植物组织制备果胶的方法

    公开(公告)号：US4686187A

    公开(公告)日：1987-08-11

    申请号：US253929

    申请日：1981-04-03

    申请人： Takuo Sakai ,  Tohoru Katsuragi

    发明人： Takuo Sakai ,  Tohoru Katsuragi

    IPC分类号： C08B37/06 ,  C12P19/04 ,  A61F9/08

    CPC分类号： C08B37/0045 ,  C12R1/645 ,  C12R1/72 ,  C12R1/78 ,  C12R1/84 ,  C12R1/85 ,  C12R1/88 ,  Y10S435/911

    摘要： A process for preparing pectin, which is useful for foods and medicines or the like, by subjecting a plant tissue containing pectic substances to the action of a microorganism which belongs to the genus Endomyces, Endomycopsis, Saccharomyces, Shizosaccharomyces, Pichia, Hansenula, Debaryomyces, Hanseniaspora, Torulopsis, Candida or Kluyveromyces and possesses an activity liberating pectin from a plant tissue; or of a culture broth or processed material thereof to liberate pectin from said plant tissue; and recovering pectin.

    摘要翻译： PCT No.PCT / JP80 / 00179 Sec。 371日期：1981年4月3日 102（e）日期1981年4月3日PCT提交1980年8月8日PCT公布。 公开号WO81 / 00417 日期：1981年2月19日。一种制备果胶的方法，其用于食品和药物等，通过使含有果胶物质的植物组织受到属于内分泌物，Endomycopsis，Saccharomyces，Shizosaccharomyces属的微生物的作用 ，毕赤酵母，汉逊酵母，德巴利酵母，汉逊酵母，Torulopsis，假丝酵母​​或克鲁维酵母，并具有从植物组织释放果胶的活性; 或培养液或其加工物质以从所述植物组织释放果胶; 并回收果胶。

    Hansenula polymorpha mutants and process for the preparation of recombinant proteins using the same
    3.
    发明申请
    Hansenula polymorpha mutants and process for the preparation of recombinant proteins using the same 失效
    汉逊酵母多形汉逊酵母突变体及其制备方法

    公开(公告)号：US20020150983A1

    公开(公告)日：2002-10-17

    申请号：US09993192

    申请日：2001-11-14

    发明人： Sangki Rhee ,  Euisung Choi ,  Hyunah Kang ,  Junghoon Sohn ,  Junghoon Bae ,  Moowoong Kim ,  Michael Agaphonov ,  Myungkuk Kim

    IPC分类号： C12P021/02 ,  C12N001/21 ,  C12N009/50

    CPC分类号： C12N15/815 ,  C12N9/48 ,  C12N15/80 ,  C12R1/78

    摘要： Disclosed are Hansenula polymorpha mutants useful as host cells through which various proteins can be produced as being intact at high yield and a process for preparing recombinant proteins using the host cells. Using various vectors, Hansenula polymorpha is made to be a mutant which is deprived of methanol assimilating ability and incapable of utilizing methanol as a carbon source. This Hansenula polymorpha mutant is used as a high yield host to produce recombinant proteins without continuous feeding of methanol, with the aid of an expression cassette carrying a promoter capable of inducing the expression at a low concentration of methanol. Further, the mutant is also lacking in carboxypeptidase Y, protease Y and/or carboxypeptidase a activity, so the recombinant protein of interest is not degraded at its carboxyl terminal when being expressed in the cell. Thus, intact recombinant protein can be obtained. Also, there is disclosed a pop-out technique in which a recombinant protein expression cassette is inserted into a MOX gene site of the mutant and is allowed to pop out therefrom, thereby utilizing the mutant as a host for general use in producing various proteins of interest

    摘要翻译： 公开了可用作宿主细胞的汉逊酵母多形汉逊酵母突变体，通过该宿主细胞可以以高产量产生各种蛋白质完整的蛋白质，以及使用宿主细胞制备重组蛋白质的方法。 使用各种载体，多形汉逊酵母被认为是一种被剥夺了甲醇同化能力并且不能利用甲醇作为碳源的突变体。 这种汉逊酵母多形突变体被用作高产量宿主以产生重组蛋白，而不用连续喂入甲醇，借助于携带能够以低浓度甲醇诱导表达的启动子的表达盒。 此外，突变体也缺乏羧肽酶Y，蛋白酶Y和/或羧肽酶a活性，所以当在细胞中表达时，其目的重组蛋白质在其羧基末端不被降解。 因此，可以获得完整的重组蛋白。 此外，公开了一种突变技术，其中将重组蛋白表达盒插入到突变体的MOX基因位点，并从其中突出出来，从而利用突变体作为一般用于产生各种蛋白质的各种蛋白质的宿主 利益

    Hansenula polymorpha mutants and process for the preparation of recombinant proteins using the same
    7.
    发明授权
    Hansenula polymorpha mutants and process for the preparation of recombinant proteins using the same 失效
    汉逊酵母多形汉逊酵母突变体及其制备方法

    公开(公告)号：US06838555B2

    公开(公告)日：2005-01-04

    申请号：US09993192

    申请日：2001-11-14

    申请人： Sangki Rhee ,  Euisung Choi ,  Hyunah Kang ,  Junghoon Sohn ,  Junghoon Bae ,  Moowoong Kim ,  Michael Agaphonov ,  Myungkuk Kim

    发明人： Sangki Rhee ,  Euisung Choi ,  Hyunah Kang ,  Junghoon Sohn ,  Junghoon Bae ,  Moowoong Kim ,  Michael Agaphonov ,  Myungkuk Kim

    IPC分类号： C12N9/48 ,  C12N15/80 ,  C12N15/81 ,  C07H21/04

    CPC分类号： C12N15/815 ,  C12N9/48 ,  C12N15/80 ,  C12R1/78

    摘要： Disclosed are Hansenula polymorpha mutants useful as host cells through which various proteins can be produced as being intact at high yield and a process for preparing recombinant proteins using the host cells. Using various vectors, Hansenula polymorpha is made to be a mutant which is deprived of methanol assimilating ability and incapable of utilizing methanol as a carbon source. This Hansenula polymorpha mutant is used as a high yield host to produce recombinant proteins without continuous feeding of methanol, with the aid of an expression cassette carrying a promoter capable of inducing the expression at a low concentration of methanol. Further, the mutant is also lacking in carboxypeptidase Y, protease Y and/or carboxypeptidase a activity, so the recombinant protein of interest is not degraded at its carboxyl terminal when being expressed in the cell. Thus, intact recombinant protein can be obtained. Also, there is disclosed a pop-out technique in which a recombinant protein expression cassette is inserted into a MOX gene site of the mutant and is allowed to pop out therefrom, thereby utilizing the mutant as a host for general use in producing various proteins of interest.

    摘要翻译： 公开了可用作宿主细胞的汉逊酵母多形汉逊酵母突变体，通过该宿主细胞可以以高产量产生各种蛋白质完整的蛋白质，以及使用宿主细胞制备重组蛋白质的方法。 使用各种载体，多形汉逊酵母被认为是一种被剥夺了甲醇同化能力并且不能利用甲醇作为碳源的突变体。 这种汉逊酵母多形突变体被用作高产量宿主以产生重组蛋白，而不用连续喂入甲醇，借助于携带能够以低浓度甲醇诱导表达的启动子的表达盒。 此外，突变体也缺乏羧肽酶Y，蛋白酶Y和/或羧肽酶a活性，所以当在细胞中表达时，其目的重组蛋白质在其羧基末端不被降解。 因此，可以获得完整的重组蛋白。 此外，公开了一种突变技术，其中将重组蛋白表达盒插入到突变体的MOX基因位点，并从其中突出出来，从而利用突变体作为一般用于产生各种蛋白质的各种蛋白质的宿主 利益。

    Strain of Hansenulla californica yeast used for the decomposition of polychlorinated biphenyls
    8.
    发明授权
    Strain of Hansenulla californica yeast used for the decomposition of polychlorinated biphenyls 有权
    用于分解多氯联苯的汉逊酵母菌的酵母菌株

    公开(公告)号：US06284521B1

    公开(公告)日：2001-09-04

    申请号：US09601509

    申请日：2000-09-20

    申请人： Roman Vladimirovich Borovick ,  Gennady Alekseevich Zharikov ,  Vladimir Vladimirovich Kapranov

    发明人： Roman Vladimirovich Borovick ,  Gennady Alekseevich Zharikov ,  Vladimir Vladimirovich Kapranov

    IPC分类号： C12N114

    CPC分类号： C12R1/78 ,  B09C1/10 ,  C02F3/34 ,  C02F2101/363

    摘要： Hansenulla californica yeast strain VKPM Y-2284 is capable of degrading polychlorinated biphenyls (PCBs). The strain may be employed to detoxicate environment media and PCB-containing industrial wastes. To produce biomass, the strain is incubated on media which contain carbon sources, nitrogen sources and mineral salts. The strain is cultivated by a subsurface method up to a titer from 5.0·106 to 1.0×107 cells per cu cm. The produced biomass is employed for degrading PCBs in concentrations from 106 to 105 cells per cu cm. The strain ensures from 30 to 50% reduction in PCB content in soil and water.

    摘要翻译： 汉逊酵母菌株VKPM Y-2284能够降解多氯联苯（PCBs）。 该菌株可用于对环境介质和含PCB工业废物进行排毒。 为了生产生物质，将菌株在含有碳源，氮源和矿物盐的培养基上孵育。 通过地下方法培养菌株，直至每立方厘米5.0.106至1.0×10 7个细胞的滴度。 生产的生物质用于降解PCBs，浓度为每立方厘米106至105个细胞。 该应变确保土壤和水中PCB含量降低30％至50％。

    Process for the production of pyruvic acid and citric acid
    9.
    发明授权
    Process for the production of pyruvic acid and citric acid 失效
    生产丙酮酸和柠檬酸的方法

    公开(公告)号：US4326030A

    公开(公告)日：1982-04-20

    申请号：US100395

    申请日：1979-12-05

    申请人： Refaat M. El-Sayed

    发明人： Refaat M. El-Sayed

    IPC分类号： C12P7/40 ,  C12P7/48

    CPC分类号： C12R1/78 ,  C12P7/40 ,  C12P7/48 ,  C12R1/19 ,  Y10S435/849 ,  Y10S435/93

    摘要： Process for the production of citric acid, characterized in that E. Coli KG 93, F.sup.- is cultivated in a first step for 15-24 hours at a temperature of 20.degree.-37.degree. C. and a pH of 5.0-7.5 on a substrate consisting of whey permeate to which has been added phosphates in a content of 0.8-1.6 g/l and nitrates in a content of 0.8-1.2 g/l or a corresponding quantity of urea, that H. Wickerhamii CBS 4308 in a second step is cultivated for 20-26 hours at a temperature of 15.degree.-35.degree. C. and a pH of 4.5-6.5 on the cultivating solution from the first step, whereupon citric acid is obtained from the cultivating solution in a way known per se.

    摘要翻译： 制备柠檬酸的方法，其特征在于，大肠杆菌KG 93，F-在20〜37℃的温度下在第一步中培养15-24小时，pH为5.0-7.5 由其中添加了0.8-1.6g / l的磷酸盐的乳清渗透物和含有0.8-1.2g / l的硝酸盐或相应量的尿素组成的底物，在第二步中H.Wickerhamii CBS 4308 在第一步的培养液中在15〜35℃的温度下培养20-26小时，pH为4.5-6.5，从而以本身已知的方式从培养液中获得柠檬酸。