公开(公告)号：US20130323794A1

公开(公告)日：2013-12-05

申请号：US13891685

申请日：2013-05-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： James HARTLEY ,  Michael Brasch

IPC: C12P19/34

CPC classification number: C12P19/34 ,  C12N9/00 ,  C12N15/10 ,  C12N15/64 ,  C12N15/66

Abstract: Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s).

公开(公告)号：US20150093787A1

公开(公告)日：2015-04-02

申请号：US14452517

申请日：2014-08-05

Applicant: Life Technologies Corporation

Inventor： James HARTLEY ,  Michael Brasch ,  Gary Temple ,  David Cheo

IPC: C12N15/11

CPC classification number: C12N15/11 ,  C12N15/10 ,  C12N15/63 ,  C12N2800/70

Abstract: The present invention relates generally to compositions and methods for use in recombinational cloning of nucleic acid molecules. In particular, the invention relates to nucleic acid molecules encoding one or more recombination sites or portions thereof, to nucleic acid molecules comprising one or more of these recombination site nucleotide sequences and optionally comprising one or more additional physical or functional nucleotide sequences. The invention also relates to vectors comprising the nucleic acid molecules of the invention, to host cells comprising the vectors or nucleic acid molecules of the invention, to methods of producing polypeptides using the nucleic acid molecules of the invention, and to polypeptides encoded by these nucleic acid molecules or produced by the methods of the invention. The invention also relates to antibodies that bind to one or more polypeptides of the invention or epitopes thereof. The invention also relates to the use of these compositions in methods for recombinational cloning of nucleic acids, in vitro and in vivo, to provide chimeric DNA molecules that have particular characteristics and/or DNA segments.

Abstract translation: 本发明一般涉及用于核酸分子重组克隆的组合物和方法。 特别地，本发明涉及编码一个或多个重组位点或其部分的核酸分子与包含这些重组位点核苷酸序列中的一个或多个并且任选地包含一个或多个另外的物理或功能核苷酸序列的核酸分子。 本发明还涉及包含本发明的核酸分子的载体，包含本发明的载体或核酸分子的宿主细胞，使用本发明的核酸分子产生多肽的方法，以及由这些核酸编码的多肽 酸分子或通过本发明的方法制备。 本发明还涉及结合本发明的一种或多种多肽或其表位的抗体。 本发明还涉及这些组合物用于在体外和体内重组克隆核酸以提供具有特定特征和/或DNA片段的嵌合DNA分子的方法中的用途。

公开(公告)号：US20140004567A1

公开(公告)日：2014-01-02

申请号：US13931804

申请日：2013-06-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： James HARTLEY ,  Michael Brasch ,  Gary Temple ,  Donna Fox

IPC: C12N15/64

CPC classification number: C12N15/64 ,  C12N9/00 ,  C12N15/10 ,  C12N15/66

Abstract: Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s).

Abstract translation: 通过在体外和体内使用核酸，载体和方法，使用工程重组位点和重组蛋白来移动或交换DNA分子片段来提供重组克隆，以提供具有所需特征的嵌合DNA分子和 /或DNA片段。

公开(公告)号：US20140206577A1

公开(公告)日：2014-07-24

申请号：US14066574

申请日：2013-10-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Devon YOUNG ,  James HARTLEY ,  Alice YOUNG

IPC: C12N15/63 ,  C12Q1/68

CPC classification number: C12N15/63 ,  C07K14/245 ,  C07K14/255 ,  C07K14/32 ,  C12N15/10 ,  C12N15/1096 ,  C12N15/64 ,  C12N15/66 ,  C12N15/70 ,  C12N15/87 ,  C12Q1/6834

Abstract: The present invention provides materials and methods for the utilization of the specific interaction of replication termination sequences with their binding proteins in molecular biology applications.

Abstract translation: 本发明提供了在分子生物学应用中利用复制终止序列与其结合蛋白的特异性相互作用的材料和方法。