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    Venezuelan equine encephalitis virus replicons with adaptive mutations in the genome and uses thereof
    1.
    发明申请
    Venezuelan equine encephalitis virus replicons with adaptive mutations in the genome and uses thereof 有权
    委内瑞兰马脑炎病毒复制子与基因组中的适应性突变及其用途

    公开(公告)号：US20060251678A1

    公开(公告)日：2006-11-09

    申请号：US11227671

    申请日：2005-09-14

    申请人： Ilya Frolov ,  Elena Frolova

    发明人： Ilya Frolov ,  Elena Frolova

    IPC分类号： A61K39/395 ,  A61K39/12

    CPC分类号： C12N7/00 ,  C12N2770/36121 ,  C12N2770/36143

    摘要： The present invention provides a Venezuelan equine encephalitis virus replicon RNA useful in the development of stable lines of mammalian, avian and insect cells in which these replicons will persistently replicate. Venezuelan equine encephalitis (VEE) virus replicons contain a number of unique adaptive mutations that make the replicons noncytopathic. The replicons remain resistant to IFN-α/β. Replicon replication leads to high-level production of heterologous proteins, which are encoded by the replicons' genome and are under the control of a viral subgenomic promoter. Also provided are methods of screening for inhibitory compounds of Venezuelan equine encephalitis virus replication and eastern equine encephalitis virus replication.

    摘要翻译： 本发明提供了一种可用于发展哺乳动物，禽和昆虫细胞的稳定系的委内瑞兰马脑炎病毒复制子RNA，其中这些复制子将持续复制。 委内瑞兰马脑炎（VEE）病毒复制子包含许多独特的适应性突变，使复制子非细胞病变。 复制子对IFN-α/β仍然具有抗性。 复制子复制导致异源蛋白质的高水平生产，其由复制子基因组编码并在病毒亚基因组启动子的控制之下。 还提供了筛选委内瑞兰马脑炎病毒复制和东马脑炎病毒复制的抑制性化合物的方法。

    Venezuelan equine encephalitis virus replicons with adaptive mutations in the genome and uses thereof
    7.
    发明授权
    Venezuelan equine encephalitis virus replicons with adaptive mutations in the genome and uses thereof 有权
    委内瑞兰马脑炎病毒复制子与基因组中的适应性突变及其用途

    公开(公告)号：US07332322B2

    公开(公告)日：2008-02-19

    申请号：US11227671

    申请日：2005-09-14

    申请人： Ilya Frolov ,  Elena Frolova

    发明人： Ilya Frolov ,  Elena Frolova

    IPC分类号： C12N7/04 ,  C12N15/86 ,  C12P21/04 ,  C12P19/34 ,  C12Q1/68

    CPC分类号： C12N7/00 ,  C12N2770/36121 ,  C12N2770/36143

    摘要： The present invention provides a Venezuelan equine encephalitis virus replicon RNA useful in the development of stable lines of mammalian, avian and insect cells in which these replicons will persistently replicate. Venezuelan equine encephalitis (VEE) virus replicons contain a number of unique adaptive mutations that make the replicons noncytopathic. The replicons remain resistant to IFN-α/β. Replicon replication leads to high-level production of heterologous proteins, which are encoded by the replicons' genome and are under the control of a viral subgenomic promoter. Also provided are methods of screening for inhibitory compounds of Venezuelan equine encephalitis virus replication and eastern equine encephalitis virus replication.

    摘要翻译： 本发明提供了一种可用于发展哺乳动物，禽和昆虫细胞的稳定系的委内瑞兰马脑炎病毒复制子RNA，其中这些复制子将持续复制。 委内瑞兰马脑炎（VEE）病毒复制子包含许多独特的适应性突变，使复制子非细胞病变。 复制子对IFN-α/β仍然具有抗性。 复制子复制导致异源蛋白质的高水平生产，其由复制子基因组编码并在病毒亚基因组启动子的控制下。 还提供了筛选委内瑞兰马脑炎病毒复制和东马脑炎病毒复制的抑制性化合物的方法。

    Pseudoinfectious flavivirus and uses thereof
    10.
    发明申请
    Pseudoinfectious flavivirus and uses thereof 有权
    伪感染黄病毒及其用途

    公开(公告)号：US20090155301A1

    公开(公告)日：2009-06-18

    申请号：US11711532

    申请日：2007-02-27

    申请人： Peter W. Mason ,  Elena Frolova ,  Ilya Frolov

    发明人： Peter W. Mason ,  Elena Frolova ,  Ilya Frolov

    IPC分类号： A61K39/12 ,  C12N7/00 ,  C12N5/06 ,  C12N15/87 ,  A61K35/76

    CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K2039/5254 ,  C12N2770/24134 ,  C12N2770/24162 ,  Y02A50/386 ,  Y02A50/388 ,  Y02A50/39 ,  Y02A50/394 ,  Y02A50/396

    摘要： The present invention discloses a replication-deficient pseudoinfective virus belonging to the Flaviviridae family that lack the capsid gene, where the replication-deficient pseudoinfective virus propagates only in cells expressing the capsid or capsid, prM and envelope protein of the flavivirus. The present also discloses the method of producing such viruses on a large scale and the use of these pseudoinfective viruses as vaccines for preventing diseases caused by infections of humans or animals by the viruses belonging to this family.

    摘要翻译： 本发明公开了属于黄病毒科的复制缺陷型假性感染病毒，其缺乏衣壳基因，其中复制缺陷型假病毒病毒仅在表达黄病毒的衣壳或衣壳，prM和包膜蛋白的细胞中繁殖。 本发明还公开了大规模生产这种病毒的方法，以及这些假感染性病毒作为疫苗用于预防由该家族的病毒感染人或动物引起的疾病的疫苗。