公开(公告)号：US20250067671A1

公开(公告)日：2025-02-27

申请号：US18807328

申请日：2024-08-16

Applicant: Tianma Japan, Ltd.

Inventor： Ken SUMIYOSHI ,  Ayuko IMAI

IPC: G01N21/64

Abstract: A fluorescence polarization measurement device includes a light source unit, a polarization direction modulation element, a detector, and a controller. The polarization direction modulation element spatially modulates a polarization direction of the excitation light by a predetermined frequency, and emits, on a measurement target solution, the excitation light for which the polarization direction is spatially modulated by the predetermined frequency. The detector detects a spatial distribution of fluorescence intensity of the fluorescence having a polarization direction in a predetermined direction from among fluorescence emitted from the measurement target solution due to the excitation light. The controller extracts, from the detected spatial distribution of fluorescence intensity, a direct current component and a component having a frequency identical to the predetermined frequency, and calculates, based on the extracted component and the direct current component, a degree of polarization of the measurement target solution.

公开(公告)号：US20250066704A1

公开(公告)日：2025-02-27

申请号：US18812562

申请日：2024-08-22

Applicant: CORNELL UNIVERSITY

Inventor： Alireza ABBASPOURRAD ,  Ann NGUYEN ,  Mohammad YAGHOOBI ,  Morteza AZIZI

IPC: C12M3/06 ,  C12M1/00 ,  C12M1/02 ,  C12M1/34 ,  C12Q1/18 ,  G01N21/64

Abstract: The present disclosure relates to a microfluidic circuit comprising a drug inlet port; an outlet port; a drug inlet main channel fluidically connecting the drug inlet port and the outlet port, where said drug inlet main channel comprises (i) a plurality of serpentine mixers and (ii) a plurality of dead-end first microchamber sets; a negative inlet port; a negative inlet main channel fluidically connecting the negative inlet port and the drug inlet main channel; a plurality (n) of ladder channels, where each of the plurality (n) of the ladder channels is fluidically connected to both the drug inlet main channel and the negative inlet main channel; and an outlet channel fluidically connected to the drug inlet main channel between the drug inlet port and the outlet port. Also disclosed is a microfluidic device comprising a microfluidic circuit of the present disclosure and a method for performing an assay.

公开(公告)号：US20250066403A1

公开(公告)日：2025-02-27

申请号：US18814583

申请日：2024-08-25

Applicant: Hangzhou Normal University

Inventor： Shouchun YIN ,  Yang LI ,  Xiongjie LIN ,  Tian HE

IPC: C07F15/00 ,  C07F5/02 ,  C09K11/06 ,  G01N21/64

Abstract: Disclosed are a linear boron-dipyrromethene electron donor having a 180° coordination included angle and a supramolecular metallacycle, and synthesis processes therefor and applications thereof. The invention provides a linear boron-dipyrromethene electron donor as shown in formula 3 or formula 4, and a boron-dipyrromethene ligand-based supramolecular coordination compound having a 180° coordination included angle which is prepared by the metal coordination of the linear boron-dipyrromethene electron donor. Compared with a prepolymer, the boron-dipyrromethene ligand-based supramolecular coordination compound has better cell passive uptake capacity, cell fluorescence imaging capacity and photosensitive (singlet oxygen generation) capability.

公开(公告)号：US20250064309A1

公开(公告)日：2025-02-27

申请号：US18943587

申请日：2024-11-11

Applicant: The Board of Trustees of the Leland Stanford Junior University ,  Howard Hughes Medical Institute

Inventor： Simon Haziza ,  Radoslaw Chrapkiewicz ,  Mark J. Schnitzer

IPC: A61B1/04 ,  G01N21/64

Abstract: A method for concurrently measuring multiple biological parameters in an animal includes: a) illuminating using multiple illumination sources a region-of-interest of the animal that expresses a first genetically encoded fluorescent indicators (GEFI), a second GEFI, and a long-Stokes-shift (LSS) fluorescent compound that is insensitive to the multiple biological parameters; b) concurrently detecting fluorescence signals from the first GEFI, the second GEFI, and the LSS fluorescent compound using a multi-channel fluorescence sensing optical system; and c) processing the detected fluorescence signals to determine values of the multiple biological parameters, wherein the processing reduces instrument and/or biological artifacts in the values of the multiple biological parameters. The first and second GEFIs have distinct absorption spectra, where the first GEFI absorption spectrum overlaps the LSS fluorescent compound absorption spectrum, and the second GEFI emission spectrum overlaps the emission spectrum of the LSS fluorescent compound.

公开(公告)号：US12235463B2

公开(公告)日：2025-02-25

申请号：US17566765

申请日：2021-12-31

Applicant: Quantum-Si Incorporated

Inventor： Jonathan M. Rothberg ,  Paul E. Glenn ,  Jonathan C. Schultz ,  Benjamin Cipriany

IPC: G02B27/09 ,  G02B6/34 ,  G02B7/00 ,  G02B19/00 ,  G01N21/64

Abstract: Apparatus and methods for coupling an optical beam from an optical source to a hi-tech system are described. A compact, low-cost beam-shaping and steering assembly may be located between the optical source and hi-tech system and provide automated adjustments to beam parameters such as beam position, beam rotation, and beam incident angles. The beam-shaping and steering assembly may be used to couple an elongated beam to a plurality of optical waveguides.

公开(公告)号：US12235263B2

公开(公告)日：2025-02-25

申请号：US16999138

申请日：2020-08-21

Applicant: FUJIFILM Corporation

Inventor： Hiroyuki Chiku ,  Takeru Shigemura ,  Kousuke Watanabe ,  Yoshinori Kanazawa ,  Kouitsu Sasaki ,  Kazuhei Kaneko

IPC: G01N33/543 ,  G01N21/64 ,  G01N33/547

Abstract: An object of the present invention is to provide a kit, a method, and a reagent which prevent the problem of false positive due to nonspecific adsorption, suppress the increase in noise to be generated, and are capable of achieving high-precision measurement of progesterone in a wide concentration range from a low concentration to a high concentration, in the measurement of progesterone in a biological sample. The present invention provides a progesterone measuring kit including a first particle having a label and modified with a first binding substance, a second particle having no label and modified with a second binding substance, a flow channel, and a substrate, in which the first particle contains a compound represented by Formula (1) and a particle, and an average particle diameter of the first particles is 50 to 250 nm, an average particle diameter of the second particles is 70 to 500 nm, and the average particle diameter of the second particles is larger than the average particle diameter of the first particles. Each symbol in the formula has the meaning described in the present specification.

公开(公告)号：US12235216B2

公开(公告)日：2025-02-25

申请号：US17798786

申请日：2020-02-12

Applicant: MGI Tech Co., Ltd.

Inventor： Heming Jiang ,  Yi Huang ,  Qian Deng ,  Bin Yang ,  Xin Wen ,  Mingyou Cao ,  Yanqiao Huang ,  Ziyi Wu ,  Craig Edward Uhrich ,  Brian Keith Hauser ,  Chintang Yen

IPC: G01N21/64

Abstract: An optical imaging system (1) is configured for photographing a sample and includes a lighting module (11) and an imaging module (15). The lighting module (11) is configured for outputting excitation light, the excitation light is configured to excite the sample to generate excited light, the imaging module (15) comprises a time delay integration line scan camera (151), the time delay integration line scan camera (151) is configured to record the excited light. A biochemical substance detection system using the optical imaging system (1) is also provided, improving the detection flux.

公开(公告)号：US12232706B2

公开(公告)日：2025-02-25

申请号：US16908640

申请日：2020-06-22

Applicant: Stryker Corporation

Inventor： Ajay Ramesh ,  Perry Hu ,  Harman Singh ,  Chien Mien Pang ,  Vasudev Nambakam

IPC: A61B1/06 ,  A61B1/00 ,  A61B1/04 ,  A61B1/045 ,  A61B1/07 ,  G01N21/64 ,  H04N9/73 ,  H04N23/11 ,  H05B45/22 ,  H04N23/50

Abstract: A light source includes a first LED in communication with a first DAC; a second LED in communication with a second DAC; a third LED in communication with a third DAC; a color sensor capable of sensing light in the visible light spectrum; and one or more processors configured for: receiving color intensity values from the color sensor, determining whether the color intensity values are balanced to achieve white light, adjusting the current to the second DAC and third DAC until the color intensity levels are balanced to achieve white light at a combined intensity level, setting a new red LED current value, a new green LED current value, and a new blue LED current value to adjust the combined intensity level toward a target intensity level, and repeating the setting step until the target intensity level is achieved.

公开(公告)号：US20250060364A1

公开(公告)日：2025-02-20

申请号：US18938509

申请日：2024-11-06

Applicant: CANON KABUSHIKI KAISHA ,  CANON MEDICAL SYSTEMS CORPORATION

Inventor： NORISHIGE KAKEGAWA ,  TAKAHIRO MASUMURA ,  FUMIO YAMAUCHI ,  KENGO KANAZAKI ,  TOMOHIRO NAKAMURA ,  IKUO NAKAJIMA ,  TEIGO SAKAKIBARA

IPC: G01N33/543 ,  G01N21/64

Abstract: Provided are an analysis method and an analysis apparatus each of which enables the analysis of the concentration of a target substance in a wide concentration range and with high sensitivity based on polarization anisotropy. Specifically, provided is an analysis method including measuring a value (R) for polarization anisotropy through use of a luminescent reagent that binds to a target substance, to thereby calculate a concentration of the target substance, the analysis method including: a mixing step of mixing a sample containing the target substance and the luminescent reagent to provide a mixed liquid; and a measuring step of measuring the R of the mixed liquid after a lapse of a time T from a time of the mixing, wherein in the measuring step, a time at which the R reaches R1 is represented by T1, and the concentration of the target substance is calculated based on the T1.

公开(公告)号：US20250059593A1

公开(公告)日：2025-02-20

申请号：US18776428

申请日：2024-07-18

Applicant: ULTIMA GENOMICS, INC.

Inventor： Linda LEE ,  Steven MENCHEN ,  Theo NIKIFOROV ,  Gilad ALMOGY ,  Florian OBERSTRASS

IPC: C12Q1/6823 ,  C12Q1/6806 ,  C12Q1/6869 ,  G01N21/64

Abstract: The disclosure provides methods for sequencing nucleic acids using, including with nucleotide analogs and subsequently appended labels.