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    Sustained totipotent culture of selected monocot genera
    1.
    发明授权
    Sustained totipotent culture of selected monocot genera 有权
    所选单子叶植物的持续全能培养

    公开(公告)号：US06821782B2

    公开(公告)日：2004-11-23

    申请号：US10068584

    申请日：2002-02-05

    申请人： Laszlo Marton ,  Mihaly Czako

    发明人： Laszlo Marton ,  Mihaly Czako

    IPC分类号： C12N500

    CPC分类号： C12N15/8259 ,  A01H4/005 ,  A01H4/008 ,  B09C1/105 ,  C02F3/327 ,  C12N15/8201 ,  C12N15/8205 ,  Y02W10/18

    摘要： A method is provided for generating sustained totipotent tissue cultures of a plant of the Class Monocotyledonae, and for micropropagating such plant in vitro, wherein immature inflorescence are cultivated to produce totipotent tissue which is suitable for sustained maintenance and propagation. Greening of the tissue can be induced under light and the multishoot culture can multiply by microtillering. Foreign genes can be introduced into the tissue if desired, and the transgenic plants can be used in phytoremediation technologies in the field and in phytoreactors independently of seasons.

    摘要翻译： 提供了一种用于产生单子叶植物的植物的持续全能组织培养物的方法，并且用于在体外微量繁殖这种植物，其中栽培不成熟的花序以产生适于持续维持和繁殖的全能组织。 可以在光照条件下诱导组织的绿化，多层培养可以通过微量化繁殖。 如果需要，外来基因可以被引入到组织中，并且转基因植物可以独立于季节用于野外的植物修复技术和植物存储器中。

    Method for enhancing hematopoiesis
    2.
    发明授权
    Method for enhancing hematopoiesis 失效
    增强造血的方法

    公开(公告)号：US06821513B1

    公开(公告)日：2004-11-23

    申请号：US10019165

    申请日：2001-12-20

    申请人： William H. Fleming

    发明人： William H. Fleming

    IPC分类号： C12N500

    CPC分类号： A61K35/44

    摘要： A method is provided for enhancing hematopoiesis. The method includes transplanting at least a therapeutically effective portion of an isolated vascular tissue into a subject, wherein the vascular tissue enhances hematopoiesis. A method is also provided for detecting an agent that affects hematopoiesis. The method includes transplanting a portion of an isolated vascular tissue into a subject, wherein the portion of the isolated vascular tissue is sufficient to enhance hematopoiesis. The vascular tissue is treated with an agent, and hematopoiesis is detected in the subject. Hematopoiesis in the subject is compared with hematopoietic in a control. A change in hematopoiesis in the subject as compared to the control indicatives that the agent affects hematopoiesis. A method is provided for isolating a hematopoiesis growth factor using the method of the invention. In addition, a method is provided for isolating a hematopoietic stem cell using the method of the invention. Pharmaceutical compositions and kits for promoting hematopoiesis are also disclosed. In addition, a non-human animal model is also disclosed for testing agents that affect hematopoiesis. The model system is a non-human animal deficient for hematopoiesis transplanted with an isolated portion of a vascular tissue, wherein the portion of the vascular tissue is sufficient to promote hematopoiesis.

    摘要翻译： 提供了一种增强造血功能的方法。 该方法包括将至少一种分离的血管组织的治疗有效部分移植到受试者中，其中血管组织增强造血功能。 还提供了一种用于检测影响造血作用的药剂的方法。 该方法包括将一部分分离的血管组织移植到受试者中，其中分离的血管组织的部分足以增强造血功能。 用药剂处理血管组织，并在受试者中检测造血。 将对象中的血细胞生成与对照中的造血相比较。 与受试者影响造血作用的对照指标相比，受试者造血的变化。 提供了使用本发明的方法分离造血生长因子的方法。 此外，提供了使用本发明的方法分离造血干细胞的方法。 还公开了用于促进造血作用的药物组合物和试剂盒。 此外，还公开了一种影响造血作用的测试剂的非人动物模型。 模型系统是一种非人类动物，缺乏移植有分离部分血管组织的造血，其中血管组织的该部分足以促进造血。

    In vitro production of amoebocytes from tachypleus gigas in leibovitz culture medium
    3.
    发明授权
    In vitro production of amoebocytes from tachypleus gigas in leibovitz culture medium 失效
    在leibovitz培养基中体外生产来自tachypleus gigas的变形细胞

    公开(公告)号：US06790659B2

    公开(公告)日：2004-09-14

    申请号：US10109543

    申请日：2002-03-28

    申请人： Ramesh Ramchandra Bhonde ,  Anil Chatterji

    发明人： Ramesh Ramchandra Bhonde ,  Anil Chatterji

    IPC分类号： C12N500

    CPC分类号： C12N5/0601 ,  C12N2500/82

    摘要： The present invention provides a process for large scale in vitro production of amoebocytes of Indian Horseshoe Crab (Tachypleus gigas) (T. gigas) from dissected gill flaps of T. gigas, in Leibovitz L-15 culture medium concentration (2×), to provide enhanced generation of amoebocytes. The process comprises the steps of: dissecting gill flaps of T. gigas; washing the gill flaps with an antibiotic solution followed by alcohol; culturing the gill flaps in tissue culture plates of sterile saline on a Rocker platform; culturing further the gill flaps in Leibovitz L-15 culture medium (2×); purging the gill flaps with Tween 80 solution; and purging again the gill flaps with horseshoe crab serum, while keeping the gill flaps in the culture medium viable for 90 days by feeding with fresh medium at an interval of 10-15 days to enable the enhanced release of amoebocytes both within and outside the gill flaps.

    摘要翻译： 本发明提供了一种在Leibovitz L-15培养基浓度（2x）中大规模体外生产印度马蹄蟹（Tachypleus gigas）（Tigigus gigas）（T.gigas））来自解剖的T.gigas鳃片的变形细胞的方法，以提供 增强的变形细胞生成。 该过程包括以下步骤：解剖T.gigas的鳃片; 用抗生素溶液洗涤鳃瓣，然后用酒精洗涤; 在Rocker平台上的无菌盐水组织培养板中培养鳃瓣; 在Leibovitz L-15培养基（2x）中进一步培养鳃瓣; 用吐温80溶液清洗鳃瓣; 并用马蹄蟹血清再次清除鳃瓣，同时通过以10-15天的间隔喂养新鲜培养基将鳃瓣保持在培养基中90天，以使得能够在鳃内和外部增强释放的变形细胞 襟翼

    Process for continuous purification and concentration of leukocytes from blood
    6.
    发明授权
    Process for continuous purification and concentration of leukocytes from blood 失效
    从血液中连续纯化和浓缩白细胞的方法

    公开(公告)号：US06743624B1

    公开(公告)日：2004-06-01

    申请号：US09646985

    申请日：2000-11-17

    申请人： Mats Jarekrans

    发明人： Mats Jarekrans

    IPC分类号： C12N500

    CPC分类号： C12N5/0087

    摘要： Process and apparatus for the continuous purification and concentration of leukocytes from blood, characterized in that said process comprises the following steps: (a) seperating plasma from the blood by filtration in order to achieve a filtered buffy coat fraction; (b) adding an aqueous solution, which is hypotonic in relation to plasma, to the buffy coat fraction from step (a), in order to achieve lysation of erythrocytes contained in the buffy coat fraction; (c) mixing the buffy coat fraction and the aqueous hypotonic solution from step (b) in a mixing device; (d) leading the mixture from step (c) through a retention vessel; (e) leading the mixture from step (d) through a centrifuge in order to seperate the leukocytes; (f) collecting the separated leukocytes from step (e).

    摘要翻译： 用于从血液中连续纯化和浓缩白细胞的方法和装置，其特征在于所述方法包括以下步骤：（a）通过过滤从血液中分离血浆，以获得过滤的血沉棕黄层级分; （b）将与血浆相比低渗的水溶液与来自步骤（a）的血沉棕黄层组分相加，以实现沉积在血沉棕黄层中的红细胞溶解; （c）在混合装置中将来自步骤（b）的血沉棕黄层和低渗溶液混合; （d）使来自步骤（c）的混合物通过保留容器; （e）引导来自步骤（d）的混合物通过离心机以分离白细胞; （f）从步骤（e）收集分离的白细胞。

    Cytokine receptor and nucleic acids encoding the same
    7.
    发明授权
    Cytokine receptor and nucleic acids encoding the same 有权
    细胞因子受体和编码相同的核酸

    公开(公告)号：US06740520B2

    公开(公告)日：2004-05-25

    申请号：US09964994

    申请日：2001-09-26

    申请人： Audrey Goddard ,  Paul J. Godowski ,  Austin L. Gurney ,  Colin K. Watanabe ,  William I. Wood

    发明人： Audrey Goddard ,  Paul J. Godowski ,  Austin L. Gurney ,  Colin K. Watanabe ,  William I. Wood

    IPC分类号： C12N500

    CPC分类号： C07K14/715

    摘要： The present invention is directed to novel cytokine receptors having sequence similarity to AF18497_1 and to nucleic acid molecules encoding those cytokine receptors. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention.

    摘要翻译： 本发明涉及与AF18497_1具有序列相似性的新型细胞因子受体以及编码这些细胞因子受体的核酸分子。 本文还提供了包含那些核酸序列的载体和宿主细胞，包含与异源多肽序列融合的本发明的多肽的嵌合多肽分子，与本发明的多肽结合的抗体以及本发明的多肽的制备方法 发明。

    Materials and methods relating to the transfer of nucleic acid into quiescent cells
    8.
    发明授权
    Materials and methods relating to the transfer of nucleic acid into quiescent cells 失效
    与将核酸转移到静止细胞有关的材料和方法

    公开(公告)号：US06723561B2

    公开(公告)日：2004-04-20

    申请号：US09043665

    申请日：1998-10-05

    申请人： Stephen James Russell ,  Adele Kay Fielding ,  Colin Maurice Casimir

    发明人： Stephen James Russell ,  Adele Kay Fielding ,  Colin Maurice Casimir

    IPC分类号： C12N500

    CPC分类号： C07K14/475 ,  A61K38/00 ,  A61K48/00 ,  C12N2799/027

    摘要： Materials and methods for transferring nucleic acid encoding a polypeptide for treating a disease or disorder into populations of quiescent cells such as haematopoietic stem cells (HSCs), using retroviral packaging cell lines and retroviral particles expressing and display a growth factor such as stem cell factor (SCF) on the cell surface or as a fusion with a viral envelope protein. The present invention also relates to compositions comprising the retroviral packaging cell lines and retroviral particles, and their use in methods of medical treatment, in vivo and ex vivo.

    摘要翻译： 用于将编码用于治疗疾病或病症的多肽的核酸转移至静止细胞群体（例如造血干细胞（HSC））的材料和方法，使用逆转录病毒包装细胞系和表达和显示生长因子如干细胞因子的逆转录病毒颗粒 SCF）或与病毒包膜蛋白的融合物。 本发明还涉及包含逆转录病毒包装细胞系和逆转录病毒颗粒的组合物，以及它们在体内和离体药物治疗方法中的用途。

    Led array for illuminating cell well plates and automated rack system for handling the same
    9.
    发明授权
    Led array for illuminating cell well plates and automated rack system for handling the same 失效
    用于照明电池井的LED阵列和用于处理相同的自动化机架系统

    公开(公告)号：US06680200B2

    公开(公告)日：2004-01-20

    申请号：US10080918

    申请日：2002-02-22

    申请人： Keith Everett

    发明人： Keith Everett

    IPC分类号： C12N500

    CPC分类号： A01G7/04 ,  A01G31/06 ,  B01L9/523 ,  B01L2300/0829 ,  C12M21/02 ,  C12M23/12 ,  C12M23/48 ,  C12M31/10 ,  Y02P60/216 ,  Y10S47/08

    摘要： An assembly for promoting the growth of plant tissues that includes a plurality of plates each defining an array of wells wherein each of the wells contains a tissue sample. Support for the plates is provided by a rack having a plurality of vertically stacked shelves that may include one or more register depressions that urge the plates into predetermined positions. Light for the tissue samples is provided by a plurality of light-emitting diode arrays each mounted on a circuit board. Each circuit board is supported by a respective card edge connector of the rack so that the light-emitting diodes are in proximity to the plates supported on one of the shelves therebelow. Preferably, the light-emitting diode array corresponds to the well array supported in the registered position on the shelf therebelow so that each light-emitting diode is centered above a respective one of the wells.

    摘要翻译： 一种用于促进植物组织生长的组件，其包括多个板，每个板限定孔阵列，其中每个孔包含组织样品。 板的支撑由具有多个垂直堆叠的搁板的支架提供，该搁架可包括一个或多个将板推动到预定位置的寄存器凹陷。 用于组织样品的光由安装在电路板上的多个发光二极管阵列提供。 每个电路板由机架的相应卡片边缘连接器支撑，使得发光二极管靠近支撑在其下的一个搁板上的板。 优选地，发光二极管阵列对应于支撑在其上的搁架上的注册位置中的阱阵列，使得每个发光二极管在相应的一个孔之上居中。