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25 条记录 (耗时 0.039 秒)

    System for monitoring the location of transgenes
    3.
    发明授权
    System for monitoring the location of transgenes 有权
    用于监测转基因位置的系统

    公开(公告)号：US07645865B2

    公开(公告)日：2010-01-12

    申请号：US10428868

    申请日：2003-05-01

    申请人： Stephen James Russell ,  John Morris

    发明人： Stephen James Russell ,  John Morris

    IPC分类号： C07H21/02

    CPC分类号： C07K14/705

    摘要： A novel strategy for monitoring the location of a transgene in a mammal is disclosed. A sodium iodide symporter is genetically fused to either the N-terminus or C-terminus of the product of a transgene through a linker peptide which bears the recognition sequence of a host cell protease. Expression of the transgene confers the activity of the sodium iodide symporter (NIS)to a host cell which expresses the transgene. Subsequent administration of labeled iodine results in transport of the labeled iodine into the cell bearing the NIS, which can then be localized and measured using standard imaging techniques. The system is particularly useful for monitoring the location of therapeutic transgenes and tissue-specific distribution of the therapeutic gene product.

    摘要翻译： 公开了一种监测哺乳动物转基因位置的新颖策略。 碘化钠同向转运体通过承载宿主细胞蛋白酶识别序列的接头肽与转基因产物的N末端或C末端融合。 转基因的表达赋予碘化钠对映体（NIS）对表达转基因的宿主细胞的活性。 随后给予标记的碘导致标记的碘转运到携带NIS的细胞中，然后可以使用标准成像技术进行定位和测量。 该系统特别可用于监测治疗转基因的位置和治疗性基因产物的组织特异性分布。

    Therapeutic methods and compositions using viruses of the recombinant paramyxoviridae family
    7.
    发明授权
    Therapeutic methods and compositions using viruses of the recombinant paramyxoviridae family 有权
    使用重组副粘病毒科的病毒的治疗方法和组合物

    公开(公告)号：US06896881B1

    公开(公告)日：2005-05-24

    申请号：US09667947

    申请日：2000-09-22

    申请人： Stephen James Russell ,  Kah-Whye Peng

    发明人： Stephen James Russell ,  Kah-Whye Peng

    IPC分类号： C07K14/485 ,  C07K14/54 ,  C07K14/65 ,  C07K16/28 ,  C07K16/30 ,  A01N63/00 ,  A61K39/00 ,  A61K39/165 ,  C12N15/00 ,  C12Q1/68

    CPC分类号： C07K16/2896 ,  A61K2039/505 ,  C07K14/485 ,  C07K14/5434 ,  C07K14/65 ,  C07K16/3007 ,  C07K2317/622 ,  C07K2319/00

    摘要： The invention relates to compositions and methods for treating a patient having a tumor in order to reduce tumor size, comprising administering to the patient a replication-competent Paramyxoviridae virus comprising two or more of a) a nucleic acid sequence encoding a heterologous polypeptide, wherein upon administration the heterologous polypeptide is detectable in a biological fluid of the patient, and detection of the heterologous polypeptide is indicative of Paramyxoviridae virus growth in the patient and reduction in tumor size; b) a recombinant F protein, H protein, or M protein of Paramyxoviridae virus that increases fusogenicity of virus with cells; c) a nucleic acid sequence encoding a cytokine; and d) a Paramyxoviridae virus that is specific for cells of the tumor.

    摘要翻译： 本发明涉及用于治疗具有肿瘤的患者以减少肿瘤大小的组合物和方法，包括向患者施用包含两个或更多个a）编码异源多肽的核酸序列的可复制的副粘病毒科病毒，其中在 施用异源多肽在患者的生物流体中是可检测的，并且异源多肽的检测指示患者中副粘病毒病毒生长并减少肿瘤大小; b）副细菌病毒的重组F蛋白，H蛋白或M蛋白，其增加病毒与细胞的融合性; c）编码细胞因子的核酸序列; 和d）对于肿瘤细胞特异的副粘病毒科病毒。

    Gene delivery device and gene delivery method
    8.
    发明授权
    Gene delivery device and gene delivery method 失效
    基因递送装置和基因传递方法

    公开(公告)号：US06821264B1

    公开(公告)日：2004-11-23

    申请号：US09723121

    申请日：2000-11-27

    申请人： Gautam Khurana ,  Stephen James Russell ,  Zvonimir S. Katusic

    发明人： Gautam Khurana ,  Stephen James Russell ,  Zvonimir S. Katusic

    IPC分类号： A61M3700

    CPC分类号： A61K48/0075

    摘要： A gene delivery device for localizing and enhancing the efficacy of gene transfer that provides a contact surface for contacting with a tissue site. By applying a pharmaceutical composition comprising a nucleic acid to a contact surface and contacting the contact surface to a tissue site, a greater than 10-fold increase in transduction efficiency is achieved. In one embodiment of the invention, the device comprises a housing in communication with a contact surface having multiple contact elements, and the pharmaceutical composition is applied to the contact elements through a lumen in the device.

    摘要翻译： 用于定位和增强基因转移功能的基因递送装置，其提供与组织部位接触的接触表面。 通过将包含核酸的药物组合物应用于接触表面并使接触表面与组织部位接触，实现了转导效率提高了10倍以上。 在本发明的一个实施例中，该装置包括与具有多个接触元件的接触表面连通的壳体，并且该药物组合物通过装置中的内腔施加到接触元件。

    System for monitoring the expression of transgenes
    10.
    发明授权
    System for monitoring the expression of transgenes 有权
    用于监测转基因表达的系统

    公开(公告)号：US06632800B1

    公开(公告)日：2003-10-14

    申请号：US09639667

    申请日：2000-08-16

    申请人： Stephen James Russell ,  Kah Whye Peng

    发明人： Stephen James Russell ,  Kah Whye Peng

    IPC分类号： A61K3170

    CPC分类号： C07K14/705

    摘要： A novel strategy for monitoring the expression of a transgene in a patient is disclosed. A marker peptide is genetically fused to either the N-terminus or C-terminus of the product of a transgene through a linker peptide which bears the recognition sequence of a host cell protease. Expression of the transgene results in release of the marker peptide into extracellular body fluid of the patient in proportion to the amount of transgene product. The level of the released marker peptide serves as an indicator of the level of transgene expression. The system is particularly useful for monitoring the expression of therapeutic transgenes and production of the therapeutic gene product.

    摘要翻译： 公开了一种用于监测患者中转基因表达的新颖策略。 标记肽通过携带宿主细胞蛋白酶识别序列的接头肽与转基因产物的N末端或C末端融合。 转基因的表达导致标记肽与转基因产物的量成比例地释放到患者的细胞外体液中。 释放的标记肽的水平作为转基因表达水平的指标。 该系统对于监测治疗转基因的表达和治疗性基因产物的产生特别有用