公开(公告)号：US06589728B2

公开(公告)日：2003-07-08

申请号：US09773824

申请日：2001-01-31

申请人： Marie Csete ,  John Doyle ,  Barbara Wold

发明人： Marie Csete ,  John Doyle ,  Barbara Wold

IPC分类号： C12Q100

CPC分类号： C12N5/0658 ,  A61K35/12 ,  A61K38/1816 ,  A61K48/00 ,  C12N5/0606 ,  C12N5/0619 ,  C12N5/0623 ,  C12N2500/02 ,  C12N2501/115 ,  C12N2501/14 ,  C12N2503/02

摘要： The present invention provides a method of isolating, maintaining, and/or enriching for stem or progenitor cells derived from diverse organ or tissue sources. The invention specifically teaches that these can be accomplished by the controlled use of subatmospheric oxygen culture, and that the precise oxygen level or levels must be determined empirically and/or by reference to physiologic levels within intact functioning organ or tissue.

摘要翻译： 本发明提供了分离，维持和/或富集衍生自不同器官或组织来源的干细胞或祖细胞的方法。 本发明具体地指出，这些可以通过受控使用低于大气压的氧气培养来实现，并且精确的氧水平或水平必须经验地和/或通过参考完整功能器官或组织内的生理水平来确定。

公开(公告)号：US06184035B2

公开(公告)日：2001-02-06

申请号：US09195569

申请日：1998-11-18

申请人： Marie Csete ,  John Doyle ,  Barbara Wold

发明人： Marie Csete ,  John Doyle ,  Barbara Wold

IPC分类号： C12N500

CPC分类号： C12N5/0658 ,  A61K35/12 ,  A61K38/1816 ,  A61K48/00 ,  C12N5/0606 ,  C12N5/0619 ,  C12N5/0623 ,  C12N2500/02 ,  C12N2501/115 ,  C12N2501/14 ,  C12N2503/02

摘要： The present invention provides a method of isolating, maintaining, and/or enriching for stem or progenitor cells derived from diverse organ or tissue sources. The invention specifically teaches that these can be accomplished by the controlled use of subatmospheric oxygen culture, and that the precise oxygen level or levels must be determined empirically and/or by reference to physiologic levels within intact functioning organ or tissue. In particular, culturing skeletal muscle progenitor cells in less than 12% oxygen conditions or under 1% oxygen level.

摘要翻译： 本发明提供了分离，维持和/或富集衍生自不同器官或组织来源的干细胞或祖细胞的方法。 本发明具体地指出，这些可以通过受控使用低于大气压的氧气培养来实现，并且精确的氧水平或水平必须经验地和/或通过参考完整功能器官或组织内的生理水平来确定。 特别地，在小于12％氧气条件或低于1％氧气水平下培养骨骼肌祖细胞。

公开(公告)号：US06759242B1

公开(公告)日：2004-07-06

申请号：US09425624

申请日：1999-10-22

申请人： Marie Csete ,  Sean J. Morrison ,  Barbara Wold ,  David J. Anderson

发明人： Marie Csete ,  Sean J. Morrison ,  Barbara Wold ,  David J. Anderson

IPC分类号： C12N500

CPC分类号： C12N5/0658 ,  A61K35/12 ,  A61K38/1816 ,  A61K48/00 ,  C12N5/0606 ,  C12N5/0619 ,  C12N5/0623 ,  C12N2500/02 ,  C12N2501/115 ,  C12N2501/14 ,  C12N2503/02

摘要： The present invention relates to the growth of cells in culture under conditions that promote differentiation, cell survival, and/or cellular proliferation. More particularly, culturing neural crest stem cells in low oxygen conditions is described.

摘要翻译： 本发明涉及在促进分化，细胞存活和/或细胞增殖的条件下培养细胞的生长。 更具体地，描述了在低氧条件下培养神经嵴干细胞。

公开(公告)号：US06610540B1

公开(公告)日：2003-08-26

申请号：US09425462

申请日：1999-10-22

申请人： Marie Csete ,  John Doyle ,  Barbara J. Wold ,  Ron McKay ,  Lorenz Studer

发明人： Marie Csete ,  John Doyle ,  Barbara J. Wold ,  Ron McKay ,  Lorenz Studer

IPC分类号： C12N500

CPC分类号： C12N5/0658 ,  A61K35/12 ,  A61K38/1816 ,  A61K48/00 ,  C12N5/0606 ,  C12N5/0619 ,  C12N5/0623 ,  C12N2500/02 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/14 ,  C12N2503/02

摘要： The present invention relates to the growth of cells in culture under conditions that promote cell survival, proliferation, and/or cellular differentiation. The present inventors have found that proliferation was promoted and apoptosis reduced when cells were grown in lowered oxygen as compared to environmental oxygen conditions traditionally employed in cell culture techniques. Further, the inventors found that differentiation of precursor cells to specific fates also was enhanced in lowered oxygen where a much greater number and fraction of dopaminergic neurons were obtained when mesencephalic precursors were expanded and differentiated in lowered oxygen conditions. Thus at more physiological oxygen levels the proliferation and differentiation of CNS precursors is enhanced, and lowered oxygen is a useful adjunct for ex vivo generation of specific neuron types. Methods and compositions exploiting these findings are described.

摘要翻译： 本发明涉及在促进细胞存活，增殖和/或细胞分化的条件下培养细胞的生长。 本发明人已经发现，与细胞培养技术中传统使用的环境氧条件相比，当细胞在低氧气中生长时，促进增殖并减少细胞凋亡。 此外，本发明人发现，当降低氧气时，前体细胞分化为特定命运也得到增强，其中当中脑前体在降低的氧条件下扩增和分化时，获得了更多数量和分数的多巴胺能神经元。 因此，在更多的生理氧水平下，CNS前体的增殖和分化被增强，并且降低的氧是用于特定神经元类型的体外产生的有用的辅助剂。 描述了利用这些发现的方法和组合物。