公开(公告)号：US5057418A

公开(公告)日：1991-10-15

申请号：US318255

申请日：1989-03-03

申请人： Takao Uchiyama ,  Kuniji Tanaka ,  Mishio Kawamura

发明人： Takao Uchiyama ,  Kuniji Tanaka ,  Mishio Kawamura

IPC分类号： C12N9/24 ,  C12P19/12 ,  C12P19/14 ,  C12R1/06

CPC分类号： C12Y302/0108 ,  C12N9/88 ,  C12P19/12 ,  C12P19/14 ,  C12R1/06 ,  C12Y302/01007 ,  C12Y402/02018 ,  Y10S435/83

摘要： Disclosed herein is a process for preparing difructose dianhydride III (DFA III) comprising reacting inulin with an inulin lytic enzyme derived from a microorganism belonging to Arthrobacter ilicis. The enzyme employed herein efficiently produces DFA III from inulin and is more stable against heat than conventional enzymes. The present process enables industrial continuous production of DFA III. The preferred strain used herein is Arthrobacter ilicis MCI 2297 (FERM P-9893).

公开(公告)号：US20190002939A1

公开(公告)日：2019-01-03

申请号：US15777932

申请日：2016-04-15

申请人： Jiangnan University

发明人： Wanmeng MU ,  Bo JIANG ,  Shuhuai YU ,  Yingying ZHU ,  Tao ZHANG

IPC分类号： C12P19/12 ,  C12P19/14

CPC分类号： C12P19/12 ,  C12N9/1051 ,  C12N9/88 ,  C12P19/14 ,  C12P19/18 ,  C12Y204/01009 ,  C12Y402/02017 ,  C12Y402/02018

摘要： The invention discloses a high-efficiency synthesis method of difructose anhydride III. The method comprises the following steps: firstly converting sucrose into inulin by using inulosucrase without separating polysaccharide, and then converting inulin by using inulin fructotransferase to synthesize the functional disaccharide difructose anhydride III. The method has the advantages of simple process and high efficiency, and a conversion rate of synthesizing inulin into difructose anhydride III can reach 40%-54%. In order to obtain purer difructose anhydride III, yeast is utilized to remove small molecule monosaccharides in a reaction solution, and the finally obtained purer difructose anhydride III can be easily separated and purified. Thus, the method has broad market application prospects.