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公开(公告)号：US20160312226A1
公开(公告)日：2016-10-27
申请号：US15105925
申请日：2014-12-18
Applicant: Kelvin LEE , Abraham LENHOFF , Kristen VALENTE , Nick LEVY , Yatin GOKARN
Inventor： Kelvin LEE , Abraham LENHOFF , Kristen VALENTE , Nick LEVY , Yatin GOKARN
IPC: C12N15/113 , A61K38/39 , A61K38/17 , A61K38/44 , C12P21/00 , A61K38/48 , A61K38/45 , A61K38/42 , A61K47/26 , C07K16/00 , A61K38/52
CPC classification number: C12N15/1137 , A61K38/1709 , A61K38/1741 , A61K38/1761 , A61K38/1774 , A61K38/39 , A61K38/42 , A61K38/44 , A61K38/443 , A61K38/45 , A61K38/4813 , A61K38/482 , A61K38/488 , A61K38/52 , A61K47/26 , C07K16/00 , C12N9/20 , C12N2310/10 , C12N2310/14 , C12N2310/20 , C12P21/005 , C12P21/02 , C12Y104/03013 , C12Y111/01015 , C12Y205/01018 , C12Y207/04006 , C12Y207/11001 , C12Y301/01034 , C12Y304/16005 , C12Y304/21 , C12Y304/23005 , C12Y502/01008 , C12Y503/04001
Abstract: The invention relates a method for producing a stable recombinant protein, comprising growing a non-naturally occurring host cell in a culture medium to produce a recombinant protein, and making a composition comprising the recombinant protein and a polysorbate. The production of endogenous lipoprotein lipase by the host cell is reduced. The endogenous lipoprotein lipase is present in the composition in a small amount, and is capable of degrading the polysorbate. The invention also relates to the relevant host cells and compositions, and preparation thereof.
Abstract translation: 本发明涉及一种生产稳定重组蛋白的方法，包括在培养基中培养非天然存在的宿主细胞以产生重组蛋白，以及制备包含重组蛋白和聚山梨酯的组合物。 由宿主细胞产生的内源性脂蛋白脂肪酶降低。 内源性脂蛋白脂肪酶以少量存在于组合物中，并且能够降解聚山梨醇酯。 本发明还涉及相关宿主细胞和组合物及其制备。
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2.发明申请IDENTIFICATION OF HUMAN MOUSE DOUBLE MINUTE 2 HOMOLOG NUCLEOTIDE SEQUENCES 有权人类双胞胎分子标记2 HOMOLOG核苷酸序列公开(公告)号：US20130071845A1
公开(公告)日：2013-03-21
申请号：US13680247
申请日：2012-11-19
Applicant: RYOGEN LLC
Inventor： James Ryan
IPC: C12Q1/68
CPC classification number: A61K31/7088 , C12N9/48 , C12Q1/6876 , C12Q1/6883 , C12Y304/16005
Abstract: The invention is directed to isolated genomic polynucleotide fragments that encode human carboxypeptidase M and human mouse double minute 2 homolog, vectors and hosts containing these fragments and fragments hybridizing to noncoding regions as well as antisense oligonucleotides to these fragments. The invention is further directed to methods of using these fragments to obtain human carboxypeptidase M and human mouse double minute 2 homolog and to diagnose, treat, prevent and/or ameliorate a pathological disorder.
Abstract translation: 本发明涉及编码人类羧肽酶M的分离的基因组多核苷酸片段和含有这些片段的人类小鼠双分子2同系物，载体和宿主，并且与非编码区杂交的片段以及与这些片段的反义寡核苷酸杂交。 本发明进一步涉及使用这些片段获得人类羧肽酶M和人类小鼠双分钟2同系物并诊断，治疗，预防和/或改善病理性疾病的方法。
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3.发明授权Isolated genomic polynucleotide fragments from chromosome 12 that encode human mouse double minute 2 homolog 有权来自染色体12的编码人小鼠双分钟2同源物的分离的基因组多核苷酸片段公开(公告)号：US07964357B1
公开(公告)日：2011-06-21
申请号：US12795864
申请日：2010-06-08
Applicant: James W Ryan
Inventor： James W Ryan
CPC classification number: A61K31/7088 , C12N9/48 , C12Q1/6876 , C12Q1/6883 , C12Y304/16005
Abstract: The invention is directed to isolated genomic polynucleotide fragments that encode human carboxypeptidase M and human mouse double minute 2 homolog, vectors and hosts containing these fragments and fragments hybridizing to noncoding regions as well as antisense oligonucleotides to these fragments. The invention is further directed to methods of using these fragments to obtain human carboxypeptidase M and human mouse double minute 2 homolog and to diagnose, treat, prevent and/or ameliorate a pathological disorder.
Abstract translation: 本发明涉及编码人类羧肽酶M的分离的基因组多核苷酸片段和含有这些片段的人类小鼠双分子2同系物，载体和宿主，并且与非编码区杂交的片段以及与这些片段的反义寡核苷酸杂交。 本发明进一步涉及使用这些片段获得人类羧肽酶M和人类小鼠双分钟2同系物并诊断，治疗，预防和/或改善病理性疾病的方法。
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4.发明授权Method for producing proteins suitable for treating lysosomal storage disorders 有权生产适用于治疗溶酶体贮积病的蛋白质的方法公开(公告)号：US07241442B2
公开(公告)日：2007-07-10
申请号：US10733501
申请日：2003-12-11
Applicant: Alessandra D'Azzo , Erik Jacobus Bonten
Inventor： Alessandra D'Azzo , Erik Jacobus Bonten
CPC classification number: C12Y302/01018 , A61K38/00 , C12N9/2402 , C12N9/48 , C12Y304/16005
Abstract: The present invention is based on the discovery that proteins produced in insect cell cultures are glycosylated in a unique manner that causes them to be selectively imported by cells that express mannose receptors on their membranes, particularly macrophages. Proteins that are selectively imported into cells containing mannose receptors are provided, as well as pharmaceutical compositions containing such proteins and methods for producing such proteins. Application of the present invention to produce proteins useful for treating lysosomal storage disorders is also disclosed. Engineering of cells to express mannose receptors so that they will selectively import proteins produced in insect cells is also taught, as well as a protein targeting system using such cells and proteins. Finally, an improved elution buffer for the purification of proteins produced in insect cells from a Concanavalin A column is provided.
Abstract translation: 本发明基于以下发现：昆虫细胞培养物中产生的蛋白质以独特的方式进行糖基化，使得它们可以通过在其膜，特别是巨噬细胞上表达甘露糖受体的细胞选择性地导入。 提供选择性导入含有甘露糖受体的细胞的蛋白质，以及含有这些蛋白质的药物组合物和用于生产这种蛋白质的方法。 还公开了本发明用于产生可用于治疗溶酶体储存障碍的蛋白质的应用。 表达甘露糖受体的细胞的工程也被教导，以及它们将选择性地导入在昆虫细胞中产生的蛋白质，以及使用这种细胞和蛋白质的蛋白质靶向系统。 最后，提供了一种用于纯化从刀豆球蛋白A柱的昆虫细胞中产生的蛋白质的改进的洗脱缓冲液。
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公开(公告)号：US20060286597A1
公开(公告)日：2006-12-21
申请号：US11507811
申请日：2006-08-21
Applicant: Mu-En Lee , Matthew Layne , Shaw-Fang Yet
Inventor： Mu-En Lee , Matthew Layne , Shaw-Fang Yet
CPC classification number: C12N9/48 , A01K2217/05 , A01K2217/075 , A61K38/00 , C07K14/47 , C12N9/6421 , C12Y304/16005 , G01N33/6893 , G01N2333/948 , G01N2800/062
Abstract: The invention features a aortic carboxypeptidase-like polypeptide (ACLP), DNA encoding ACLP, and methods of detecting genetic alterations associated with abdominal wall defects.
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Patent Agency Ranking
公开(公告)号：US07094878B2
公开(公告)日：2006-08-22
申请号：US10238876
申请日：2002-09-10
Applicant: Mu-En Lee , Matthew D. Layne , Shaw-Fang Yet
Inventor： Mu-En Lee , Matthew D. Layne , Shaw-Fang Yet
IPC: C07K14/47
CPC classification number: C12N9/48 , A01K2217/05 , A01K2217/075 , A61K38/00 , C07K14/47 , C12N9/6421 , C12Y304/16005 , G01N33/6893 , G01N2333/948 , G01N2800/062
Abstract: The invention features a aortic carboxypeptidase-like polypeptide (ACLP), DNA encoding ACLP, and methods of detecting genetic alterations associated with abdominal wall defects.
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公开(公告)号：US09138465B2
公开(公告)日：2015-09-22
申请号：US14114122
申请日：2012-04-27
Applicant: Keiichi Ozono , Takanobu Otomo , Norio Sakai
Inventor： Keiichi Ozono , Takanobu Otomo , Norio Sakai
CPC classification number: A61K38/47 , A61K38/48 , A61K38/4813 , A61K38/488 , C12N5/06 , C12Y302/0102 , C12Y302/01021 , C12Y302/01022 , C12Y302/01023 , C12Y302/01024 , C12Y302/01031 , C12Y302/01046 , C12Y302/01051 , C12Y302/01052 , C12Y304/14001 , C12Y304/16005 , C12Y304/22001 , C12Y304/22038 , C12Y304/23005 , C12Y304/23034 , A61K2300/00
Abstract: The purpose of the present invention is to provide, in a simple and also inexpensive manner, a pharmaceutical composition which comprises a plurality of lysosomal enzymes and is effective in treating lysosomal storage disease caused by a deficiency in a plurality of lysosomal enzymes. Provided is a pharmaceutical composition for treating lysosomal storage disease, the composition comprising as an active ingredient a lysosomal enzyme group obtained from cells derived from a subject who does not suffer from lysosomal storage disease.
Abstract translation: 本发明的目的是以简单且便宜的方式提供包含多种溶酶体酶的药物组合物，并且有效地治疗由多种溶酶体酶缺乏引起的溶酶体贮积病。 本发明提供一种用于治疗溶酶体贮积病的药物组合物，该组合物含有从不患有溶酶体贮积病的受试者的细胞获得的溶酶体酶组分作为活性成分。
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8.发明授权公开(公告)号：US08956819B2
公开(公告)日：2015-02-17
申请号：US13680247
申请日：2012-11-19
Applicant: Ryogen LLC
Inventor： James Ryan
IPC: C12N9/48 , C07H21/04 , C12Q1/68 , A61K31/7088
CPC classification number: A61K31/7088 , C12N9/48 , C12Q1/6876 , C12Q1/6883 , C12Y304/16005
Abstract: The invention is directed to isolated genomic polynucleotide fragments that encode human carboxypeptidase M and human mouse double minute 2 homolog, vectors and hosts containing these fragments and fragments hybridizing to noncoding regions as well as antisense oligonucleotides to these fragments. The invention is further directed to methods of using these fragments to obtain human carboxypeptidase M and human mouse double minute 2 homolog and to diagnose, treat, prevent and/or ameliorate a pathological disorder.
Abstract translation: 本发明涉及编码人类羧肽酶M的分离的基因组多核苷酸片段和含有这些片段的人类小鼠双分子2同系物，载体和宿主，并且与非编码区杂交的片段以及与这些片段的反义寡核苷酸杂交。 本发明进一步涉及使用这些片段获得人类羧肽酶M和人类小鼠双分钟2同系物并诊断，治疗，预防和/或改善病理性疾病的方法。
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公开(公告)号：US20150030723A1
公开(公告)日：2015-01-29
申请号：US14512713
申请日：2014-10-13
Applicant: Novozymes A/S , Chr. Hansen A/S
Inventor： Jeppe Wegener Tams , Preben Nielsen
CPC classification number: A23C9/1209 , A23C9/1216 , A23C9/1275 , A23Y2220/00 , C12N9/485 , C12N9/80 , C12Y304/16005 , C12Y305/01
Abstract: The present invention relates to a method for producing an acidified milk drink using an enzyme which reduces the isoelectric point of the milk proteins. The invention also relates to a novel enzyme having deamidase activity and its use in production of an acidified milk drink.
Abstract translation: 本发明涉及使用降低乳蛋白质等电点的酶来生产酸化乳饮料的方法。 本发明还涉及具有脱酰胺酶活性的新型酶及其在酸化乳饮料生产中的应用。
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10.发明授权Isolated genomic polynucleotide fragments from chromosome 12 that encode human mouse double minute 2 homolog 有权来自染色体12的编码人小鼠双分钟2同源物的分离的基因组多核苷酸片段公开(公告)号：US08313910B1
公开(公告)日：2012-11-20
申请号：US13116140
申请日：2011-05-26
Applicant: James W Ryan
Inventor： James W Ryan
CPC classification number: A61K31/7088 , C12N9/48 , C12Q1/6876 , C12Q1/6883 , C12Y304/16005
Abstract: The invention is directed to isolated genomic polynucleotide fragments that encode human carboxypeptidase M and human mouse double minute 2 homolog, vectors and hosts containing these fragments and fragments hybridizing to noncoding regions as well as antisense oligonucleotides to these fragments. The invention is further directed to methods of using these fragments to obtain human carboxypeptidase M and human mouse double minute 2 homolog and to diagnose, treat, prevent and/or ameliorate a pathological disorder.
Abstract translation: 本发明涉及编码人类羧肽酶M的分离的基因组多核苷酸片段和含有这些片段的人类小鼠双分子2同系物，载体和宿主，并且与非编码区杂交的片段以及与这些片段的反义寡核苷酸杂交。 本发明进一步涉及使用这些片段获得人类羧肽酶M和人类小鼠双分钟2同系物并诊断，治疗，预防和/或改善病理性疾病的方法。
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