公开(公告)号：US20230159969A1

公开(公告)日：2023-05-25

申请号：US17995367

申请日：2021-04-02

Applicant: RENSSELAER POLYTECHNIC INSTITUTE ,  OTSUKA PHARMACEUTICAL FACTORY, INC. ,  KIRIN BIOMATERIALS CO., LTD.

Inventor： Kenta FUKUNAGA ,  Aki NISHIBARA ,  Ryosuke KATO ,  Masahiro KURATSU ,  Mikiro HAYASHI ,  Shin-ichi HASHIMOTO

IPC: C12P19/26 ,  C12P19/04 ,  C12N9/12

CPC classification number: C12P19/26 ,  C12P19/04 ,  C12N9/1241 ,  C12Y207/07004 ,  C12N9/1205 ,  C12Y207/01025

Abstract: The invention provides a method for producing sulfated polysaccharides by reacting a PAPS production/regeneration system utilizing the metabolic activity of a microorganism or a treated matter thereof with a microorganism expressing a sulfation enzyme or a treated matter or extract thereof upon mixing of inexpensive raw materials such as magnesium sulfate. The invention also provides a method for producing PAPS from inexpensive raw materials. The methods involve preparing a transformant (a) of a bacterium of the genus Corynebacterium, which contains a gene encoding an ATP sulfurylase and a gene encoding an APS kinase, which are expressible, and in which a cell plasma membrane of the transformant (a) is substance-permeable, or a treated matter of the transformant (a), and conducting a reaction for producing PAPS by using a reaction solution containing ATP or an ATP source, a sulfate ion source, and the transformant (a) or the treated matter thereof.

公开(公告)号：US20180073033A1

公开(公告)日：2018-03-15

申请号：US15693710

申请日：2017-09-01

Applicant: Wisconsin Alumni Research Foundation

Inventor： Maria Sardi ,  Audrey P. Gasch

IPC: C12N15/81 ,  C12N9/12 ,  C07K14/395 ,  C12N9/04 ,  C12P7/10

CPC classification number: C12N15/81 ,  C07K14/395 ,  C12N9/0006 ,  C12N9/1205 ,  C12N15/52 ,  C12P7/10 ,  C12P7/649 ,  C12Y101/0117 ,  C12Y207/01025 ,  Y02E50/13 ,  Y02E50/16 ,  Y02E50/343

Abstract: The present invention provides isolated gene sequences useful in increasing lignocellulosic toxin tolerance in yeast. Such engineered yeast are useful in methods of biofuel production, particularly ethanol production. Methods of bioengineering recombinant yeast with increased lignocellulosic toxin tolerance are also provided.

公开(公告)号：US20170183693A1

公开(公告)日：2017-06-29

申请号：US15502328

申请日：2015-08-21

Applicant: Danmarks Tekniske Universitet

Inventor： Christian Bille Jendresen ,  Alex Toftgaard Nielsen

IPC: C12P7/14 ,  C12P7/10 ,  C12N15/52 ,  C12N15/81

CPC classification number: C12P7/14 ,  C12N9/13 ,  C12N15/52 ,  C12N15/81 ,  C12P5/005 ,  C12P7/10 ,  C12P2201/00 ,  C12P2203/00 ,  C12Y207/01025 ,  C12Y207/07004 ,  C12Y208/02001 ,  C12Y301/03004 ,  Y02E50/16

Abstract: The present invention relates to the production of hydrolyzates from a lignocellulose-containing material, and to fermentation of the hydrolyzates. More specifically, the present invention relates to the detoxification of phenolic inhibitors and toxins formed during the processing of lignocellulose-containing material by enzymatically sulfating the phenolic inhibitors and toxins using aryl sulfotranseferases.