公开(公告)号：US20230159969A1

公开(公告)日：2023-05-25

申请号：US17995367

申请日：2021-04-02

Applicant: RENSSELAER POLYTECHNIC INSTITUTE ,  OTSUKA PHARMACEUTICAL FACTORY, INC. ,  KIRIN BIOMATERIALS CO., LTD.

Inventor： Kenta FUKUNAGA ,  Aki NISHIBARA ,  Ryosuke KATO ,  Masahiro KURATSU ,  Mikiro HAYASHI ,  Shin-ichi HASHIMOTO

IPC: C12P19/26 ,  C12P19/04 ,  C12N9/12

CPC classification number: C12P19/26 ,  C12P19/04 ,  C12N9/1241 ,  C12Y207/07004 ,  C12N9/1205 ,  C12Y207/01025

Abstract: The invention provides a method for producing sulfated polysaccharides by reacting a PAPS production/regeneration system utilizing the metabolic activity of a microorganism or a treated matter thereof with a microorganism expressing a sulfation enzyme or a treated matter or extract thereof upon mixing of inexpensive raw materials such as magnesium sulfate. The invention also provides a method for producing PAPS from inexpensive raw materials. The methods involve preparing a transformant (a) of a bacterium of the genus Corynebacterium, which contains a gene encoding an ATP sulfurylase and a gene encoding an APS kinase, which are expressible, and in which a cell plasma membrane of the transformant (a) is substance-permeable, or a treated matter of the transformant (a), and conducting a reaction for producing PAPS by using a reaction solution containing ATP or an ATP source, a sulfate ion source, and the transformant (a) or the treated matter thereof.

公开(公告)号：US20230175028A1

公开(公告)日：2023-06-08

申请号：US17995352

申请日：2021-04-02

Applicant: RENSSELAER POLYTECHNIC INSTITUTE ,  OTSUKA PHARMACEUTICAL FACTORY, INC. ,  KIRIN BIOMATERIALS CO., LTD.

Inventor： Ayano KOMINE ,  Mikiro HAYASHI

IPC: C12P19/26 ,  C12N15/70 ,  C12N1/20

CPC classification number: C12P19/26 ,  C12N1/205 ,  C12N15/70

Abstract: An object of the present invention is to provide a method for efficiently producing heparosan by improving a heparosan-producing ability through genetic modification of a bacterium of the genus Escherichia which has a heparosan-producing ability. The present invention relates to a bacterium of the genus Escherichia which has a genetic modification that increases an expression of a kpsS gene, and which has a heparosan-producing ability; and a method for producing heparosan using the bacterium.