公开(公告)号：US20150007363A1

公开(公告)日：2015-01-01

申请号：US14321467

申请日：2014-07-01

申请人： UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.

发明人： L. CURTIS HANNAH ,  THOMAS W. GREENE

IPC分类号： C12N15/82 ,  C12Q1/48 ,  C12N9/12

CPC分类号： C12N15/8245 ,  C12N9/1051 ,  C12N9/1241 ,  C12N15/8271 ,  C12Q1/485 ,  C12Y204/01021 ,  C12Y207/07027

摘要： The subject invention pertains to novel mutant polynucleotide molecules that encode enzymes that have increased heat stability. These polynucleotides, when expressed in plants, result in increased yield in plants grown under conditions of heat stress. The polynucleotide molecules of the subject invention encode maize endosperm ADP glucose pyrophosphorylase (AGP) and soluble starch synthase (SSS) enzyme activities. Plants and plant tissue bred to contain, or transformed with, the mutant polynucleotides, and expressing the polypeptides encoded by the polynucleotides, are also contemplated by the present invention. The subject invention also concerns methods for isolating polynucleotides and polypeptides contemplated within the scope of the invention. Methods for increasing yield in plants grown under conditions of heat stress are also provided.

摘要翻译： 本发明涉及编码具有增加的热稳定性的酶的新型突变体多核苷酸分子。 当在植物中表达时，这些多核苷酸导致在热胁迫条件下生长的植物中的产量增加。 本发明的多核苷酸分子编码玉米胚乳ADP葡萄糖焦磷酸化酶（AGP）和可溶性淀粉合成酶（SSS）酶活性。 繁殖含有突变多核苷酸或转化了突变体多核苷酸并表达多核苷酸编码的多肽的植物和植物组织也被本发明考虑。 本发明还涉及分离本发明范围内考虑的多核苷酸和多肽的方法。 还提供了在热胁迫条件下生长的植物中提高产量的方法。

公开(公告)号：US20130344553A1

公开(公告)日：2013-12-26

申请号：US13997242

申请日：2011-12-20

申请人： James Weifu Lee

发明人： James Weifu Lee

IPC分类号： C12P7/16

CPC分类号： C12P7/16 ,  C12N15/1137 ,  C12N2310/11 ,  C12N2310/14 ,  C12P7/04 ,  C12Y204/01021 ,  Y02E50/10

摘要： Designer Calvin-cycle-channeled and hydrogenotrophic biofuel-production pathways, the associated designer genes and designer transgenic organisms for autotrophic production of butanol and related higher alcohols from carbon dioxide, hydrogen, and/or water are provided. The butanol and related higher alcohols include 1-butanol, 2-methyl-1-butanol, isobutanol, 3-methyl-1-butanol, 1-hexanol, 1-octanol, 1-pentanol, 1-heptanol, 3-methyl-1-pentanol, 4-methyl-1-hexanol, 5-methyl-1-heptanol, 4-methyl-1-pentanol, 5-methyl-1-hexanol, and 6-methyl-1-heptanol. The designer autotrophic organisms such as designer transgenic oxyphotobacteria and algae comprise designer Calvin-cycle-channeled and hydrogenotrophic pathway gene(s) and biosafety-guarding technology for enhanced autotrophic production of butanol and related higher alcohols from carbon dioxide and water.

摘要翻译： 提供了设计者卡尔文循环通道和氢营养生物燃料生产途径，相关的设计者基因和设计者转基因生物用于从二氧化碳，氢气和/或水自动生产丁醇和相关的高级醇。 丁醇和相关的高级醇包括1-丁醇，2-甲基-1-丁醇，异丁醇，3-甲基-1-丁醇，1-己醇，1-辛醇，1-戊醇，1-庚醇，3-甲基-1- 戊醇，4-甲基-1-己醇，5-甲基-1-庚醇，4-甲基-1-戊醇，5-甲基-1-己醇和6-甲基-1-庚醇。 设计者自养生物如设计者转基因生物光合细菌和藻类包括设计者卡尔文循环通道和氢营养途径基因和生物安全保护技术，用于增强二氧化碳和水中丁醇和相关高级醇的自养生产。

公开(公告)号：US06784338B1

公开(公告)日：2004-08-31

申请号：US08070455

申请日：1993-11-24

申请人： Per Hofvander ,  Per T. Persson ,  Anneli Tallberg ,  Olle Wikström

发明人： Per Hofvander ,  Per T. Persson ,  Anneli Tallberg ,  Olle Wikström

IPC分类号： C12N1582

CPC分类号： C12N15/1137 ,  C12N9/1051 ,  C12N15/8245 ,  C12P19/04 ,  C12Y204/01021

摘要： Genetically engineered modification of potato for suppressing the formation of amylose-type starch is described. Three fragments for insertion in the antisense direction into the potato genome are also described. Moreover, antisense constructs, genes and vectors comprising said antisense fragments are described. Further a promoter for the gene coding for formation of granule-bound starch synthase and also the gene itself are described. Also cells, plants, tubers, microtubers and seeds of potato comprising said antisense fragments are described. Finally, amylopectin-type starch, both native and derivatised, derived from the potato that is modified in a genetically engineered manner, as well as a method of suppressing amylose formation in potato are described.

摘要翻译： 描述了用于抑制直链淀粉型淀粉形成的马铃薯的遗传工程改造。还描述了用于在反义方向插入马铃薯基因组中的三个片段。 此外，描述了包含所述反义片段的反义构建体，基因和载体。 还描述了编码形成颗粒结合淀粉合成酶的基因的启动子以及基因本身。还描述了包含所述反义片段的马铃薯的细胞，植物，块茎，微块茎和种子。最后，支链淀粉型淀粉 描述了从基因工程改造的马铃薯衍生的天然和衍生的，以及抑制马铃薯中直链淀粉形成的方法。

公开(公告)号：US5824798A

公开(公告)日：1998-10-20

申请号：US470720

申请日：1995-06-06

申请人： Anneli Tallberg ,  Per Hofvander ,  Per T. Persson ,  Olle Wikstrom

发明人： Anneli Tallberg ,  Per Hofvander ,  Per T. Persson ,  Olle Wikstrom

IPC分类号： A01H5/00 ,  C08B30/20 ,  C08B35/04 ,  C08B35/08 ,  C12N5/10 ,  C12N9/10 ,  C12N9/42 ,  C12N15/09 ,  C12N15/113 ,  C12N15/29 ,  C12N15/56 ,  C12N15/82 ,  C12P19/04 ,  C07H1/06 ,  C07H1/08

CPC分类号： C12N15/1137 ,  C12N15/8245 ,  C12N9/1051 ,  C12P19/04 ,  C12Y204/01021

摘要： Genetically engineered modification of potato for suppressing formation of amylose-type starch is described. Three fragments for insertion in the antisense direction into the potato genome are also described. Moreover, antisense constructs, genes and vectors comprising said antisense fragments are described. Further a promoter for the gene coding for formation of granule-bound starch synthase and also the gene itself are described. Also cells, plants, tubers, microtubers and seeds of potato comprising said antisense construct are described. Finally, amylopectin-type starch, both native and derivatized, derived from the potato that is modified in a genetically engineered manner, as well as a method of suppressing amylose formation in potato are described.

摘要翻译： 描述了用于抑制直链淀粉型淀粉形成的马铃薯的遗传工程改造。 还描述了用于在反义方向插入到马铃薯基因组中的三个片段。 此外，描述了包含所述反义片段的反义构建体，基因和载体。 另外还描述了编码结合淀粉合成酶的基因的启动子以及基因本身。 还描述了包含所述反义构建体的马铃薯的细胞，植物，块茎，微块茎和种子。 最后，描述了从基因工程改造的马铃薯衍生的天然和衍生的支链淀粉型淀粉，以及抑制马铃薯中直链淀粉形成的方法。

公开(公告)号：US20240218400A1

公开(公告)日：2024-07-04

申请号：US18555290

申请日：2022-04-15

申请人： POET RESEARCH, INC.

发明人： Cory J. Sarks ,  Alex T. Mccurdy ,  Andrew Manning ,  Steven T. Bly

IPC分类号： C12P7/06 ,  C12N9/10 ,  C12P7/6436

CPC分类号： C12P7/06 ,  C12N9/1051 ,  C12P7/6436 ,  C12Y204/01021

摘要： Methods of enhancing corn oil recovery from a bio-refinery using recycled by-products of the process including emulsifiers in the form of emulsions, defatted emulsions, precipitates thereof, and/or dried products thereof.

公开(公告)号：US20030135883A1

公开(公告)日：2003-07-17

申请号：US10044543

申请日：2002-01-11

发明人： George W. Singletary ,  Lan Zhou

IPC分类号： A01H001/00 ,  C12N015/82 ,  C07H021/04 ,  C12N005/04

CPC分类号： C12N9/1051 ,  C12N15/8245 ,  C12Y204/01021

摘要： The invention provides isolated nucleic acids and their encoded proteins that are involved in starch biosynthesis. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions. The present invention provides methods and compositions relating to altering the amount and/or morphology of starch in plants.

摘要翻译： 本发明提供了参与淀粉生物合成的分离的核酸及其编码的蛋白质。 本发明还提供了重组表达盒，宿主细胞，转基因植物和抗体组合物。 本发明提供了关于改变植物中淀粉的量和/或形态的方法和组合物。

公开(公告)号：US20030087369A1

公开(公告)日：2003-05-08

申请号：US10138075

申请日：2002-05-02

发明人： Karen E. Broglie ,  Karlene H. Butler ,  Leslie T. Harvell ,  Jonathan E. Lighter ,  Emil M. Orozco JR.

IPC分类号： C12P021/02 ,  C07H021/04 ,  C12N009/26 ,  C12N005/04

CPC分类号： C12N15/8245 ,  C12N9/1051 ,  C12Y204/01021

摘要： This invention relates to isolated nucleic acid fragments encoding granule-bound starch synthase. The invention also relates to the construction of a recombinant DNA construct encoding all or a portion of the granule-bound starch synthase, in sense or antisense orientation, wherein expression of the recombinant DNA construct results in production of altered levels of the granule-bound starch synthase in a transformed host cell.

摘要翻译： 本发明涉及编码颗粒结合淀粉合成酶的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码全部或一部分颗粒结合的淀粉合成酶的重组DNA构建体，其中重组DNA构建体的表达导致产生改变的颗粒结合淀粉水平 合成酶在转化的宿主细胞中。

公开(公告)号：US20020194642A1

公开(公告)日：2002-12-19

申请号：US10079478

申请日：2002-02-19

发明人： L. Curtis Hannah ,  Thomas W. Greene

IPC分类号： A01H005/00 ,  C07H021/04 ,  C12N009/44 ,  C12P021/02 ,  C12N005/04

CPC分类号： C12N9/1241 ,  C12N9/1051 ,  C12N15/8245 ,  C12N15/8271 ,  C12Y204/01021 ,  C12Y207/07027

摘要： The subject invention pertains to novel mutant polynucleotide molecules that encode enzymes that have increased heat stability. These polynucleotides, when expressed in plants, result in increased yield in plants grown under conditions of heat stress. The polynucleotide molecules of the subject invention encode maize endosperm ADP glucose pyrophosphorylase (AGP) and soluble starch synthase (SSS) enzyme activities. Plants and plant tissue bred to contain, or transformed with, the mutant polynucleotides, and expressing the polypeptides encoded by the polynucleotides, are also contemplated by the present invention. The subject invention also concerns methods for isolating polynucleotides and polypeptides contemplated within the scope of the invention. Methods for increasing yield in plants grown under conditions of heat stress are also provided.

摘要翻译： 本发明涉及编码具有增加的热稳定性的酶的新型突变体多核苷酸分子。 当在植物中表达时，这些多核苷酸导致在热胁迫条件下生长的植物中的产量增加。 本发明的多核苷酸分子编码玉米胚乳ADP葡萄糖焦磷酸化酶（AGP）和可溶性淀粉合成酶（SSS）酶活性。 繁殖含有突变体多核苷酸或转化了突变多核苷酸并表达多核苷酸编码的多肽的植物和植物组织也被本发明考虑。 本发明还涉及分离本发明范围内考虑的多核苷酸和多肽的方法。 还提供了在热胁迫条件下生长的植物中提高产量的方法。

公开(公告)号：US09988641B2

公开(公告)日：2018-06-05

申请号：US15135106

申请日：2016-04-21

申请人： Corn Products Development, Inc.

发明人： Hongxin Jiang ,  Brad Ostrander ,  Chris Lane

IPC分类号： C12N15/00 ,  C12N15/82 ,  A01H5/10 ,  A01H1/02 ,  C12N9/10 ,  A01H1/04 ,  A01G1/00 ,  C08B30/04

CPC分类号： C12N15/8245 ,  A01G22/00 ,  A01H1/02 ,  A01H1/04 ,  A01H5/10 ,  C08B30/044 ,  C12N9/1051 ,  C12N15/8241 ,  C12Y204/01021 ,  C12Y204/01242

摘要： This invention disclosure relates to novel maize starch. The starch can be made from the newly developed waxy sugary-2 double-mutant maize that has low activity of Granule Bound Starch Synthase I (GBSSI), which results in low amylose level. The starch from newly developed waxy sugary-2 double-mutant is freeze-thaw stable and has high viscosity. In comparison with the starch of the existing waxy sugary-2 double-mutant maize, the new waxy sugary-2 double-mutant maize starch showed, inter alia, improved pasting profile, starch granule integrity, larger starch granule size, and higher viscosity.

公开(公告)号：US08986963B2

公开(公告)日：2015-03-24

申请号：US13075153

申请日：2011-03-29

申请人： James Weifu Lee

发明人： James Weifu Lee

IPC分类号： C12P7/16 ,  C12P7/04 ,  C12N15/113 ,  C12Q1/00

CPC分类号： C12P7/16 ,  C12N15/1137 ,  C12N2310/11 ,  C12N2310/14 ,  C12P7/04 ,  C12Q1/00 ,  C12Y204/01021 ,  Y02E50/10

摘要： Designer Calvin-cycle-channeled and photosynthetic NADPH-enhanced pathways, the associated designer genes and designer transgenic photosynthetic organisms for photobiological production of butanol and related higher alcohols from carbon dioxide and water are provided. The butanol and related higher alcohols include 1-butanol, 2-methyl-1-butanol, isobutanol, 3-methyl-1-butanol, 1-hexanol, 1-octanol, 1-pentanol, 1-heptanol, 3-methyl-1-pentanol, 4-methyl-1-hexanol, 5-methyl-1-heptanol, 4-methyl-1-pentanol, 5-methyl-1-hexanol, and 6-methyl-1-heptanol. The designer photosynthetic organisms such as designer transgenic oxyphotobacteria and algae comprise designer Calvin-cycle-channeled and photosynthetic NADPH-enhanced pathway gene(s) and biosafety-guarding technology for enhanced photobiological production of butanol and related higher alcohols from carbon dioxide and water.

摘要翻译： 提供了卡尔文循环通道和光合NADPH增强途径的设计，相关的设计者基因和设计者转基因光合生物用于从二氧化碳和水中光生物生产丁醇和相关的高级醇。 丁醇和相关的高级醇包括1-丁醇，2-甲基-1-丁醇，异丁醇，3-甲基-1-丁醇，1-己醇，1-辛醇，1-戊醇，1-庚醇，3-甲基-1- 戊醇，4-甲基-1-己醇，5-甲基-1-庚醇，4-甲基-1-戊醇，5-甲基-1-己醇和6-甲基-1-庚醇。 设计者光合生物如设计者转基因生物光合细菌和藻类包括设计者卡尔文 - 循环通道和光合NADPH增强的途径基因和用于从二氧化碳和水增强丁醇和相关高级醇的光生物生产的生物安全保护技术。