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1.发明授权公开(公告)号：US06677132B1
公开(公告)日：2004-01-13
申请号：US10009882
申请日：2002-03-28
申请人： Thomas Hofler , Peter Holzhauer , Eckehard Walitza
发明人： Thomas Hofler , Peter Holzhauer , Eckehard Walitza
IPC分类号： C12Q102
CPC分类号： C12M41/36 , C12Q1/04 , C12Q2304/44 , G01N33/1866
摘要： The present invention relates to a device and a method for determining the concentration of organisms in a fluid, where metabolite parameters are recorded by means of a data acquisition unit and subsequently converted.
摘要翻译： 本发明涉及一种用于确定流体中生物体的浓度的装置和方法，其中通过数据获取单元记录代谢物参数并随后转化。
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公开(公告)号：US07338778B2
公开(公告)日：2008-03-04
申请号：US10957802
申请日：2004-10-04
申请人： J. Bruce Pitner , John Jacob Hemperly , Richard D. Guarino , Magdalena Wodnicka , David T. Stitt , Gregory J. Burrell , Timothy G. Foley, Jr. , Patrick Shawn Beaty
发明人： J. Bruce Pitner , John Jacob Hemperly , Richard D. Guarino , Magdalena Wodnicka , David T. Stitt , Gregory J. Burrell , Timothy G. Foley, Jr. , Patrick Shawn Beaty
IPC分类号： C12Q1/04
CPC分类号： C12Q1/18 , C12Q1/04 , C12Q2304/44 , G06F19/00 , Y02A90/26
摘要： The present invention relates to methods for detection and evaluation of metabolic activity of eukaryotic and/or prokaryotic cells based upon their ability to consume dissolved oxygen. The methods utilize a luminescence detection system which makes use of the sensitivity of the luminescent emission of certain compounds to the presence of oxygen, which quenches (diminishes) the compound's luminescent emission in a concentration dependent manner. Respiring eukaryotic and/or prokaryotic cells will affect the oxygen concentration of a liquid medium in which they are immersed. Thus, this invention provides a convenient system to gather information on the presence, identification, quantification and cytotoxic activity of eukaryotic and/or prokaryotic cells by determining their effect on the oxygen concentration of the media in which they are present.
摘要翻译： 本发明涉及基于其消耗溶解氧的能力来检测和评价真核细胞和/或原核细胞的代谢活性的方法。 该方法利用了发光检测系统，其利用某些化合物的发光发射对氧的存在的敏感性，其以浓度依赖性方式猝灭（减少）化合物的发光发射。 呼吸真核细胞和/或原核细胞将影响浸入其中的液体培养基的氧浓度。 因此，本发明提供了一种方便的系统，用于通过确定它们对存在的介质的氧浓度的影响来收集关于真核细胞和/或原核细胞的存在，鉴定，定量和细胞毒活性的信息。
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3.发明授权Method for determining the presence or absence of respiring cells on a three-dimensional scaffold 有权用于确定在三维支架上存在或不存在呼吸细胞的方法公开(公告)号：US06967086B2
公开(公告)日：2005-11-22
申请号：US10109475
申请日：2002-03-28
申请人： Richard David Guarino , John Jacob Hemperly , Catherine A. Spargo , Andrea Liebmann-Vinson , Mohammad A. Heidaran
发明人： Richard David Guarino , John Jacob Hemperly , Catherine A. Spargo , Andrea Liebmann-Vinson , Mohammad A. Heidaran
CPC分类号： C12Q1/04 , B33Y80/00 , C12Q1/18 , C12Q2304/44
摘要： The invention provides a method and apparatus for determining the presence or absence of respiring cells, involving combining a three-dimensional biomimetic scaffold and cells onto a sensor composition.
摘要翻译： 本发明提供了一种用于确定呼吸细胞的存在或不存在的方法和装置，其涉及将三维仿生支架和细胞组合到传感器组合物上。
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公开(公告)号：US06395506B1
公开(公告)日：2002-05-28
申请号：US09342720
申请日：1999-06-29
申请人： J. Bruce Pitner , John Jacob Hemperly , Richard D. Guarino , Magdalena Wodnicka , David T. Stitt , Gregory J. Burrell , Timothy G. Foley, Jr. , Patrick Shawn Beaty
发明人： J. Bruce Pitner , John Jacob Hemperly , Richard D. Guarino , Magdalena Wodnicka , David T. Stitt , Gregory J. Burrell , Timothy G. Foley, Jr. , Patrick Shawn Beaty
IPC分类号： C12Q118
CPC分类号： C12Q1/18 , C12Q1/04 , C12Q2304/44 , G06F19/00 , Y02A90/26
摘要： The present invention relates to methods for detection and evaluation of metabolic activity of eukaryotic and/or prokaryotic cells based upon their ability to consume dissolved oxygen. The methods utilize a luminescence detection system which makes use of the sensitivity of the luminescent emission of certain compounds to the presence of oxygen, which quenches (diminishes) the compound's luminescent emission in a concentration dependent manner. Respiring eukaryotic and/or prokaryotic cells will affect the oxygen concentration of a liquid medium in which they are immersed. Thus, this invention provides a convenient system to gather information on the presence, identification, quantification and cytotoxic activity of eukaryotic and/or prokaryotic cells by determining their effect on the oxygen concentration of the media in which they are present.
摘要翻译： 本发明涉及基于其消耗溶解氧的能力来检测和评价真核细胞和/或原核细胞的代谢活性的方法。 该方法利用了发光检测系统，其利用某些化合物的发光发射对氧的存在的敏感性，其以浓度依赖性方式猝灭（减少）化合物的发光发射。 呼吸真核细胞和/或原核细胞将影响浸入其中的液体培养基的氧浓度。 因此，本发明提供了一种方便的系统，用于通过确定它们对存在的介质的氧浓度的影响来收集关于真核细胞和/或原核细胞的存在，鉴定，定量和细胞毒活性的信息。
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公开(公告)号：US6143555A
公开(公告)日：2000-11-07
申请号：US219930
申请日：1998-12-23
CPC分类号： C12Q1/04 , C12Q1/001 , C12Q1/005 , C12Q2304/44 , Y10S435/81
摘要： An apparatus for detecting, identifying and measuring infectious disease causing microorganisms is described. The apparatus utilizes a plural medium arrangement in which a test liquid containing a microorganism is simultaneously introduced. Different media are utilized for the plural medium arrangement and at least one of the media can contain a respiration inhibiting material and/or other reagents such as antibiotics. Dissolved oxygen measurements of the media are taken as a measurement of microorganism respiration. Oxygen electrode signals within different media are compared and differential respiration variation rates are determined and identification signals produced. The identification signal resulting from the multiple respiration rate determinations is compared with known values to identify and measure the microorganism. The drug sensitivity of a microorganism is also judged when at least one of the media contains a drug for which the sensitivity of microorganism is to be determined.
摘要翻译： 描述了用于检测，识别和测量引起感染性微生物的传染病的装置。 该装置利用其中同时引入含有微生物的测试液的多种介质装置。 不同的培养基用于多种培养基布置，并且至少一种培养基可以含有呼吸抑制材料和/或其它试剂如抗生素。 将介质的溶解氧测量作为微生物呼吸的测量值。 比较不同介质中的氧电极信号，确定差异呼吸变化率，产生识别信号。 将由多次呼吸速率测定得到的识别信号与已知值进行比较，以鉴定和测量微生物。 当至少一种培养基含有确定微生物敏感性的药物时，也判断微生物的药物敏感性。
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公开(公告)号：US5567598A
公开(公告)日：1996-10-22
申请号：US25899
申请日：1993-03-03
CPC分类号： C12Q1/04 , C12Q1/18 , C12Q2304/44 , Y10S435/968 , Y10S436/80
摘要： This invention presents methods for detection and evaluation of metabolic activity of microorganisms based upon their ability to consume dissolved oxygen. The methods utilize a fluorescence detection system which makes use of the sensitivity of the fluorescent emission of certain compounds to the presence of oxygen, which quenches (diminishes) the compound's fluorescent emission in a concentration dependent manner. Respiring microorganisms will affect the oxygen concentration of a liquid medium in which they are immersed. Thus, this invention provides a convenient system to gather information on the presence, identification and metabolic activity of microorganisms by determining their effect on the oxygen concentration of the media in which they are present.
摘要翻译： 本发明提供了基于其消耗溶解氧的能力来检测和评估微生物的代谢活性的方法。 该方法利用荧光检测系统，其利用某些化合物的荧光发射对氧的存在的灵敏度，其以浓度依赖性方式猝灭（减少）化合物的荧光发射。 呼吸微生物将影响浸入其中的液体介质的氧浓度。 因此，本发明提供了一种方便的系统，通过确定它们对存在的微生物的存在，鉴定和代谢活性的影响，其对它们所存在的培养基的氧浓度的影响。
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公开(公告)号：US20050191613A1
公开(公告)日：2005-09-01
申请号：US10957802
申请日：2004-10-04
申请人： J. Bruce Pitner , John Jacob Hemperly , Richard Guarino , Magdalena Wodnicka , David Stitt , Gregory Burrell , Timothy Foley , Patrick Shawn Beaty
发明人： J. Bruce Pitner , John Jacob Hemperly , Richard Guarino , Magdalena Wodnicka , David Stitt , Gregory Burrell , Timothy Foley , Patrick Shawn Beaty
CPC分类号： C12Q1/18 , C12Q1/04 , C12Q2304/44 , G06F19/00 , Y02A90/26
摘要： The present invention relates to methods for detection and evaluation of metabolic activity of eukaryotic and/or prokaryotic cells based upon their ability to consume dissolved oxygen. The methods utilize a luminescence detection system which makes use of the sensitivity of the luminescent emission of certain compounds to the presence of oxygen, which quenches (diminishes) the compound's luminescent emission in a concentration dependent manner. Respiring eukaryotic and/or prokaryotic cells will affect the oxygen concentration of a liquid medium in which they are immersed. Thus, this invention provides a convenient system to gather information on the presence, identification, quantification and cytotoxic activity of eukaryotic and/or prokaryotic cells by determining their effect on the oxygen concentration of the media in which they are present.
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公开(公告)号：US20020192636A1
公开(公告)日：2002-12-19
申请号：US10109475
申请日：2002-03-28
发明人： Richard David Guarino , John Jacob Hemperly , Catherine A. Spargo , Andrea Liebmann-Vinson , Mohammad A. Heidaran
IPC分类号： C12Q001/00 , G01N033/53 , G01N033/567 , C12Q001/18
CPC分类号： C12Q1/04 , B33Y80/00 , C12Q1/18 , C12Q2304/44
摘要： The present invention relates to cell culture. In particular, this invention is directed to methods and apparatuses used to observe or quantitate cell proliferation in the presence of potential growth promoting molecules in a two or three dimensional architecture.
摘要翻译： 本发明涉及细胞培养。 特别地，本发明涉及用于在二维或三维结构中存在潜在生长促进分子的情况下观察或定量细胞增殖的方法和装置。
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公开(公告)号：US5876959A
公开(公告)日：1999-03-02
申请号：US638851
申请日：1996-04-29
CPC分类号： C12Q1/04 , C12Q2304/44
摘要： A method for detecting, identifying and measuring infectious disease causing microorganisms is described. The method utilizes a plural medium arrangement in which a test liquid containing a microorganism is simultaneously introduced. Different media are utilized for the plural medium arrangement and at least one of the media can contain a respiration inhibiting material and/or other reagents such as antibiotics. Dissolved oxygen measurements of the media are taken as a measurement of microorganism respiration. Oxygen electrode signals within different media are compared and differential respiration variation rates are determined and identification signals produced. The identification signal resulting from the multiple respiration rate determinations is compared with known values to identify and measure the microorganism. The drug sensitivity of a microorganism is also judged when at least one of the media contains a drug for which the sensitivity of microorganism is to be determined.
摘要翻译： 描述了用于检测，识别和测量引起感染性微生物的传染病的方法。 该方法利用了同时引入含有微生物的检测液的多种培养基配置。 不同的培养基用于多种培养基布置，并且至少一种培养基可以含有呼吸抑制材料和/或其它试剂如抗生素。 将介质的溶解氧测量作为微生物呼吸的测量值。 比较不同介质中的氧电极信号，确定差异呼吸变化率，产生识别信号。 将由多次呼吸速率测定得到的识别信号与已知值进行比较，以鉴定和测量微生物。 当至少一种培养基含有确定微生物敏感性的药物时，也判断微生物的药物敏感性。
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公开(公告)号：US06991918B2
公开(公告)日：2006-01-31
申请号：US09966505
申请日：2001-09-28
申请人： Steven C. Keith
发明人： Steven C. Keith
IPC分类号： C12Q1/22
CPC分类号： C12Q1/04 , C12Q1/18 , C12Q2304/44 , Y10T436/20
摘要： A method for detecting oxygen in a test sample is provided, the method involving exposing a test sample and a control sample to sensor compositions having a luminescent compound capable of generating a signal indicative of oxygen depletion. The method further involves determining the strengths of such signals for the test sample versus the control sample, and using the difference to determine whether oxygen in the test sample is being consumed.
摘要翻译： 提供了一种用于检测测试样品中的氧的方法，所述方法包括将测试样品和对照样品暴露于具有能够产生指示氧耗尽的信号的发光化合物的传感器组合物中。 该方法还包括确定测试样品与对照样品的这种信号的强度，并使用差异来确定测试样品中的氧是否正在消耗。
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