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    Attenuated African Swine Fever Virus Vaccine Based in the Deletion of MGF Genes
    5.
    发明申请
    Attenuated African Swine Fever Virus Vaccine Based in the Deletion of MGF Genes 有权
    基于去除MGF基因的非洲猪瘟病毒疫苗的减毒

    公开(公告)号：US20160130562A1

    公开(公告)日：2016-05-12

    申请号：US14537248

    申请日：2014-11-10

    申请人： The United States of America, as Represented by the Secretary of Agriculture ,  The University of Connecticut

    发明人： Manuel V. Borca ,  Lauren G. Holinka-Patterson ,  Vivian K. O'Donnell ,  Guillermo S. Risatti ,  Douglas Gladu

    IPC分类号： C12N7/00 ,  C12Q1/70 ,  A61K39/12

    CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K2039/5254 ,  A61K2039/552 ,  C12N2710/12034 ,  C12N2710/12062 ,  C12Q1/701

    摘要： African swine fever virus (ASFV) is the etiological agent of a contagious and often lethal viral disease of domestic pigs. Control of ASF has been hampered by the unavailability of vaccines. Experimental vaccines have been derived from naturally occurring, cell culture-adapted, or genetically modified live attenuated ASFVs; however, these vaccines are only successful when protecting against homologous viruses. Among viral genes reported to be involved in virulence are components of the multi gene family (MGF). Here we report the construction of a recombinant ΔMGF virus derived from the highly virulent ASFV Georgia 2007 (ASFV-G) isolate. In vivo, ASFV-G ΔMGF administered intramuscularly (IM) to swine at either 102 or 104 HAD50 are completely attenuated; the inoculated animals are completely asymptomatic. Animals infected with 102 or 104 HAD50 of ASFV-G ΔMGF are protected against the presentation of clinical disease when challenged at 28 days post infection with the virulent parental strain Georgia 2007.

    摘要翻译： 非洲猪瘟病毒（ASFV）是家养猪传染性和致死性病毒性疾病的病原体。 无法获得疫苗阻碍了ASF的控制。 实验疫苗已经衍生自天然存在的，细胞培养适应的或转基因活的减毒ASFV; 然而，这些疫苗仅在保护免受同源病毒时才成功。 据报道涉及毒力的病毒基因是多基因家族（MGF）的组成部分。 在这里，我们报告了从高毒性ASFV Georgia 2007（ASFV-G）分离株得到的重组＆Dgr; MGF病毒的构建。 在体内，在102或104HAD50下肌肉内（IM）施用的ASFV-G＆Dgr; MGF被完全减弱; 接种的动物完全无症状。 感染102或104个HAD50的ASFV-G＆Dgr; MGF的动物在感染后28天用有毒的亲本菌株Georgia 2007进行攻击时被保护免受临床疾病的表现。

    Attenuated African swine fever virus vaccine based in the deletion of MGF genes
    7.
    发明授权
    Attenuated African swine fever virus vaccine based in the deletion of MGF genes 有权
    减毒的非洲猪瘟病毒疫苗基于MGF基因的缺失

    公开(公告)号：US09528094B2

    公开(公告)日：2016-12-27

    申请号：US14537248

    申请日：2014-11-10

    申请人： The United States of America, as Represented by the Secretary of Agriculture ,  The University of Connecticut

    发明人： Manuel V. Borca ,  Lauren G. Holinka-Patterson ,  Vivian K. O'Donnell ,  Guillermo S. Risatti ,  Douglas Gladu

    IPC分类号： A61K39/12 ,  C12N7/00 ,  C12Q1/70

    CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K2039/5254 ,  A61K2039/552 ,  C12N2710/12034 ,  C12N2710/12062 ,  C12Q1/701

    摘要： African swine fever virus (ASFV) is the etiological agent of a contagious and often lethal viral disease of domestic pigs. Control of ASF has been hampered by the unavailability of vaccines. Experimental vaccines have been derived from naturally occurring, cell culture-adapted, or genetically modified live attenuated ASFVs; however, these vaccines are only successful when protecting against homologous viruses. Among viral genes reported to be involved in virulence are components of the multi gene family (MGF). Here we report the construction of a recombinant ΔMGF virus derived from the highly virulent ASFV Georgia 2007 (ASFV-G) isolate. In vivo, ASFV-G ΔMGF administered intramuscularly (IM) to swine at either 102 or 104 HAD50 are completely attenuated; the inoculated animals are completely asymptomatic. Animals infected with 102 or 104 HAD50 of ASFV-G ΔMGF are protected against the presentation of clinical disease when challenged at 28 days post infection with the virulent parental strain Georgia 2007.

    摘要翻译： 非洲猪瘟病毒（ASFV）是家养猪传染性和致死性病毒性疾病的病原体。 无法获得疫苗阻碍了ASF的控制。 实验疫苗已经衍生自天然存在的，细胞培养适应的或转基因活的减毒ASFV; 然而，这些疫苗仅在保护免受同源病毒时才成功。 据报道涉及毒力的病毒基因是多基因家族（MGF）的组成部分。 在这里，我们报告了源自高毒性ASFV Georgia 2007（ASFV-G）分离株的重组ΔMGF病毒的构建。 在体内，在102或104HAD50下肌肉内（IM）施用的猪ASFV-GΔMGF被完全减弱; 接种的动物完全无症状。 感染102或104个HAD50的ASFV-GΔMGF的动物在感染后28天用有毒的亲本菌株Georgia 2007进行攻击时被保护免受临床疾病的表现。