-
公开(公告)号：US20170196950A1
公开(公告)日：2017-07-13
申请号：US15316372
申请日：2015-06-05
发明人： Carolina LLANOS MUNOZ , Alexis Mikes KALERGIS PARRA , Fabian Alejandro VEGA TAPIA , Andy lgor TORRES BAEZA
IPC分类号： A61K39/00 , G01N33/569 , G01N33/68 , C12N5/0784
CPC分类号： A61K39/0008 , A61K35/15 , A61K2039/5154 , A61K2039/577 , C12N5/064 , C12N2500/92 , C12N2500/99 , C12N2501/04 , C12N2501/22 , C12N2501/2304 , C12N2501/999 , C12N2502/1114 , C12N2502/1192 , G01N33/56972 , G01N33/6869 , G01N2333/5412 , G01N2333/5434 , G01N2333/70503 , G01N2333/70532 , G01N2333/70539 , G01N2333/70578
摘要： The invention relates to a method for producing tolerogenic dendritic cells (tolDCs) with specific antigens, comprising the steps of: (a) culturing precursors of dendritic cells in an animal-serum-free medium, using cytokines, IL-4 and GM-CSF, in order to differentiate same in dendritic cells; (b) producing apoptotic cells; (c) culturing the dendritic cells obtained in step (b) in the presence of compounds having anti-inflammatory activity; (d) co-culturing the dendritic cells from step (d) with the apoptotic cells from step (c), such as to stimulate the endocytosis of the apoptotic cells by the dendritic cells; (e) and, by means of identification based on phenotypic evaluation, determining the production of tolerogenic dendritic cells (tolDCs) with specific antigens. The invention also relates to the tolDC cells produced with said method and to the use of said tolDCs with specific antigens in the production of a drug suitable for the treatment of systemic lupus erythematosus.
-
公开(公告)号：US08722402B2
公开(公告)日：2014-05-13
申请号：US13608579
申请日：2012-09-10
申请人： William L Warren , Heather Fahlenkamp , Russell Higbee , Anatoly Kachurin , Conan Li , Mike Nguyen , Robert Parkhill , Guzman Sanchez-Schmitz , Darrell J. Irvine , Gwendalyn J. Randolph , Nir Harcohen , Bruce Torbett
发明人： William L Warren , Heather Fahlenkamp , Russell Higbee , Anatoly Kachurin , Conan Li , Mike Nguyen , Robert Parkhill , Guzman Sanchez-Schmitz , Darrell J. Irvine , Gwendalyn J. Randolph , Nir Harcohen , Bruce Torbett
CPC分类号： G01N33/5008 , C12N5/0697 , C12N5/0698 , C12N2501/21 , C12N2502/094 , C12N2502/11 , C12N2502/1107 , C12N2502/1114 , C12N2502/1121 , C12N2502/1157 , C12N2502/1192 , C12N2502/1323 , C12N2502/28 , C12N2503/04 , C12N2513/00 , C12N2531/00 , C12N2533/50 , C12N2533/54 , C12N2535/10 , G01N33/5088 , G01N2500/10
摘要： The present invention relates to methods of constructing an integrated artificial immune system that comprises appropriate in vitro cellular and tissue constructs or their equivalents to mimic the normal tissues that interact with vaccines in mammals. The artificial immune system can be used to test the efficacy of vaccine candidates in vitro and thus, is useful to accelerate vaccine development and testing drug and chemical interaction with the immune system.
摘要翻译： 本发明涉及构建综合人造免疫系统的方法，其包括适当的体外细胞和组织构建体或其等同物以模拟与哺乳动物中的疫苗相互作用的正常组织。 人工免疫系统可用于测试疫苗候选物在体外的功效，因此可用于加速疫苗开发和测试与免疫系统的药物和化学相互作用。
-
公开(公告)号：US07855074B2
公开(公告)日：2010-12-21
申请号：US11116234
申请日：2005-04-28
申请人： William L. Warren , Heather Fahlenkamp , Russell Higbee , Anatoly Kachurin , Conan Li , Mike Nguyen , Robert Parkhill , Guzman Sanchez-Schmitz , Darrell J. Irvine , Gwendalyn J. Randolph , Nir Hacohen , Bruce Torbett
发明人： William L. Warren , Heather Fahlenkamp , Russell Higbee , Anatoly Kachurin , Conan Li , Mike Nguyen , Robert Parkhill , Guzman Sanchez-Schmitz , Darrell J. Irvine , Gwendalyn J. Randolph , Nir Hacohen , Bruce Torbett
IPC分类号： C12N5/08
CPC分类号： G01N33/5008 , C12N5/0697 , C12N5/0698 , C12N2501/21 , C12N2502/094 , C12N2502/11 , C12N2502/1107 , C12N2502/1114 , C12N2502/1121 , C12N2502/1157 , C12N2502/1192 , C12N2502/1323 , C12N2502/28 , C12N2503/04 , C12N2513/00 , C12N2531/00 , C12N2533/50 , C12N2533/54 , C12N2535/10 , G01N33/5088 , G01N2500/10
摘要： The present invention relates to methods of constructing an integrated artificial immune system that comprises appropriate in vitro cellular and tissue constructs or their equivalents to mimic the normal tissues that interact with vaccines in mammals. The artificial immune system can be used to test the efficacy of vaccine candidates in vitro and thus, is useful to accelerate vaccine development and testing drug and chemical interaction with the immune system.
摘要翻译： 本发明涉及构建综合人造免疫系统的方法，其包括适当的体外细胞和组织构建体或其等同物以模拟与哺乳动物中的疫苗相互作用的正常组织。 人工免疫系统可用于测试疫苗候选物在体外的功效，因此可用于加速疫苗开发和测试与免疫系统的药物和化学相互作用。
-
公开(公告)号：US20060270029A1
公开(公告)日：2006-11-30
申请号：US11375033
申请日：2006-03-15
申请人： William Warren , Robert Parkhill , Michael Nguyen , Guzman Sanchez-Schmitz , Heather Fahlenkamp , Russell Higbee , Donald Drake , Anatoly Kachurin , David Moe
发明人： William Warren , Robert Parkhill , Michael Nguyen , Guzman Sanchez-Schmitz , Heather Fahlenkamp , Russell Higbee , Donald Drake , Anatoly Kachurin , David Moe
CPC分类号： C12N5/0697 , B01L3/5027 , C12N2501/21 , C12N2502/094 , C12N2502/11 , C12N2502/1107 , C12N2502/1114 , C12N2502/1121 , C12N2502/1192 , C12N2502/28 , C12N2503/02 , C12N2503/04 , C12N2531/00 , C12N2533/50 , C12N2533/54 , C12N2535/10 , G01N33/5082 , G01N33/5088
摘要： The present invention relates to methods of constructing an integrated artificial immune system that comprises appropriate in vitro cellular and tissue constructs or their equivalents to mimic the normal tissues that interact with vaccines in mammals. The artificial immune system can be used to test the efficacy of vaccine candidates in vitro and thus, is useful to accelerate vaccine development and testing drug and chemical interactions with the immune system.
摘要翻译： 本发明涉及构建综合人造免疫系统的方法，其包括适当的体外细胞和组织构建体或其等同物以模拟与哺乳动物中的疫苗相互作用的正常组织。 人造免疫系统可用于测试疫苗候选物在体外的功效，因此可用于加速疫苗开发和测试与免疫系统的药物和化学相互作用。
-
公开(公告)号：US09625444B2
公开(公告)日：2017-04-18
申请号：US14221018
申请日：2014-03-20
发明人： William L Warren , Heather Fahlenkamp , Russell Higbee , Anatoly Kachurin , Conan Li , Mike Nguyen , Robert Parkhill , Guzman Sanchez-Schmitz , Darrell J. Irvine , Gwendalyn J. Randolph , Nir Hacohen , Bruce Torbett
CPC分类号： G01N33/5008 , C12N5/0697 , C12N5/0698 , C12N2501/21 , C12N2502/094 , C12N2502/11 , C12N2502/1107 , C12N2502/1114 , C12N2502/1121 , C12N2502/1157 , C12N2502/1192 , C12N2502/1323 , C12N2502/28 , C12N2503/04 , C12N2513/00 , C12N2531/00 , C12N2533/50 , C12N2533/54 , C12N2535/10 , G01N33/5088 , G01N2500/10
摘要： The present invention relates to methods of constructing an integrated artificial immune system that comprises appropriate in vitro cellular and tissue constructs or their equivalents to mimic the normal tissues that interact with vaccines in mammals. The artificial immune system can be used to test the efficacy of vaccine candidates in vitro and thus, is useful to accelerate vaccine development and testing drug and chemical interaction with the immune system.
-
公开(公告)号：US08288159B2
公开(公告)日：2012-10-16
申请号：US12725698
申请日：2010-03-17
申请人： William L Warren , Heather Fahlenkamp , Russell Higbee , Anatoly Kachurin , Conan Li , Mike Nguyen , Robert Parkhill , Guzman Sanchez-Schmitz , Darrell J. Irvine , Gwendalyn J. Randolph , Nir Harcohen , Bruce Torbett
发明人： William L Warren , Heather Fahlenkamp , Russell Higbee , Anatoly Kachurin , Conan Li , Mike Nguyen , Robert Parkhill , Guzman Sanchez-Schmitz , Darrell J. Irvine , Gwendalyn J. Randolph , Nir Harcohen , Bruce Torbett
CPC分类号： G01N33/5008 , C12N5/0697 , C12N5/0698 , C12N2501/21 , C12N2502/094 , C12N2502/11 , C12N2502/1107 , C12N2502/1114 , C12N2502/1121 , C12N2502/1157 , C12N2502/1192 , C12N2502/1323 , C12N2502/28 , C12N2503/04 , C12N2513/00 , C12N2531/00 , C12N2533/50 , C12N2533/54 , C12N2535/10 , G01N33/5088 , G01N2500/10
摘要： The present invention relates to methods of constructing an integrated artificial immune system that comprises appropriate in vitro cellular and tissue constructs or their equivalents to mimic the normal tissues that interact with vaccines in mammals. The artificial immune system can be used to test the efficacy of vaccine candidates in vitro and thus, is useful to accelerate vaccine development and testing drug and chemical interaction with the immune system.
摘要翻译： 本发明涉及构建综合人造免疫系统的方法，其包括适当的体外细胞和组织构建体或其等同物以模拟与哺乳动物中的疫苗相互作用的正常组织。 人工免疫系统可用于测试疫苗候选物在体外的功效，因此可用于加速疫苗开发和测试与免疫系统的药物和化学相互作用。
-
公开(公告)号：US08030070B2
公开(公告)日：2011-10-04
申请号：US11471514
申请日：2006-06-21
申请人： Guzman Sanchez-Schmitz , Russell Higbee , Heather Fahlenkamp , Darrell J. Irvine , William L. Warren , Donald Drake, III
发明人： Guzman Sanchez-Schmitz , Russell Higbee , Heather Fahlenkamp , Darrell J. Irvine , William L. Warren , Donald Drake, III
IPC分类号： C12N5/0781 , C12N5/0783 , C12N5/0784
CPC分类号： C12N5/0639 , C12N5/0651 , C12N5/0697 , C12N2501/21 , C12N2502/1107 , C12N2502/1114 , C12N2502/1121 , C12N2502/1192 , C12N2502/28 , C12N2503/04 , C12N2533/50 , C12N2533/54 , C12N2535/10 , G01N33/5047
摘要： The present invention relates to methods of constructing an integrated artificial immune system that comprises appropriate in vitro cellular and tissue constructs or their equivalents to mimic the normal tissues that interact with vaccines in mammals. The artificial immune system can be used to test the efficacy of vaccine candidates in vitro and thus, is useful to accelerate vaccine development and testing drug and chemical interactions with the immune system.
摘要翻译： 本发明涉及构建综合人造免疫系统的方法，其包括适当的体外细胞和组织构建体或其等同物以模拟与哺乳动物中的疫苗相互作用的正常组织。 人造免疫系统可用于测试疫苗候选物在体外的功效，因此可用于加速疫苗开发和测试与免疫系统的药物和化学相互作用。
-
公开(公告)号：US20110171689A1
公开(公告)日：2011-07-14
申请号：US12725698
申请日：2010-03-17
申请人： William L. Warren , Heather Fahlenkamp , Russell Higbee , Anatoly Kachurin , Conan Li , Mike Nguyen , Robert Parkhill , Guzman Sanchez-Schmitz , Darrell J. Irvine , Gwendalyn J. Randolph , Nir Hacohen , Bruce Torbett
发明人： William L. Warren , Heather Fahlenkamp , Russell Higbee , Anatoly Kachurin , Conan Li , Mike Nguyen , Robert Parkhill , Guzman Sanchez-Schmitz , Darrell J. Irvine , Gwendalyn J. Randolph , Nir Hacohen , Bruce Torbett
IPC分类号： C12P21/00
CPC分类号： G01N33/5008 , C12N5/0697 , C12N5/0698 , C12N2501/21 , C12N2502/094 , C12N2502/11 , C12N2502/1107 , C12N2502/1114 , C12N2502/1121 , C12N2502/1157 , C12N2502/1192 , C12N2502/1323 , C12N2502/28 , C12N2503/04 , C12N2513/00 , C12N2531/00 , C12N2533/50 , C12N2533/54 , C12N2535/10 , G01N33/5088 , G01N2500/10
摘要： The present invention relates to methods of constructing an integrated artificial immune system that comprises appropriate in vitro cellular and tissue constructs or their equivalents to mimic the normal tissues that interact with vaccines in mammals. The artificial immune system can be used to test the efficacy of vaccine candidates in vitro and thus, is useful to accelerate vaccine development and testing drug and chemical interaction with the immune system.
摘要翻译： 本发明涉及构建综合人造免疫系统的方法，其包括适当的体外细胞和组织构建体或其等同物以模拟与哺乳动物中的疫苗相互作用的正常组织。 人工免疫系统可用于测试疫苗候选物在体外的功效，因此可用于加速疫苗开发和测试与免疫系统的药物和化学相互作用。
-
公开(公告)号：US20070218054A1
公开(公告)日：2007-09-20
申请号：US11642938
申请日：2006-12-21
申请人： Selva Sukumar , Mohey Eldin El Shikh , John Tew , Guzman Sanchez-Schmitz , Donald Drake , Luis Mosquera , Eric Mishkin , Anatoly Kachurin , Russell Higbee , Conan Li , William Warren , Heather Fahlenkamp
发明人： Selva Sukumar , Mohey Eldin El Shikh , John Tew , Guzman Sanchez-Schmitz , Donald Drake , Luis Mosquera , Eric Mishkin , Anatoly Kachurin , Russell Higbee , Conan Li , William Warren , Heather Fahlenkamp
IPC分类号： A61K39/395 , G01N33/567 , C12P21/04 , C12N5/08
CPC分类号： C07K16/44 , C12N5/0639 , C12N5/0651 , C12N5/0697 , C12N2501/052 , C12N2501/21 , C12N2501/2304 , C12N2502/11 , C12N2502/1107 , C12N2502/1114 , C12N2502/1121 , C12N2502/1192 , C12N2502/28 , C12N2503/04 , C12N2533/50 , C12N2533/54 , C12N2533/92 , C12N2535/10 , G01N33/5047 , G01N33/5076 , G01N33/5088
摘要： The present invention incorporates germinal centers (GCs) into three-dimensional (3D) engineered tissue constructs (ETCs). In an embodiment, we have incorporated the GC in the design of an artificial immune system (AIS) to examine immune responses to vaccines and other compounds. Development of an in vitro GC adds functionality to an AIS, in that it enables generation of an in vitro human humoral response by human B lymphocytes that is accurate and reproducible, without using human subjects. The invention also permits evaluation of, for example, vaccines, allergens, and immunogens, and activation of human B cells specific for a given antigen, which can then be used to generate human antibodies. In an embodiment of the present invention the function of the in vitro GC is enhanced by placing FDCs and other immune cells in a 3D ETC; FDCs appear more effective over a longer time (antibody production is sustained for up to about 14 days.
摘要翻译： 本发明将生发中心（GC）纳入三维（3D）工程组织构建体（ETC）中。 在一个实施方案中，我们已经将GC并入人造免疫系统（AIS）的设计中，以检查对疫苗和其他化合物的免疫应答。 体外GC的开发为AIS增加了功能，因为它能够在不使用人类受试者的情况下产生准确和可重复的人B淋巴细胞的体外人体液反应。 本发明还允许评估例如疫苗，过敏原和免疫原，以及对给定抗原特异性的人B细胞的活化，然后可以将其用于产生人抗体。 在本发明的一个实施方案中，通过将FDC和其他免疫细胞放置在3D ETC中来增强体外GC的功能; FDC在更长时间内看起来更有效（抗体产生持续长达约14天。
-
公开(公告)号：US20050282148A1
公开(公告)日：2005-12-22
申请号：US11116234
申请日：2005-04-28
申请人： William Warren , Heather Fahlenkamp , Russell Higbee , Anatoly Kachurin , Conan Li , Mike Nguyen , Robert Parkhill , Guzman Sanchez-Schmitz , Darrell Irvine , Gwendalyn Randolph , Nir Hacohen , Bruce Torbett
发明人： William Warren , Heather Fahlenkamp , Russell Higbee , Anatoly Kachurin , Conan Li , Mike Nguyen , Robert Parkhill , Guzman Sanchez-Schmitz , Darrell Irvine , Gwendalyn Randolph , Nir Hacohen , Bruce Torbett
CPC分类号： G01N33/5008 , C12N5/0697 , C12N5/0698 , C12N2501/21 , C12N2502/094 , C12N2502/11 , C12N2502/1107 , C12N2502/1114 , C12N2502/1121 , C12N2502/1157 , C12N2502/1192 , C12N2502/1323 , C12N2502/28 , C12N2503/04 , C12N2513/00 , C12N2531/00 , C12N2533/50 , C12N2533/54 , C12N2535/10 , G01N33/5088 , G01N2500/10
摘要： The present invention relates to methods of constructing an integrated artificial immune system that comprises appropriate in vitro cellular and tissue constructs or their equivalents to mimic the normal tissues that interact with vaccines in mammals. The artificial immune system can be used to test the efficacy of vaccine candidates in vitro and thus, is useful to accelerate vaccine development and testing drug and chemical interaction with the immune system.
摘要翻译： 本发明涉及构建综合人造免疫系统的方法，其包括适当的体外细胞和组织构建体或其等同物以模拟与哺乳动物中的疫苗相互作用的正常组织。 人工免疫系统可用于测试疫苗候选物在体外的功效，因此可用于加速疫苗开发和测试与免疫系统的药物和化学相互作用。
-
-
-
-
-
-
-
-
-
搜索结果
34 条记录 (耗时 0.031 秒)
