公开(公告)号：US20180156816A1

公开(公告)日：2018-06-07

申请号：US15888455

申请日：2018-02-05

Applicant: Xiujuan Hao ,  Isaac A Angres

Inventor： Xiujuan Hao ,  Isaac A Angres

IPC: G01N33/68 ,  C07H19/16 ,  C07K16/44 ,  G01N33/574 ,  A61K31/7076

CPC classification number: G01N33/6812 ,  A61K31/7076 ,  C07H19/16 ,  C07K16/44 ,  C07K2317/20 ,  C07K2317/33 ,  G01N33/57484 ,  G01N2440/12 ,  G01N2800/105 ,  G01N2800/7033 ,  Y02A50/423

Abstract: The invention provides a method of detecting the presence, absence or severity of a disease in a patient wherein said disease is accompanied by decreased level of S-adenosylmethionine, or increased level of S-adenosylhomocysterine, or reduced methylation index comprising: identifying any individual or a patient that is suspected of having said disease or is at risk of having said disease; obtaining a biological sample from said patient; determining the level of SAM in said biological sample using an antibody derived from a hapten analog of SAM, SAH; and correlating the levels of SAM, SAH and MI in said biological sample with the presence, absence, or severity of said disease. The invention also provides methods for determining methylation index in biological fluids which is indicative of the health status of an individual. Additionally, the invention includes colloidal gold test strips and homogenous enzyme immunoassays which are useful for determining S-adenosylmethionine and S-adenosylhomocysteine.

公开(公告)号：US09885723B2

公开(公告)日：2018-02-06

申请号：US14732775

申请日：2015-06-07

Applicant: Xiujuan Hao ,  Isaac A Angres

Inventor： Xiujuan Hao ,  Isaac A Angres

IPC: A61K31/7076 ,  G01N33/68 ,  C07H19/16 ,  C07K16/44 ,  G01N33/574

CPC classification number: G01N33/6812 ,  A61K31/7076 ,  C07H19/16 ,  C07K16/44 ,  C07K2317/20 ,  C07K2317/33 ,  G01N33/57484 ,  G01N2440/12 ,  G01N2800/105 ,  G01N2800/7033 ,  Y02A50/423

Abstract: The invention provides a method of detecting the presence, absence or severity of a disease in a patient wherein said disease is accompanied by decreased level of S-adenosylmethionine, or increased level of S-adenosylhomocysterine, or reduced methylation index comprising: identifying any individual or a patient that is suspected of having said disease or is at risk of having said disease; obtaining a biological sample from said patient; determining the level of SAM in said biological sample using an antibody derived from a hapten analog of SAM, SAH; and correlating the levels of SAM, SAH and MI in said biological sample with the presence, absence, or severity of said disease. The invention also provides methods for determining methylation index in biological fluids which is indicative of the health status of an individual. Additionally, the invention includes colloidal gold test strips and homogenous enzyme immunoassays which are useful for determining S-adenosylmethionine and S-adenosylhomocysteine.

公开(公告)号：US10656159B2

公开(公告)日：2020-05-19

申请号：US15888455

申请日：2018-02-05

Applicant: Xiujuan Hao ,  Isaac A Angres

Inventor： Xiujuan Hao ,  Isaac A Angres

IPC: G01N33/68 ,  C07K16/44 ,  A61K31/7076 ,  C07H19/16 ,  G01N33/574

Abstract: The invention provides a method of detecting the presence, absence or severity of a disease in a patient wherein said disease is accompanied by decreased level of S-adenosylmethionine, or increased level of S-adenosylhomocysterine, or reduced methylation index comprising: identifying any individual or a patient that is suspected of having said disease or is at risk of having said disease; obtaining a biological sample from said patient; determining the level of SAM in said biological sample using an antibody derived from a hapten analog of SAM, SAH; and correlating the levels of SAM, SAH and MI in said biological sample with the presence, absence, or severity of said disease. The invention also provides methods for determining methylation index in biological fluids which is indicative of the health status of an individual. Additionally, the invention includes colloidal gold test strips and homogenous enzyme immunoassays which are useful for determining S-adenosylmethionine and S-adenosylhomocysteine.

公开(公告)号：US09958447B2

公开(公告)日：2018-05-01

申请号：US14457099

申请日：2014-08-11

Applicant: Xiujuan Hao ,  Isaac A Angres

Inventor： Xiujuan Hao ,  Isaac A Angres

IPC: C07K16/44 ,  G01N33/574 ,  C07H19/16 ,  C07D473/02 ,  C07D493/04 ,  C07D519/00 ,  G01N33/53

CPC classification number: G01N33/57484 ,  C07D473/02 ,  C07D493/04 ,  C07D519/00 ,  C07H19/16 ,  C07K16/44 ,  C07K2317/20 ,  C07K2317/33 ,  G01N2800/7033

Abstract: The invention provides a method of detecting the presence or absence of a disease in a patient wherein said disease is accompanied by deficient levels of S-adenosylmethionine comprising: identifying a patient that is suspected of having said disease or is at risk of having said disease; obtaining a biological sample from said patient; determining the level of S-adenosylmethionine in said biological sample using an antibody derived from a hapten analog of S-adenosylmethionine; and correlating the level of S-adenosylmethionine in said biological sample with the presence, absence, or severity of said disease. The invention also provides methods for measuring SAH which is used to determine the methylation index (ratio of SAM/SAH) in biological fluids which is indicative of the health status of an individual. Additionally, the invention includes test strips which are useful for determining S-adenosylmethionine and S-adenosylhomocysteine.

公开(公告)号：US09877982B2

公开(公告)日：2018-01-30

申请号：US14218928

申请日：2014-03-18

Applicant: Xiujuan Hao ,  Isaac A Angres

Inventor： Xiujuan Hao ,  Isaac A Angres

IPC: G01N33/574 ,  A61K31/7076

CPC classification number: A61K31/7076 ,  G01N33/57484 ,  G01N2800/7033

Abstract: A method of detecting the presence or absence of a disease in a patient wherein said disease is accompanied by deficient levels of S-adenosylmethionine comprising: identifying a patient that is suspected of having said disease or is at risk of having said disease; obtaining a biological sample from said patient; determining the level of S-adenosylmethionine in said biological sample using an antibody derived from a hapten analog of S-adenosylmethionine; and correlating the level of S-adenosylmethionine in said biological sample with the presence or absence of said disease. The invention also provides methods for measuring SAH which is used to determine the methylation index (ratio of SAM/SAH) in biological fluids which is indicative of the health status of an individual.

公开(公告)号：US20160041154A1

公开(公告)日：2016-02-11

申请号：US14732775

申请日：2015-06-07

Applicant: Xiujuan Hao ,  Isaac A. Angres

Inventor： Xiujuan Hao ,  Isaac A. Angres

IPC: G01N33/53

CPC classification number: G01N33/6812 ,  A61K31/7076 ,  C07H19/16 ,  C07K16/44 ,  C07K2317/20 ,  C07K2317/33 ,  G01N33/57484 ,  G01N2440/12 ,  G01N2800/105 ,  G01N2800/7033 ,  Y02A50/423

Abstract: The invention provides a method of detecting the presence, absence or severity of a disease in a patient wherein said disease is accompanied by decreased level of S-adenosylmethionine, or increased level of S-adenosylhomocysterine, or reduced methylation index comprising: identifying any individual or a patient that is suspected of having said disease or is at risk of having said disease; obtaining a biological sample from said patient; determining the level of SAM in said biological sample using an antibody derived from a hapten analog of SAM, SAH; and correlating the levels of SAM, SAH and MI in said biological sample with the presence, absence, or severity of said disease. The invention also provides methods for determining methylation index in biological fluids which is indicative of the health status of an individual. Additionally, the invention includes colloidal gold test strips and homogenous enzyme immunoassays which are useful for determining S-adenosylmethionine and S-adenosylhomocysteine.

Abstract translation: 本发明提供了一种检测患者疾病的存在，不存在或严重性的方法，其中所述疾病伴随着S-腺苷甲硫氨酸水平的降低或S-腺苷神经迟缓的水平升高或降低的甲基化指数，包括：鉴定任何个体或 怀疑患有该疾病或有患上述疾病风险的患者; 从所述患者获得生物样品; 使用衍生自SAM，SAH的半抗原类似物的抗体确定所述生物样品中SAM的水平; 并将所述生物样品中SAM，SAH和MI的水平与所述疾病的存在，不存在或严重性相关联。 本发明还提供了用于测定生物流体中的甲基化指数的方法，其指示个体的健康状况。 此外，本发明包括胶体金测试条和用于测定S-腺苷甲硫氨酸和S-腺苷高半胱氨酸的均质酶免疫测定。

公开(公告)号：US20150268231A1

公开(公告)日：2015-09-24

申请号：US14457099

申请日：2014-08-11

Applicant: Xiujuan Hao ,  Isaac A. Angres

Inventor： Xiujuan Hao ,  Isaac A. Angres

IPC: G01N33/53 ,  G01N33/564 ,  G01N33/574 ,  C07H19/16 ,  C07K16/44

CPC classification number: G01N33/57484 ,  C07D473/02 ,  C07D493/04 ,  C07D519/00 ,  C07H19/16 ,  C07K16/44 ,  C07K2317/20 ,  C07K2317/33 ,  G01N2800/7033

Abstract: The invention provides a method of detecting the presence or absence of a disease in a patient wherein said disease is accompanied by deficient levels of S-adenosylmethionine comprising: identifying a patient that is suspected of having said disease or is at risk of having said disease; obtaining a biological sample from said patient; determining the level of S-adenosylmethionine in said biological sample using an antibody derived from a hapten analog of S-adenosylmethionine; and correlating the level of S-adenosylmethionine in said biological sample with the presence, absence, or severity of said disease. The invention also provides methods for measuring SAH which is used to determine the methylation index (ratio of SAM/SAH) in biological fluids which is indicative of the health status of an individual. Additionally, the invention includes test strips which are useful for determining S-adenosylmethionine and S-adenosylhomocysteine.

Abstract translation: 本发明提供了一种检测患者疾病存在或不存在的方法，其中所述疾病伴随有S-腺苷甲硫氨酸水平不足的疾病，其包括：鉴定疑似患有该疾病或有风险患有该疾病的患者; 从所述患者获得生物样品; 使用衍生自S-腺苷甲硫氨酸的半抗原类似物的抗体测定所述生物样品中S-腺苷甲硫氨酸的水平; 并将所述生物样品中S-腺苷甲硫氨酸的水平与所述疾病的存在，不存在或严重性相关联。 本发明还提供了用于测量SAH的方法，其用于确定生物流体中的甲基化指数（SAM / SAH的比例），其指示个体的健康状况。 此外，本发明包括可用于测定S-腺苷甲硫氨酸和S-腺苷高半胱氨酸的测试条。

公开(公告)号：US20140271945A1

公开(公告)日：2014-09-18

申请号：US14218928

申请日：2014-03-18

Applicant: Xiujuan Hao ,  Isaac A. Angres

Inventor： Xiujuan Hao ,  Isaac A. Angres

IPC: G01N33/574 ,  A61K31/7076

CPC classification number: A61K31/7076 ,  G01N33/57484 ,  G01N2800/7033

Abstract: A method of detecting the presence or absence of a disease in a patient wherein said disease is accompanied by deficient levels of S-adenosylmethionine comprising: identifying a patient that is suspected of having said disease or is at risk of having said disease; obtaining a biological sample from said patient; determining the level of S-adenosylmethionine in said biological sample using an antibody derived from a hapten analog of S-adenosylmethionine; and correlating the level of S-adenosylmethionine in said biological sample with the presence or absence of said disease. The invention also provides methods for measuring SAH which is used to determine the methylation index (ratio of SAM/SAH) in biological fluids which is indicative of the health status of an individual.

Abstract translation: 一种检测患者疾病存在或不存在的方法，其中所述疾病伴随有S-腺苷甲硫氨酸水平不足的疾病，包括：鉴定疑似患有所述疾病或有风险患有该疾病的患者; 从所述患者获得生物样品; 使用衍生自S-腺苷甲硫氨酸的半抗原类似物的抗体测定所述生物样品中S-腺苷甲硫氨酸的水平; 并将所述生物样品中S-腺苷甲硫氨酸的水平与存在或不存在所述疾病相关联。 本发明还提供了用于测量SAH的方法，其用于确定生物流体中的甲基化指数（SAM / SAH的比例），其指示个体的健康状况。

公开(公告)号：US11041856B2

公开(公告)日：2021-06-22

申请号：US16120418

申请日：2018-09-03

Applicant: Xiujuan Hao ,  Chaoyi Deng

Inventor： Xiujuan Hao ,  Chaoyi Deng

IPC: G01N33/543 ,  G01N33/533 ,  A61K49/00 ,  C07K16/44

Abstract: The invention provides immunochromatographic test strips and methods and systems or kits for detecting and quantifying S-Adenosylmethionine (SAM), S-Adenosylhomocysteine (SAH) and Homocysteine (HCy) in a sample, comprising: (a) making fluorophore conjugated antibodies; (b) immobilizing SAM, SAH and HCy on a solid support; (c) providing a sample, combining said sample with a conjugate selected from the group consisting of lanthanide chelate conjugates and quantum dot conjugates (QD) with anti-SAM, anti-SAH or anti-HCy, wherein said combining is performed under conditions that allow formation of a competitive complex comprising said conjugate, said SAM, SAH or HCy on the solid support and SAM, SAH or HCy in a sample when present; and (d) detecting the presence of the complex, if present, by monitoring a spectral emission mediated by the fluorescent conjugates in the complex, wherein the emission indicates the presence and quantity of SAM, SAH or HCy in the sample.

公开(公告)号：US20180169128A1

公开(公告)日：2018-06-21

申请号：US15881817

申请日：2018-01-29

Applicant: Xiujuan Hao ,  Isaac Angres

Inventor： Xiujuan Hao ,  Isaac Angres

IPC: A61K31/7076 ,  G01N33/574

CPC classification number: A61K31/7076 ,  G01N33/57484 ,  G01N2800/7033

Abstract: A method of detecting the presence or absence of a disease in a patient wherein said disease is accompanied by deficient levels of S-adenosylmethionine comprising: identifying a patient that is suspected of having said disease or is at risk of having said disease; obtaining a biological sample from said patient; determining the level of S-adenosylmethionine in said biological sample using an antibody derived from a hapten analog of S-adenosylmethionine; and correlating the level of S-adenosylmethionine in said biological sample with the presence or absence of said disease. The invention also provides methods for measuring SAH which is used to determine the methylation index (ratio of SAM/SAH) in biological fluids which is indicative of the health status of an individual.