公开(公告)号：US11041856B2

公开(公告)日：2021-06-22

申请号：US16120418

申请日：2018-09-03

Applicant: Xiujuan Hao ,  Chaoyi Deng

Inventor： Xiujuan Hao ,  Chaoyi Deng

IPC: G01N33/543 ,  G01N33/533 ,  A61K49/00 ,  C07K16/44

Abstract: The invention provides immunochromatographic test strips and methods and systems or kits for detecting and quantifying S-Adenosylmethionine (SAM), S-Adenosylhomocysteine (SAH) and Homocysteine (HCy) in a sample, comprising: (a) making fluorophore conjugated antibodies; (b) immobilizing SAM, SAH and HCy on a solid support; (c) providing a sample, combining said sample with a conjugate selected from the group consisting of lanthanide chelate conjugates and quantum dot conjugates (QD) with anti-SAM, anti-SAH or anti-HCy, wherein said combining is performed under conditions that allow formation of a competitive complex comprising said conjugate, said SAM, SAH or HCy on the solid support and SAM, SAH or HCy in a sample when present; and (d) detecting the presence of the complex, if present, by monitoring a spectral emission mediated by the fluorescent conjugates in the complex, wherein the emission indicates the presence and quantity of SAM, SAH or HCy in the sample.

公开(公告)号：US20180372735A1

公开(公告)日：2018-12-27

申请号：US16120418

申请日：2018-09-03

Applicant: Xiujuan Hao ,  Chaoyi Deng

Inventor： Xiujuan Hao ,  Chaoyi Deng

IPC: G01N33/543 ,  G01N33/533

Abstract: The invention provides immunochromatographic test strips and methods and systems or kits for detecting and quantifying S-Adenosylmethionine (SAM), S-Adenosylhomocysteine (SAH) and Homocysteine (HCy) in a sample, comprising: (a) making fluorophore conjugated antibodies; (b) immobilizing SAM, SAH and HCy on a solid support; (c) providing a sample, combining said sample with a conjugate selected from the group consisting of lanthanide chelate conjugates and quantum dot conjugates (QD) with anti-SAM, anti-SAH or anti-HCy, wherein said combining is performed under conditions that allow formation of a competitive complex comprising said conjugate, said SAM, SAH or HCy on the solid support and SAM, SAH or HCy in a sample when present; and (d) detecting the presence of the complex, if present, by monitoring a spectral emission mediated by the fluorescent conjugates in the complex, wherein the emission indicates the presence and quantity of SAM, SAH or HCy in the sample.

公开(公告)号：US20160349251A1

公开(公告)日：2016-12-01

申请号：US15164856

申请日：2016-05-25

Applicant: Xiujuan Hao ,  Junhua Wu ,  Chaoyi Deng

Inventor： Xiujuan Hao ,  Junhua Wu ,  Chaoyi Deng

IPC: G01N33/543

CPC classification number: G01N33/54373 ,  A61K49/0058 ,  C07K16/44 ,  G01N33/533 ,  G01N2333/4737 ,  G01N2800/50

Abstract: The invention provides immunochromatographic test strips and methods for detecting and quantifying S-Adenosylmethionine (SAM), S-Adenosylhomocysteine (SAH) and Homocysteine (HCy) in a sample, comprising: (a) making fluorophore conjugated antibodies; (b) immobilizing SAM, SAH and HCy on a solid support; (c) providing a sample, combining said sample with a conjugate selected from the group consisting of lanthanide chelate conjugates and quantum dot conjugates (QD) with anti-SAM, anti-SAH or anti-HCy, wherein said combining is performed under conditions that allow formation of a competitive complex comprising said conjugate, said SAM, SAH or HCy on the solid support and SAM, SAH or HCy in a sample when present; and (d) detecting the presence of the complex, if present, by monitoring a spectral emission mediated by the fluorescent conjugates in the complex, wherein the emission indicates the presence and quantity of SAM, SAH or HCy in the sample.

Abstract translation: 本发明提供免疫色谱测试条和用于检测和定量样品中S-腺苷甲硫氨酸（SAM），S-腺苷高半胱氨酸（SAH）和同型半胱氨酸（HCy）的方法，包括：（a）制备荧光团缀合的抗体; （b）将SAM，SAH和HCY固定在固体支持物上; （c）提供样品，将所述样品与选自镧系元素螯合物缀合物和量子点缀合物（QD）的共轭物与抗SAM，抗SAH或抗-HCi组合，其中所述组合在以下条件下进行： 允许在固体支持物上形成包含所述缀合物，所述SAM，SAH或HCy的竞争性复合物，当存在时，样品中的SAM，SAH或HCy; 和（d）通过监测由络合物中的荧光共轭物介导的光谱发射来检测复合物的存在（如果存在），其中发射指示样品中SAM，SAH或HCy的存在和数量。

公开(公告)号：US10067126B2

公开(公告)日：2018-09-04

申请号：US15164856

申请日：2016-05-25

Applicant: Xiujuan Hao ,  Junhua Wu ,  Chaoyi Deng

Inventor： Xiujuan Hao ,  Junhua Wu ,  Chaoyi Deng

IPC: G01N33/533 ,  C07K16/44 ,  G01N33/543 ,  A61K49/00

Abstract: The invention provides immunochromatographic test strips and methods for detecting and quantifying S-Adenosylmethionine (SAM), S-Adenosylhomocysteine (SAH) and Homocysteine (HCy) in a sample, comprising: (a) making fluorophore conjugated antibodies; (b) immobilizing SAM, SAH and HCy on a solid support; (c) providing a sample, combining said sample with a conjugate selected from the group consisting of lanthanide chelate conjugates and quantum dot conjugates (QD) with anti-SAM, anti-SAH or anti-HCy, wherein said combining is performed under conditions that allow formation of a competitive complex comprising said conjugate, said SAM, SAH or HCy on the solid support and SAM, SAH or HCy in a sample when present; and (d) detecting the presence of the complex, if present, by monitoring a spectral emission mediated by the fluorescent conjugates in the complex, wherein the emission indicates the presence and quantity of SAM, SAH or HCy in the sample.