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公开(公告)号：US08982206B2
公开(公告)日：2015-03-17
申请号：US13439958
申请日：2012-04-05
申请人： Valerica Raicu , Michael Stoneman
发明人： Valerica Raicu , Michael Stoneman
CPC分类号： G01J3/2803 , G01J3/027 , G01J3/0294 , G01J3/06 , G01J3/4406 , G01N15/1434 , G01N15/1463 , G01N15/1475 , G01N2015/1006 , G02B21/002 , G02B21/0076 , G02B21/16 , G02B27/0927 , G02B27/0983
摘要： A system and method of high-speed microscopy using a two-photon microscope with spectral resolution. The microscope is operable to provide spectrally resolved, multi-dimensional images from a single scan of a sample. The microscope may include one of a multi-beam point scanning microscope, a single beam line scanning microscope, and a multi-beam line scanning microscope. The microscope includes a descanning arrangement such that emitted fluorescence is static on a receiving detector. The detector is a narrow detector with a width at least half the size of the length, to reduce the amount of pixel data being transmitted and improve scan speeds. The microscope may also incorporate one or more binning techniques whereby pixels are binned together to improve resolution or scan speeds.
摘要翻译： 使用具有光谱分辨率的双光子显微镜的高速显微镜的系统和方法。 显微镜可操作以从样品的单次扫描提供光谱分辨的多维图像。 显微镜可以包括多光束点扫描显微镜，单束线扫描显微镜和多光束线扫描显微镜中的一个。 显微镜包括一种下拉排列，使得发射的荧光在接收检测器上是静态的。 检测器是窄度检测器，其宽度至少为长度的一半，以减少被传输的像素数据量并提高扫描速度。 显微镜还可以包含一个或多个分档技术，由此将像素合并在一起以提高分辨率或扫描速度。
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公开(公告)号：US20120257038A1
公开(公告)日：2012-10-11
申请号：US13439958
申请日：2012-04-05
申请人： Valerica Raicu , Michael Stoneman
发明人： Valerica Raicu , Michael Stoneman
CPC分类号： G01J3/2803 , G01J3/027 , G01J3/0294 , G01J3/06 , G01J3/4406 , G01N15/1434 , G01N15/1463 , G01N15/1475 , G01N2015/1006 , G02B21/002 , G02B21/0076 , G02B21/16 , G02B27/0927 , G02B27/0983
摘要： A system and method of high-speed microscopy using a two-photon microscope with spectral resolution. The microscope is operable to provide spectrally resolved, multi-dimensional images from a single scan of a sample. The microscope may include one of a multi-beam point scanning microscope, a single beam line scanning microscope, and a multi-beam line scanning microscope. The microscope includes a descanning arrangement such that emitted fluorescence is static on a receiving detector. The detector is a narrow detector with a width at least half the size of the length, to reduce the amount of pixel data being transmitted and improve scan speeds. The microscope may also incorporate one or more binning techniques whereby pixels are binned together to improve resolution or scan speeds.
摘要翻译： 使用具有光谱分辨率的双光子显微镜的高速显微镜的系统和方法。 显微镜可操作以从样品的单次扫描提供光谱分辨的多维图像。 显微镜可以包括多光束点扫描显微镜，单束线扫描显微镜和多光束线扫描显微镜中的一个。 显微镜包括一种下拉排列，使得发射的荧光在接收检测器上是静态的。 检测器是窄度检测器，其宽度至少为长度的一半，以减少被传输的像素数据量并提高扫描速度。 显微镜还可以包含一个或多个分档技术，由此将像素合并在一起以提高分辨率或扫描速度。
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