公开(公告)号：US20230272346A1

公开(公告)日：2023-08-31

申请号：US18046083

申请日：2022-10-12

Applicant: Upside Foods, Inc.

Inventor： Nicholas J. GENOVESE ,  Meri Teresa FIRPO ,  Daphné DAMBOURNET

IPC: C12N5/077 ,  C07K14/47

CPC classification number: C12N5/0658 ,  C07K14/4703 ,  C12N5/0656 ,  C12N2501/998 ,  C12N2511/00

Abstract: Provided herein are methods to increase the culture density and/or thickness of a cellular biomass in a cultivation infrastructure, to improve the culture of cells in the absence of serum in a cultivation infrastructure, and to promote anchorage-independent growth of a cellular biomass in a cultivation infrastructure. The methods comprise inhibiting the HIPPO signaling pathway, for example, by activating YAP1, activating TAZ, and/or inhibiting MOB1, LATS1 kinase, LATS2 kinase, WW45, MST1 kinase, and/or MST2 kinase in the cellular biomass. In some embodiments, the cellular biomass is harvested from the cultivation infrastructure for the formulation of cell-based food products or ingredients, such as animal meat manufactured from cells in an ex vivo process or for therapeutic applications such as organ or tissue transplantation or grafting.

公开(公告)号：US20240318133A1

公开(公告)日：2024-09-26

申请号：US18731343

申请日：2024-06-02

Applicant: Upside Foods, Inc.

Inventor： Kalle Lukyan JOHNSON ,  Patricia R. BENTON ,  Cameron Gentry COPELAND ,  Sukhdeep Singh DHADWAR ,  Meri Teresa FIRPO ,  Marie Elizabeth GIBBONS ,  Stephen K. HSU ,  Robert SIERRA ,  Kevin John KAYSER

IPC: C12N5/071

CPC classification number: C12N5/0602 ,  C12N2500/14 ,  C12N2500/24 ,  C12N2500/32 ,  C12N2500/34 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2501/105 ,  C12N2501/11 ,  C12N2501/113 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/12 ,  C12N2501/135 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/33

Abstract: Provided herein are nutrient media formulations and engineered growth factors, and methods thereof, useful for the production of slaughter-free meat.

公开(公告)号：US20220073870A1

公开(公告)日：2022-03-10

申请号：US17341220

申请日：2021-06-07

Applicant: Upside Foods, Inc.

Inventor： Kalle Lukyan JOHNSON ,  Patricia R. BENTON ,  Cameron Gentry COPELAND ,  Sukhdeep Singh DHADWAR ,  Meri Teresa FIRPO ,  Marie Elizabeth GIBBONS ,  Stephen K. HSU ,  Robert SIERRA ,  Kevin John KAYSER

IPC: C12N5/071

Abstract: Provided herein are nutrient media formulations and engineered growth factors, and methods thereof, useful for the production of slaughter-free meat.