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    METHODS FOR EXTENDING THE REPLICATIVE CAPACITY OF SOMATIC CELLS DURING AN EX VIVO CULTIVATION PROCESS
    2.
    发明申请
    METHODS FOR EXTENDING THE REPLICATIVE CAPACITY OF SOMATIC CELLS DURING AN EX VIVO CULTIVATION PROCESS 有权

    公开(公告)号：US20220251550A1

    公开(公告)日：2022-08-11

    申请号：US17545632

    申请日：2021-12-08

    Applicant: Upside Foods, Inc.

    Inventor： Nicholas J. GENOVESE ,  Danielle Nicole DESMET ,  Eric SCHULZE

    IPC: C12N15/11 ,  C12N9/12 ,  C12N5/00 ,  A23L33/135 ,  C12N5/077 ,  C12N9/22

    Abstract: A product and process for extending the replicative capacity of metazoan somatic cells using targeted genetic amendments to abrogate inhibition of cell-cycle progression during replicative senescence and derive clonal cell lines for scalable applications and industrial production of metazoan cell biomass. An insertion or deletion mutation using guide RNAs targeting the first exon of the transcript encoding each protein is created using CRISPR/Cas9. Targeted amendments result in inactivation of p15 and p16 proteins which increases the proliferative capacity of the modified cell populations relative to their unaltered parental populations. Combining these amendments with ancillary telomerase activity from a genetic construct directing expression of a telomerase protein homolog from a TERT gene, increases the replicative capacity of the modified cell populations indefinitely. One application is to manufacture skeletal muscle for dietary consumption using cells from the poultry species Gallus gallus; another is from the livestock species Bos taurus.

    SUBSTRATES AND BIOREACTORS FOR PREPARING A COMESTIBLE MEAT PRODUCT
    5.
    发明申请
    SUBSTRATES AND BIOREACTORS FOR PREPARING A COMESTIBLE MEAT PRODUCT 有权

    公开(公告)号：US20230091040A1

    公开(公告)日：2023-03-23

    申请号：US18060322

    申请日：2022-11-30

    Applicant: Upside Foods, Inc.

    Inventor： Matthew LEUNG ,  Asha GODBOLE ,  George C. ENGELMAYR, JR. ,  Nicholas J. GENOVESE ,  Uma S. VALETI ,  Kathleen CARSWELL

    IPC: A23L13/50 ,  C12M3/00 ,  C12N5/077

    Abstract: The substrates, systems, and methods described herein relate to textured substrates for preparing a comestible meat product. Substrates and methods are described herein for controlling one or more of growth, adhesion, retention, and/or release of cells (e.g., of a cell sheet) on or from the surface of the substrate. A method of preparing a comestible meat product may include applying a plurality of non-human cells to at least one patterned texture substrate, growing the cells on the patterned texture substrate to form the comestible meat product, and separating the comestible meat product from the patterned texture substrate. The patterned texture allows for improved growth, adhesion, retention, and/or release of cells as compared to another surface not comprising the patterned texture. In some embodiments, the cell culture substrate surfaces include a plurality of regions corresponding to a plurality of patterned textures.

    FLUID CONDUIT WITH RADIAL EXPANSION OF FLUID FLOW
    6.
    发明申请
    FLUID CONDUIT WITH RADIAL EXPANSION OF FLUID FLOW 有权

    公开(公告)号：US20220195368A1

    公开(公告)日：2022-06-23

    申请号：US17129662

    申请日：2020-12-21

    Applicant: Upside Foods, Inc.

    Inventor： Nicholas J. GENOVESE

    IPC: C12M1/00 ,  B01D69/02 ,  B01D63/08 ,  B01D69/06

    Abstract: Disclosed herein are systems, devices, and methods for flowing fluid for radially expanded particle distribution within a laminar flow. In some variations, a system for cultivating tissue may comprise a bioreactor comprising an inlet, a substrate arranged in the bioreactor, and a diffusion module configured to transfer fluid from the inlet to the substrate. The diffusion module may comprise a porous material having at least one tortuous conduit extending between a first surface of the porous material and a second surface of the porous material.

    SUBSTRATES FOR PREPARING A COMESTIBLE MEAT PRODUCT
    7.
    发明申请
    SUBSTRATES FOR PREPARING A COMESTIBLE MEAT PRODUCT 有权

    公开(公告)号：US20220248728A1

    公开(公告)日：2022-08-11

    申请号：US17660170

    申请日：2022-04-21

    Applicant: Upside Foods, Inc.

    Inventor： Matthew LEUNG ,  Asha GODBOLE ,  George C. ENGELMAYR, JR. ,  Nicholas J. GENOVESE ,  Uma S. VALETI ,  Kathleen CARSWELL

    IPC: A23L13/50 ,  C12M3/00 ,  C12N5/077

    Abstract: The substrates, systems, and methods described herein relate to textured substrates for preparing a comestible meat product. Substrates and methods are described herein for controlling one or more of growth, adhesion, retention, and/or release of cells (e.g., of a cell sheet) on or from the surface of the substrate. A method of preparing a comestible meat product may include applying a plurality of non-human cells to at least one patterned texture substrate, growing the cells on the patterned texture substrate to form the comestible meat product, and separating the comestible meat product from the patterned texture substrate. The patterned texture allows for improved growth, adhesion, retention, and/or release of cells as compared to another surface not comprising the patterned texture. In some embodiments, the cell culture substrate surfaces include a plurality of regions corresponding to a plurality of patterned textures.

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